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The map label for this gene is dapL

Identifier: 238916615

GI number: 238916615

Start: 670587

End: 671801

Strand: Direct

Name: dapL

Synonym: EUBELI_00672

Alternate gene names: 238916615

Gene position: 670587-671801 (Clockwise)

Preceding gene: 238916614

Following gene: 238916617

Centisome position: 31.27

GC content: 40.08

Gene sequence:

>1215_bases
ATGTTTAAGATTAACGATAATTATTTAAAGCTTCCAGGAAGCTATTTATTTTCAACAATCGGTAAGAAAGTAGCAGCTTA
TCAGCAGGCTAATCCAGATAAGGAAATCATCAGACTTGGTATTGGCGATGTTACACAGCCATTAGCACCGGCAGTAATTG
ATGCATTACATAAGTCAGTTGACGAGATGGGACATGCCGAGACTTTCCACGGATATGCACCAGATCTTGGATATGAATTC
TTAAGAAGTGCAATTGCAGACCATGATTACAAGAAGCGCGGATGTGATATATCAGCAGATGAGATATTCGTATCAGATGG
AGCAAAGTCTGATTCAGGTAATATCGGAGATATCTTCTCAGTAGATAATAAGATTGCAGTATGTGACCCTGTATATCCTG
TATATGTTGATACTAATGCAATGGCAGGAAGAACAGGAGATTATATTCCAGAGAAGCAGGCATGGAGTAATGTTGTTTAT
ATGCCTTGTACAGCAGAGACTAACTTTGCACCAGAACTTCCAAAAGAAACACCAGATATTATATATCTCTGCTTCCCTAA
CAATCCAACAGGTTCAACAATCACTAAGGACGAACTTCAGAAGTGGGTTGATTATGCTAATAAGGTTGGTGCTGTTATTA
TCTATGATGCAGCATATGAAGCATACATTTCAGAACCAGATGTTCCTCATACAATATATGAGTGTGAAGGTGCAAGAACA
TGTGCAATCGAACTCAGGTCATTCTCTAAGAATGCAGGATTTACAGGTGTAAGACTTGGCTTCACAGTTATTCCTAAGGA
TCTTAAGTGTGGAGATGTAACACTTCACAGCCTCTGGGCAAGAAGACATGGAACTAAGTTTAACGGAGCTCCTTACATTG
TACAGAGAGCAGGCGAGGCAGTTTATTCAGAAGCTGGACAGAAGCAGACAGGTGAGCAGATTGCTTACTACATGAACAAT
GCAAAGACAATTCTTGAAGGACTTAAGTCAGCAGGATATACAGTATCAGGTGGTGTTAATGCACCATATATCTGGTTAAA
GACTCCAGATAAGATGACAAGCTGGGAATTCTTCGATTACCTTCTTGAAAAGGCTAATGTTGTTGGTACACCAGGTTCAG
GATTCGGACCAAGTGGTGAAGGATACTTCAGACTGACAGCATTTGGTTCATACGAGAACACAGTTAAGGCACTTGAAAGA
ATCAAGGCGCTTTAA

Upstream 100 bases:

>100_bases
AATATGAAAGATTATCAAGATTTTATCTTGGAACATAATAAAAAATGATGTATAATAAGCGTTAGTTTTGTAAAACAAGG
CAGAAAATAAGGAGGAATAT

Downstream 100 bases:

>100_bases
TCAGTAATATAATAGGTATTAATAATAAAAATGCTGTGCGTTAACAAGATGATGTTAATGCACAGCTTTTTTTATAAAAT
TAATTAGCTTTTTTAGTTTC

Product: L,L-diaminopimelate aminotransferase

Products: NA

Alternate protein names: DAP-AT; DAP-aminotransferase; LL-DAP-aminotransferase

Number of amino acids: Translated: 404; Mature: 404

Protein sequence:

>404_residues
MFKINDNYLKLPGSYLFSTIGKKVAAYQQANPDKEIIRLGIGDVTQPLAPAVIDALHKSVDEMGHAETFHGYAPDLGYEF
LRSAIADHDYKKRGCDISADEIFVSDGAKSDSGNIGDIFSVDNKIAVCDPVYPVYVDTNAMAGRTGDYIPEKQAWSNVVY
MPCTAETNFAPELPKETPDIIYLCFPNNPTGSTITKDELQKWVDYANKVGAVIIYDAAYEAYISEPDVPHTIYECEGART
CAIELRSFSKNAGFTGVRLGFTVIPKDLKCGDVTLHSLWARRHGTKFNGAPYIVQRAGEAVYSEAGQKQTGEQIAYYMNN
AKTILEGLKSAGYTVSGGVNAPYIWLKTPDKMTSWEFFDYLLEKANVVGTPGSGFGPSGEGYFRLTAFGSYENTVKALER
IKAL

Sequences:

>Translated_404_residues
MFKINDNYLKLPGSYLFSTIGKKVAAYQQANPDKEIIRLGIGDVTQPLAPAVIDALHKSVDEMGHAETFHGYAPDLGYEF
LRSAIADHDYKKRGCDISADEIFVSDGAKSDSGNIGDIFSVDNKIAVCDPVYPVYVDTNAMAGRTGDYIPEKQAWSNVVY
MPCTAETNFAPELPKETPDIIYLCFPNNPTGSTITKDELQKWVDYANKVGAVIIYDAAYEAYISEPDVPHTIYECEGART
CAIELRSFSKNAGFTGVRLGFTVIPKDLKCGDVTLHSLWARRHGTKFNGAPYIVQRAGEAVYSEAGQKQTGEQIAYYMNN
AKTILEGLKSAGYTVSGGVNAPYIWLKTPDKMTSWEFFDYLLEKANVVGTPGSGFGPSGEGYFRLTAFGSYENTVKALER
IKAL
>Mature_404_residues
MFKINDNYLKLPGSYLFSTIGKKVAAYQQANPDKEIIRLGIGDVTQPLAPAVIDALHKSVDEMGHAETFHGYAPDLGYEF
LRSAIADHDYKKRGCDISADEIFVSDGAKSDSGNIGDIFSVDNKIAVCDPVYPVYVDTNAMAGRTGDYIPEKQAWSNVVY
MPCTAETNFAPELPKETPDIIYLCFPNNPTGSTITKDELQKWVDYANKVGAVIIYDAAYEAYISEPDVPHTIYECEGART
CAIELRSFSKNAGFTGVRLGFTVIPKDLKCGDVTLHSLWARRHGTKFNGAPYIVQRAGEAVYSEAGQKQTGEQIAYYMNN
AKTILEGLKSAGYTVSGGVNAPYIWLKTPDKMTSWEFFDYLLEKANVVGTPGSGFGPSGEGYFRLTAFGSYENTVKALER
IKAL

Specific function: Involved in the synthesis of meso-diaminopimelate (m-DAP or DL-DAP), required for both lysine and peptidoglycan biosynthesis. Catalyzes the direct conversion of tetrahydrodipicolinate to LL-diaminopimelate, a reaction that requires three enzymes in E.coli

COG id: COG0436

COG function: function code E; Aspartate/tyrosine/aromatic aminotransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family. LL-diaminopimelate aminotransferase subfamily

Homologues:

Organism=Escherichia coli, GI1788722, Length=409, Percent_Identity=25.9168704156479, Blast_Score=109, Evalue=3e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DAPAT_EUBE2 (C4Z4Y1)

Other databases:

- EMBL:   CP001104
- RefSeq:   YP_002930132.1
- ProteinModelPortal:   C4Z4Y1
- GeneID:   7957303
- GenomeReviews:   CP001104_GR
- KEGG:   eel:EUBELI_00672
- OMA:   VDLIYLC
- ProtClustDB:   PRK07590
- HAMAP:   MF_01642
- InterPro:   IPR004839
- InterPro:   IPR019942
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422
- Gene3D:   G3DSA:3.40.640.10
- Gene3D:   G3DSA:3.90.1150.10
- TIGRFAMs:   TIGR03542

Pfam domain/function: PF00155 Aminotran_1_2; SSF53383 PyrdxlP-dep_Trfase_major

EC number: =2.6.1.83

Molecular weight: Translated: 44387; Mature: 44387

Theoretical pI: Translated: 5.04; Mature: 5.04

Prosite motif: PS00105 AA_TRANSFER_CLASS_1

Important sites: BINDING 42-42 BINDING 72-72 BINDING 75-75 BINDING 109-109 BINDING 132-132 BINDING 188-188 BINDING 188-188 BINDING 216-216 BINDING 219-219 BINDING 247-247 BINDING 249-249 BINDING 258-258 BINDING 288-288 BINDING 384-384

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFKINDNYLKLPGSYLFSTIGKKVAAYQQANPDKEIIRLGIGDVTQPLAPAVIDALHKSV
CEEECCCEEECCHHHHHHHHHHHHHHHHCCCCCHHHEEECCCCHHHHHHHHHHHHHHHHH
DEMGHAETFHGYAPDLGYEFLRSAIADHDYKKRGCDISADEIFVSDGAKSDSGNIGDIFS
HHHCCCCCCCCCCCCHHHHHHHHHHHCCCHHHCCCCCCCCEEEEECCCCCCCCCCCCEEE
VDNKIAVCDPVYPVYVDTNAMAGRTGDYIPEKQAWSNVVYMPCTAETNFAPELPKETPDI
CCCEEEEECCCEEEEEECCCCCCCCCCCCCCHHHHCCEEEEEECCCCCCCCCCCCCCCCE
IYLCFPNNPTGSTITKDELQKWVDYANKVGAVIIYDAAYEAYISEPDVPHTIYECEGART
EEEEECCCCCCCCCCHHHHHHHHHHHHCCCEEEEEECHHHHHCCCCCCCCEEEECCCCEE
CAIELRSFSKNAGFTGVRLGFTVIPKDLKCGDVTLHSLWARRHGTKFNGAPYIVQRAGEA
EEEEEHHCCCCCCCCEEEEEEEECCCCCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHH
VYSEAGQKQTGEQIAYYMNNAKTILEGLKSAGYTVSGGVNAPYIWLKTPDKMTSWEFFDY
HHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCCEEEEECCCCCCHHHHHHH
LLEKANVVGTPGSGFGPSGEGYFRLTAFGSYENTVKALERIKAL
HHHHCCEECCCCCCCCCCCCCEEEEEEECCHHHHHHHHHHHHCC
>Mature Secondary Structure
MFKINDNYLKLPGSYLFSTIGKKVAAYQQANPDKEIIRLGIGDVTQPLAPAVIDALHKSV
CEEECCCEEECCHHHHHHHHHHHHHHHHCCCCCHHHEEECCCCHHHHHHHHHHHHHHHHH
DEMGHAETFHGYAPDLGYEFLRSAIADHDYKKRGCDISADEIFVSDGAKSDSGNIGDIFS
HHHCCCCCCCCCCCCHHHHHHHHHHHCCCHHHCCCCCCCCEEEEECCCCCCCCCCCCEEE
VDNKIAVCDPVYPVYVDTNAMAGRTGDYIPEKQAWSNVVYMPCTAETNFAPELPKETPDI
CCCEEEEECCCEEEEEECCCCCCCCCCCCCCHHHHCCEEEEEECCCCCCCCCCCCCCCCE
IYLCFPNNPTGSTITKDELQKWVDYANKVGAVIIYDAAYEAYISEPDVPHTIYECEGART
EEEEECCCCCCCCCCHHHHHHHHHHHHCCCEEEEEECHHHHHCCCCCCCCEEEECCCCEE
CAIELRSFSKNAGFTGVRLGFTVIPKDLKCGDVTLHSLWARRHGTKFNGAPYIVQRAGEA
EEEEEHHCCCCCCCCEEEEEEEECCCCCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHH
VYSEAGQKQTGEQIAYYMNNAKTILEGLKSAGYTVSGGVNAPYIWLKTPDKMTSWEFFDY
HHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCCEEEEECCCCCCHHHHHHH
LLEKANVVGTPGSGFGPSGEGYFRLTAFGSYENTVKALERIKAL
HHHHCCEECCCCCCCCCCCCCEEEEEEECCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA