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The map label for this gene is argG

Identifier: 238916535

GI number: 238916535

Start: 603890

End: 605116

Strand: Reverse

Name: argG

Synonym: EUBELI_00592

Alternate gene names: 238916535

Gene position: 605116-603890 (Counterclockwise)

Preceding gene: 238916543

Following gene: 238916534

Centisome position: 28.22

GC content: 42.22

Gene sequence:

>1227_bases
ATGAAAGAAAAAGTTATTCTTGCTTATTCTGGCGGACTTGACACAACTGCCATCATCCCTTGGTTAAAGGAAACATACAA
CTATGATGTTGTCTGCGTATGTGCAGACTGTGGTCAGGAAGAGGAGTTGGACGGACTTGAGCAGAGAGCTCTCTCATGTG
GTGCTGCCAAGTTATATATAGAAGATATCACTGATGAGTTCTGCGACAACTACATCGTTCCATGTGTTCAGGCACACGCT
GTTTATGAAAATAAATATTTACTTGGTACTTCAATGGCAAGACCTCTTATTGCTAAGAGACTTGTTGAGATTGCCCGTAA
AGAAGGTGCTGTTGCTATCTGCCACGGTGCTACTGGTAAGGGTAATGACCAGATCAGATTCGAACTTACAATCAAGGCTT
TAGCTCCAGATATCAAGATTATCGCTCCTTGGAGAGATTCTAACTGGAAGCTTCAGTCTCGTGAGGATGAGATTGAATAC
TGCAGACAGCATGGTATTCATCTTCCATTCAGCACAGACTGCAGCTACAGCCGTGACCGTAACATCTGGCATATCAGCCA
TGAAGGTCTTGAGCTTGAAGATCCAGCTAACGAGCCTAACTACAAGCATTTATTAGTTCTTGGATGCACTCCTGAGGAAG
CTCCGGATGAACCAGAATATGTTACTATGACATTTGAAAAAGGTGTTCCAAAGTCAGTTAATGGCAAGGCTATGAAGGTT
TCTGATATTATCAGAGAGCTTAACAGACTTGGTGCCAAGCATGGTATCGGTATCATTGATATCGTTGAGAACCGTGTTGT
TGGTATGAAGTCCCGTGGTGTATATGAGACTCCTGGTGGAACAATTCTTTACGAAGCTCATCAGCAGCTTGAGGAATTAG
TTCTCGACAGAGATACAACAACATTCAAGATGGATGTTGGAAACAAGTTTGCACAGGTTGTTTACGAAGGCAAGTGGGAG
ACACCTCTTCGTGAAGCACTTCAGGCATTCGTTGAGAAGACTCAGGAATATGTAACAGGTGAAGTTAAGTTCAGACTTTA
CAAGGGTAACATCATCAAGGCTGGAACAACATCTCCATATTCACTTTACAATGAGTCAATTGCATCATTCAAGACAGGTG
ATATGTATGATCATCATGATGCTGACGGATTCATCAACCTCTTCGGTCTTTCACTTAAGGTAAGAGCTATGAAGAAGCTT
GAGAACGAGAAGAAGAACAATAAGTAA

Upstream 100 bases:

>100_bases
TTGTTGTTGCATTTTTAAATAAAGTAGTTTATTATAATCTGCAGGTGCATAGCATATGTGTTTTACTATGTAAACTTAAT
AATATACGGAGGAATATATA

Downstream 100 bases:

>100_bases
TTAAAGAATATAAAAAGCAGCATCCCCGGCATGGTTATTAATCATGTCGGGGATGTTGCTTTTATAATCCATTATGATTT
CCTATCAATTCATAAAAAAT

Product: argininosuccinate synthase

Products: NA

Alternate protein names: Citrulline--aspartate ligase

Number of amino acids: Translated: 408; Mature: 408

Protein sequence:

>408_residues
MKEKVILAYSGGLDTTAIIPWLKETYNYDVVCVCADCGQEEELDGLEQRALSCGAAKLYIEDITDEFCDNYIVPCVQAHA
VYENKYLLGTSMARPLIAKRLVEIARKEGAVAICHGATGKGNDQIRFELTIKALAPDIKIIAPWRDSNWKLQSREDEIEY
CRQHGIHLPFSTDCSYSRDRNIWHISHEGLELEDPANEPNYKHLLVLGCTPEEAPDEPEYVTMTFEKGVPKSVNGKAMKV
SDIIRELNRLGAKHGIGIIDIVENRVVGMKSRGVYETPGGTILYEAHQQLEELVLDRDTTTFKMDVGNKFAQVVYEGKWE
TPLREALQAFVEKTQEYVTGEVKFRLYKGNIIKAGTTSPYSLYNESIASFKTGDMYDHHDADGFINLFGLSLKVRAMKKL
ENEKKNNK

Sequences:

>Translated_408_residues
MKEKVILAYSGGLDTTAIIPWLKETYNYDVVCVCADCGQEEELDGLEQRALSCGAAKLYIEDITDEFCDNYIVPCVQAHA
VYENKYLLGTSMARPLIAKRLVEIARKEGAVAICHGATGKGNDQIRFELTIKALAPDIKIIAPWRDSNWKLQSREDEIEY
CRQHGIHLPFSTDCSYSRDRNIWHISHEGLELEDPANEPNYKHLLVLGCTPEEAPDEPEYVTMTFEKGVPKSVNGKAMKV
SDIIRELNRLGAKHGIGIIDIVENRVVGMKSRGVYETPGGTILYEAHQQLEELVLDRDTTTFKMDVGNKFAQVVYEGKWE
TPLREALQAFVEKTQEYVTGEVKFRLYKGNIIKAGTTSPYSLYNESIASFKTGDMYDHHDADGFINLFGLSLKVRAMKKL
ENEKKNNK
>Mature_408_residues
MKEKVILAYSGGLDTTAIIPWLKETYNYDVVCVCADCGQEEELDGLEQRALSCGAAKLYIEDITDEFCDNYIVPCVQAHA
VYENKYLLGTSMARPLIAKRLVEIARKEGAVAICHGATGKGNDQIRFELTIKALAPDIKIIAPWRDSNWKLQSREDEIEY
CRQHGIHLPFSTDCSYSRDRNIWHISHEGLELEDPANEPNYKHLLVLGCTPEEAPDEPEYVTMTFEKGVPKSVNGKAMKV
SDIIRELNRLGAKHGIGIIDIVENRVVGMKSRGVYETPGGTILYEAHQQLEELVLDRDTTTFKMDVGNKFAQVVYEGKWE
TPLREALQAFVEKTQEYVTGEVKFRLYKGNIIKAGTTSPYSLYNESIASFKTGDMYDHHDADGFINLFGLSLKVRAMKKL
ENEKKNNK

Specific function: Arginine biosynthesis; seventh step. [C]

COG id: COG0137

COG function: function code E; Argininosuccinate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the argininosuccinate synthase family. Type 1 subfamily

Homologues:

Organism=Homo sapiens, GI53759107, Length=399, Percent_Identity=46.3659147869674, Blast_Score=349, Evalue=2e-96,
Organism=Homo sapiens, GI16950633, Length=399, Percent_Identity=46.3659147869674, Blast_Score=349, Evalue=2e-96,
Organism=Escherichia coli, GI1789563, Length=366, Percent_Identity=29.5081967213115, Blast_Score=149, Evalue=3e-37,
Organism=Saccharomyces cerevisiae, GI6324514, Length=405, Percent_Identity=39.7530864197531, Blast_Score=283, Evalue=3e-77,
Organism=Drosophila melanogaster, GI21358151, Length=419, Percent_Identity=43.9140811455847, Blast_Score=326, Evalue=2e-89,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): ASSY_EUBE2 (C4Z4C1)

Other databases:

- EMBL:   CP001104
- RefSeq:   YP_002930052.1
- GeneID:   7958651
- GenomeReviews:   CP001104_GR
- KEGG:   eel:EUBELI_00592
- OMA:   VAVDVGQ
- ProtClustDB:   PRK00509
- GO:   GO:0005737
- HAMAP:   MF_00005
- InterPro:   IPR001518
- InterPro:   IPR018223
- InterPro:   IPR014729
- Gene3D:   G3DSA:3.40.50.620
- PANTHER:   PTHR11587
- TIGRFAMs:   TIGR00032

Pfam domain/function: PF00764 Arginosuc_synth

EC number: =6.3.4.5

Molecular weight: Translated: 46073; Mature: 46073

Theoretical pI: Translated: 5.37; Mature: 5.37

Prosite motif: PS00564 ARGININOSUCCIN_SYN_1; PS00565 ARGININOSUCCIN_SYN_2

Important sites: BINDING 35-35 BINDING 86-86 BINDING 91-91 BINDING 116-116 BINDING 118-118 BINDING 122-122 BINDING 122-122 BINDING 123-123 BINDING 126-126 BINDING 177-177 BINDING 186-186 BINDING 263-263 BINDING 275-275

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKEKVILAYSGGLDTTAIIPWLKETYNYDVVCVCADCGQEEELDGLEQRALSCGAAKLYI
CCCCEEEEECCCCCCHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHCCHHEEEH
EDITDEFCDNYIVPCVQAHAVYENKYLLGTSMARPLIAKRLVEIARKEGAVAICHGATGK
HHHHHHHHCCCCCCHHHHHEEECCCEEECCHHHHHHHHHHHHHHHHHCCCEEEEECCCCC
GNDQIRFELTIKALAPDIKIIAPWRDSNWKLQSREDEIEYCRQHGIHLPFSTDCSYSRDR
CCCEEEEEEEEEEECCCEEEEECCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCC
NIWHISHEGLELEDPANEPNYKHLLVLGCTPEEAPDEPEYVTMTFEKGVPKSVNGKAMKV
CEEEEECCCEECCCCCCCCCCEEEEEEECCCCCCCCCCCEEEEEECCCCCCCCCCCEEEH
SDIIRELNRLGAKHGIGIIDIVENRVVGMKSRGVYETPGGTILYEAHQQLEELVLDRDTT
HHHHHHHHHCCHHHCCEEEEEECCCEECCCCCCCEECCCCEEEEHHHHHHHHHHHCCCCC
TFKMDVGNKFAQVVYEGKWETPLREALQAFVEKTQEYVTGEVKFRLYKGNIIKAGTTSPY
EEEEECCCCEEHHEECCCCCCHHHHHHHHHHHHHHHHEEEEEEEEEEECCEEECCCCCCH
SLYNESIASFKTGDMYDHHDADGFINLFGLSLKVRAMKKLENEKKNNK
HHHHHHHHCCCCCCCCCCCCCCCEEEEEECEEHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MKEKVILAYSGGLDTTAIIPWLKETYNYDVVCVCADCGQEEELDGLEQRALSCGAAKLYI
CCCCEEEEECCCCCCHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHCCHHEEEH
EDITDEFCDNYIVPCVQAHAVYENKYLLGTSMARPLIAKRLVEIARKEGAVAICHGATGK
HHHHHHHHCCCCCCHHHHHEEECCCEEECCHHHHHHHHHHHHHHHHHCCCEEEEECCCCC
GNDQIRFELTIKALAPDIKIIAPWRDSNWKLQSREDEIEYCRQHGIHLPFSTDCSYSRDR
CCCEEEEEEEEEEECCCEEEEECCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCC
NIWHISHEGLELEDPANEPNYKHLLVLGCTPEEAPDEPEYVTMTFEKGVPKSVNGKAMKV
CEEEEECCCEECCCCCCCCCCEEEEEEECCCCCCCCCCCEEEEEECCCCCCCCCCCEEEH
SDIIRELNRLGAKHGIGIIDIVENRVVGMKSRGVYETPGGTILYEAHQQLEELVLDRDTT
HHHHHHHHHCCHHHCCEEEEEECCCEECCCCCCCEECCCCEEEEHHHHHHHHHHHCCCCC
TFKMDVGNKFAQVVYEGKWETPLREALQAFVEKTQEYVTGEVKFRLYKGNIIKAGTTSPY
EEEEECCCCEEHHEECCCCCCHHHHHHHHHHHHHHHHEEEEEEEEEEECCEEECCCCCCH
SLYNESIASFKTGDMYDHHDADGFINLFGLSLKVRAMKKLENEKKNNK
HHHHHHHHCCCCCCCCCCCCCCCEEEEEECEEHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA