Click here to switch to the map view.

The map label for this gene is leuA [H]

Identifier: 238917154

GI number: 238917154

Start: 1242767

End: 1244428

Strand: Reverse

Name: leuA [H]

Synonym: EUBELI_01226

Alternate gene names: 238917154

Gene position: 1244428-1242767 (Counterclockwise)

Preceding gene: 238917155

Following gene: 238917153

Centisome position: 58.04

GC content: 38.81

Gene sequence:

>1662_bases
ATGTTTGATTACAGACAGTATGAAAGAGGCTATTTTATGCCACCGGTTAAGTGCAATGACTGGGTTAACAAGGAGTATGT
TGATAAGGCACCAATCTGGTGTTCGGTTGATTTAAGAGATGGTAACCAGGCTCTTGTTGAACCTATGAGCCTTGATGAAA
AGCTGGAATTCTTCCAGATGTTAGTTGAAGTCGGATTTAAGGAAATTGAGATAGGTTTCCCTGCAGCTTCTGAGACAGAA
TATGAATTCTGCCGTACACTTATTGAGAAGAATATGATTCCTGATGATGTTACAATACAGGTACTTACACAGGCAAGACC
ACATATTATTAAGAAGACATTTGAAGCTGTAAAGGGTGCTCCAAGAGCAATCGTTCATGTATATAATTCTACTTCGTTAG
CTCAGAGAGAGCAGGTGTTTAAGAAGTCTAAAGAAGAGGTTAAACAGATTGCTATTGAAGGTGCTAAGTTATTAAAGGAG
CTTGCAGAAGCTGATGGTGGAAACTTCCTTTTTGAATACAGTCCGGAGAGCTTTACAGGAACTGAGCCGGAGTATGCGCT
TGAAGTGTGCAACGCTGTTGTTGATATCTGGCAGCCAACACCTGATAAGAAATGTATCATCAATCTTCCGGCTACAGTTG
AGATGTCACTTCCGCATGTATTTGCAAGCCAGATTGAATATATGTCTAAGTACATGCACAACAGAGAAAATGTTATTATT
TCTCTTCATCCACATAATGACCGTTCGACAGGTGTTGCTGATGCAGAGTTAGGACTTCTTGCAGGAGCAGACAGAATTGA
GGGAACACTTTTTGGTAATGGTGAGAGAACAGGTAATGTAGATATTATTACACTTGCTATGAATATGTATATGCAGGGTG
TTAATCCTGAGCTTGATCTTTCTGATATGCCACATCTTGCAGAAGTGTTTGAAAGACTCACACAGATGAAGATTGATGAC
CGTCATCCATGGTGTGGTAAGCTTGTTTTTGCAGCTTTCTCAGGTTCACATCAGGATGCTATATCTAAGGGAATGCATTA
CAGAATTGAAAAAGATCCTAATAAATGGACAGTTCCATATCTTCCAATTAATCCAGAAGATGTCGGACGAACATATGATT
CAGATGTTATCAGAATTAACAGCCAGTCAGGCAAGGGCGGTGTAGCATATGTCCTTGAGCATAATTATGGAATGATAGTT
CCTAAGGCTATGAGAGAAGATTTAGGATATGCAGTTAAGGATGTTTCTGATGTACATCATAAGGAACTCTCACCAGATGA
AGTTCTTGAAATTTTCGAGAAGAGATATAAAAAGTACACACCGGTATTTAAGATTACGGAAGTACATTTTAAGCAGATTA
ACGGTATCCAGACTGTTGTGACTATTGATGAAAACGGAAAGAAGAGCAATGTTGAGAACGGTGGTAATGGCCGTCTTGAT
GCAGTAAGCAATGCTCTTTCAGAACATTTTGGAAAGCCTTGTGATATTTTCTGTTATGAAGAACATTCATTAAAGAAGGG
ATCTGATTCAAGTGCTATTGCGTATGTTGGTATTACAGGAGAAGATGGTAAGAATTACTTTGGTATTGGTATTGATCACG
ATATTATCAGAGCTTCTATTGATGCATTAGAGTCAGCAATGAACAGAAGTATTGAGGCATAA

Upstream 100 bases:

>100_bases
CTTTGGGTAGAATTACCCATGGACGAAAGATTTTTTTATGCTTATCTTTGAGAAAGCTTTTACTTATTAACCATATATAT
TTTTTCAGGAGGTTTTAGGA

Downstream 100 bases:

>100_bases
TTTATTGTTTAATTAAATGATTTGTAATATAACTCATGTCCTGGACATATACTAGAATGATAGTGGCCCGGGGCATGAGT
TATTTTTTTTATGTAAAGTT

Product: 2-isopropylmalate synthase

Products: NA

Alternate protein names: Alpha-IPM synthase; Alpha-isopropylmalate synthase [H]

Number of amino acids: Translated: 553; Mature: 553

Protein sequence:

