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The map label for this gene is ddl [H]

Identifier: 238916547

GI number: 238916547

Start: 615852

End: 617000

Strand: Direct

Name: ddl [H]

Synonym: EUBELI_00604

Alternate gene names: 238916547

Gene position: 615852-617000 (Clockwise)

Preceding gene: 238916546

Following gene: 238916548

Centisome position: 28.72

GC content: 37.95

Gene sequence:

>1149_bases
GTGGATTATATAAATATATTACAATTATCTGACAATTTTACAAAAAATTTACAATTGACGAAAAATGTGAATAACTATGT
GGATATTGTGGAAAAGTTCAGGGATAAAGGAGAAATTATGAGAATAGTAGTTTTAGCAGGTGGAACAAGTACAGAAAGAG
ATGTTTCATTAGTCACAGGATACAGAGTATGCGAGAGTCTTAGAAGAAACGGACATGAAGCAAATATGCTTGATATCTTC
TTAGGTGTTGATGACAGTGAGGTTAAGACATTTTTTACAGAAAAGAATGATTTGGGAGAACTGTCTGACAATTTAAAGAA
GAAAACAGCGGACATTCCGGCAGAGGTTAAGAGAAGAACAGAGGCAAGAGAAAGCTTTTTCGGACGTAATGTATTAGAAT
TGTGTAAAGAAGCAGATATGGTATTTATGGGACTTCATGGAAGCAATGGTGAAGATGGTAAGGTCCAGGCTACATTTGAT
CTGCTTGGAATCAGATATACAGGTACAGATTATTTAAGCAGTGCTATATCAATGAGCAAGGAACTTGCAAAAAAGGTTCT
TGTACCAGAGGGAATTCCTATGCCTAAGGGTGTTACATTACATAAAGGACATAAGATTGAGTATGTTCCATTCCCATGTG
TTGTTAAGCCTTGTTGTGGTGGTTCAAGCGTTGGTGTTTCGATAGCACATAACGAGAAAGAGTTTGAAGATGCACTTGAT
GAAGCATTTTCTTATGAAGATACTGTACTTGTTGAAGAGTTTGTTGACGGAAGAGAATTCTCAGTTGCGGTGCTTGATGG
AAAGGCACTTCCAGTAATTGAGATTGAGCCTCTTGAAGGCTTCTACGATTACAAAAATAAGTACAAGGCTGGCTCTACTA
AGGAGACATGCCCTGCCAATATTCCGGAAGATATTGCAAGCCAGATGCAGTTCTGGGCAGAAAAATGCTGCCAGGCAGCA
GGCGTAACAACATACGCAAGAGTTGATGAACTTCTTGATAAGAATAATAATATATTCTGTCTTGAGATTAATACACTTCC
AGGAATGACTGACACAAGCCTTATTCCTCAGGAGGCAGCAGCAGTAGGTATATCGTATGATGAACTTACAGAGAAGATTA
TCGAGATATCTCTTGCAAAGTATGAATAG

Upstream 100 bases:

>100_bases
GAAGAATTGATTCTTTAAGAAAGGTTTTACAGGCACATACAAAGTATGAAGGAAGCCATTTAAGACCGTAATAATTCGCA
CAGATTCTGCATAAGAATAT

Downstream 100 bases:

>100_bases
AAGAAATGTAAATGTATAATAATGCATTTTTATAAAACGAGGTTAGTTATGTTATGATTAAGGAACTTGTTAAGAAAAAC
AGATCATACAGAAGATTTTA

Product: D-alanine-D-alanine ligase

Products: NA

Alternate protein names: D-Ala-D-Ala ligase; D-alanylalanine synthetase [H]

Number of amino acids: Translated: 382; Mature: 382

Protein sequence:

>382_residues
MDYINILQLSDNFTKNLQLTKNVNNYVDIVEKFRDKGEIMRIVVLAGGTSTERDVSLVTGYRVCESLRRNGHEANMLDIF
LGVDDSEVKTFFTEKNDLGELSDNLKKKTADIPAEVKRRTEARESFFGRNVLELCKEADMVFMGLHGSNGEDGKVQATFD
LLGIRYTGTDYLSSAISMSKELAKKVLVPEGIPMPKGVTLHKGHKIEYVPFPCVVKPCCGGSSVGVSIAHNEKEFEDALD
EAFSYEDTVLVEEFVDGREFSVAVLDGKALPVIEIEPLEGFYDYKNKYKAGSTKETCPANIPEDIASQMQFWAEKCCQAA
GVTTYARVDELLDKNNNIFCLEINTLPGMTDTSLIPQEAAAVGISYDELTEKIIEISLAKYE

Sequences:

>Translated_382_residues
MDYINILQLSDNFTKNLQLTKNVNNYVDIVEKFRDKGEIMRIVVLAGGTSTERDVSLVTGYRVCESLRRNGHEANMLDIF
LGVDDSEVKTFFTEKNDLGELSDNLKKKTADIPAEVKRRTEARESFFGRNVLELCKEADMVFMGLHGSNGEDGKVQATFD
LLGIRYTGTDYLSSAISMSKELAKKVLVPEGIPMPKGVTLHKGHKIEYVPFPCVVKPCCGGSSVGVSIAHNEKEFEDALD
EAFSYEDTVLVEEFVDGREFSVAVLDGKALPVIEIEPLEGFYDYKNKYKAGSTKETCPANIPEDIASQMQFWAEKCCQAA
GVTTYARVDELLDKNNNIFCLEINTLPGMTDTSLIPQEAAAVGISYDELTEKIIEISLAKYE
>Mature_382_residues
MDYINILQLSDNFTKNLQLTKNVNNYVDIVEKFRDKGEIMRIVVLAGGTSTERDVSLVTGYRVCESLRRNGHEANMLDIF
LGVDDSEVKTFFTEKNDLGELSDNLKKKTADIPAEVKRRTEARESFFGRNVLELCKEADMVFMGLHGSNGEDGKVQATFD
LLGIRYTGTDYLSSAISMSKELAKKVLVPEGIPMPKGVTLHKGHKIEYVPFPCVVKPCCGGSSVGVSIAHNEKEFEDALD
EAFSYEDTVLVEEFVDGREFSVAVLDGKALPVIEIEPLEGFYDYKNKYKAGSTKETCPANIPEDIASQMQFWAEKCCQAA
GVTTYARVDELLDKNNNIFCLEINTLPGMTDTSLIPQEAAAVGISYDELTEKIIEISLAKYE

Specific function: Cell wall formation [H]

COG id: COG1181

COG function: function code M; D-alanine-D-alanine ligase and related ATP-grasp enzymes

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Escherichia coli, GI1786280, Length=348, Percent_Identity=32.4712643678161, Blast_Score=184, Evalue=6e-48,
Organism=Escherichia coli, GI1786579, Length=362, Percent_Identity=29.5580110497238, Blast_Score=148, Evalue=5e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR000291
- InterPro:   IPR005905
- InterPro:   IPR011095
- InterPro:   IPR011127
- InterPro:   IPR013817
- InterPro:   IPR016185 [H]

Pfam domain/function: PF07478 Dala_Dala_lig_C; PF01820 Dala_Dala_lig_N [H]

EC number: =6.3.2.4 [H]

Molecular weight: Translated: 42464; Mature: 42464

Theoretical pI: Translated: 4.47; Mature: 4.47

Prosite motif: PS50975 ATP_GRASP ; PS00843 DALA_DALA_LIGASE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDYINILQLSDNFTKNLQLTKNVNNYVDIVEKFRDKGEIMRIVVLAGGTSTERDVSLVTG
CCCEEEEEECCCCCCCEEEECCHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCHHHHHH
YRVCESLRRNGHEANMLDIFLGVDDSEVKTFFTEKNDLGELSDNLKKKTADIPAEVKRRT
HHHHHHHHHCCCCCCEEEEEEECCCHHHHHEECCCCCHHHHHHHHHHHHCCCCHHHHHHH
EARESFFGRNVLELCKEADMVFMGLHGSNGEDGKVQATFDLLGIRYTGTDYLSSAISMSK
HHHHHHHCCHHHHHHHCCCEEEEEECCCCCCCCEEEEEEEEEEEEECCHHHHHHHHHHHH
ELAKKVLVPEGIPMPKGVTLHKGHKIEYVPFPCVVKPCCGGSSVGVSIAHNEKEFEDALD
HHHHHHCCCCCCCCCCCEEEECCCEEEEECCCCEECCCCCCCCCEEEEECCHHHHHHHHH
EAFSYEDTVLVEEFVDGREFSVAVLDGKALPVIEIEPLEGFYDYKNKYKAGSTKETCPAN
HHHCCCCHHHHHHHCCCCEEEEEEECCCCCCEEEECCCCCHHHHHHHCCCCCCCCCCCCC
IPEDIASQMQFWAEKCCQAAGVTTYARVDELLDKNNNIFCLEINTLPGMTDTSLIPQEAA
CCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCHHHH
AVGISYDELTEKIIEISLAKYE
HCCCCHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MDYINILQLSDNFTKNLQLTKNVNNYVDIVEKFRDKGEIMRIVVLAGGTSTERDVSLVTG
CCCEEEEEECCCCCCCEEEECCHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCHHHHHH
YRVCESLRRNGHEANMLDIFLGVDDSEVKTFFTEKNDLGELSDNLKKKTADIPAEVKRRT
HHHHHHHHHCCCCCCEEEEEEECCCHHHHHEECCCCCHHHHHHHHHHHHCCCCHHHHHHH
EARESFFGRNVLELCKEADMVFMGLHGSNGEDGKVQATFDLLGIRYTGTDYLSSAISMSK
HHHHHHHCCHHHHHHHCCCEEEEEECCCCCCCCEEEEEEEEEEEEECCHHHHHHHHHHHH
ELAKKVLVPEGIPMPKGVTLHKGHKIEYVPFPCVVKPCCGGSSVGVSIAHNEKEFEDALD
HHHHHHCCCCCCCCCCCEEEECCCEEEEECCCCEECCCCCCCCCEEEEECCHHHHHHHHH
EAFSYEDTVLVEEFVDGREFSVAVLDGKALPVIEIEPLEGFYDYKNKYKAGSTKETCPAN
HHHCCCCHHHHHHHCCCCEEEEEEECCCCCCEEEECCCCCHHHHHHHCCCCCCCCCCCCC
IPEDIASQMQFWAEKCCQAAGVTTYARVDELLDKNNNIFCLEINTLPGMTDTSLIPQEAA
CCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCHHHH
AVGISYDELTEKIIEISLAKYE
HCCCCHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA