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The map label for this gene is nusA [H]

Identifier: 238916823

GI number: 238916823

Start: 902037

End: 903182

Strand: Direct

Name: nusA [H]

Synonym: EUBELI_00891

Alternate gene names: 238916823

Gene position: 902037-903182 (Clockwise)

Preceding gene: 238916822

Following gene: 238916824

Centisome position: 42.07

GC content: 38.66

Gene sequence:

>1146_bases
ATGAATAAGGAATTAATTATGGCACTTGATGCTCTTGAGAAGGAGAATGGAATCGATAAAGAGATTATGTTTGCAGCTAT
CGAGAAATCTCTTATGGATGAGTACAAGGCTGAGTTTGATAAGGCTGATAACGGTCGTGTTGAGTTAGACAGAAGAACAG
GTGATTTCCACATTTATTCTGACAGAACTGTTGTTGAGGAAGTTATTGTTCCAGAGAACAGAGAGAATAAGAAAGAAAAA
TATGTATCAGGTACTGATATTGCATTAGAAGATGCCAGAAAGATTAAGCCTGACTGCCAGCTTGGTGATGTTATTACTGT
TGAAGTAAAGTCTGAGGAGTTCTCAAGAAAGGCTGCCAAGAATGCTAAGAATACTATTGTGCAGACTATCAGAGAGCAGG
AAAAGAATGCTCTTTATAATGAGTATCATTCTAAGGAGAAGGAACTTATTACAGGTATTGTGCAGAGAGTTGCTGACAAT
GGTGATCTTACAATTGATCTTGGAAGACTCCAGACAGTTCTTAAGGCTGATGACAAGTTCAAGGATAAGAAGTTTGTGCC
AGGCGATAGAATTAAGTTATATGTTGTTGATGTAATTAACAGAGAAAAGGGTGGTCCTGTTGTAAGAGTTTCAAGAAAGT
CACAGGAGCTTGTTAAGAAACTTTTTGAAGAAGAAGTTACAGAGATTAAAGATGGCGTTGTTGAAATCATGGGTATTGCA
AGAGAAGCCGGCTCAAGAACTAAGATGGCAGTAAGAGCCAATGTTGCAAATGTAGATCCTGTAGGTGCATGTGTCGGTAT
TAACGGTGCAAGAGTTAAAGCTATTGTTAATGAACTTGGCAATGAGCAGATAGATATCATTGAGTGGGACAGCAATTCAG
CACAGCTTATCGTTAACGCATTAAGCCCTGCAAAGGTTGTTTCAGCAGTAGCTGATGATGAAGAGAAGAAGGCTAAGATT
GTTGTTTCTGAGCAGCAGCTTTCTTTAGCAATTGGTAAGCAGGGACAGAATGTAAGACTTGCTGCAAAGCTTACAGGATA
TGGAATTGATATTAAGAGCGAGGCTGAACCTGAAGAACATACAGAAGAAGAGAACTTAGAAGAAGAGTATGTTGAACCAT
CTGAATTAAGCCTTGATGAAGAATAA

Upstream 100 bases:

>100_bases
TTGATGATGTAACTAAAACATTTGACAGAAGTAATCTGGCTATGATCAGATGGGCATTTGTAGATGAAGTATAATATATT
TTTGGAGGAAATTAAAAGCA

Downstream 100 bases:

>100_bases
TTATTAGGAGTGATGCATAGTGGCTACAGTAAAGAAGATTCCACAAAGACAGTGTATTGGCTGCGGTCAGATGAAGGATA
AGAAGGACTTAATCCGTATT

Product: N utilization substance protein A

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 381; Mature: 381

Protein sequence:

>381_residues
MNKELIMALDALEKENGIDKEIMFAAIEKSLMDEYKAEFDKADNGRVELDRRTGDFHIYSDRTVVEEVIVPENRENKKEK
YVSGTDIALEDARKIKPDCQLGDVITVEVKSEEFSRKAAKNAKNTIVQTIREQEKNALYNEYHSKEKELITGIVQRVADN
GDLTIDLGRLQTVLKADDKFKDKKFVPGDRIKLYVVDVINREKGGPVVRVSRKSQELVKKLFEEEVTEIKDGVVEIMGIA
REAGSRTKMAVRANVANVDPVGACVGINGARVKAIVNELGNEQIDIIEWDSNSAQLIVNALSPAKVVSAVADDEEKKAKI
VVSEQQLSLAIGKQGQNVRLAAKLTGYGIDIKSEAEPEEHTEEENLEEEYVEPSELSLDEE

Sequences:

>Translated_381_residues
MNKELIMALDALEKENGIDKEIMFAAIEKSLMDEYKAEFDKADNGRVELDRRTGDFHIYSDRTVVEEVIVPENRENKKEK
YVSGTDIALEDARKIKPDCQLGDVITVEVKSEEFSRKAAKNAKNTIVQTIREQEKNALYNEYHSKEKELITGIVQRVADN
GDLTIDLGRLQTVLKADDKFKDKKFVPGDRIKLYVVDVINREKGGPVVRVSRKSQELVKKLFEEEVTEIKDGVVEIMGIA
REAGSRTKMAVRANVANVDPVGACVGINGARVKAIVNELGNEQIDIIEWDSNSAQLIVNALSPAKVVSAVADDEEKKAKI
VVSEQQLSLAIGKQGQNVRLAAKLTGYGIDIKSEAEPEEHTEEENLEEEYVEPSELSLDEE
>Mature_381_residues
MNKELIMALDALEKENGIDKEIMFAAIEKSLMDEYKAEFDKADNGRVELDRRTGDFHIYSDRTVVEEVIVPENRENKKEK
YVSGTDIALEDARKIKPDCQLGDVITVEVKSEEFSRKAAKNAKNTIVQTIREQEKNALYNEYHSKEKELITGIVQRVADN
GDLTIDLGRLQTVLKADDKFKDKKFVPGDRIKLYVVDVINREKGGPVVRVSRKSQELVKKLFEEEVTEIKDGVVEIMGIA
REAGSRTKMAVRANVANVDPVGACVGINGARVKAIVNELGNEQIDIIEWDSNSAQLIVNALSPAKVVSAVADDEEKKAKI
VVSEQQLSLAIGKQGQNVRLAAKLTGYGIDIKSEAEPEEHTEEENLEEEYVEPSELSLDEE

Specific function: Participates in both the termination and antitermination of transcription. Interacts with RNA polymerase and binds RNA [H]

COG id: COG0195

COG function: function code K; Transcription elongation factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain [H]

Homologues:

Organism=Escherichia coli, GI1789560, Length=369, Percent_Identity=37.1273712737127, Blast_Score=238, Evalue=6e-64,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1612 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004087
- InterPro:   IPR015946
- InterPro:   IPR009019
- InterPro:   IPR004088
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR003029
- InterPro:   IPR022967
- InterPro:   IPR013735
- InterPro:   IPR010213 [H]

Pfam domain/function: PF08529 NusA_N; PF00575 S1 [H]

EC number: NA

Molecular weight: Translated: 42560; Mature: 42560

Theoretical pI: Translated: 4.56; Mature: 4.56

Prosite motif: PS50084 KH_TYPE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKELIMALDALEKENGIDKEIMFAAIEKSLMDEYKAEFDKADNGRVELDRRTGDFHIYS
CCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCEEEEC
DRTVVEEVIVPENRENKKEKYVSGTDIALEDARKIKPDCQLGDVITVEVKSEEFSRKAAK
CCHHHHHHHCCCCCCCHHHHHCCCCCEEHHHHHHCCCCCCCCCEEEEEECCHHHHHHHHH
NAKNTIVQTIREQEKNALYNEYHSKEKELITGIVQRVADNGDLTIDLGRLQTVLKADDKF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHCCCCC
KDKKFVPGDRIKLYVVDVINREKGGPVVRVSRKSQELVKKLFEEEVTEIKDGVVEIMGIA
CCCCCCCCCCEEEEEEEEECCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
REAGSRTKMAVRANVANVDPVGACVGINGARVKAIVNELGNEQIDIIEWDSNSAQLIVNA
HHCCCCCEEEEEECCCCCCCCCEEECCCCHHHHHHHHHHCCCEEEEEEECCCCCEEEEEC
LSPAKVVSAVADDEEKKAKIVVSEQQLSLAIGKQGQNVRLAAKLTGYGIDIKSEAEPEEH
CCHHHHHHHHHCCCCCCEEEEEEHHHHHHEECCCCCCEEEEEEEECCCEEECCCCCCHHC
TEEENLEEEYVEPSELSLDEE
CHHHCCHHHCCCCCCCCCCCC
>Mature Secondary Structure
MNKELIMALDALEKENGIDKEIMFAAIEKSLMDEYKAEFDKADNGRVELDRRTGDFHIYS
CCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCEEEEC
DRTVVEEVIVPENRENKKEKYVSGTDIALEDARKIKPDCQLGDVITVEVKSEEFSRKAAK
CCHHHHHHHCCCCCCCHHHHHCCCCCEEHHHHHHCCCCCCCCCEEEEEECCHHHHHHHHH
NAKNTIVQTIREQEKNALYNEYHSKEKELITGIVQRVADNGDLTIDLGRLQTVLKADDKF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHCCCCC
KDKKFVPGDRIKLYVVDVINREKGGPVVRVSRKSQELVKKLFEEEVTEIKDGVVEIMGIA
CCCCCCCCCCEEEEEEEEECCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
REAGSRTKMAVRANVANVDPVGACVGINGARVKAIVNELGNEQIDIIEWDSNSAQLIVNA
HHCCCCCEEEEEECCCCCCCCCEEECCCCHHHHHHHHHHCCCEEEEEEECCCCCEEEEEC
LSPAKVVSAVADDEEKKAKIVVSEQQLSLAIGKQGQNVRLAAKLTGYGIDIKSEAEPEEH
CCHHHHHHHHHCCCCCCEEEEEEHHHHHHEECCCCCCEEEEEEEECCCEEECCCCCCHHC
TEEENLEEEYVEPSELSLDEE
CHHHCCHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8491709; 9384377 [H]