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The map label for this gene is amtB [C]

Identifier: 238916678

GI number: 238916678

Start: 746116

End: 747864

Strand: Direct

Name: amtB [C]

Synonym: EUBELI_00740

Alternate gene names: 238916678

Gene position: 746116-747864 (Clockwise)

Preceding gene: 238916676

Following gene: 238916679

Centisome position: 34.8

GC content: 40.48

Gene sequence:

>1749_bases
ATGGTTATGCAAGAGATTTTATCAGCGGTAGATGCCGAATTATTCGGTGTGTGGTTCCTTATAGGTGCAGCTCTTGTTTT
CTGGATGCAGGCAGGCTTTGCAATGGTAGAAACAGGATTCACAAGAGCAAAGAATTCAGGTAACATTATTATGAAGAATC
TGATGGACTTCTGTATTGGTACAGTAATGTTCGTTCTTATTGGATTCAGTTTCTTATTAGGTGAGGATTTACTTGGTTTT
ATTGGAAAGCCTGGTTTTGATATCTTCACAGCTTACAAAGATTTTGATTTTTCTAGTTTCGTATTTAACTTAGTATTCTG
TGCTACAACAGCTACTATTGTTTCAGGTGCCATGGCTGAGAGAACAAAGTTCTTATCATATTGTGTATACTCAGCAGTTA
TCTCAGCACTTATCTATCCAATAGAAGCTCACTGGATCTGGGGCGGGGGCTGGCTTGCACAGTTAGGCTTCCATGATTTT
GCAGGTTCTTGTTGTATTCACATGGTTGGTGGTATTTCAGCTCTTATTGGTGCTAAGATTCTTGGACCTCGTATTGGTAA
GTTTGAAAAGGATGCTAATGGTAAGGTTATAAAGGTAAATGCTTTCCCAGGACACAATATTCCACTTGGTGCACTTGGTG
TATTTATTCTGTGGTTTGGCTGGTATGGATTTAACGGTGCAGCAGCAACAACAATTGAAGATTTAGGCTCTATTTTCCTT
ACAACAACAGTTGCTCCTGCAGTAGCTACAGTAACATGTATGATATTCACATGGATAAGATATGGTAAGCCTGATGTTTC
AATGTGTTTAAATGCTTCACTTGCAGGTCTTGTTGGTATAACAGCTCCTTGTGATGTAACAGATGCATTTGGTGCAACAA
TGATAGGTATTGTTTCTGGTCTTTTAGTAGTATTCGGCGTATGGCTTCTTGACTACAAGCTTCATGTTGATGATCCTGTA
GGTGCTGTAGCAGTACATATGATGAATGGTATCTGGGGTACAATTGCTGTTGGCCTTTTTGCAACAAGCTCAGCTCCGGG
ATATGCAATTGCTTTAGAAGGTGGTTCAATTAAGGGCGAAGGTCTTTTCTATGGCGGTGGATTCACACAGCTTGGACTTC
AGTTATTAGGATTTGTATCAGTTGCAGCATGGGCAGTTGTATGCATGGTGATTGTGTTCACAGTAATTAAGGCTACAATC
GGACTCAGAGCTTCTAAGGAAGAAGAAATCAGAGGCCTTGATATTATGGAGCATGGCCTTTCAAGTGCTTATGCAGGATT
TGAATTCGGTGGTATGGACTTTGTTGACGGTGATGTTGATGTAATCGGTTCAGAATCAATGGAAGCTTCTGTACCTGCTC
TTGTTAAGACATCTGATGCAGGTGATGGCAAGAAGATTACCAAAGTTGAGATTCTTATGAAGCAGGAGAGATTCGAGAGA
TTCAAGAAGGCCATGAACGATATCGGAGTTACAGGTATGACAGTTACACAGGTTCTTGGATGTGGTACACAGAAGGGTGC
TCCTGAATATTACAGAGGTGTTCCAATGGATATCCAGCTTCTTCCTAAGACACAGGTTGAGATGGTAATATCTTCAGTTC
CTGTAATGGATGTTATCAATGCTACAAGAAAGGCTCTCTATACAGGTCATATCGGTGATGGTAAGATTTTTGTATATGAT
GTTGAGGATGTAGTAAAGGTAAGAACCGGAGAATCAGGTTATGATGCACTTCAGGGTGAAGACGATTAA

Upstream 100 bases:

>100_bases
TGCCTACACGCCTTTTTTGCATTCTGTTTAATCAGGTACAGAACCTGATCAACTCTGTTACCGGCAATTAAATTAAAAAG
AATTTCTGATTAGGGAGGAA

Downstream 100 bases:

>100_bases
TATAATGATTTATTAATAAAATGTAGTAATAATAAGAATATGTAAATCGTGGAACTGGACTTTTCCGATGCTTGGACATT
GGAAAAGTCCAGTTTTTAAT

Product: guanylate cyclase

Products: NH3 [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 582; Mature: 582

Protein sequence:

>582_residues
MVMQEILSAVDAELFGVWFLIGAALVFWMQAGFAMVETGFTRAKNSGNIIMKNLMDFCIGTVMFVLIGFSFLLGEDLLGF
IGKPGFDIFTAYKDFDFSSFVFNLVFCATTATIVSGAMAERTKFLSYCVYSAVISALIYPIEAHWIWGGGWLAQLGFHDF
AGSCCIHMVGGISALIGAKILGPRIGKFEKDANGKVIKVNAFPGHNIPLGALGVFILWFGWYGFNGAAATTIEDLGSIFL
TTTVAPAVATVTCMIFTWIRYGKPDVSMCLNASLAGLVGITAPCDVTDAFGATMIGIVSGLLVVFGVWLLDYKLHVDDPV
GAVAVHMMNGIWGTIAVGLFATSSAPGYAIALEGGSIKGEGLFYGGGFTQLGLQLLGFVSVAAWAVVCMVIVFTVIKATI
GLRASKEEEIRGLDIMEHGLSSAYAGFEFGGMDFVDGDVDVIGSESMEASVPALVKTSDAGDGKKITKVEILMKQERFER
FKKAMNDIGVTGMTVTQVLGCGTQKGAPEYYRGVPMDIQLLPKTQVEMVISSVPVMDVINATRKALYTGHIGDGKIFVYD
VEDVVKVRTGESGYDALQGEDD

Sequences:

>Translated_582_residues
MVMQEILSAVDAELFGVWFLIGAALVFWMQAGFAMVETGFTRAKNSGNIIMKNLMDFCIGTVMFVLIGFSFLLGEDLLGF
IGKPGFDIFTAYKDFDFSSFVFNLVFCATTATIVSGAMAERTKFLSYCVYSAVISALIYPIEAHWIWGGGWLAQLGFHDF
AGSCCIHMVGGISALIGAKILGPRIGKFEKDANGKVIKVNAFPGHNIPLGALGVFILWFGWYGFNGAAATTIEDLGSIFL
TTTVAPAVATVTCMIFTWIRYGKPDVSMCLNASLAGLVGITAPCDVTDAFGATMIGIVSGLLVVFGVWLLDYKLHVDDPV
GAVAVHMMNGIWGTIAVGLFATSSAPGYAIALEGGSIKGEGLFYGGGFTQLGLQLLGFVSVAAWAVVCMVIVFTVIKATI
GLRASKEEEIRGLDIMEHGLSSAYAGFEFGGMDFVDGDVDVIGSESMEASVPALVKTSDAGDGKKITKVEILMKQERFER
FKKAMNDIGVTGMTVTQVLGCGTQKGAPEYYRGVPMDIQLLPKTQVEMVISSVPVMDVINATRKALYTGHIGDGKIFVYD
VEDVVKVRTGESGYDALQGEDD
>Mature_582_residues
MVMQEILSAVDAELFGVWFLIGAALVFWMQAGFAMVETGFTRAKNSGNIIMKNLMDFCIGTVMFVLIGFSFLLGEDLLGF
IGKPGFDIFTAYKDFDFSSFVFNLVFCATTATIVSGAMAERTKFLSYCVYSAVISALIYPIEAHWIWGGGWLAQLGFHDF
AGSCCIHMVGGISALIGAKILGPRIGKFEKDANGKVIKVNAFPGHNIPLGALGVFILWFGWYGFNGAAATTIEDLGSIFL
TTTVAPAVATVTCMIFTWIRYGKPDVSMCLNASLAGLVGITAPCDVTDAFGATMIGIVSGLLVVFGVWLLDYKLHVDDPV
GAVAVHMMNGIWGTIAVGLFATSSAPGYAIALEGGSIKGEGLFYGGGFTQLGLQLLGFVSVAAWAVVCMVIVFTVIKATI
GLRASKEEEIRGLDIMEHGLSSAYAGFEFGGMDFVDGDVDVIGSESMEASVPALVKTSDAGDGKKITKVEILMKQERFER
FKKAMNDIGVTGMTVTQVLGCGTQKGAPEYYRGVPMDIQLLPKTQVEMVISSVPVMDVINATRKALYTGHIGDGKIFVYD
VEDVVKVRTGESGYDALQGEDD

Specific function: Involved In The Uptake Of Ammonia (Probable). [C]

COG id: COG0004

COG function: function code P; Ammonia permease

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ammonium transporter (TC 2.A.49) family [H]

Homologues:

Organism=Escherichia coli, GI1786656, Length=414, Percent_Identity=33.0917874396135, Blast_Score=182, Evalue=6e-47,
Organism=Escherichia coli, GI1786655, Length=109, Percent_Identity=42.2018348623853, Blast_Score=96, Evalue=5e-21,
Organism=Escherichia coli, GI1788904, Length=107, Percent_Identity=40.1869158878505, Blast_Score=89, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI17550180, Length=514, Percent_Identity=31.5175097276265, Blast_Score=235, Evalue=4e-62,
Organism=Caenorhabditis elegans, GI17550182, Length=450, Percent_Identity=35.3333333333333, Blast_Score=233, Evalue=3e-61,
Organism=Caenorhabditis elegans, GI32564042, Length=428, Percent_Identity=32.4766355140187, Blast_Score=201, Evalue=1e-51,
Organism=Caenorhabditis elegans, GI133931100, Length=428, Percent_Identity=30.3738317757009, Blast_Score=183, Evalue=2e-46,
Organism=Saccharomyces cerevisiae, GI6324187, Length=425, Percent_Identity=28.2352941176471, Blast_Score=135, Evalue=2e-32,
Organism=Saccharomyces cerevisiae, GI6321559, Length=414, Percent_Identity=28.0193236714976, Blast_Score=124, Evalue=6e-29,
Organism=Saccharomyces cerevisiae, GI6325396, Length=410, Percent_Identity=28.0487804878049, Blast_Score=123, Evalue=6e-29,
Organism=Drosophila melanogaster, GI161078314, Length=498, Percent_Identity=33.9357429718875, Blast_Score=215, Evalue=8e-56,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001905
- InterPro:   IPR018047 [H]

Pfam domain/function: PF00909 Ammonium_transp [H]

EC number: NA

Molecular weight: Translated: 62199; Mature: 62199

Theoretical pI: Translated: 4.66; Mature: 4.66

Prosite motif: PS00638 PII_GLNB_CTER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVMQEILSAVDAELFGVWFLIGAALVFWMQAGFAMVETGFTRAKNSGNIIMKNLMDFCIG
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCHHHCCCCCCHHHHHHHHHHHH
TVMFVLIGFSFLLGEDLLGFIGKPGFDIFTAYKDFDFSSFVFNLVFCATTATIVSGAMAE
HHHHHHHHHHHHHHHHHHHHHCCCCCCEEEHHCCCCHHHHHHHHHHHHHHHHHHHHHHHH
RTKFLSYCVYSAVISALIYPIEAHWIWGGGWLAQLGFHDFAGSCCIHMVGGISALIGAKI
HHHHHHHHHHHHHHHHHHHHHEEEEEECCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHH
LGPRIGKFEKDANGKVIKVNAFPGHNIPLGALGVFILWFGWYGFNGAAATTIEDLGSIFL
HCCCCCCCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHH
TTTVAPAVATVTCMIFTWIRYGKPDVSMCLNASLAGLVGITAPCDVTDAFGATMIGIVSG
HHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCHHHHHHCCCCCCCCHHHHHHHHHHHHHH
LLVVFGVWLLDYKLHVDDPVGAVAVHMMNGIWGTIAVGLFATSSAPGYAIALEGGSIKGE
HHHHHHHHHHHEEEECCCCHHHHHHHHHHHHHHHHHHHHHEECCCCCEEEEEECCCCCCC
GLFYGGGFTQLGLQLLGFVSVAAWAVVCMVIVFTVIKATIGLRASKEEEIRGLDIMEHGL
EEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCHHHHHHHH
SSAYAGFEFGGMDFVDGDVDVIGSESMEASVPALVKTSDAGDGKKITKVEILMKQERFER
HHHHCCCCCCCCCEECCCCEEECCCCCCCCCCEEEEECCCCCCCEEHHHHHHHHHHHHHH
FKKAMNDIGVTGMTVTQVLGCGTQKGAPEYYRGVPMDIQLLPKTQVEMVISSVPVMDVIN
HHHHHHHCCCCHHHHHHHHCCCCCCCCCHHHCCCCCCEEECCCHHHHHHHHCCCHHHHHH
ATRKALYTGHIGDGKIFVYDVEDVVKVRTGESGYDALQGEDD
HHHHHHEECCCCCCEEEEEEHHHHHHCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MVMQEILSAVDAELFGVWFLIGAALVFWMQAGFAMVETGFTRAKNSGNIIMKNLMDFCIG
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCHHHCCCCCCHHHHHHHHHHHH
TVMFVLIGFSFLLGEDLLGFIGKPGFDIFTAYKDFDFSSFVFNLVFCATTATIVSGAMAE
HHHHHHHHHHHHHHHHHHHHHCCCCCCEEEHHCCCCHHHHHHHHHHHHHHHHHHHHHHHH
RTKFLSYCVYSAVISALIYPIEAHWIWGGGWLAQLGFHDFAGSCCIHMVGGISALIGAKI
HHHHHHHHHHHHHHHHHHHHHEEEEEECCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHH
LGPRIGKFEKDANGKVIKVNAFPGHNIPLGALGVFILWFGWYGFNGAAATTIEDLGSIFL
HCCCCCCCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHH
TTTVAPAVATVTCMIFTWIRYGKPDVSMCLNASLAGLVGITAPCDVTDAFGATMIGIVSG
HHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCHHHHHHCCCCCCCCHHHHHHHHHHHHHH
LLVVFGVWLLDYKLHVDDPVGAVAVHMMNGIWGTIAVGLFATSSAPGYAIALEGGSIKGE
HHHHHHHHHHHEEEECCCCHHHHHHHHHHHHHHHHHHHHHEECCCCCEEEEEECCCCCCC
GLFYGGGFTQLGLQLLGFVSVAAWAVVCMVIVFTVIKATIGLRASKEEEIRGLDIMEHGL
EEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCHHHHHHHH
SSAYAGFEFGGMDFVDGDVDVIGSESMEASVPALVKTSDAGDGKKITKVEILMKQERFER
HHHHCCCCCCCCCEECCCCEEECCCCCCCCCCEEEEECCCCCCCEEHHHHHHHHHHHHHH
FKKAMNDIGVTGMTVTQVLGCGTQKGAPEYYRGVPMDIQLLPKTQVEMVISSVPVMDVIN
HHHHHHHCCCCHHHHHHHHCCCCCCCCCHHHCCCCCCEEECCCHHHHHHHHCCCHHHHHH
ATRKALYTGHIGDGKIFVYDVEDVVKVRTGESGYDALQGEDD
HHHHHHEECCCCCCEEEEEEHHHHHHCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NH3 [Periplasm] [C]

Specific reaction: NH3 [Periplasm] = NH3 [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8590279; 8905231 [H]