>553_residues
MFDYRQYERGYFMPPVKCNDWVNKEYVDKAPIWCSVDLRDGNQALVEPMSLDEKLEFFQMLVEVGFKEIEIGFPAASETE
YEFCRTLIEKNMIPDDVTIQVLTQARPHIIKKTFEAVKGAPRAIVHVYNSTSLAQREQVFKKSKEEVKQIAIEGAKLLKE
LAEADGGNFLFEYSPESFTGTEPEYALEVCNAVVDIWQPTPDKKCIINLPATVEMSLPHVFASQIEYMSKYMHNRENVII
SLHPHNDRSTGVADAELGLLAGADRIEGTLFGNGERTGNVDIITLAMNMYMQGVNPELDLSDMPHLAEVFERLTQMKIDD
RHPWCGKLVFAAFSGSHQDAISKGMHYRIEKDPNKWTVPYLPINPEDVGRTYDSDVIRINSQSGKGGVAYVLEHNYGMIV
PKAMREDLGYAVKDVSDVHHKELSPDEVLEIFEKRYKKYTPVFKITEVHFKQINGIQTVVTIDENGKKSNVENGGNGRLD
AVSNALSEHFGKPCDIFCYEEHSLKKGSDSSAIAYVGITGEDGKNYFGIGIDHDIIRASIDALESAMNRSIEA

Sequences:

>Translated_553_residues
MFDYRQYERGYFMPPVKCNDWVNKEYVDKAPIWCSVDLRDGNQALVEPMSLDEKLEFFQMLVEVGFKEIEIGFPAASETE
YEFCRTLIEKNMIPDDVTIQVLTQARPHIIKKTFEAVKGAPRAIVHVYNSTSLAQREQVFKKSKEEVKQIAIEGAKLLKE
LAEADGGNFLFEYSPESFTGTEPEYALEVCNAVVDIWQPTPDKKCIINLPATVEMSLPHVFASQIEYMSKYMHNRENVII
SLHPHNDRSTGVADAELGLLAGADRIEGTLFGNGERTGNVDIITLAMNMYMQGVNPELDLSDMPHLAEVFERLTQMKIDD
RHPWCGKLVFAAFSGSHQDAISKGMHYRIEKDPNKWTVPYLPINPEDVGRTYDSDVIRINSQSGKGGVAYVLEHNYGMIV
PKAMREDLGYAVKDVSDVHHKELSPDEVLEIFEKRYKKYTPVFKITEVHFKQINGIQTVVTIDENGKKSNVENGGNGRLD
AVSNALSEHFGKPCDIFCYEEHSLKKGSDSSAIAYVGITGEDGKNYFGIGIDHDIIRASIDALESAMNRSIEA
>Mature_553_residues
MFDYRQYERGYFMPPVKCNDWVNKEYVDKAPIWCSVDLRDGNQALVEPMSLDEKLEFFQMLVEVGFKEIEIGFPAASETE
YEFCRTLIEKNMIPDDVTIQVLTQARPHIIKKTFEAVKGAPRAIVHVYNSTSLAQREQVFKKSKEEVKQIAIEGAKLLKE
LAEADGGNFLFEYSPESFTGTEPEYALEVCNAVVDIWQPTPDKKCIINLPATVEMSLPHVFASQIEYMSKYMHNRENVII
SLHPHNDRSTGVADAELGLLAGADRIEGTLFGNGERTGNVDIITLAMNMYMQGVNPELDLSDMPHLAEVFERLTQMKIDD
RHPWCGKLVFAAFSGSHQDAISKGMHYRIEKDPNKWTVPYLPINPEDVGRTYDSDVIRINSQSGKGGVAYVLEHNYGMIV
PKAMREDLGYAVKDVSDVHHKELSPDEVLEIFEKRYKKYTPVFKITEVHFKQINGIQTVVTIDENGKKSNVENGGNGRLD
AVSNALSEHFGKPCDIFCYEEHSLKKGSDSSAIAYVGITGEDGKNYFGIGIDHDIIRASIDALESAMNRSIEA

Specific function: Catalyzes the condensation of the acetyl group of acetyl-CoA with 3-methyl-2-oxobutanoate (2-oxoisovalerate) to form 3-carboxy-3-hydroxy-4-methylpentanoate (2-isopropylmalate) [H]

COG id: COG0119

COG function: function code E; Isopropylmalate/homocitrate/citramalate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alpha-IPM synthase/homocitrate synthase family. LeuA type 2 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786261, Length=518, Percent_Identity=23.5521235521236, Blast_Score=108, Evalue=9e-25,
Organism=Saccharomyces cerevisiae, GI6324682, Length=560, Percent_Identity=43.0357142857143, Blast_Score=462, Evalue=1e-131,
Organism=Saccharomyces cerevisiae, GI6324225, Length=561, Percent_Identity=42.0677361853832, Blast_Score=457, Evalue=1e-129,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013709
- InterPro:   IPR002034
- InterPro:   IPR013785
- InterPro:   IPR005668
- InterPro:   IPR000891 [H]

Pfam domain/function: PF00682 HMGL-like; PF08502 LeuA_dimer [H]

EC number: =2.3.3.13 [H]

Molecular weight: Translated: 62289; Mature: 62289

Theoretical pI: Translated: 4.89; Mature: 4.89

Prosite motif: PS00815 AIPM_HOMOCIT_SYNTH_1 ; PS50991 PYR_CT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFDYRQYERGYFMPPVKCNDWVNKEYVDKAPIWCSVDLRDGNQALVEPMSLDEKLEFFQM
CCCCHHHCCCCCCCCCCCCCCCCCHHHCCCCEEEEEEECCCCHHHHCCCCHHHHHHHHHH
LVEVGFKEIEIGFPAASETEYEFCRTLIEKNMIPDDVTIQVLTQARPHIIKKTFEAVKGA
HHHCCCEEEEECCCCCCCCHHHHHHHHHHCCCCCCCCEEEEHHHCCHHHHHHHHHHHCCC
PRAIVHVYNSTSLAQREQVFKKSKEEVKQIAIEGAKLLKELAEADGGNFLFEYSPESFTG
CEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCC
TEPEYALEVCNAVVDIWQPTPDKKCIINLPATVEMSLPHVFASQIEYMSKYMHNRENVII
CCHHHHHHHHHHHHHHCCCCCCCCEEEECCCEEEECCHHHHHHHHHHHHHHHCCCCCEEE
SLHPHNDRSTGVADAELGLLAGADRIEGTLFGNGERTGNVDIITLAMNMYMQGVNPELDL
EEECCCCCCCCCCHHHHHHEECCCCCCEEEEECCCCCCCEEEEEEEHHHHHCCCCCCCCC
SDMPHLAEVFERLTQMKIDDRHPWCGKLVFAAFSGSHQDAISKGMHYRIEKDPNKWTVPY
CCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCHHHHHCCCEEEEECCCCEEEEEE
LPINPEDVGRTYDSDVIRINSQSGKGGVAYVLEHNYGMIVPKAMREDLGYAVKDVSDVHH
ECCCHHHHCCCCCCCEEEEECCCCCCCEEEEEECCCCEEECHHHHHHHCHHHHHHHHHHH
KELSPDEVLEIFEKRYKKYTPVFKITEVHFKQINGIQTVVTIDENGKKSNVENGGNGRLD
CCCCHHHHHHHHHHHHHHCCCEEEEHHHHHHHCCCEEEEEEECCCCCCCCCCCCCCCCHH
AVSNALSEHFGKPCDIFCYEEHSLKKGSDSSAIAYVGITGEDGKNYFGIGIDHDIIRASI
HHHHHHHHHCCCCCEEEEECCCCCCCCCCCCEEEEEEEECCCCCEEEECCCCHHHHHHHH
DALESAMNRSIEA
HHHHHHHHCCCCC
>Mature Secondary Structure
MFDYRQYERGYFMPPVKCNDWVNKEYVDKAPIWCSVDLRDGNQALVEPMSLDEKLEFFQM
CCCCHHHCCCCCCCCCCCCCCCCCHHHCCCCEEEEEEECCCCHHHHCCCCHHHHHHHHHH
LVEVGFKEIEIGFPAASETEYEFCRTLIEKNMIPDDVTIQVLTQARPHIIKKTFEAVKGA
HHHCCCEEEEECCCCCCCCHHHHHHHHHHCCCCCCCCEEEEHHHCCHHHHHHHHHHHCCC
PRAIVHVYNSTSLAQREQVFKKSKEEVKQIAIEGAKLLKELAEADGGNFLFEYSPESFTG
CEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCC
TEPEYALEVCNAVVDIWQPTPDKKCIINLPATVEMSLPHVFASQIEYMSKYMHNRENVII
CCHHHHHHHHHHHHHHCCCCCCCCEEEECCCEEEECCHHHHHHHHHHHHHHHCCCCCEEE
SLHPHNDRSTGVADAELGLLAGADRIEGTLFGNGERTGNVDIITLAMNMYMQGVNPELDL
EEECCCCCCCCCCHHHHHHEECCCCCCEEEEECCCCCCCEEEEEEEHHHHHCCCCCCCCC
SDMPHLAEVFERLTQMKIDDRHPWCGKLVFAAFSGSHQDAISKGMHYRIEKDPNKWTVPY
CCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCHHHHHCCCEEEEECCCCEEEEEE
LPINPEDVGRTYDSDVIRINSQSGKGGVAYVLEHNYGMIVPKAMREDLGYAVKDVSDVHH
ECCCHHHHCCCCCCCEEEEECCCCCCCEEEEEECCCCEEECHHHHHHHCHHHHHHHHHHH
KELSPDEVLEIFEKRYKKYTPVFKITEVHFKQINGIQTVVTIDENGKKSNVENGGNGRLD
CCCCHHHHHHHHHHHHHHCCCEEEEHHHHHHHCCCEEEEEEECCCCCCCCCCCCCCCCHH
AVSNALSEHFGKPCDIFCYEEHSLKKGSDSSAIAYVGITGEDGKNYFGIGIDHDIIRASI
HHHHHHHHHCCCCCEEEEECCCCCCCCCCCCEEEEEEEECCCCCEEEECCCCHHHHHHHH
DALESAMNRSIEA
HHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12810954 [H]