Click here to switch to the map view.

The map label for this gene is cysK [H]

Identifier: 238916690

GI number: 238916690

Start: 760741

End: 761706

Strand: Direct

Name: cysK [H]

Synonym: EUBELI_00752

Alternate gene names: 238916690

Gene position: 760741-761706 (Clockwise)

Preceding gene: 238916689

Following gene: 238916691

Centisome position: 35.48

GC content: 41.61

Gene sequence:

>966_bases
ATGTGGAATAATACCACAAGAAAAGAGGATTGTATGGGAAAGATATATAAATCAGCAGCAGATTTAATAGGTAACACACC
ACTTGTAGAAGTCGGACATATTGAAGAGAAGTTTGGACTTAAGGCAAGAGTACTTGTTAAGCTTGAATATTTTAATGCAA
CAGGAAGCGTTAAGGACAGAGTTGCAAAGGCAATGATTGAAGATGCAGAGAAGAAAGGAATTCTTAAGCCGGGCGCAACA
ATTATTGAGCCTACATCAGGCAATACAGGAATCGGACTTGCTGCCATTGCAACAGCAAAGGGCTACAGAACAATAATAGT
TCTTCCAGAGACTATGAGTGTCGAAAGAAGAAATATTATCAAGGCATATGGTGCAGAGATTGTACTTACTCCGGGTTATA
AAGGAATGACAGGTGCTATTGCGAAGGCAGAAGAATTAAAGAATGAGATTGAAGGCAGCATGATTGCAGGACAGTTTGTT
AATCCTGCTAACCCGGATGCACACAGAAGAACAACTGGTCCAGAGATATGGAATGATACGGATGGAGAAGTTGACGCATT
TGTTGCAGGTGTTGGTACAGGAGGAACAATTACAGGTGTTGGTGAATATCTTAAGTCTAAGAATGACAAGATTGAGATAA
TTGCAGTTGAGCCTGCAACTTCACCGGTACTTTCACAGGGCAAGCCAGGACCACATAAGATTCAGGGAATTGGAGCAGGT
TTTGTTCCAGAAATTCTTAACACTAAGATATATGACAGCGTAGTTCCAATCGATAATGATGACGCATTCGAGTATGCCAG
ATTGATATCACACACAGAGGGAATTCTGGTTGGAATATCGGCAGGTGCAGCACTTTACGCAGCAATCGAGTGGGCTAAGA
AACCAGAGAACGAAGGTAAGACAATCGTTGCACTGCTTCCAGACAGCGGCGACAGATATTACTCAACATCACTTTTTGAG
AACTAA

Upstream 100 bases:

>100_bases
ACTGTACAAATCAGAATCATTGTCCTATAATAACATATAAAAATAGTATGTTTATGTACTGCATATACATAACACCTGAT
TCATGAATTGATATTAGAAA

Downstream 100 bases:

>100_bases
TAGCAGAATAAATGTGTCAAATGTGGACATTCTATAAGGGCAGGGCGTAAGTCCTGCCCTTTTCGTGCTTTGACAGGGTT
AGTGAAATTGAGAGTTGATT

Product: cysteine synthase

Products: NA

Alternate protein names: CSase; O-acetylserine (thiol)-lyase; OAS-TL; O-acetylserine sulfhydrylase [H]

Number of amino acids: Translated: 321; Mature: 321

Protein sequence:

>321_residues
MWNNTTRKEDCMGKIYKSAADLIGNTPLVEVGHIEEKFGLKARVLVKLEYFNATGSVKDRVAKAMIEDAEKKGILKPGAT
IIEPTSGNTGIGLAAIATAKGYRTIIVLPETMSVERRNIIKAYGAEIVLTPGYKGMTGAIAKAEELKNEIEGSMIAGQFV
NPANPDAHRRTTGPEIWNDTDGEVDAFVAGVGTGGTITGVGEYLKSKNDKIEIIAVEPATSPVLSQGKPGPHKIQGIGAG
FVPEILNTKIYDSVVPIDNDDAFEYARLISHTEGILVGISAGAALYAAIEWAKKPENEGKTIVALLPDSGDRYYSTSLFE
N

Sequences:

>Translated_321_residues
MWNNTTRKEDCMGKIYKSAADLIGNTPLVEVGHIEEKFGLKARVLVKLEYFNATGSVKDRVAKAMIEDAEKKGILKPGAT
IIEPTSGNTGIGLAAIATAKGYRTIIVLPETMSVERRNIIKAYGAEIVLTPGYKGMTGAIAKAEELKNEIEGSMIAGQFV
NPANPDAHRRTTGPEIWNDTDGEVDAFVAGVGTGGTITGVGEYLKSKNDKIEIIAVEPATSPVLSQGKPGPHKIQGIGAG
FVPEILNTKIYDSVVPIDNDDAFEYARLISHTEGILVGISAGAALYAAIEWAKKPENEGKTIVALLPDSGDRYYSTSLFE
N
>Mature_321_residues
MWNNTTRKEDCMGKIYKSAADLIGNTPLVEVGHIEEKFGLKARVLVKLEYFNATGSVKDRVAKAMIEDAEKKGILKPGAT
IIEPTSGNTGIGLAAIATAKGYRTIIVLPETMSVERRNIIKAYGAEIVLTPGYKGMTGAIAKAEELKNEIEGSMIAGQFV
NPANPDAHRRTTGPEIWNDTDGEVDAFVAGVGTGGTITGVGEYLKSKNDKIEIIAVEPATSPVLSQGKPGPHKIQGIGAG
FVPEILNTKIYDSVVPIDNDDAFEYARLISHTEGILVGISAGAALYAAIEWAKKPENEGKTIVALLPDSGDRYYSTSLFE
N

Specific function: Cysteine biosynthesis. [C]

COG id: COG0031

COG function: function code E; Cysteine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the cysteine synthase/cystathionine beta- synthase family [H]

Homologues:

Organism=Homo sapiens, GI295821202, Length=313, Percent_Identity=45.3674121405751, Blast_Score=257, Evalue=1e-68,
Organism=Homo sapiens, GI295821200, Length=313, Percent_Identity=45.3674121405751, Blast_Score=257, Evalue=1e-68,
Organism=Homo sapiens, GI4557415, Length=313, Percent_Identity=45.3674121405751, Blast_Score=257, Evalue=1e-68,
Organism=Escherichia coli, GI1788754, Length=317, Percent_Identity=52.3659305993691, Blast_Score=294, Evalue=4e-81,
Organism=Escherichia coli, GI2367138, Length=297, Percent_Identity=42.7609427609428, Blast_Score=223, Evalue=1e-59,
Organism=Caenorhabditis elegans, GI17562970, Length=308, Percent_Identity=49.3506493506493, Blast_Score=298, Evalue=2e-81,
Organism=Caenorhabditis elegans, GI115535073, Length=308, Percent_Identity=54.5454545454545, Blast_Score=296, Evalue=7e-81,
Organism=Caenorhabditis elegans, GI17535051, Length=306, Percent_Identity=47.3856209150327, Blast_Score=294, Evalue=4e-80,
Organism=Caenorhabditis elegans, GI32566674, Length=300, Percent_Identity=50, Blast_Score=291, Evalue=4e-79,
Organism=Caenorhabditis elegans, GI17561720, Length=302, Percent_Identity=47.3509933774834, Blast_Score=255, Evalue=2e-68,
Organism=Caenorhabditis elegans, GI32566672, Length=214, Percent_Identity=49.0654205607477, Blast_Score=212, Evalue=2e-55,
Organism=Caenorhabditis elegans, GI17534315, Length=333, Percent_Identity=40.2402402402402, Blast_Score=202, Evalue=2e-52,
Organism=Caenorhabditis elegans, GI25147552, Length=322, Percent_Identity=38.5093167701863, Blast_Score=180, Evalue=1e-45,
Organism=Caenorhabditis elegans, GI17561716, Length=118, Percent_Identity=41.5254237288136, Blast_Score=96, Evalue=3e-20,
Organism=Caenorhabditis elegans, GI71996324, Length=349, Percent_Identity=26.3610315186246, Blast_Score=70, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6321594, Length=311, Percent_Identity=43.4083601286174, Blast_Score=238, Evalue=6e-64,
Organism=Saccharomyces cerevisiae, GI6321449, Length=327, Percent_Identity=34.5565749235474, Blast_Score=130, Evalue=3e-31,
Organism=Drosophila melanogaster, GI24643623, Length=316, Percent_Identity=38.6075949367089, Blast_Score=202, Evalue=3e-52,
Organism=Drosophila melanogaster, GI20129101, Length=316, Percent_Identity=38.6075949367089, Blast_Score=202, Evalue=3e-52,

Paralogues:

None

Copy number: 2820 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001216
- InterPro:   IPR005856
- InterPro:   IPR005859
- InterPro:   IPR001926 [H]

Pfam domain/function: PF00291 PALP [H]

EC number: =2.5.1.47 [H]

Molecular weight: Translated: 34280; Mature: 34280

Theoretical pI: Translated: 5.13; Mature: 5.13

Prosite motif: PS00901 CYS_SYNTHASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MWNNTTRKEDCMGKIYKSAADLIGNTPLVEVGHIEEKFGLKARVLVKLEYFNATGSVKDR
CCCCCCCHHHHHHHHHHHHHHHHCCCCEEEECCCHHHHCCEEEEEEEEEEECCCCCHHHH
VAKAMIEDAEKKGILKPGATIIEPTSGNTGIGLAAIATAKGYRTIIVLPETMSVERRNII
HHHHHHHHHHHCCCCCCCCEEEECCCCCCCCCEEEEEECCCCEEEEEECCCCCHHHHHHH
KAYGAEIVLTPGYKGMTGAIAKAEELKNEIEGSMIAGQFVNPANPDAHRRTTGPEIWNDT
HHCCCEEEECCCCCCCHHHHHHHHHHHHHHCCCEEECCCCCCCCCCCCCCCCCCCCCCCC
DGEVDAFVAGVGTGGTITGVGEYLKSKNDKIEIIAVEPATSPVLSQGKPGPHKIQGIGAG
CCCEEEEEEECCCCCCHHHHHHHHHCCCCEEEEEEECCCCCCHHHCCCCCCCEEEECCCC
FVPEILNTKIYDSVVPIDNDDAFEYARLISHTEGILVGISAGAALYAAIEWAKKPENEGK
HHHHHHCCHHHHCCCCCCCCCHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHCCCCCCC
TIVALLPDSGDRYYSTSLFEN
EEEEEECCCCCCEEECCCCCC
>Mature Secondary Structure
MWNNTTRKEDCMGKIYKSAADLIGNTPLVEVGHIEEKFGLKARVLVKLEYFNATGSVKDR
CCCCCCCHHHHHHHHHHHHHHHHCCCCEEEECCCHHHHCCEEEEEEEEEEECCCCCHHHH
VAKAMIEDAEKKGILKPGATIIEPTSGNTGIGLAAIATAKGYRTIIVLPETMSVERRNII
HHHHHHHHHHHCCCCCCCCEEEECCCCCCCCCEEEEEECCCCEEEEEECCCCCHHHHHHH
KAYGAEIVLTPGYKGMTGAIAKAEELKNEIEGSMIAGQFVNPANPDAHRRTTGPEIWNDT
HHCCCEEEECCCCCCCHHHHHHHHHHHHHHCCCEEECCCCCCCCCCCCCCCCCCCCCCCC
DGEVDAFVAGVGTGGTITGVGEYLKSKNDKIEIIAVEPATSPVLSQGKPGPHKIQGIGAG
CCCEEEEEEECCCCCCHHHHHHHHHCCCCEEEEEEECCCCCCHHHCCCCCCCEEEECCCC
FVPEILNTKIYDSVVPIDNDDAFEYARLISHTEGILVGISAGAALYAAIEWAKKPENEGK
HHHHHHCCHHHHCCCCCCCCCHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHCCCCCCC
TIVALLPDSGDRYYSTSLFEN
EEEEEECCCCCCEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA