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The map label for this gene is murF [H]

Identifier: 238916549

GI number: 238916549

Start: 617611

End: 619020

Strand: Direct

Name: murF [H]

Synonym: EUBELI_00606

Alternate gene names: 238916549

Gene position: 617611-619020 (Clockwise)

Preceding gene: 238916548

Following gene: 238916550

Centisome position: 28.8

GC content: 38.16

Gene sequence:

>1410_bases
ATGAAGGGAATGACCTTAAGAGCCATGACACAAGCTGTTAACGGCATATATCATGGCAATGGAGAAGATTATGATAAAGA
GATTACTGCCATTACGATTGACAGCAGAAAGGTAGCTGAAGGCGGGCTTTTTATTGCCATTAAAGGTGAGAGAAGTGATG
GACATGATTTCATAGGACAATGCTTTGAGAAGGGCGCTGCATGTGTTATTTCTGAAAAGGAGCTGCCAGATGAAGAACAT
TCATATATTCAGGTTGAATCAAGTCTTCAGGCACTAAAGGATCTTGCACTTCTTTACAGAAATAATCTGGATGTCAAGGT
TGTAGGAATCACAGGAAGTGTTGGCAAAACAAGTACCAAGGAAACAATAAGCTCTGTTTTATCTGAAAAGTACAGGGTAT
TAAAGACACTTGGTAATTTCAATAATGAGATTGGGCTTCCACTTACTGTTTTCAGACTGACAGAAGATGATGAAGTTGCT
GTTCTTGAGATGGGAATCAGTGATTTTGGCGAGATGGACAGATTATCTAAGATTGCACAGCCAGACATATGTGTTATCAC
TAATATTGGCCTTTGCCACCTTGAGAATCTTGGAACAAGAGATGGTATACTTAAGGCTAAAACAGAGATATTTAATCATA
TGAATCCGGATGGAATTGTTATTCTTAACGGAGATGACGACAAATTATCAACAATCAGCCAGGTACACGGCAAGCGTCCT
CTTGTGTTCGGAATAAGCAACAAAGACGGGGTATATGCAGATAATATAAAGAGTCTTGGACTTGATGGAACTAGCTTTAC
AATTCATGGCCTTGATACAGCAGATAAATATTCAACGTTTGACATGACAGTACCGGTACCCGGACATCATATGGTATATA
ATGCGATGGCTGCTGCACTCGTTGGTTCTGTGCTTGGTCTTTCATCTCTTGAAATTGAGCGTGGTGTAAGAAATTTAAAG
ACAATTGCAGGAAGAAACAATATTATTAAGGAAAATGGCTTCACAATAATTGATGACTGCTACAATGCCAATCCGGTTTC
AATGAAAGCATCTCTTGATGTTCTTGACACTGCTATTGGAAGAAAAGTTGCTATTCTTGGAAGTATGTTTGAGCTTGGTG
AGAACGAGAAACAGATGCATTACGATGTTGGGATGTATCTTGGAACTAAGGATATTGATGTACTTATAACAGCAGGTGAC
CTTGCTGCACAGATTGCGGCAGGAACAAGAGCATATATTGATACTAATTACAATGCGCATGGTTGTGAAGTACATGATTT
TGAAACAAGAGATGATATGTTAAAAAGTATTGGACATATTCTGAAAACAGGTGATAATATACTTATAAAGGCTTCTCATG
GAATGGAATTTTCCAAAGTTGTAGAGGCTGTTAAAACAATTAATACATAA

Upstream 100 bases:

>100_bases
TGGTGCTTGAGGATATTCCTGCGGATGGAGATATCAAGTATTACAGAGATGAGAATCAGGTACATCATGTGCCTAAGAAA
AGAATTGAGGATATTGTGTT

Downstream 100 bases:

>100_bases
AGGAGAATTATGAGCAGGGTAGTAAGAAGAATAGGGATAATTGCGGTATTTGTACTTGTTATAGGGGCATTTGCCGGATG
TGGAAAGAAAGAGTCAGTTG

Product: UDP-N-acetylmuramoylalanyl-D-glutamyl-2, 6-diaminopimelate--D-alanyl- D-alanine ligase

Products: NA

Alternate protein names: D-alanyl-D-alanine-adding enzyme; UDP-MurNAc-pentapeptide synthetase [H]

Number of amino acids: Translated: 469; Mature: 469

Protein sequence:

>469_residues
MKGMTLRAMTQAVNGIYHGNGEDYDKEITAITIDSRKVAEGGLFIAIKGERSDGHDFIGQCFEKGAACVISEKELPDEEH
SYIQVESSLQALKDLALLYRNNLDVKVVGITGSVGKTSTKETISSVLSEKYRVLKTLGNFNNEIGLPLTVFRLTEDDEVA
VLEMGISDFGEMDRLSKIAQPDICVITNIGLCHLENLGTRDGILKAKTEIFNHMNPDGIVILNGDDDKLSTISQVHGKRP
LVFGISNKDGVYADNIKSLGLDGTSFTIHGLDTADKYSTFDMTVPVPGHHMVYNAMAAALVGSVLGLSSLEIERGVRNLK
TIAGRNNIIKENGFTIIDDCYNANPVSMKASLDVLDTAIGRKVAILGSMFELGENEKQMHYDVGMYLGTKDIDVLITAGD
LAAQIAAGTRAYIDTNYNAHGCEVHDFETRDDMLKSIGHILKTGDNILIKASHGMEFSKVVEAVKTINT

Sequences:

>Translated_469_residues
MKGMTLRAMTQAVNGIYHGNGEDYDKEITAITIDSRKVAEGGLFIAIKGERSDGHDFIGQCFEKGAACVISEKELPDEEH
SYIQVESSLQALKDLALLYRNNLDVKVVGITGSVGKTSTKETISSVLSEKYRVLKTLGNFNNEIGLPLTVFRLTEDDEVA
VLEMGISDFGEMDRLSKIAQPDICVITNIGLCHLENLGTRDGILKAKTEIFNHMNPDGIVILNGDDDKLSTISQVHGKRP
LVFGISNKDGVYADNIKSLGLDGTSFTIHGLDTADKYSTFDMTVPVPGHHMVYNAMAAALVGSVLGLSSLEIERGVRNLK
TIAGRNNIIKENGFTIIDDCYNANPVSMKASLDVLDTAIGRKVAILGSMFELGENEKQMHYDVGMYLGTKDIDVLITAGD
LAAQIAAGTRAYIDTNYNAHGCEVHDFETRDDMLKSIGHILKTGDNILIKASHGMEFSKVVEAVKTINT
>Mature_469_residues
MKGMTLRAMTQAVNGIYHGNGEDYDKEITAITIDSRKVAEGGLFIAIKGERSDGHDFIGQCFEKGAACVISEKELPDEEH
SYIQVESSLQALKDLALLYRNNLDVKVVGITGSVGKTSTKETISSVLSEKYRVLKTLGNFNNEIGLPLTVFRLTEDDEVA
VLEMGISDFGEMDRLSKIAQPDICVITNIGLCHLENLGTRDGILKAKTEIFNHMNPDGIVILNGDDDKLSTISQVHGKRP
LVFGISNKDGVYADNIKSLGLDGTSFTIHGLDTADKYSTFDMTVPVPGHHMVYNAMAAALVGSVLGLSSLEIERGVRNLK
TIAGRNNIIKENGFTIIDDCYNANPVSMKASLDVLDTAIGRKVAILGSMFELGENEKQMHYDVGMYLGTKDIDVLITAGD
LAAQIAAGTRAYIDTNYNAHGCEVHDFETRDDMLKSIGHILKTGDNILIKASHGMEFSKVVEAVKTINT

Specific function: Involved in cell wall formation. Catalyzes the final step in the synthesis of UDP-N-acetylmuramoyl-pentapeptide, the precursor of murein [H]

COG id: COG0770

COG function: function code M; UDP-N-acetylmuramyl pentapeptide synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family [H]

Homologues:

Organism=Escherichia coli, GI1786274, Length=471, Percent_Identity=33.1210191082803, Blast_Score=205, Evalue=4e-54,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR000713
- InterPro:   IPR005863 [H]

Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.10 [H]

Molecular weight: Translated: 50986; Mature: 50986

Theoretical pI: Translated: 4.97; Mature: 4.97

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKGMTLRAMTQAVNGIYHGNGEDYDKEITAITIDSRKVAEGGLFIAIKGERSDGHDFIGQ
CCCCHHHHHHHHHCCEECCCCCCCCCEEEEEEECCCEECCCCEEEEEECCCCCCHHHHHH
CFEKGAACVISEKELPDEEHSYIQVESSLQALKDLALLYRNNLDVKVVGITGSVGKTSTK
HHHCCCEEEEECCCCCCCCCCEEEEHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCHH
ETISSVLSEKYRVLKTLGNFNNEIGLPLTVFRLTEDDEVAVLEMGISDFGEMDRLSKIAQ
HHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCCEEEEEECCHHHHHHHHHHHHCC
PDICVITNIGLCHLENLGTRDGILKAKTEIFNHMNPDGIVILNGDDDKLSTISQVHGKRP
CCEEEEECCCEEEECCCCCCCCHHHHHHHHHHCCCCCCEEEECCCCHHHHHHHHHCCCCC
LVFGISNKDGVYADNIKSLGLDGTSFTIHGLDTADKYSTFDMTVPVPGHHMVYNAMAAAL
EEEEECCCCCEECCCHHHCCCCCCEEEEEECCCCCCCCEEEEEECCCCCHHHHHHHHHHH
VGSVLGLSSLEIERGVRNLKTIAGRNNIIKENGFTIIDDCYNANPVSMKASLDVLDTAIG
HHHHHCCCHHHHHHHHHHHHHHHCCCCEEECCCCEEEECCCCCCCCEEEEHHHHHHHHHC
RKVAILGSMFELGENEKQMHYDVGMYLGTKDIDVLITAGDLAAQIAAGTRAYIDTNYNAH
CHHHHHHHHHHCCCCCCEEEEEEEEEECCCCEEEEEECCHHHHHHHCCCEEEEECCCCCC
GCEVHDFETRDDMLKSIGHILKTGDNILIKASHGMEFSKVVEAVKTINT
CCEECCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHCCC
>Mature Secondary Structure
MKGMTLRAMTQAVNGIYHGNGEDYDKEITAITIDSRKVAEGGLFIAIKGERSDGHDFIGQ
CCCCHHHHHHHHHCCEECCCCCCCCCEEEEEEECCCEECCCCEEEEEECCCCCCHHHHHH
CFEKGAACVISEKELPDEEHSYIQVESSLQALKDLALLYRNNLDVKVVGITGSVGKTSTK
HHHCCCEEEEECCCCCCCCCCEEEEHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCHH
ETISSVLSEKYRVLKTLGNFNNEIGLPLTVFRLTEDDEVAVLEMGISDFGEMDRLSKIAQ
HHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCCEEEEEECCHHHHHHHHHHHHCC
PDICVITNIGLCHLENLGTRDGILKAKTEIFNHMNPDGIVILNGDDDKLSTISQVHGKRP
CCEEEEECCCEEEECCCCCCCCHHHHHHHHHHCCCCCCEEEECCCCHHHHHHHHHCCCCC
LVFGISNKDGVYADNIKSLGLDGTSFTIHGLDTADKYSTFDMTVPVPGHHMVYNAMAAAL
EEEEECCCCCEECCCHHHCCCCCCEEEEEECCCCCCCCEEEEEECCCCCHHHHHHHHHHH
VGSVLGLSSLEIERGVRNLKTIAGRNNIIKENGFTIIDDCYNANPVSMKASLDVLDTAIG
HHHHHCCCHHHHHHHHHHHHHHHCCCCEEECCCCEEEECCCCCCCCEEEEHHHHHHHHHC
RKVAILGSMFELGENEKQMHYDVGMYLGTKDIDVLITAGDLAAQIAAGTRAYIDTNYNAH
CHHHHHHHHHHCCCCCCEEEEEEEEEECCCCEEEEEECCHHHHHHHCCCEEEEECCCCCC
GCEVHDFETRDDMLKSIGHILKTGDNILIKASHGMEFSKVVEAVKTINT
CCEECCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]