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The map label for this gene is iscS [H]

Identifier: 238917123

GI number: 238917123

Start: 1208033

End: 1209163

Strand: Reverse

Name: iscS [H]

Synonym: EUBELI_01194

Alternate gene names: 238917123

Gene position: 1209163-1208033 (Counterclockwise)

Preceding gene: 238917124

Following gene: 238917122

Centisome position: 56.39

GC content: 40.58

Gene sequence:

>1131_bases
ATGATATATATGGACAATGCGGCGACAACTGCTATCAGAAAAGAAGTGGCTGAAGCAATGCTTCCATATCTTTTGGGAGA
TTATGGAAACCCGTCAGGTGTGTATAGGTTGTCTGAAAAAGCTAAAAATGTTGTAGAGGAATCAAGAGGTATTATTGCAG
ATATAATAAATGCTGACAGAAATGAAATATTTTTCACCTCTGGTGGAACGGAAGGGGATAACTGGGCAGTTAAAGCAGAG
AGTCTGAAAAGAAAACAGGGACATATAATAGTAAGTTCAATAGAACACCATGCGGTGACGGAATCGGCACTGTGGCTTAA
GAATTATGGAATAGATGTCACATTTGCACCAGTTGATGAGCAGGGAATTGTTAAGCTGGAACAGTTAGAAAAAATGATTA
GAAATGATACATTTTTAGTGTCAGTTATGCTTGCTAATAATGAAATTGGAACTATCCAGCCGATAGAAAATGTATGTGAA
ATTGCGCATGAGTATGGTGCAAAGGTTCATTCTGATGCAGTGGCGGCATTTGGACATATTGATGTTGATGTGAAAAAGCT
GGGTGTGGATTTCTTAAATGTAAGCGGACATAAGATATGCGGACCAAAGGGCGTAGGATTTCTGTATGTCAGGGGCGGAA
AGCCAGAACGGTTCATGCATGGAGGCGGACAGGAGAACGGACTTAGGGCAGGCACTGAAAATGTAGCTGGGATTGCAGGA
CTTGCAACAGCAGCAAGAATTGCTTATGAAACTCTTTCTGACAGGGCAAGGCAGGAAAAGAGAATTAGTGACTATATGAT
TAAGAGAATACTAAGAGAAATTCCTTATTCAAGGCTTAATGGAAGCAGGGAATGCCGCGTTACAGGTAATATGAATTTCA
GCTTTCAGTATGTTAATGGCGGAATGCTTGTTGTAATGCTTAATAAGCAGGGGATATGCGTGTCTGCAGGTTCAGCATGT
ACATCTAATTCAGGAAAGCCATCACATGTCCTTCTTGCACTTGGACTTGATGAAAAAATAGCAGAAGGTTCACTAAGACT
GACAATTAACCATACAATTACACACAGGGAAGCTGATTATGTGGTTGAGTGTATTAAGAAAGATGTTGCCAGACTGCGGG
AATTGGCTTAA

Upstream 100 bases:

>100_bases
GGATAATTTATTATTCATAGCAGATACCGCAGATAGACTTATTAACAATATATGCCCTGCCTGAATACAATATTATAAGG
AATTACAGGGCGGAAATATA

Downstream 100 bases:

>100_bases
TATTAATATATCAGACAGTTACGGAGGTTAAAATAATATGTGGTACGAAGAAGCGGTGTTTTATCAGGTATTTCCACTTG
GATTTTGTGATGGATTAAGA

Product: cysteine desulfurase

Products: NA

Alternate protein names: NifS protein homolog [H]

Number of amino acids: Translated: 376; Mature: 376

Protein sequence:

>376_residues
MIYMDNAATTAIRKEVAEAMLPYLLGDYGNPSGVYRLSEKAKNVVEESRGIIADIINADRNEIFFTSGGTEGDNWAVKAE
SLKRKQGHIIVSSIEHHAVTESALWLKNYGIDVTFAPVDEQGIVKLEQLEKMIRNDTFLVSVMLANNEIGTIQPIENVCE
IAHEYGAKVHSDAVAAFGHIDVDVKKLGVDFLNVSGHKICGPKGVGFLYVRGGKPERFMHGGGQENGLRAGTENVAGIAG
LATAARIAYETLSDRARQEKRISDYMIKRILREIPYSRLNGSRECRVTGNMNFSFQYVNGGMLVVMLNKQGICVSAGSAC
TSNSGKPSHVLLALGLDEKIAEGSLRLTINHTITHREADYVVECIKKDVARLRELA

Sequences:

>Translated_376_residues
MIYMDNAATTAIRKEVAEAMLPYLLGDYGNPSGVYRLSEKAKNVVEESRGIIADIINADRNEIFFTSGGTEGDNWAVKAE
SLKRKQGHIIVSSIEHHAVTESALWLKNYGIDVTFAPVDEQGIVKLEQLEKMIRNDTFLVSVMLANNEIGTIQPIENVCE
IAHEYGAKVHSDAVAAFGHIDVDVKKLGVDFLNVSGHKICGPKGVGFLYVRGGKPERFMHGGGQENGLRAGTENVAGIAG
LATAARIAYETLSDRARQEKRISDYMIKRILREIPYSRLNGSRECRVTGNMNFSFQYVNGGMLVVMLNKQGICVSAGSAC
TSNSGKPSHVLLALGLDEKIAEGSLRLTINHTITHREADYVVECIKKDVARLRELA
>Mature_376_residues
MIYMDNAATTAIRKEVAEAMLPYLLGDYGNPSGVYRLSEKAKNVVEESRGIIADIINADRNEIFFTSGGTEGDNWAVKAE
SLKRKQGHIIVSSIEHHAVTESALWLKNYGIDVTFAPVDEQGIVKLEQLEKMIRNDTFLVSVMLANNEIGTIQPIENVCE
IAHEYGAKVHSDAVAAFGHIDVDVKKLGVDFLNVSGHKICGPKGVGFLYVRGGKPERFMHGGGQENGLRAGTENVAGIAG
LATAARIAYETLSDRARQEKRISDYMIKRILREIPYSRLNGSRECRVTGNMNFSFQYVNGGMLVVMLNKQGICVSAGSAC
TSNSGKPSHVLLALGLDEKIAEGSLRLTINHTITHREADYVVECIKKDVARLRELA

Specific function: Catalyzes the removal of elemental sulfur from cysteine to produce alanine [H]

COG id: COG1104

COG function: function code E; Cysteine sulfinate desulfinase/cysteine desulfurase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. NifS/IscS subfamily [H]

Homologues:

Organism=Homo sapiens, GI32307132, Length=382, Percent_Identity=40.0523560209424, Blast_Score=276, Evalue=2e-74,
Organism=Homo sapiens, GI156713448, Length=415, Percent_Identity=32.7710843373494, Blast_Score=200, Evalue=2e-51,
Organism=Escherichia coli, GI48994898, Length=384, Percent_Identity=41.1458333333333, Blast_Score=273, Evalue=1e-74,
Organism=Escherichia coli, GI1787970, Length=346, Percent_Identity=28.9017341040462, Blast_Score=115, Evalue=5e-27,
Organism=Escherichia coli, GI1789175, Length=396, Percent_Identity=27.5252525252525, Blast_Score=105, Evalue=4e-24,
Organism=Caenorhabditis elegans, GI25143064, Length=384, Percent_Identity=41.1458333333333, Blast_Score=274, Evalue=6e-74,
Organism=Caenorhabditis elegans, GI17533177, Length=324, Percent_Identity=29.9382716049383, Blast_Score=129, Evalue=2e-30,
Organism=Saccharomyces cerevisiae, GI6319831, Length=382, Percent_Identity=42.6701570680628, Blast_Score=283, Evalue=3e-77,
Organism=Drosophila melanogaster, GI20129463, Length=382, Percent_Identity=40.0523560209424, Blast_Score=267, Evalue=1e-71,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000192
- InterPro:   IPR010240
- InterPro:   IPR017772
- InterPro:   IPR016454
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00266 Aminotran_5 [H]

EC number: =2.8.1.7 [H]

Molecular weight: Translated: 41169; Mature: 41169

Theoretical pI: Translated: 7.09; Mature: 7.09

Prosite motif: PS00595 AA_TRANSFER_CLASS_5

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIYMDNAATTAIRKEVAEAMLPYLLGDYGNPSGVYRLSEKAKNVVEESRGIIADIINADR
CEEECCHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEHHHHHHHHHHHHCCCEEEHHCCCC
NEIFFTSGGTEGDNWAVKAESLKRKQGHIIVSSIEHHAVTESALWLKNYGIDVTFAPVDE
CEEEEECCCCCCCCEEEEHHHHHCCCCCEEEEECHHHHHHHHHHHHHHCCCEEEEECCCC
QGIVKLEQLEKMIRNDTFLVSVMLANNEIGTIQPIENVCEIAHEYGAKVHSDAVAAFGHI
CCCEEHHHHHHHHCCCEEEEEEEEECCCCCCCCHHHHHHHHHHHHCCCHHCCHHHHHCCC
DVDVKKLGVDFLNVSGHKICGPKGVGFLYVRGGKPERFMHGGGQENGLRAGTENVAGIAG
CEEHHHHCCEEEECCCCEEECCCCCEEEEEECCCCHHHHCCCCCCCCCCCCCCCHHHHHH
LATAARIAYETLSDRARQEKRISDYMIKRILREIPYSRLNGSRECRVTGNMNFSFQYVNG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCCEEEEEECCEEEEEEECC
GMLVVMLNKQGICVSAGSACTSNSGKPSHVLLALGLDEKIAEGSLRLTINHTITHREADY
CEEEEEECCCCEEEECCCCCCCCCCCCCEEEEEECCCCHHCCCEEEEEEEEEEECCHHHH
VVECIKKDVARLRELA
HHHHHHHHHHHHHHCC
>Mature Secondary Structure
MIYMDNAATTAIRKEVAEAMLPYLLGDYGNPSGVYRLSEKAKNVVEESRGIIADIINADR
CEEECCHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEHHHHHHHHHHHHCCCEEEHHCCCC
NEIFFTSGGTEGDNWAVKAESLKRKQGHIIVSSIEHHAVTESALWLKNYGIDVTFAPVDE
CEEEEECCCCCCCCEEEEHHHHHCCCCCEEEEECHHHHHHHHHHHHHHCCCEEEEECCCC
QGIVKLEQLEKMIRNDTFLVSVMLANNEIGTIQPIENVCEIAHEYGAKVHSDAVAAFGHI
CCCEEHHHHHHHHCCCEEEEEEEEECCCCCCCCHHHHHHHHHHHHCCCHHCCHHHHHCCC
DVDVKKLGVDFLNVSGHKICGPKGVGFLYVRGGKPERFMHGGGQENGLRAGTENVAGIAG
CEEHHHHCCEEEECCCCEEECCCCCEEEEEECCCCHHHHCCCCCCCCCCCCCCCHHHHHH
LATAARIAYETLSDRARQEKRISDYMIKRILREIPYSRLNGSRECRVTGNMNFSFQYVNG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCCEEEEEECCEEEEEEECC
GMLVVMLNKQGICVSAGSACTSNSGKPSHVLLALGLDEKIAEGSLRLTINHTITHREADY
CEEEEEECCCCEEEECCCCCCCCCCCCCEEEEEECCCCHHCCCEEEEEEEEEEECCHHHH
VVECIKKDVARLRELA
HHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10930739 [H]