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The map label for this gene is groL

Identifier: 238916463

GI number: 238916463

Start: 525501

End: 527126

Strand: Direct

Name: groL

Synonym: EUBELI_00520

Alternate gene names: 238916463

Gene position: 525501-527126 (Clockwise)

Preceding gene: 238916462

Following gene: 238916464

Centisome position: 24.51

GC content: 41.7

Gene sequence:

>1626_bases
ATGGCAAAGGAAATCAAGTACGGAATTGAAGCTAGAAAGGCTTTAGAGGAAGGTGTTAATAAGTTAGCTAATACAGTAAG
AGTAACTATCGGACCTAAGGGTCGTAATGTTGTTCTTGATAAGTCATATGGTGCACCACTTATCACTAACGATGGTGTTA
CTATTGCTAAGGATATTGAACTTGAAGATGCATTCGAGAATATGGGTGCACAGCTTGTTAAGGAAGTAGCAGCCAAGACT
AATGATGTTGCAGGTGATGGTACAACAACAGCTACTGTTCTTGCACAGGCTATGATTAATGAAGGTATGAAGAACCTTGC
AGCAGGTGCTAACCCAATCGTATTAAGAAAGGGAATGAAGAAGGCTACTGATTGTGCTGTTGAAGCTATTGCAGGTATGA
GCGAGAAGGTAACAGGCAAGGATCAGATTGCCAAGGTTGCAGCTATTTCAGCAGGTGATGAGGAAGTTGGACAGATGGTA
GCTGATGCTATGGAGAAGGTTTCTAACGATGGCGTTATCACAATCGAGGAATCTAAGACAATGAAGACAGAGCTTGACCT
TGTAGAAGGTATGCAGTTCGATAGAGGATACATTTCAGCATATATGGCTACAGATATGGATAAGATGGAAGCAGTTCTTG
ATAATCCATATATCCTTATTACAGATAAGAAGATTTCTAATATTCAGGAAATTTTACCAGTTCTTGAGCAGATTGTTCAG
AGTGGTGCCAAGCTTCTTATTATTGCAGAAGATGTTGAGGGCGAGGCTCTTACAACACTTATCGTTAATAAGTTAAGAGG
AACATTCAATGTTGTAGCTGTCAAGGCTCCTGGATATGGTGACAGACGTAAGGAAATGCTTAAGGATATCGCAATTCTTA
CAGGTGGTCAGGTTATTTCTGAGGAACTTGGACTTGAGCTTAAGGATACAACAATGGATATGCTTGGTAGAGCTAAGTCT
GTTAAGGTTCAGAAAGAGAACACAGTTATCGTTGATGGCGAAGGTGCTAAGGAAGATATTGATGCAAGAGTTGCACAGAT
TAAGGCACAGCTTGAGGAGACAACTTCTGAATTTGATAAAGAGAAGTTACAGGAAAGACTTGCAAAGCTTGCAGGTGGTG
TAGCTGTTATCAGAGTTGGTGCTGCAACAGAGACAGAGATGAAGGAAGCTAAGCTTCGTATGGAAGATGCTCTTAATGCA
ACAAGAGCTGCTGTTGAAGAAGGTGTAGTATCAGGTGGTGGTTCTGCTTATATCCATGCTTCTAAGAAGGTTGCAGAGCT
TGTTAAGACACTCAGTGGGGATGAGAAGATTGGTGCCCAGATTATCTTAAAAGCACTTGAAGCTCCATTATTCCACATTG
CATACAATGCTGGTCTTGAAGGCGCTGTTATCATCAATAAGGTAAGAGAGTCAGAAGTTGGTACAGGATTTGATGCATAC
AAGGAAGAGTATGTCAACATGATTGACGCTGGTATCCTTGACCCGGCTAAGGTTACAAGAAGTGCATTACAGAATGCTAC
AAGCGTTGCTTCAACACTTCTTACAACAGAGTCAGTTGTTGCTAATATTAAGGAAGACGCTCCAGCAATGCCAGCAGGCG
GTGCAGGCATGGGCGGAATGATGTAA

Upstream 100 bases:

>100_bases
AGTCTGATATTCTTGCTATCATTGAATAATTGACAATGTCAGTTGCAGATGAGGGCGGTATGAACATATAAGAAGTATAT
TTATTTAGGAGGCTATTATT

Downstream 100 bases:

>100_bases
TTCCACAATATAACTAAAACATAGAGCCCGGTGGGTAAATTCCCGCCGGGCATATTGCTTTAAAGAAAGGTTATTGAATT
ATGATAGAAGGAACAGTAGA

Product: chaperonin GroEL

Products: NA

Alternate protein names: GroEL protein; Protein Cpn60

Number of amino acids: Translated: 541; Mature: 540

Protein sequence:

>541_residues
MAKEIKYGIEARKALEEGVNKLANTVRVTIGPKGRNVVLDKSYGAPLITNDGVTIAKDIELEDAFENMGAQLVKEVAAKT
NDVAGDGTTTATVLAQAMINEGMKNLAAGANPIVLRKGMKKATDCAVEAIAGMSEKVTGKDQIAKVAAISAGDEEVGQMV
ADAMEKVSNDGVITIEESKTMKTELDLVEGMQFDRGYISAYMATDMDKMEAVLDNPYILITDKKISNIQEILPVLEQIVQ
SGAKLLIIAEDVEGEALTTLIVNKLRGTFNVVAVKAPGYGDRRKEMLKDIAILTGGQVISEELGLELKDTTMDMLGRAKS
VKVQKENTVIVDGEGAKEDIDARVAQIKAQLEETTSEFDKEKLQERLAKLAGGVAVIRVGAATETEMKEAKLRMEDALNA
TRAAVEEGVVSGGGSAYIHASKKVAELVKTLSGDEKIGAQIILKALEAPLFHIAYNAGLEGAVIINKVRESEVGTGFDAY
KEEYVNMIDAGILDPAKVTRSALQNATSVASTLLTTESVVANIKEDAPAMPAGGAGMGGMM

Sequences:

>Translated_541_residues
MAKEIKYGIEARKALEEGVNKLANTVRVTIGPKGRNVVLDKSYGAPLITNDGVTIAKDIELEDAFENMGAQLVKEVAAKT
NDVAGDGTTTATVLAQAMINEGMKNLAAGANPIVLRKGMKKATDCAVEAIAGMSEKVTGKDQIAKVAAISAGDEEVGQMV
ADAMEKVSNDGVITIEESKTMKTELDLVEGMQFDRGYISAYMATDMDKMEAVLDNPYILITDKKISNIQEILPVLEQIVQ
SGAKLLIIAEDVEGEALTTLIVNKLRGTFNVVAVKAPGYGDRRKEMLKDIAILTGGQVISEELGLELKDTTMDMLGRAKS
VKVQKENTVIVDGEGAKEDIDARVAQIKAQLEETTSEFDKEKLQERLAKLAGGVAVIRVGAATETEMKEAKLRMEDALNA
TRAAVEEGVVSGGGSAYIHASKKVAELVKTLSGDEKIGAQIILKALEAPLFHIAYNAGLEGAVIINKVRESEVGTGFDAY
KEEYVNMIDAGILDPAKVTRSALQNATSVASTLLTTESVVANIKEDAPAMPAGGAGMGGMM
>Mature_540_residues
AKEIKYGIEARKALEEGVNKLANTVRVTIGPKGRNVVLDKSYGAPLITNDGVTIAKDIELEDAFENMGAQLVKEVAAKTN
DVAGDGTTTATVLAQAMINEGMKNLAAGANPIVLRKGMKKATDCAVEAIAGMSEKVTGKDQIAKVAAISAGDEEVGQMVA
DAMEKVSNDGVITIEESKTMKTELDLVEGMQFDRGYISAYMATDMDKMEAVLDNPYILITDKKISNIQEILPVLEQIVQS
GAKLLIIAEDVEGEALTTLIVNKLRGTFNVVAVKAPGYGDRRKEMLKDIAILTGGQVISEELGLELKDTTMDMLGRAKSV
KVQKENTVIVDGEGAKEDIDARVAQIKAQLEETTSEFDKEKLQERLAKLAGGVAVIRVGAATETEMKEAKLRMEDALNAT
RAAVEEGVVSGGGSAYIHASKKVAELVKTLSGDEKIGAQIILKALEAPLFHIAYNAGLEGAVIINKVRESEVGTGFDAYK
EEYVNMIDAGILDPAKVTRSALQNATSVASTLLTTESVVANIKEDAPAMPAGGAGMGGMM

Specific function: Prevents misfolding and promotes the refolding and proper assembly of unfolded polypeptides generated under stress conditions

COG id: COG0459

COG function: function code O; Chaperonin GroEL (HSP60 family)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the chaperonin (HSP60) family

Homologues:

Organism=Homo sapiens, GI41399285, Length=527, Percent_Identity=48.1973434535104, Blast_Score=482, Evalue=1e-136,
Organism=Homo sapiens, GI31542947, Length=527, Percent_Identity=48.1973434535104, Blast_Score=482, Evalue=1e-136,
Organism=Homo sapiens, GI38455427, Length=547, Percent_Identity=23.400365630713, Blast_Score=93, Evalue=7e-19,
Organism=Homo sapiens, GI5453607, Length=542, Percent_Identity=23.6162361623616, Blast_Score=90, Evalue=7e-18,
Organism=Homo sapiens, GI261399877, Length=520, Percent_Identity=23.2692307692308, Blast_Score=76, Evalue=9e-14,
Organism=Homo sapiens, GI24307939, Length=504, Percent_Identity=23.015873015873, Blast_Score=71, Evalue=3e-12,
Organism=Homo sapiens, GI261399875, Length=479, Percent_Identity=22.5469728601253, Blast_Score=68, Evalue=3e-11,
Organism=Escherichia coli, GI1790586, Length=528, Percent_Identity=58.7121212121212, Blast_Score=596, Evalue=1e-172,
Organism=Caenorhabditis elegans, GI17555558, Length=530, Percent_Identity=47.1698113207547, Blast_Score=467, Evalue=1e-132,
Organism=Caenorhabditis elegans, GI193210679, Length=212, Percent_Identity=46.2264150943396, Blast_Score=174, Evalue=1e-43,
Organism=Caenorhabditis elegans, GI17564182, Length=547, Percent_Identity=23.400365630713, Blast_Score=98, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI17532603, Length=539, Percent_Identity=21.3358070500928, Blast_Score=74, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI25144674, Length=187, Percent_Identity=30.4812834224599, Blast_Score=71, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6323288, Length=525, Percent_Identity=52.3809523809524, Blast_Score=518, Evalue=1e-148,
Organism=Saccharomyces cerevisiae, GI6322524, Length=580, Percent_Identity=21.551724137931, Blast_Score=79, Evalue=1e-15,
Organism=Drosophila melanogaster, GI24641193, Length=528, Percent_Identity=48.6742424242424, Blast_Score=493, Evalue=1e-139,
Organism=Drosophila melanogaster, GI24641191, Length=528, Percent_Identity=48.6742424242424, Blast_Score=493, Evalue=1e-139,
Organism=Drosophila melanogaster, GI45550936, Length=524, Percent_Identity=45.9923664122137, Blast_Score=459, Evalue=1e-129,
Organism=Drosophila melanogaster, GI45550132, Length=524, Percent_Identity=45.9923664122137, Blast_Score=459, Evalue=1e-129,
Organism=Drosophila melanogaster, GI45550935, Length=524, Percent_Identity=45.9923664122137, Blast_Score=459, Evalue=1e-129,
Organism=Drosophila melanogaster, GI17864606, Length=542, Percent_Identity=39.2988929889299, Blast_Score=397, Evalue=1e-111,
Organism=Drosophila melanogaster, GI24584129, Length=534, Percent_Identity=34.4569288389513, Blast_Score=299, Evalue=4e-81,
Organism=Drosophila melanogaster, GI19921262, Length=534, Percent_Identity=34.4569288389513, Blast_Score=299, Evalue=4e-81,
Organism=Drosophila melanogaster, GI24645179, Length=515, Percent_Identity=23.3009708737864, Blast_Score=91, Evalue=2e-18,
Organism=Drosophila melanogaster, GI24583944, Length=543, Percent_Identity=22.6519337016575, Blast_Score=75, Evalue=2e-13,
Organism=Drosophila melanogaster, GI17647245, Length=508, Percent_Identity=22.8346456692913, Blast_Score=74, Evalue=4e-13,
Organism=Drosophila melanogaster, GI24652903, Length=496, Percent_Identity=22.7822580645161, Blast_Score=73, Evalue=6e-13,

Paralogues:

None

Copy number: 2180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 360 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 480 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 15012 Molecules/Cell In: Growth Phase,

Swissprot (AC and ID): CH60_EUBE2 (C4Z3R4)

Other databases:

- EMBL:   CP001104
- RefSeq:   YP_002929980.1
- GeneID:   7958579
- GenomeReviews:   CP001104_GR
- KEGG:   eel:EUBELI_00520
- OMA:   DVKFHDS
- ProtClustDB:   CLSK2541017
- GO:   GO:0005737
- HAMAP:   MF_00600
- InterPro:   IPR018370
- InterPro:   IPR001844
- InterPro:   IPR002423
- PANTHER:   PTHR11353
- PRINTS:   PR00298
- TIGRFAMs:   TIGR02348

Pfam domain/function: PF00118 Cpn60_TCP1; SSF48592 GroEL-ATPase

EC number: NA

Molecular weight: Translated: 57397; Mature: 57266

Theoretical pI: Translated: 4.57; Mature: 4.57

Prosite motif: PS00296 CHAPERONINS_CPN60

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKEIKYGIEARKALEEGVNKLANTVRVTIGPKGRNVVLDKSYGAPLITNDGVTIAKDIE
CCCCHHHHHHHHHHHHHHHHHHHHEEEEEECCCCCEEEEECCCCCCEEECCCEEEEECCC
LEDAFENMGAQLVKEVAAKTNDVAGDGTTTATVLAQAMINEGMKNLAAGANPIVLRKGMK
HHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCH
KATDCAVEAIAGMSEKVTGKDQIAKVAAISAGDEEVGQMVADAMEKVSNDGVITIEESKT
HHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCEEEEECCCH
MKTELDLVEGMQFDRGYISAYMATDMDKMEAVLDNPYILITDKKISNIQEILPVLEQIVQ
HHHHHHHHHCCCCCCHHHHHHHHCCHHHHHHHHCCCEEEEECCHHHHHHHHHHHHHHHHH
SGAKLLIIAEDVEGEALTTLIVNKLRGTFNVVAVKAPGYGDRRKEMLKDIAILTGGQVIS
CCCEEEEEEECCCCHHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHHHHHCCCHHHH
EELGLELKDTTMDMLGRAKSVKVQKENTVIVDGEGAKEDIDARVAQIKAQLEETTSEFDK
HHHCCEEHHHHHHHHCCHHCEEEEECCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHH
EKLQERLAKLAGGVAVIRVGAATETEMKEAKLRMEDALNATRAAVEEGVVSGGGSAYIHA
HHHHHHHHHHHCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEC
SKKVAELVKTLSGDEKIGAQIILKALEAPLFHIAYNAGLEGAVIINKVRESEVGTGFDAY
HHHHHHHHHHHCCCHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHCCCCCHHHH
KEEYVNMIDAGILDPAKVTRSALQNATSVASTLLTTESVVANIKEDAPAMPAGGAGMGGM
HHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC
M
C
>Mature Secondary Structure 
AKEIKYGIEARKALEEGVNKLANTVRVTIGPKGRNVVLDKSYGAPLITNDGVTIAKDIE
CCCHHHHHHHHHHHHHHHHHHHHEEEEEECCCCCEEEEECCCCCCEEECCCEEEEECCC
LEDAFENMGAQLVKEVAAKTNDVAGDGTTTATVLAQAMINEGMKNLAAGANPIVLRKGMK
HHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCH
KATDCAVEAIAGMSEKVTGKDQIAKVAAISAGDEEVGQMVADAMEKVSNDGVITIEESKT
HHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCEEEEECCCH
MKTELDLVEGMQFDRGYISAYMATDMDKMEAVLDNPYILITDKKISNIQEILPVLEQIVQ
HHHHHHHHHCCCCCCHHHHHHHHCCHHHHHHHHCCCEEEEECCHHHHHHHHHHHHHHHHH
SGAKLLIIAEDVEGEALTTLIVNKLRGTFNVVAVKAPGYGDRRKEMLKDIAILTGGQVIS
CCCEEEEEEECCCCHHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHHHHHCCCHHHH
EELGLELKDTTMDMLGRAKSVKVQKENTVIVDGEGAKEDIDARVAQIKAQLEETTSEFDK
HHHCCEEHHHHHHHHCCHHCEEEEECCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHH
EKLQERLAKLAGGVAVIRVGAATETEMKEAKLRMEDALNATRAAVEEGVVSGGGSAYIHA
HHHHHHHHHHHCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEC
SKKVAELVKTLSGDEKIGAQIILKALEAPLFHIAYNAGLEGAVIINKVRESEVGTGFDAY
HHHHHHHHHHHCCCHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHCCCCCHHHH
KEEYVNMIDAGILDPAKVTRSALQNATSVASTLLTTESVVANIKEDAPAMPAGGAGMGGM
HHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC
M
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA