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The map label for this gene is lrgB [H]

Identifier: 238916383

GI number: 238916383

Start: 449018

End: 449710

Strand: Direct

Name: lrgB [H]

Synonym: EUBELI_00437

Alternate gene names: 238916383

Gene position: 449018-449710 (Clockwise)

Preceding gene: 238916382

Following gene: 238916384

Centisome position: 20.94

GC content: 36.65

Gene sequence:

>693_bases
ATGAATGATTTTTTCAGAGAATCACTTTTTGCCGGAGTCACATTAAGTCTTTTATCATACTTTATTGGTTCTGTGCTTAA
GAAAAAATTTAAGCTTGGAATATTCAATCCACTGCTTATATCAATAATAATAACAATAATAGTACTTGCAGTATCAGGCG
TTGATTATGATGTATATAATCAGGGTGCCAGGTATCTTAGCTGGTTTCTTACACCGGCAACTGTATGTCTTGCAATTCCG
CTGTATGAGCAGTGGAGTCTTCTTAAGAAGAATTACAAGGCAGTTGTTGTAGGAATAGCATCAGGTGTGCTTACAAGTTT
AACAACGGTACTTGTGTTATCTAAAATTATGAATCTGAGTCATGCAGAATATGTTACTCTTCTTCCGAAGTCAATAACTA
CTGCTATTGGTATGGGAGTTTCTGAGGAACTTGGAGGTTATGTAACAATCACAGTTGCAGTTATTGTTGTAACAGGAGTT
CTTGGAAATATATTTGGTGAGCTTATATGTAAAATATTCAGAATAAAAGAACCGATTGCCAAAGGACTTGCGTTAGGATC
TGCTGCACATGCGATAGGAACAGCCAAGGCTATGGAAATGGGCGAAGTTGAAGGTGCAATGAGCAGCTTATCAATTGCAG
TTGCAGGTATACTTACAGTTGTACTTTCATCAGTATTTGCTGGATTTATGTAA

Upstream 100 bases:

>100_bases
ATTACGGTTATGGCGGTTACAGGTCAGGCTTCACAGATAATCATACGTGCTGACAGGAAGAGAGAATTACGCAAAGCACA
AAGAAATGAGGTTAATACTA

Downstream 100 bases:

>100_bases
AATTAGGAGGCGTTAAACATGATAATCACAATTGCAAGACAATGTGGATGTATGGGAGACCAGATAGGAAAAGCACTGGC
AGAGAAATATTTTATACCAT

Product: holin-like protein LrgB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 230; Mature: 230

Protein sequence:

>230_residues
MNDFFRESLFAGVTLSLLSYFIGSVLKKKFKLGIFNPLLISIIITIIVLAVSGVDYDVYNQGARYLSWFLTPATVCLAIP
LYEQWSLLKKNYKAVVVGIASGVLTSLTTVLVLSKIMNLSHAEYVTLLPKSITTAIGMGVSEELGGYVTITVAVIVVTGV
LGNIFGELICKIFRIKEPIAKGLALGSAAHAIGTAKAMEMGEVEGAMSSLSIAVAGILTVVLSSVFAGFM

Sequences:

>Translated_230_residues
MNDFFRESLFAGVTLSLLSYFIGSVLKKKFKLGIFNPLLISIIITIIVLAVSGVDYDVYNQGARYLSWFLTPATVCLAIP
LYEQWSLLKKNYKAVVVGIASGVLTSLTTVLVLSKIMNLSHAEYVTLLPKSITTAIGMGVSEELGGYVTITVAVIVVTGV
LGNIFGELICKIFRIKEPIAKGLALGSAAHAIGTAKAMEMGEVEGAMSSLSIAVAGILTVVLSSVFAGFM
>Mature_230_residues
MNDFFRESLFAGVTLSLLSYFIGSVLKKKFKLGIFNPLLISIIITIIVLAVSGVDYDVYNQGARYLSWFLTPATVCLAIP
LYEQWSLLKKNYKAVVVGIASGVLTSLTTVLVLSKIMNLSHAEYVTLLPKSITTAIGMGVSEELGGYVTITVAVIVVTGV
LGNIFGELICKIFRIKEPIAKGLALGSAAHAIGTAKAMEMGEVEGAMSSLSIAVAGILTVVLSSVFAGFM

Specific function: Inhibits the expression or activity of extracellular murein hydrolases by interacting, possibly with LrgA, with the holin-like protein CidA. The LrgAB and CidA proteins may affect the proton motive force of the membrane. May be involved in programmed cell

COG id: COG1346

COG function: function code M; Putative effector of murein hydrolase

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CidB/LrgB family. LrgB subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788464, Length=207, Percent_Identity=35.2657004830918, Blast_Score=143, Evalue=7e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007300 [H]

Pfam domain/function: PF04172 LrgB [H]

EC number: NA

Molecular weight: Translated: 24436; Mature: 24436

Theoretical pI: Translated: 8.99; Mature: 8.99

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNDFFRESLFAGVTLSLLSYFIGSVLKKKFKLGIFNPLLISIIITIIVLAVSGVDYDVYN
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCHHHHH
QGARYLSWFLTPATVCLAIPLYEQWSLLKKNYKAVVVGIASGVLTSLTTVLVLSKIMNLS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHEEHHHHHHHHHHHHHHHHHHHHHCCC
HAEYVTLLPKSITTAIGMGVSEELGGYVTITVAVIVVTGVLGNIFGELICKIFRIKEPIA
HHHHHHHHHHHHHHHHCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KGLALGSAAHAIGTAKAMEMGEVEGAMSSLSIAVAGILTVVLSSVFAGFM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MNDFFRESLFAGVTLSLLSYFIGSVLKKKFKLGIFNPLLISIIITIIVLAVSGVDYDVYN
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCHHHHH
QGARYLSWFLTPATVCLAIPLYEQWSLLKKNYKAVVVGIASGVLTSLTTVLVLSKIMNLS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHEEHHHHHHHHHHHHHHHHHHHHHCCC
HAEYVTLLPKSITTAIGMGVSEELGGYVTITVAVIVVTGVLGNIFGELICKIFRIKEPIA
HHHHHHHHHHHHHHHHCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KGLALGSAAHAIGTAKAMEMGEVEGAMSSLSIAVAGILTVVLSSVFAGFM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA