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The map label for this gene is pemA [H]

Identifier: 238917104

GI number: 238917104

Start: 1186880

End: 1187821

Strand: Direct

Name: pemA [H]

Synonym: EUBELI_01174

Alternate gene names: 238917104

Gene position: 1186880-1187821 (Clockwise)

Preceding gene: 238917101

Following gene: 238917121

Centisome position: 55.35

GC content: 38.64

Gene sequence:

>942_bases
ATGATTACTGTAGCTAAAGATAATTCCGGAGATTTTAATTCTATCCAGCAGGCTGTTGATTCAATTCCAGCCGGAACCCC
TGAAACTATCTATATCAAAAAAGGAATATATAAAGAACGCGTTGAAGTCAGAAAGAATAATATATCATTTGTTGGTGAAA
GCACTGATGATACTATAATAACAGAAAGCTATTATGCAAGAATGATTATGCCTGATGGCTCTAAACGTGGAACTTTCCGT
TCTTATACATTTTTCGTCTACGCAGACAATTTTACTGCCAGCAATTTAACTTTTGAAAATGCAGCCGGATTCGGTGATGA
ATTTGGCCAGGCGATTGCAGTATATGCCGAAGGGGATAACATAACTTTCAGGAACTGCAAAATTCTCGGTCATCAGGATA
CTCTGTTCACCGGTCCTCTTCCAATGAAAGAAAAGCAGCCTGGTGGTTTCACCGGACCTACAATTGACGGAATACGCCGT
GTTGTCCACCAGTTATATGAAGACTGCTACATTGCAGGAGAAATTGATTTTATATTTGGAAGTGCTACTGCTTACTTTAA
AAACTGCACCCTTTTTGCACTGAACAGAAATCAGGAAATCAATGCTTTCTATACAGCACCTTCTACATATGAAGGACAGG
CTTTTGGTTATGTATTTGAGAGCTGTACATTTACAGGTAACTGTCCTCCAAAAAGTGTTGCTTTAAGCAGGCCATGGAGA
ATACATGCCAAAACAGTTCTTCTTAACTGCAGCTATTCCGATCAGATAATTGATGAAGGATTTACTGACTGGAACAAGCC
CGAATCGCATGAAACTGTATATTATGCAGAATACAACGGACATGGAGAAGGTTTTAAGCCTGAAAAAAGAGCAGCTTATG
TTCATCAGTTAAATGAATCAGAAGCTGCCCTCTATACTCTTGAAAATGTTATGAATTCCTAA

Upstream 100 bases:

>100_bases
TCTCTATTTCCTACAAATTTTTTAACGAATCGAATTATACTCGCTTTATTTATTAATGTCCAGTGTGCTATAATAGCAAA
AAAATATCGGAGGTACTCAC

Downstream 100 bases:

>100_bases
TTAATTGTTAATGAACCAAGCGATGTTCCAGATGATGTTGCCGTCATAGAAGACCCGGCTACTAAACTGGCATCGCTTTC
TTTTACCTGAAAAAGTACCA

Product: carbohydrate esterase family 8-like pectin methylesterase

Products: NA

Alternate protein names: PE A; Pectin methylesterase A [H]

Number of amino acids: Translated: 313; Mature: 313

Protein sequence:

>313_residues
MITVAKDNSGDFNSIQQAVDSIPAGTPETIYIKKGIYKERVEVRKNNISFVGESTDDTIITESYYARMIMPDGSKRGTFR
SYTFFVYADNFTASNLTFENAAGFGDEFGQAIAVYAEGDNITFRNCKILGHQDTLFTGPLPMKEKQPGGFTGPTIDGIRR
VVHQLYEDCYIAGEIDFIFGSATAYFKNCTLFALNRNQEINAFYTAPSTYEGQAFGYVFESCTFTGNCPPKSVALSRPWR
IHAKTVLLNCSYSDQIIDEGFTDWNKPESHETVYYAEYNGHGEGFKPEKRAAYVHQLNESEAALYTLENVMNS

Sequences:

>Translated_313_residues
MITVAKDNSGDFNSIQQAVDSIPAGTPETIYIKKGIYKERVEVRKNNISFVGESTDDTIITESYYARMIMPDGSKRGTFR
SYTFFVYADNFTASNLTFENAAGFGDEFGQAIAVYAEGDNITFRNCKILGHQDTLFTGPLPMKEKQPGGFTGPTIDGIRR
VVHQLYEDCYIAGEIDFIFGSATAYFKNCTLFALNRNQEINAFYTAPSTYEGQAFGYVFESCTFTGNCPPKSVALSRPWR
IHAKTVLLNCSYSDQIIDEGFTDWNKPESHETVYYAEYNGHGEGFKPEKRAAYVHQLNESEAALYTLENVMNS
>Mature_313_residues
MITVAKDNSGDFNSIQQAVDSIPAGTPETIYIKKGIYKERVEVRKNNISFVGESTDDTIITESYYARMIMPDGSKRGTFR
SYTFFVYADNFTASNLTFENAAGFGDEFGQAIAVYAEGDNITFRNCKILGHQDTLFTGPLPMKEKQPGGFTGPTIDGIRR
VVHQLYEDCYIAGEIDFIFGSATAYFKNCTLFALNRNQEINAFYTAPSTYEGQAFGYVFESCTFTGNCPPKSVALSRPWR
IHAKTVLLNCSYSDQIIDEGFTDWNKPESHETVYYAEYNGHGEGFKPEKRAAYVHQLNESEAALYTLENVMNS

Specific function: Involved in maceration and soft-rotting of plant tissue [H]

COG id: COG4677

COG function: function code G; Pectin methylesterase

Gene ontology:

Cell location: Secreted [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pectinesterase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012334
- InterPro:   IPR011050
- InterPro:   IPR018040
- InterPro:   IPR000070 [H]

Pfam domain/function: PF01095 Pectinesterase [H]

EC number: =3.1.1.11 [H]

Molecular weight: Translated: 35093; Mature: 35093

Theoretical pI: Translated: 4.76; Mature: 4.76

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MITVAKDNSGDFNSIQQAVDSIPAGTPETIYIKKGIYKERVEVRKNNISFVGESTDDTII
CEEEEECCCCCHHHHHHHHHHCCCCCCCEEEEECCCHHHHHHHCCCCEEEEECCCCCEEE
TESYYARMIMPDGSKRGTFRSYTFFVYADNFTASNLTFENAAGFGDEFGQAIAVYAEGDN
ECCEEEEEEECCCCCCCCEEEEEEEEEECCCEECCEEECCCCCCCCCCCCEEEEEECCCC
ITFRNCKILGHQDTLFTGPLPMKEKQPGGFTGPTIDGIRRVVHQLYEDCYIAGEIDFIFG
EEEEEEEEECCCCCEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCEEEEEEEEEEEE
SATAYFKNCTLFALNRNQEINAFYTAPSTYEGQAFGYVFESCTFTGNCPPKSVALSRPWR
CCEEEECCEEEEEECCCCCEEEEEECCCCCCCCEEEEEEECCEECCCCCCCCEECCCCEE
IHAKTVLLNCSYSDQIIDEGFTDWNKPESHETVYYAEYNGHGEGFKPEKRAAYVHQLNES
EEEEEEEEECCCCHHHHHCCCCCCCCCCCCCEEEEEEECCCCCCCCCHHHHHHHHHCCCC
EAALYTLENVMNS
CHHHHHHHHHHCC
>Mature Secondary Structure
MITVAKDNSGDFNSIQQAVDSIPAGTPETIYIKKGIYKERVEVRKNNISFVGESTDDTII
CEEEEECCCCCHHHHHHHHHHCCCCCCCEEEEECCCHHHHHHHCCCCEEEEECCCCCEEE
TESYYARMIMPDGSKRGTFRSYTFFVYADNFTASNLTFENAAGFGDEFGQAIAVYAEGDN
ECCEEEEEEECCCCCCCCEEEEEEEEEECCCEECCEEECCCCCCCCCCCCEEEEEECCCC
ITFRNCKILGHQDTLFTGPLPMKEKQPGGFTGPTIDGIRRVVHQLYEDCYIAGEIDFIFG
EEEEEEEEECCCCCEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCEEEEEEEEEEEE
SATAYFKNCTLFALNRNQEINAFYTAPSTYEGQAFGYVFESCTFTGNCPPKSVALSRPWR
CCEEEECCEEEEEECCCCCEEEEEECCCCCCCCEEEEEEECCEECCCCCCCCEECCCCEE
IHAKTVLLNCSYSDQIIDEGFTDWNKPESHETVYYAEYNGHGEGFKPEKRAAYVHQLNES
EEEEEEEEECCCCHHHHHCCCCCCCCCCCCCEEEEEEECCCCCCCCCHHHHHHHHHCCCC
EAALYTLENVMNS
CHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8370537 [H]