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The map label for this gene is yfiF [H]

Identifier: 238916910

GI number: 238916910

Start: 993768

End: 994670

Strand: Direct

Name: yfiF [H]

Synonym: EUBELI_00979

Alternate gene names: 238916910

Gene position: 993768-994670 (Clockwise)

Preceding gene: 238916908

Following gene: 238916911

Centisome position: 46.35

GC content: 35.88

Gene sequence:

>903_bases
ATGTTTCTATACAAGTTGGAGGTGTCAGATATGGGGAATGTAGTTGATAAAGGAACCAGCTTTTTGTTTCATGTGAACAG
GTATGTGGCAGGTAGTGAAACTGACATGTATAAACATGGATATGCGGAGCTTTTATATGTGTCTGATGGAAGCCTTACTG
AGCATGTATGTGGGGATAAGGTGCATATAAAGAAAGGCGATGTATGCTTTATTGATTCGGGGTGTGTACATAAGGAAAGC
TTTGAGGAAGATGACACATTTGTAATAAGACTCGAAGTGTCAGATGAGATTATTAATGCAGTTATAAGTAATGCTATGGC
GGATAAACAGGCGGTGGATTATATTAAAGAGCTTGTTAAAGTGTTAAAGAACAGCGGATATATACATTTTGGCTATAAAG
ATGATTATGATAATGAGCTTAAGGATATGCTTACTGCTATGATAAGCGAGTGTGGTGTGGCGGATATGGCGTCTGCATAT
ATATGTCAGGGGCTTATGCTTAGGATTCTGTGGCGGCTAGGAACAGTATATGAGTATGCACAGTCAAGATACATAAGAAA
GAAAATTAACTGGCTTTTGCATCAGGAAATTACAGATTTTATTGAAGATAATATGAAAGATGTTACAATAGATATGCTTG
TTGAAAGATTCGGTTATCAGGAGGATTATTTCAACAGGTTTTTAAAGGCACAGACAGGTATGACTTTCACGGAATATCTG
CAGGATTGCAGATTGAACAAATCGGCCATTCTCTTAAAAGAAGATTCAATGGATGTTGATGAGATTATAAAGAAAGTCGG
ATATAGAAACAAAGGATATTTTTACAGGATATTTACTGAGAAATACAACATGACACCTGCAAAATTCAGAAAATATATTA
AAGAAAAGGAGTTTACGATATGA

Upstream 100 bases:

>100_bases
TATTACAAATGTTTTATCGAAAGAGAGTGATAATACGCCAAAAGTTACCTGCAACGACTTTTTGAAATAAAAGTTTCCTA
CAACGACTTTTTGTGATAGA

Downstream 100 bases:

>100_bases
GTGATATGTATAACAATCCGGTACAGAGAGGATTCTTTCCAGATCCTTCGGTAGTAAGAGTTGGAGATGACTATTATATG
GCTAATTCAACATTTCAGTA

Product: 2-isopropylmalate synthase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 300; Mature: 300

Protein sequence:

>300_residues
MFLYKLEVSDMGNVVDKGTSFLFHVNRYVAGSETDMYKHGYAELLYVSDGSLTEHVCGDKVHIKKGDVCFIDSGCVHKES
FEEDDTFVIRLEVSDEIINAVISNAMADKQAVDYIKELVKVLKNSGYIHFGYKDDYDNELKDMLTAMISECGVADMASAY
ICQGLMLRILWRLGTVYEYAQSRYIRKKINWLLHQEITDFIEDNMKDVTIDMLVERFGYQEDYFNRFLKAQTGMTFTEYL
QDCRLNKSAILLKEDSMDVDEIIKKVGYRNKGYFYRIFTEKYNMTPAKFRKYIKEKEFTI

Sequences:

>Translated_300_residues
MFLYKLEVSDMGNVVDKGTSFLFHVNRYVAGSETDMYKHGYAELLYVSDGSLTEHVCGDKVHIKKGDVCFIDSGCVHKES
FEEDDTFVIRLEVSDEIINAVISNAMADKQAVDYIKELVKVLKNSGYIHFGYKDDYDNELKDMLTAMISECGVADMASAY
ICQGLMLRILWRLGTVYEYAQSRYIRKKINWLLHQEITDFIEDNMKDVTIDMLVERFGYQEDYFNRFLKAQTGMTFTEYL
QDCRLNKSAILLKEDSMDVDEIIKKVGYRNKGYFYRIFTEKYNMTPAKFRKYIKEKEFTI
>Mature_300_residues
MFLYKLEVSDMGNVVDKGTSFLFHVNRYVAGSETDMYKHGYAELLYVSDGSLTEHVCGDKVHIKKGDVCFIDSGCVHKES
FEEDDTFVIRLEVSDEIINAVISNAMADKQAVDYIKELVKVLKNSGYIHFGYKDDYDNELKDMLTAMISECGVADMASAY
ICQGLMLRILWRLGTVYEYAQSRYIRKKINWLLHQEITDFIEDNMKDVTIDMLVERFGYQEDYFNRFLKAQTGMTFTEYL
QDCRLNKSAILLKEDSMDVDEIIKKVGYRNKGYFYRIFTEKYNMTPAKFRKYIKEKEFTI

Specific function: Positive Activator Of Genes Required For L-Rhamnose Utilization, Probably Of The Rhamnose Transporter Gene (Rhat). [C]

COG id: COG2207

COG function: function code K; AraC-type DNA-binding domain-containing proteins

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH araC/xylS-type DNA-binding domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009057
- InterPro:   IPR012287
- InterPro:   IPR003313
- InterPro:   IPR018062
- InterPro:   IPR018060 [H]

Pfam domain/function: PF02311 AraC_binding; PF00165 HTH_AraC [H]

EC number: NA

Molecular weight: Translated: 35077; Mature: 35077

Theoretical pI: Translated: 5.15; Mature: 5.15

Prosite motif: PS01124 HTH_ARAC_FAMILY_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
6.3 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
6.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFLYKLEVSDMGNVVDKGTSFLFHVNRYVAGSETDMYKHGYAELLYVSDGSLTEHVCGDK
CEEEEEEHHHHCHHHHHCCHHEEEHHHHHCCCCCHHHHCCCEEEEEEECCCHHHHHCCCE
VHIKKGDVCFIDSGCVHKESFEEDDTFVIRLEVSDEIINAVISNAMADKQAVDYIKELVK
EEEECCCEEEECCCCCCCCCCCCCCEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHH
VLKNSGYIHFGYKDDYDNELKDMLTAMISECGVADMASAYICQGLMLRILWRLGTVYEYA
HHHCCCEEEECCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QSRYIRKKINWLLHQEITDFIEDNMKDVTIDMLVERFGYQEDYFNRFLKAQTGMTFTEYL
HHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCHHHHH
QDCRLNKSAILLKEDSMDVDEIIKKVGYRNKGYFYRIFTEKYNMTPAKFRKYIKEKEFTI
HHHCCCCCEEEEECCCCCHHHHHHHHCCCCCCEEEEEEECCCCCCHHHHHHHHHHCCCCC
>Mature Secondary Structure
MFLYKLEVSDMGNVVDKGTSFLFHVNRYVAGSETDMYKHGYAELLYVSDGSLTEHVCGDK
CEEEEEEHHHHCHHHHHCCHHEEEHHHHHCCCCCHHHHCCCEEEEEEECCCHHHHHCCCE
VHIKKGDVCFIDSGCVHKESFEEDDTFVIRLEVSDEIINAVISNAMADKQAVDYIKELVK
EEEECCCEEEECCCCCCCCCCCCCCEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHH
VLKNSGYIHFGYKDDYDNELKDMLTAMISECGVADMASAYICQGLMLRILWRLGTVYEYA
HHHCCCEEEECCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QSRYIRKKINWLLHQEITDFIEDNMKDVTIDMLVERFGYQEDYFNRFLKAQTGMTFTEYL
HHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCHHHHH
QDCRLNKSAILLKEDSMDVDEIIKKVGYRNKGYFYRIFTEKYNMTPAKFRKYIKEKEFTI
HHHCCCCCEEEEECCCCCHHHHHHHHCCCCCCEEEEEEECCCCCCHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8704981; 9384377 [H]