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The map label for this gene is mraW

Identifier: 238916664

GI number: 238916664

Start: 729549

End: 730496

Strand: Direct

Name: mraW

Synonym: EUBELI_00726

Alternate gene names: 238916664

Gene position: 729549-730496 (Clockwise)

Preceding gene: 238916663

Following gene: 238916665

Centisome position: 34.02

GC content: 37.24

Gene sequence:

>948_bases
ATGGGGTTTATTATGGAATTTAAACACAAATCAGTTCTTTTGGAAGAAACAATTGACAATCTGAATATTAAACCTGATGG
AATCTATGTTGATGGAACACTTGGCGGTGCAGGACATTCATATCAGATAGCGAAAAGACTTACAGGTGGTGGCAGACTTA
TAGGTATAGATCAGGACGCGGACGCAATTGCGGCAGCGACAGAAAGACTTAAAGAATTTGAAGAACGCGTAACAATAGTA
AGAAATAATTATTGCAATATGGACAAGGTTCTTGATGAACTTGGAATTGATAAAGTAGATGGAATACTTCTTGATATAGG
AGTTTCATCGTATCAGTTAGATACAGCAAGCAGAGGATTTACTTATAATGTAGATACTGCTCTTGATATGAGAATGGATC
AGAGACAGGAGATGACAGCAAAGGATCTTGTTAATACATATAGTGAGATGGAACTTTTCAGAATCATAAGGGATTATGGA
GAGGACAGATTTGCCAAGAATATTGCTAAACACATAGTTGCGGCAAGAAAAGAAAAGCCGATAGAAACAACATTTGAACT
AAATGAGATTATAAAGGCTTCTATTCCGGCAAAGGTGAGGGCGACAGGCGGTCATCCATCAAAGAGAACATACCAGGCTA
TAAGAATTGAACTTAACAGGGAATTAGATGTCTTAGAGAATTCAATTGATATGATGATAGACAGGCTTAATCCACAGGGG
CGTTTATGTATCATAACATTCCATTCACTTGAGGACAGAATAGTTAAGGCAAGATTTAGAAACAATGAGAATCCATGTAC
ATGTCCACCGGATTTCCCAGTATGTGTATGCGGAAAGAAATCAAAAGGAAAGGTTATAACAAGAAAACCTATAGTTCCCG
GAGATGAAGAACTAAACGAGAATCAGAGGTCTAAAAGCTCTAAACTGAGGGTGTTTGAAAGAATATAA

Upstream 100 bases:

>100_bases
CAAAGACCGCTGGCTGCAGAGTATCAGTACCTATGATGATGATATGGATGAAGTAGCAGCTAATATGGAGAGTCTTGGAT
TTTCTATATAATATGCAGAC

Downstream 100 bases:

>100_bases
AACTTTTATGGAGGGGAAAGTTTTGGAGAAGAGAAGAAATGTTGTAAATCACAGGACAACTGAATATCAGTATGGAAGTG
CAGTAAGAAAGCTGCAGTCT

Product: S-adenosyl-methyltransferase MraW

Products: NA

Alternate protein names: 16S rRNA m(4)C1402 methyltransferase; rRNA (cytosine-N(4)-)-methyltransferase RsmH

Number of amino acids: Translated: 315; Mature: 314

Protein sequence:

>315_residues
MGFIMEFKHKSVLLEETIDNLNIKPDGIYVDGTLGGAGHSYQIAKRLTGGGRLIGIDQDADAIAAATERLKEFEERVTIV
RNNYCNMDKVLDELGIDKVDGILLDIGVSSYQLDTASRGFTYNVDTALDMRMDQRQEMTAKDLVNTYSEMELFRIIRDYG
EDRFAKNIAKHIVAARKEKPIETTFELNEIIKASIPAKVRATGGHPSKRTYQAIRIELNRELDVLENSIDMMIDRLNPQG
RLCIITFHSLEDRIVKARFRNNENPCTCPPDFPVCVCGKKSKGKVITRKPIVPGDEELNENQRSKSSKLRVFERI

Sequences:

>Translated_315_residues
MGFIMEFKHKSVLLEETIDNLNIKPDGIYVDGTLGGAGHSYQIAKRLTGGGRLIGIDQDADAIAAATERLKEFEERVTIV
RNNYCNMDKVLDELGIDKVDGILLDIGVSSYQLDTASRGFTYNVDTALDMRMDQRQEMTAKDLVNTYSEMELFRIIRDYG
EDRFAKNIAKHIVAARKEKPIETTFELNEIIKASIPAKVRATGGHPSKRTYQAIRIELNRELDVLENSIDMMIDRLNPQG
RLCIITFHSLEDRIVKARFRNNENPCTCPPDFPVCVCGKKSKGKVITRKPIVPGDEELNENQRSKSSKLRVFERI
>Mature_314_residues
GFIMEFKHKSVLLEETIDNLNIKPDGIYVDGTLGGAGHSYQIAKRLTGGGRLIGIDQDADAIAAATERLKEFEERVTIVR
NNYCNMDKVLDELGIDKVDGILLDIGVSSYQLDTASRGFTYNVDTALDMRMDQRQEMTAKDLVNTYSEMELFRIIRDYGE
DRFAKNIAKHIVAARKEKPIETTFELNEIIKASIPAKVRATGGHPSKRTYQAIRIELNRELDVLENSIDMMIDRLNPQGR
LCIITFHSLEDRIVKARFRNNENPCTCPPDFPVCVCGKKSKGKVITRKPIVPGDEELNENQRSKSSKLRVFERI

Specific function: Specifically methylates the N4 position of cytidine in position 1402 (C1402) of 16S rRNA

COG id: COG0275

COG function: function code M; Predicted S-adenosylmethionine-dependent methyltransferase involved in cell envelope biogenesis

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RsmH family

Homologues:

Organism=Homo sapiens, GI165377209, Length=337, Percent_Identity=31.7507418397626, Blast_Score=149, Evalue=3e-36,
Organism=Homo sapiens, GI165377202, Length=188, Percent_Identity=34.5744680851064, Blast_Score=104, Evalue=1e-22,
Organism=Escherichia coli, GI1786270, Length=312, Percent_Identity=43.2692307692308, Blast_Score=245, Evalue=4e-66,
Organism=Drosophila melanogaster, GI62472493, Length=326, Percent_Identity=34.6625766871166, Blast_Score=152, Evalue=2e-37,
Organism=Drosophila melanogaster, GI28571637, Length=326, Percent_Identity=34.6625766871166, Blast_Score=152, Evalue=2e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RSMH_EUBE2 (C4Z530)

Other databases:

- EMBL:   CP001104
- RefSeq:   YP_002930181.1
- ProteinModelPortal:   C4Z530
- GeneID:   7957271
- GenomeReviews:   CP001104_GR
- KEGG:   eel:EUBELI_00726
- OMA:   RPINSTL
- ProtClustDB:   PRK00050
- GO:   GO:0005737
- HAMAP:   MF_01007
- InterPro:   IPR002903
- PANTHER:   PTHR11265
- PIRSF:   PIRSF004486
- TIGRFAMs:   TIGR00006

Pfam domain/function: PF01795 Methyltransf_5

EC number: NA

Molecular weight: Translated: 35685; Mature: 35554

Theoretical pI: Translated: 7.01; Mature: 7.01

Prosite motif: NA

Important sites: BINDING 57-57 BINDING 84-84 BINDING 105-105 BINDING 112-112

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGFIMEFKHKSVLLEETIDNLNIKPDGIYVDGTLGGAGHSYQIAKRLTGGGRLIGIDQDA
CCCEEECCCHHHHHHHHHHCCCCCCCCEEEECCCCCCCCCHHHHHHHCCCCEEEEECCCC
DAIAAATERLKEFEERVTIVRNNYCNMDKVLDELGIDKVDGILLDIGVSSYQLDTASRGF
HHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCHHCCEEEEECCCCEEECCCCCCE
TYNVDTALDMRMDQRQEMTAKDLVNTYSEMELFRIIRDYGEDRFAKNIAKHIVAARKEKP
EEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHCCCC
IETTFELNEIIKASIPAKVRATGGHPSKRTYQAIRIELNRELDVLENSIDMMIDRLNPQG
CHHHHHHHHHHHHCCCCEEEECCCCCCHHCEEEEEEEECCCHHHHHHHHHHHHHCCCCCC
RLCIITFHSLEDRIVKARFRNNENPCTCPPDFPVCVCGKKSKGKVITRKPIVPGDEELNE
CEEEEEECCHHHHHHHHHHCCCCCCCCCCCCCCEEEECCCCCCCEEEECCCCCCHHHHCH
NQRSKSSKLRVFERI
HHHHHHHHHHHHHCC
>Mature Secondary Structure 
GFIMEFKHKSVLLEETIDNLNIKPDGIYVDGTLGGAGHSYQIAKRLTGGGRLIGIDQDA
CCEEECCCHHHHHHHHHHCCCCCCCCEEEECCCCCCCCCHHHHHHHCCCCEEEEECCCC
DAIAAATERLKEFEERVTIVRNNYCNMDKVLDELGIDKVDGILLDIGVSSYQLDTASRGF
HHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCHHCCEEEEECCCCEEECCCCCCE
TYNVDTALDMRMDQRQEMTAKDLVNTYSEMELFRIIRDYGEDRFAKNIAKHIVAARKEKP
EEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHCCCC
IETTFELNEIIKASIPAKVRATGGHPSKRTYQAIRIELNRELDVLENSIDMMIDRLNPQG
CHHHHHHHHHHHHCCCCEEEECCCCCCHHCEEEEEEEECCCHHHHHHHHHHHHHCCCCCC
RLCIITFHSLEDRIVKARFRNNENPCTCPPDFPVCVCGKKSKGKVITRKPIVPGDEELNE
CEEEEEECCHHHHHHHHHHCCCCCCCCCCCCCCEEEECCCCCCCEEEECCCCCCHHHHCH
NQRSKSSKLRVFERI
HHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA