Click here to switch to the map view.

The map label for this gene is ispG [H]

Identifier: 238916792

GI number: 238916792

Start: 865848

End: 866903

Strand: Direct

Name: ispG [H]

Synonym: EUBELI_00854

Alternate gene names: 238916792

Gene position: 865848-866903 (Clockwise)

Preceding gene: 238916791

Following gene: 238916793

Centisome position: 40.38

GC content: 39.96

Gene sequence:

>1056_bases
ATGTACAGAGATAATACTAAAGTTGTTAAGATAGGAAAATGTGTTATAGGTGGTGGCAATCCTATTCTTATCCAGTCAAT
GACTAATACACATACAGAAGATGTTGCTGCAACAGTAGCGCAGATTAAGGAGCTTACTAAAGCTGGTTGTGAAATCATCA
GATGCACAGTTCCAACTAAGGAAGCTGCACTTGCAATCAAAGAGATAAAGAAGCAGATAGAGATACCTCTCGTTGCAGAT
ATACATTTTGATTACAAGATGGCTATTATGGCAATGGAGAATGGGGCTGACAAGATTAGAATCAATCCGGGTAATATCGG
CGGAACAGACAAGATAAAGGCTGTTGTTGATGTTGCAAGGGAAAGAAATATACCAATCAGGGTTGGTGTCAACAGCGGTT
CACTTGAGAAAGACCTTATTGAGAAATATGGCGGAGTAACAGCAGAGGGAATAGTTGAGAGCGCACTTGACAAGGTTAAG
ATTATTGAAGACTTAGGTTATGATAATCTTGTTGTCAGCATCAAATCTTCTGATGTAATGATGTGTGTTAAAGCACATGA
GCTTATTGCAAAACAGACTAATCATCCGCTTCATGTTGGAATTACTGAGTCTGGAACAATATTCTCAGGAAGCATTAAGT
CATCAATCGGACTTGGCCTTATACTGAATCAGGGAATTGGTGACACAATAAGAGTTTCTCTTACAGATGATCCTGTAAGG
GAAATAGAGGCTGCGAAACTGATACTTAAGACTCTTGGCTTAAGAAAAGGTGGAATAGAGGTTGTTTCATGCCCTACATG
CGGAAGAACAAGAATTAATCTTATAGACCTTGCAGGAAAGGTTGAGAAAATGGTTTCAGGGTATGACCTTGACTTGAAGC
TTGCTGTAATGGGATGTGTAGTTAACGGACCGGGAGAAGCAAAGGAAGCTGATCTTGGAATTGCAGGCGGTGACGGCTGC
GGAGTACTTATTAAACATGGAGAGATTATTAAGAAGGTTCCAGAAGAGGAACTTCTTGATACACTCAGGTATGAACTTGA
TAATTGGAATGAGTGA

Upstream 100 bases:

>100_bases
TGTTAATTTCATAGGTATGATACTTCTTGTCATACTTATGGTTGTTGTATTGTTTAAAGATATAATGGCATTGTTCTAAT
ACAGAAAGGACACAGGGGTT

Downstream 100 bases:

>100_bases
TACATATGTACAGATTTTTTGAAGTGTTTAAGACGCTGAAGCTGCCAGAGGATCTGGCGGTGTATTTTGAGAATGTTGAA
GTAACTAAAGTCTCGAAAAC

Product: 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase

Products: NA

Alternate protein names: 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate synthase [H]

Number of amino acids: Translated: 351; Mature: 351

Protein sequence:

>351_residues
MYRDNTKVVKIGKCVIGGGNPILIQSMTNTHTEDVAATVAQIKELTKAGCEIIRCTVPTKEAALAIKEIKKQIEIPLVAD
IHFDYKMAIMAMENGADKIRINPGNIGGTDKIKAVVDVARERNIPIRVGVNSGSLEKDLIEKYGGVTAEGIVESALDKVK
IIEDLGYDNLVVSIKSSDVMMCVKAHELIAKQTNHPLHVGITESGTIFSGSIKSSIGLGLILNQGIGDTIRVSLTDDPVR
EIEAAKLILKTLGLRKGGIEVVSCPTCGRTRINLIDLAGKVEKMVSGYDLDLKLAVMGCVVNGPGEAKEADLGIAGGDGC
GVLIKHGEIIKKVPEEELLDTLRYELDNWNE

Sequences:

>Translated_351_residues
MYRDNTKVVKIGKCVIGGGNPILIQSMTNTHTEDVAATVAQIKELTKAGCEIIRCTVPTKEAALAIKEIKKQIEIPLVAD
IHFDYKMAIMAMENGADKIRINPGNIGGTDKIKAVVDVARERNIPIRVGVNSGSLEKDLIEKYGGVTAEGIVESALDKVK
IIEDLGYDNLVVSIKSSDVMMCVKAHELIAKQTNHPLHVGITESGTIFSGSIKSSIGLGLILNQGIGDTIRVSLTDDPVR
EIEAAKLILKTLGLRKGGIEVVSCPTCGRTRINLIDLAGKVEKMVSGYDLDLKLAVMGCVVNGPGEAKEADLGIAGGDGC
GVLIKHGEIIKKVPEEELLDTLRYELDNWNE
>Mature_351_residues
MYRDNTKVVKIGKCVIGGGNPILIQSMTNTHTEDVAATVAQIKELTKAGCEIIRCTVPTKEAALAIKEIKKQIEIPLVAD
IHFDYKMAIMAMENGADKIRINPGNIGGTDKIKAVVDVARERNIPIRVGVNSGSLEKDLIEKYGGVTAEGIVESALDKVK
IIEDLGYDNLVVSIKSSDVMMCVKAHELIAKQTNHPLHVGITESGTIFSGSIKSSIGLGLILNQGIGDTIRVSLTDDPVR
EIEAAKLILKTLGLRKGGIEVVSCPTCGRTRINLIDLAGKVEKMVSGYDLDLKLAVMGCVVNGPGEAKEADLGIAGGDGC
GVLIKHGEIIKKVPEEELLDTLRYELDNWNE

Specific function: Converts 2C-methyl-D-erythritol 2,4-cyclodiphosphate (ME-2,4cPP) into 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate [H]

COG id: COG0821

COG function: function code I; Enzyme involved in the deoxyxylulose pathway of isoprenoid biosynthesis

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispG family [H]

Homologues:

Organism=Escherichia coli, GI1788863, Length=342, Percent_Identity=48.2456140350877, Blast_Score=335, Evalue=3e-93,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR011005
- InterPro:   IPR016425
- InterPro:   IPR004588 [H]

Pfam domain/function: PF04551 GcpE [H]

EC number: =1.17.7.1 [H]

Molecular weight: Translated: 37647; Mature: 37647

Theoretical pI: Translated: 5.75; Mature: 5.75

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYRDNTKVVKIGKCVIGGGNPILIQSMTNTHTEDVAATVAQIKELTKAGCEIIRCTVPTK
CCCCCCCEEEEEEEEECCCCEEEEEECCCCCHHHHHHHHHHHHHHHHCCCEEEEEECCCH
EAALAIKEIKKQIEIPLVADIHFDYKMAIMAMENGADKIRINPGNIGGTDKIKAVVDVAR
HHHHHHHHHHHHCCCCEEEEEECCEEEEEEEEECCCCEEEECCCCCCCHHHHHHHHHHHH
ERNIPIRVGVNSGSLEKDLIEKYGGVTAEGIVESALDKVKIIEDLGYDNLVVSIKSSDVM
HCCCCEEEECCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCEE
MCVKAHELIAKQTNHPLHVGITESGTIFSGSIKSSIGLGLILNQGIGDTIRVSLTDDPVR
EEHHHHHHHHHCCCCCEEEEECCCCCEEECCHHCCCCEEEEEECCCCCEEEEEECCCHHH
EIEAAKLILKTLGLRKGGIEVVSCPTCGRTRINLIDLAGKVEKMVSGYDLDLKLAVMGCV
HHHHHHHHHHHHCCCCCCEEEEECCCCCCCEEEEEHHHHHHHHHHCCCCCEEEEEEEEEE
VNGPGEAKEADLGIAGGDGCGVLIKHGEIIKKVPEEELLDTLRYELDNWNE
ECCCCCCCCCCCEEECCCCCEEEEECCHHHHHCCHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MYRDNTKVVKIGKCVIGGGNPILIQSMTNTHTEDVAATVAQIKELTKAGCEIIRCTVPTK
CCCCCCCEEEEEEEEECCCCEEEEEECCCCCHHHHHHHHHHHHHHHHCCCEEEEEECCCH
EAALAIKEIKKQIEIPLVADIHFDYKMAIMAMENGADKIRINPGNIGGTDKIKAVVDVAR
HHHHHHHHHHHHCCCCEEEEEECCEEEEEEEEECCCCEEEECCCCCCCHHHHHHHHHHHH
ERNIPIRVGVNSGSLEKDLIEKYGGVTAEGIVESALDKVKIIEDLGYDNLVVSIKSSDVM
HCCCCEEEECCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCEE
MCVKAHELIAKQTNHPLHVGITESGTIFSGSIKSSIGLGLILNQGIGDTIRVSLTDDPVR
EEHHHHHHHHHCCCCCEEEEECCCCCEEECCHHCCCCEEEEEECCCCCEEEEEECCCHHH
EIEAAKLILKTLGLRKGGIEVVSCPTCGRTRINLIDLAGKVEKMVSGYDLDLKLAVMGCV
HHHHHHHHHHHHCCCCCCEEEEECCCCCCCEEEEEHHHHHHHHHHCCCCCEEEEEEEEEE
VNGPGEAKEADLGIAGGDGCGVLIKHGEIIKKVPEEELLDTLRYELDNWNE
ECCCCCCCCCCCEEECCCCCEEEEECCHHHHHCCHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA