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The map label for this gene is rne [H]

Identifier: 238916950

GI number: 238916950

Start: 1032148

End: 1033377

Strand: Reverse

Name: rne [H]

Synonym: EUBELI_01019

Alternate gene names: 238916950

Gene position: 1033377-1032148 (Counterclockwise)

Preceding gene: 238916951

Following gene: 238916949

Centisome position: 48.19

GC content: 34.31

Gene sequence:

>1230_bases
ATGGAATATAAATATATAATCACAGATATTAACAATAAAATCTTCTGTTGCATATATACTTCTGATAATACCTGCCACTA
TCTTAAGCCTTTAGATAGTGGCAGTGTTGTTGGTAACATATATGTTGGAAGAGTTGAAAATGTAGTTAAAAATATTAATG
CTGCATTTATTGAGATAGAAGATAAGCAGAAATGTTATTACTCTATACAAGACAACAAAGCACCTATATTTTTTAATGAG
AAAAACAATCCTGCAATATGTCAGGGCGACAGAATTCTCGTAAAGGTCACAACCGAACCACTCAAAACCAAGCCTGCAAA
GGTGTCTTCAAAGATAGAGCTTACCGGTAATTATGCAGTTGTTTCTAACGATGTGAAAGGTGTTCATATTTCAAAAAAGA
TAAAGTCAAATGAAACAATCTCAGAGATAAAAAAACGTGTTGCTGACATTCTCATTCAGAAGCAGCGGGAAGCCATAGAA
AAAACAGGTTATGATTATCTTTCAAATTTCGGAATAATATTAAGAAGCGGCTGTGCTGACGCTGATACTGATGATATTAT
TAAAGATGTAAAAGCATTGTGTAACGAATACACTGATATGCTCCGAAAAGCTGTTTTTTCAAAGTTCTATACACTTGTAC
ATAAAGACAGACCTGAATATATTGAGGAAATTGTTCACCTGTCAGGAAAAGACCGTGTTGAGGTTATCACAGATATTCCG
GATATATATAATGAATTAGAAACATATCTGCCTCGTAGCAGCAATATGAGTATCCGTATGTATAAAGATGAATTATGGCC
TTTATACAAGCTCTATTCTATAGAAAAAGAGATAGATACCGCTCTTTCCAAGAAGGTCTGGTTAAAATCAGGTGGATATC
TTATTATCGAACAGACTGAAGCACTTTCTGTCATTGATGTTAATTCTGGTAAGAATGTAACTAAGGCAAAGAGCATGGAA
GCAATCGAGGCTTCTGCATTAAAAACTAATCTTGAAGCCGCAGACAAGCTGTGCCAGCAGATTAAGGTGAGAAATCTCTC
AGGAATTATTATCGTTGATTTTATTAATATGAATCAGAGAAATTGCGATATTCTGATGGAACATTTGAAATTTCTGGCAA
AAAAGGATATAGTAAACACTACAATCGTTGACATCACCAAGCTTGGTCTTGTTGAGATTACACGAAAGAAAATCGGAAAA
AGTCTGTCTGAAAGTCTTAATAAAGCCTGA

Upstream 100 bases:

>100_bases
TGATACTATTCTTGCAAAGGTTCGTCAGGTTGGTGCTGCATGCCACACTGGAAACAGAACATGCTTCTATAGAAATATTA
AAACATGGAACAGATAATTT

Downstream 100 bases:

>100_bases
AAATGCATGGAGGATTTATGGATAATACTGAAGTAAATATTTCACAGGAAATTCCTGCATACGAAAGAAGATATAGAAAG
ATATCAGAATGGTTTCTTAA

Product: ribonuclease G

Products: NA

Alternate protein names: RNase E [H]

Number of amino acids: Translated: 409; Mature: 409

Protein sequence:

>409_residues
MEYKYIITDINNKIFCCIYTSDNTCHYLKPLDSGSVVGNIYVGRVENVVKNINAAFIEIEDKQKCYYSIQDNKAPIFFNE
KNNPAICQGDRILVKVTTEPLKTKPAKVSSKIELTGNYAVVSNDVKGVHISKKIKSNETISEIKKRVADILIQKQREAIE
KTGYDYLSNFGIILRSGCADADTDDIIKDVKALCNEYTDMLRKAVFSKFYTLVHKDRPEYIEEIVHLSGKDRVEVITDIP
DIYNELETYLPRSSNMSIRMYKDELWPLYKLYSIEKEIDTALSKKVWLKSGGYLIIEQTEALSVIDVNSGKNVTKAKSME
AIEASALKTNLEAADKLCQQIKVRNLSGIIIVDFINMNQRNCDILMEHLKFLAKKDIVNTTIVDITKLGLVEITRKKIGK
SLSESLNKA

Sequences:

>Translated_409_residues
MEYKYIITDINNKIFCCIYTSDNTCHYLKPLDSGSVVGNIYVGRVENVVKNINAAFIEIEDKQKCYYSIQDNKAPIFFNE
KNNPAICQGDRILVKVTTEPLKTKPAKVSSKIELTGNYAVVSNDVKGVHISKKIKSNETISEIKKRVADILIQKQREAIE
KTGYDYLSNFGIILRSGCADADTDDIIKDVKALCNEYTDMLRKAVFSKFYTLVHKDRPEYIEEIVHLSGKDRVEVITDIP
DIYNELETYLPRSSNMSIRMYKDELWPLYKLYSIEKEIDTALSKKVWLKSGGYLIIEQTEALSVIDVNSGKNVTKAKSME
AIEASALKTNLEAADKLCQQIKVRNLSGIIIVDFINMNQRNCDILMEHLKFLAKKDIVNTTIVDITKLGLVEITRKKIGK
SLSESLNKA
>Mature_409_residues
MEYKYIITDINNKIFCCIYTSDNTCHYLKPLDSGSVVGNIYVGRVENVVKNINAAFIEIEDKQKCYYSIQDNKAPIFFNE
KNNPAICQGDRILVKVTTEPLKTKPAKVSSKIELTGNYAVVSNDVKGVHISKKIKSNETISEIKKRVADILIQKQREAIE
KTGYDYLSNFGIILRSGCADADTDDIIKDVKALCNEYTDMLRKAVFSKFYTLVHKDRPEYIEEIVHLSGKDRVEVITDIP
DIYNELETYLPRSSNMSIRMYKDELWPLYKLYSIEKEIDTALSKKVWLKSGGYLIIEQTEALSVIDVNSGKNVTKAKSME
AIEASALKTNLEAADKLCQQIKVRNLSGIIIVDFINMNQRNCDILMEHLKFLAKKDIVNTTIVDITKLGLVEITRKKIGK
SLSESLNKA

Specific function: Matures 5s Rrna From Its Precursors From All The Rrna Genes. It Also Cleaves RNA I, A Molecule That Controls The Replication Of Cole1 Plasmid DNA. It Is The Major Endoribonuclease Participating In Mrna Turnover In E. coli. It Initiates The Decay Of Rnas B

COG id: COG1530

COG function: function code J; Ribonucleases G and E

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain [H]

Homologues:

Organism=Escherichia coli, GI1787325, Length=382, Percent_Identity=25.9162303664921, Blast_Score=135, Evalue=3e-33,
Organism=Escherichia coli, GI87082235, Length=398, Percent_Identity=29.1457286432161, Blast_Score=131, Evalue=7e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR003029
- InterPro:   IPR019307
- InterPro:   IPR022967
- InterPro:   IPR004659 [H]

Pfam domain/function: PF10150 RNase_E_G; PF00575 S1 [H]

EC number: =3.1.26.12 [H]

Molecular weight: Translated: 46429; Mature: 46429

Theoretical pI: Translated: 8.27; Mature: 8.27

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEYKYIITDINNKIFCCIYTSDNTCHYLKPLDSGSVVGNIYVGRVENVVKNINAAFIEIE
CCEEEEEEECCCEEEEEEEECCCCEEEECCCCCCCEEEEEEHHHHHHHHHCCCEEEEEEE
DKQKCYYSIQDNKAPIFFNEKNNPAICQGDRILVKVTTEPLKTKPAKVSSKIELTGNYAV
CCCCEEEEEECCCCCEEEECCCCCCEECCCEEEEEEECCCCCCCCCCCCCEEEEECCEEE
VSNDVKGVHISKKIKSNETISEIKKRVADILIQKQREAIEKTGYDYLSNFGIILRSGCAD
EECCCCCEEEEHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCEEECCCCC
ADTDDIIKDVKALCNEYTDMLRKAVFSKFYTLVHKDRPEYIEEIVHLSGKDRVEVITDIP
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCEEEECCCH
DIYNELETYLPRSSNMSIRMYKDELWPLYKLYSIEKEIDTALSKKVWLKSGGYLIIEQTE
HHHHHHHHHCCCCCCCEEEEEHHHCCHHHHHHHHHHHHHHHHHHHHEEECCCEEEEEECC
ALSVIDVNSGKNVTKAKSMEAIEASALKTNLEAADKLCQQIKVRNLSGIIIVDFINMNQR
CEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCC
NCDILMEHLKFLAKKDIVNTTIVDITKLGLVEITRKKIGKSLSESLNKA
CHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MEYKYIITDINNKIFCCIYTSDNTCHYLKPLDSGSVVGNIYVGRVENVVKNINAAFIEIE
CCEEEEEEECCCEEEEEEEECCCCEEEECCCCCCCEEEEEEHHHHHHHHHCCCEEEEEEE
DKQKCYYSIQDNKAPIFFNEKNNPAICQGDRILVKVTTEPLKTKPAKVSSKIELTGNYAV
CCCCEEEEEECCCCCEEEECCCCCCEECCCEEEEEEECCCCCCCCCCCCCEEEEECCEEE
VSNDVKGVHISKKIKSNETISEIKKRVADILIQKQREAIEKTGYDYLSNFGIILRSGCAD
EECCCCCEEEEHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCEEECCCCC
ADTDDIIKDVKALCNEYTDMLRKAVFSKFYTLVHKDRPEYIEEIVHLSGKDRVEVITDIP
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCEEEECCCH
DIYNELETYLPRSSNMSIRMYKDELWPLYKLYSIEKEIDTALSKKVWLKSGGYLIIEQTE
HHHHHHHHHCCCCCCCEEEEEHHHCCHHHHHHHHHHHHHHHHHHHHEEECCCEEEEEECC
ALSVIDVNSGKNVTKAKSMEAIEASALKTNLEAADKLCQQIKVRNLSGIIIVDFINMNQR
CEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCC
NCDILMEHLKFLAKKDIVNTTIVDITKLGLVEITRKKIGKSLSESLNKA
CHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]