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The map label for this gene is purA [H]

Identifier: 238916438

GI number: 238916438

Start: 501383

End: 502678

Strand: Direct

Name: purA [H]

Synonym: EUBELI_00495

Alternate gene names: 238916438

Gene position: 501383-502678 (Clockwise)

Preceding gene: 238916437

Following gene: 238916439

Centisome position: 23.38

GC content: 39.66

Gene sequence:

>1296_bases
ATGGTAAAAGCTATTGTAGGTGCTAACTGGGGTGATGAAGGAAAGGGTAAGATTACTGATATGCTTGCCGAAGAATCAGA
CATCATCGTAAGATTCCAGGGAGGAGCTAATGCGGGACATACTATTATTAATGATTATGGAAAGTTCGCGCTCCATCTTT
TACCTTCGGGAGTTTTCTATAACCATACAACAAGTGTCATTGGCAACGGTGTAGCTCTTAATATTCCATATCTTGTTAAG
GAATTAAAGAGCCTTACAGACCGCAATGTACCAATGCCTAAGATTCTTGTATCAGACAGGGCACAGATTCTTATGCCTTA
TCATGTTGCATTTGATACATATGAAGAAGCAAGACTTGCTGGTAAGTCATTTGGTTCTACTAAGTCAGGTATTGCTCCAT
TCTATTCAGATAAGTATGCTAAGATTGGTTTCCAGGTTAATGAATTATTTGGAGACGAGCAGGAACTTAAGGAAAAGATT
GCTAATGTATGTACATTAAAGAATGTTATGCTTGAGCATCTCTATCATCAGCCACTTCTTAACGAAGAAGAGATATTCAA
TACTCTTATGGAATATAAAGAGATGGTTGAGCCATATGTATGTGATACATCAGCATTTTTACATCAGGCACTTAAAGACG
GAAAGAAGATCTTACTTGAGGGTCAGTTAGGCTCTCTTAAGGATACTGACCATGGTATATACCCAATGGTAACTTCATCA
TCAACATTAGCTCCTTATGGTGCTATTGGTGCTGGTATTCCGGCTGCATCAATTACTGATGTTGTTACAGTTGTTAAGGC
ATATTCTTCAGCAGTTGGTGCCGGAGCATTTGTAAGTGAAATCTTTGGTGATGAGGCAGATGAACTCAGACGCAGAGGTG
GAGACGGTGGTGAATTCGGAGCTACTACAGGACGTCCTAGAAGAATGGGATGGTTCGATGCAGTTGCTACACGTTATGGT
GTAAGAATGCAGGGAACAACAGAGGTTGCTCTTACAGTTCTTGATGTACTTGGATATCTTGATGAGATTCCAATGTGCGT
TGGATATGAGATAGATGGAAAGATTACTAAGGATTTCCCAACTACTCCACAGCTTGAGAAGGCTAAGCCGGTATTAAAGA
CTATGCCAGGCTGGAAGTCAGATATCAGAGGAATTACTAACTATGAAGAACTTCCTGTTGAGTGTCGTAACTATATTGAA
ACTATTGAGCAGGAGATTGAAGCTCCTATCACAATGGTATCTAATGGTCCAGGAAGACATGAGATTATCCACAGAAAGAG
TAATTTTTCTAAATAA

Upstream 100 bases:

>100_bases
GGGGTAGAGCACTTCATTGGTAATGAAGAGGTCACGGGTTCAATTCCCGTCAATAGCTTAATTTAATGTTTGTGTTTTTT
AAAACAGATTGGAGGACGCT

Downstream 100 bases:

>100_bases
TAACAATTAATATTACCGTATCTGCAGTACAACAGTTTTGCAGATACGGTCTTTTTATAGGTAATTGGAGGAACATATGA
AGTTTAATAAATATTTTGAC

Product: adenylosuccinate synthase

Products: NA

Alternate protein names: AMPSase; AdSS; IMP--aspartate ligase [H]

Number of amino acids: Translated: 431; Mature: 431

Protein sequence:

>431_residues
MVKAIVGANWGDEGKGKITDMLAEESDIIVRFQGGANAGHTIINDYGKFALHLLPSGVFYNHTTSVIGNGVALNIPYLVK
ELKSLTDRNVPMPKILVSDRAQILMPYHVAFDTYEEARLAGKSFGSTKSGIAPFYSDKYAKIGFQVNELFGDEQELKEKI
ANVCTLKNVMLEHLYHQPLLNEEEIFNTLMEYKEMVEPYVCDTSAFLHQALKDGKKILLEGQLGSLKDTDHGIYPMVTSS
STLAPYGAIGAGIPAASITDVVTVVKAYSSAVGAGAFVSEIFGDEADELRRRGGDGGEFGATTGRPRRMGWFDAVATRYG
VRMQGTTEVALTVLDVLGYLDEIPMCVGYEIDGKITKDFPTTPQLEKAKPVLKTMPGWKSDIRGITNYEELPVECRNYIE
TIEQEIEAPITMVSNGPGRHEIIHRKSNFSK

Sequences:

>Translated_431_residues
MVKAIVGANWGDEGKGKITDMLAEESDIIVRFQGGANAGHTIINDYGKFALHLLPSGVFYNHTTSVIGNGVALNIPYLVK
ELKSLTDRNVPMPKILVSDRAQILMPYHVAFDTYEEARLAGKSFGSTKSGIAPFYSDKYAKIGFQVNELFGDEQELKEKI
ANVCTLKNVMLEHLYHQPLLNEEEIFNTLMEYKEMVEPYVCDTSAFLHQALKDGKKILLEGQLGSLKDTDHGIYPMVTSS
STLAPYGAIGAGIPAASITDVVTVVKAYSSAVGAGAFVSEIFGDEADELRRRGGDGGEFGATTGRPRRMGWFDAVATRYG
VRMQGTTEVALTVLDVLGYLDEIPMCVGYEIDGKITKDFPTTPQLEKAKPVLKTMPGWKSDIRGITNYEELPVECRNYIE
TIEQEIEAPITMVSNGPGRHEIIHRKSNFSK
>Mature_431_residues
MVKAIVGANWGDEGKGKITDMLAEESDIIVRFQGGANAGHTIINDYGKFALHLLPSGVFYNHTTSVIGNGVALNIPYLVK
ELKSLTDRNVPMPKILVSDRAQILMPYHVAFDTYEEARLAGKSFGSTKSGIAPFYSDKYAKIGFQVNELFGDEQELKEKI
ANVCTLKNVMLEHLYHQPLLNEEEIFNTLMEYKEMVEPYVCDTSAFLHQALKDGKKILLEGQLGSLKDTDHGIYPMVTSS
STLAPYGAIGAGIPAASITDVVTVVKAYSSAVGAGAFVSEIFGDEADELRRRGGDGGEFGATTGRPRRMGWFDAVATRYG
VRMQGTTEVALTVLDVLGYLDEIPMCVGYEIDGKITKDFPTTPQLEKAKPVLKTMPGWKSDIRGITNYEELPVECRNYIE
TIEQEIEAPITMVSNGPGRHEIIHRKSNFSK

Specific function: Plays an important role in the de novo pathway of purine nucleotide biosynthesis. Catalyzes the first commited step in the biosynthesis of AMP from IMP [H]

COG id: COG0104

COG function: function code F; Adenylosuccinate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the adenylosuccinate synthetase family [H]

Homologues:

Organism=Homo sapiens, GI34577063, Length=432, Percent_Identity=38.6574074074074, Blast_Score=291, Evalue=8e-79,
Organism=Homo sapiens, GI22748717, Length=430, Percent_Identity=38.1395348837209, Blast_Score=288, Evalue=9e-78,
Organism=Homo sapiens, GI40316944, Length=402, Percent_Identity=36.81592039801, Blast_Score=245, Evalue=6e-65,
Organism=Escherichia coli, GI1790620, Length=431, Percent_Identity=39.6751740139211, Blast_Score=316, Evalue=2e-87,
Organism=Caenorhabditis elegans, GI25146555, Length=437, Percent_Identity=35.9267734553776, Blast_Score=259, Evalue=1e-69,
Organism=Caenorhabditis elegans, GI25146553, Length=436, Percent_Identity=36.0091743119266, Blast_Score=259, Evalue=2e-69,
Organism=Saccharomyces cerevisiae, GI6324109, Length=437, Percent_Identity=37.7574370709382, Blast_Score=286, Evalue=5e-78,
Organism=Drosophila melanogaster, GI21356033, Length=435, Percent_Identity=39.5402298850575, Blast_Score=296, Evalue=2e-80,

Paralogues:

None

Copy number: 940 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018220
- InterPro:   IPR001114 [H]

Pfam domain/function: PF00709 Adenylsucc_synt [H]

EC number: =6.3.4.4 [H]

Molecular weight: Translated: 47379; Mature: 47379

Theoretical pI: Translated: 5.22; Mature: 5.22

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVKAIVGANWGDEGKGKITDMLAEESDIIVRFQGGANAGHTIINDYGKFALHLLPSGVFY
CCEEEECCCCCCCCCCHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHCCCCEE
NHTTSVIGNGVALNIPYLVKELKSLTDRNVPMPKILVSDRAQILMPYHVAFDTYEEARLA
CCHHHHHCCCEEEECHHHHHHHHHHHCCCCCCCHHHHCCCCCEEEEHHHHHCCHHHHHHH
GKSFGSTKSGIAPFYSDKYAKIGFQVNELFGDEQELKEKIANVCTLKNVMLEHLYHQPLL
HCCCCCCCCCCCCCCCCCHHHCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCC
NEEEIFNTLMEYKEMVEPYVCDTSAFLHQALKDGKKILLEGQLGSLKDTDHGIYPMVTSS
CHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCEEEECC
STLAPYGAIGAGIPAASITDVVTVVKAYSSAVGAGAFVSEIFGDEADELRRRGGDGGEFG
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCHHHHHHHHHCCCHHHHHHCCCCCCCCC
ATTGRPRRMGWFDAVATRYGVRMQGTTEVALTVLDVLGYLDEIPMCVGYEIDGKITKDFP
CCCCCCCCCHHHHHHHHHHCCEECCHHHHHHHHHHHHHHHHHCCCCCCEEECCEEECCCC
TTPQLEKAKPVLKTMPGWKSDIRGITNYEELPVECRNYIETIEQEIEAPITMVSNGPGRH
CCCCHHHCCHHHHHCCCCHHHHCCCCCHHHCCHHHHHHHHHHHHHHCCCEEEECCCCCHH
EIIHRKSNFSK
HHHCCCCCCCC
>Mature Secondary Structure
MVKAIVGANWGDEGKGKITDMLAEESDIIVRFQGGANAGHTIINDYGKFALHLLPSGVFY
CCEEEECCCCCCCCCCHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHCCCCEE
NHTTSVIGNGVALNIPYLVKELKSLTDRNVPMPKILVSDRAQILMPYHVAFDTYEEARLA
CCHHHHHCCCEEEECHHHHHHHHHHHCCCCCCCHHHHCCCCCEEEEHHHHHCCHHHHHHH
GKSFGSTKSGIAPFYSDKYAKIGFQVNELFGDEQELKEKIANVCTLKNVMLEHLYHQPLL
HCCCCCCCCCCCCCCCCCHHHCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCC
NEEEIFNTLMEYKEMVEPYVCDTSAFLHQALKDGKKILLEGQLGSLKDTDHGIYPMVTSS
CHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCEEEECC
STLAPYGAIGAGIPAASITDVVTVVKAYSSAVGAGAFVSEIFGDEADELRRRGGDGGEFG
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCHHHHHHHHHCCCHHHHHHCCCCCCCCC
ATTGRPRRMGWFDAVATRYGVRMQGTTEVALTVLDVLGYLDEIPMCVGYEIDGKITKDFP
CCCCCCCCCHHHHHHHHHHCCEECCHHHHHHHHHHHHHHHHHCCCCCCEEECCEEECCCC
TTPQLEKAKPVLKTMPGWKSDIRGITNYEELPVECRNYIETIEQEIEAPITMVSNGPGRH
CCCCHHHCCHHHHHCCCCHHHHCCCCCHHHCCHHHHHHHHHHHHHHCCCEEEECCCCCHH
EIIHRKSNFSK
HHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA