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The map label for this gene is miaA [H]

Identifier: 238916923

GI number: 238916923

Start: 1011020

End: 1011961

Strand: Direct

Name: miaA [H]

Synonym: EUBELI_00992

Alternate gene names: 238916923

Gene position: 1011020-1011961 (Clockwise)

Preceding gene: 238916922

Following gene: 238916924

Centisome position: 47.15

GC content: 38.54

Gene sequence:

>942_bases
ATGAAAAAAGAACCTTTAATAATACTTACAGGTCCGACAGCAGTTGGAAAGACAGAGCTTTCCATAGAACTTGCAAAGGC
GGTAAATGGTGAGATAATAAGTGCCGATTCCATGCAGGTATACAGATATATGGATATAGGAACTGCCAAGATAACAAAAG
AAGAGATGCAGGGGGTTAAGCATTATCTTGTTGATGAGATTGAGCCTTCAGAGGGCTTTGATGTGGCAAGATTTAAGTCT
TTGGCAACAAATGCAATAGAAAAAATCCGTGAGAACGGTCATATTCCTGTAGTTGTTGGCGGAACCGGATTTTATATACA
GGCACTTTTATATGATTCTACATTTGAAGATGATGAAAGTGACTATGAATATAGGGATTATCTTCAGGGGATTGCTGATA
CTAAGGGTGCAGAGGTGCTTCATGATATGTTAAAGGTAGCTGACCCTGAATCTGCAGAGGCAATTCATGCTAATAATGTC
AAAAGAGTTATTCGTGCACTTGAATTTTATAAGACGACAGGCAGAAAGATATCAGAGCATAATAATGAGCAGAGAGCTAA
AGAGTCGCCTTATAATTTTTGTTACTTTGTTCTTAATGATAAAAGAGAAATTTTATATGACAGGATAAACAGGCGTGTTG
ATATAATGTTTGACAACGGACTGCTTGATGAGGTAAAACATTTGTCGGACATGGGGCTTGTAAAGGACGATGTTGCCATG
CAGGGAATCGGATATAAAGAAGTGTTCGATTATCTTGAGGGCAGATGTGACGAGGCAGGTCTTAGAGAGATGATTAAGCA
GGATACAAGACATTTTGCAAAAAGACAGCTTACATGGTTTCGGAGAGAGCCGTTGGTTACATGGGTGGATTTTTCCCAGA
TACGAAGAGAAGATGCGCTTTCGTATATGCTTGAACAACTAAAAGAAAAGGAAATTATTTAA

Upstream 100 bases:

>100_bases
CAGAGAATCCATATAACTGTCCGCATGGCAGACCAACACTAATTATGATGAGCAGATATGAGATAGAAAAGAAATTTAAA
CGAATAGTATAGAGGCTGAT

Downstream 100 bases:

>100_bases
TTATGAGAGAAATGTATAAGAATATGGGAATCTGTGATGAAGTATATGATTATTGCGACAATATAATTAAGTCGCTTCAT
GACAGATTTGAAGAGATAGA

Product: tRNA delta(2)-isopentenylpyrophosphate transferase

Products: NA

Alternate protein names: Dimethylallyl diphosphate:tRNA dimethylallyltransferase; DMAPP:tRNA dimethylallyltransferase; DMATase; Isopentenyl-diphosphate:tRNA isopentenyltransferase; IPP transferase; IPPT; IPTase [H]

Number of amino acids: Translated: 313; Mature: 313

Protein sequence:

>313_residues
MKKEPLIILTGPTAVGKTELSIELAKAVNGEIISADSMQVYRYMDIGTAKITKEEMQGVKHYLVDEIEPSEGFDVARFKS
LATNAIEKIRENGHIPVVVGGTGFYIQALLYDSTFEDDESDYEYRDYLQGIADTKGAEVLHDMLKVADPESAEAIHANNV
KRVIRALEFYKTTGRKISEHNNEQRAKESPYNFCYFVLNDKREILYDRINRRVDIMFDNGLLDEVKHLSDMGLVKDDVAM
QGIGYKEVFDYLEGRCDEAGLREMIKQDTRHFAKRQLTWFRREPLVTWVDFSQIRREDALSYMLEQLKEKEII

Sequences:

>Translated_313_residues
MKKEPLIILTGPTAVGKTELSIELAKAVNGEIISADSMQVYRYMDIGTAKITKEEMQGVKHYLVDEIEPSEGFDVARFKS
LATNAIEKIRENGHIPVVVGGTGFYIQALLYDSTFEDDESDYEYRDYLQGIADTKGAEVLHDMLKVADPESAEAIHANNV
KRVIRALEFYKTTGRKISEHNNEQRAKESPYNFCYFVLNDKREILYDRINRRVDIMFDNGLLDEVKHLSDMGLVKDDVAM
QGIGYKEVFDYLEGRCDEAGLREMIKQDTRHFAKRQLTWFRREPLVTWVDFSQIRREDALSYMLEQLKEKEII
>Mature_313_residues
MKKEPLIILTGPTAVGKTELSIELAKAVNGEIISADSMQVYRYMDIGTAKITKEEMQGVKHYLVDEIEPSEGFDVARFKS
LATNAIEKIRENGHIPVVVGGTGFYIQALLYDSTFEDDESDYEYRDYLQGIADTKGAEVLHDMLKVADPESAEAIHANNV
KRVIRALEFYKTTGRKISEHNNEQRAKESPYNFCYFVLNDKREILYDRINRRVDIMFDNGLLDEVKHLSDMGLVKDDVAM
QGIGYKEVFDYLEGRCDEAGLREMIKQDTRHFAKRQLTWFRREPLVTWVDFSQIRREDALSYMLEQLKEKEII

Specific function: Catalyzes the transfer of a dimethylallyl group onto the adenine at position 37 in tRNAs that read codons beginning with uridine, leading to the formation of N6-(dimethylallyl)adenosine (i(6)A) [H]

COG id: COG0324

COG function: function code J; tRNA delta(2)-isopentenylpyrophosphate transferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IPP transferase family [H]

Homologues:

Organism=Homo sapiens, GI31581534, Length=308, Percent_Identity=30.8441558441558, Blast_Score=145, Evalue=5e-35,
Organism=Escherichia coli, GI1790613, Length=289, Percent_Identity=37.3702422145329, Blast_Score=174, Evalue=1e-44,
Organism=Caenorhabditis elegans, GI25144712, Length=279, Percent_Identity=34.4086021505376, Blast_Score=139, Evalue=2e-33,
Organism=Saccharomyces cerevisiae, GI6324848, Length=289, Percent_Identity=35.2941176470588, Blast_Score=175, Evalue=8e-45,
Organism=Drosophila melanogaster, GI24649830, Length=270, Percent_Identity=37.7777777777778, Blast_Score=176, Evalue=2e-44,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002627
- InterPro:   IPR018022 [H]

Pfam domain/function: PF01715 IPPT [H]

EC number: =2.5.1.75 [H]

Molecular weight: Translated: 36131; Mature: 36131

Theoretical pI: Translated: 4.87; Mature: 4.87

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKEPLIILTGPTAVGKTELSIELAKAVNGEIISADSMQVYRYMDIGTAKITKEEMQGVK
CCCCCEEEEECCCCCCCCHHHHHHHHHCCCCEEECCCCEEEHEEECCHHHHHHHHHHHHH
HYLVDEIEPSEGFDVARFKSLATNAIEKIRENGHIPVVVGGTGFYIQALLYDSTFEDDES
HHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHCCCCCCCCC
DYEYRDYLQGIADTKGAEVLHDMLKVADPESAEAIHANNVKRVIRALEFYKTTGRKISEH
CCHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHC
NNEQRAKESPYNFCYFVLNDKREILYDRINRRVDIMFDNGLLDEVKHLSDMGLVKDDVAM
CCHHHHHCCCCCEEEEEECCCHHHHHHHHCCEEEEEECCCHHHHHHHHHHCCCHHHHHHH
QGIGYKEVFDYLEGRCDEAGLREMIKQDTRHFAKRQLTWFRREPLVTWVDFSQIRREDAL
CCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECHHHHHHHHHH
SYMLEQLKEKEII
HHHHHHHHHCCCC
>Mature Secondary Structure
MKKEPLIILTGPTAVGKTELSIELAKAVNGEIISADSMQVYRYMDIGTAKITKEEMQGVK
CCCCCEEEEECCCCCCCCHHHHHHHHHCCCCEEECCCCEEEHEEECCHHHHHHHHHHHHH
HYLVDEIEPSEGFDVARFKSLATNAIEKIRENGHIPVVVGGTGFYIQALLYDSTFEDDES
HHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHCCCCCCCCC
DYEYRDYLQGIADTKGAEVLHDMLKVADPESAEAIHANNVKRVIRALEFYKTTGRKISEH
CCHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHC
NNEQRAKESPYNFCYFVLNDKREILYDRINRRVDIMFDNGLLDEVKHLSDMGLVKDDVAM
CCHHHHHCCCCCEEEEEECCCHHHHHHHHCCEEEEEECCCHHHHHHHHHHCCCHHHHHHH
QGIGYKEVFDYLEGRCDEAGLREMIKQDTRHFAKRQLTWFRREPLVTWVDFSQIRREDAL
CCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECHHHHHHHHHH
SYMLEQLKEKEII
HHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA