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The map label for this gene is prfB [H]

Identifier: 238916331

GI number: 238916331

Start: 395545

End: 396663

Strand: Direct

Name: prfB [H]

Synonym: EUBELI_00365

Alternate gene names: 238916331

Gene position: 395545-396663 (Clockwise)

Preceding gene: 238916330

Following gene: 238916332

Centisome position: 18.45

GC content: 37.09

Gene sequence:

>1119_bases
ATGGTAGAATTAGACCAGTTCAGATACAGAATTTCAGGATATGATAAGCCTATGGCACAGATTAAGAAATCACTGGATCT
TGACAATAAGCAAAAAAGAATTGAAGAACTGGAAGCAGATATGGAAGCGCCAGGTTTTTGGGATAATCCGGATAAATCAC
AAAATGCTATGAAAGAACTTAAAGGGTTAAAAGATGCATTTGAAAGATATAATGAGCTTGAAACAGGTCTTGATGATGTT
AAGACACTTATTGAGATGGCAGAAGAATCCAATGACCAGTCGCTTATTCCAGAGATAGAAGAAGAGATAACGAATCTCGA
AGATAAGATGGAAAGTTTAAGAATTGAAACATTGCTTTCTGGAGAACATGATGCATGTGATGCCATTCTTACACTTCATG
CAGGAGCAGGTGGAACAGAAAGCTGTGACTGGTGTCAGATGCTTTTCAGAATGTATACAAGATGGGCGGAAAGTCACGGA
TATACAACAGAAACACTTGATTATCTTGAAGGTGAAGAAGCAGGAATTAAGTCGGTTACTATAGAGATAAGAGGAGAGAA
TGCATATGGACATCTGAAATCTGAACATGGTGTTCACAGACTTGTAAGAATATCTCCATTCAATGCGGCAGGTAAGAGAC
AGACTTCTTTCGTATCATGTGACGTAATGCCTGATATTAATCAGGATATAGATATTGAGATTAATGATGATGATTTAAGA
ATTGATACATATCGTTCAAGTGGAGCTGGTGGACAGCATATTAACAAGACATCATCTGCAATCAGAATTACACATCTTCC
AACAGGAATTGTTGTACAGTGTCAGAATGAAAGATCTCAGCACCAGAATAAAGATAAAGCTATGCAGATGTTAAAGGCTA
AGCTCTATCTTTTAAAGGAGGCTGAGAATGCAGAGAATCTATCTGGAATCAGAGGAGATGTCAAGGATATTAATTTTGGA
AGCCAGATAAGAAGTTACGTTCTTCAGCCATATACAATGGTTAAGGATAACAGAACTAATAAGGAAGTTGCTAATGCAGG
AAGTGTTCTGGATGGAAACATTGATCCGTTCATAAATGCATATTTAACATGGATTAGTACGGGAAAAACTGAAGAGTAA

Upstream 100 bases:

>100_bases
TAAGAACTGTTGTGGAAGACAGGCATAAGTTTATAATATGACTTTTGGGACGCGGAATGTCTGGAATATCAGCGTCCGCG
TCTGTTGTTTTGGAGGATAT

Downstream 100 bases:

>100_bases
CAGATATTAAGGTAGTAAACTGAATGATAGTGAAAGGTGGTACATCAGTATGGCGGATTATATTAAACCGGTGATTTTCA
GATTAGGCAATCAACAGTTT

Product: peptide chain release factor RF-2

Products: NA

Alternate protein names: RF-2 [H]

Number of amino acids: Translated: 372; Mature: 372

Protein sequence:

>372_residues
MVELDQFRYRISGYDKPMAQIKKSLDLDNKQKRIEELEADMEAPGFWDNPDKSQNAMKELKGLKDAFERYNELETGLDDV
KTLIEMAEESNDQSLIPEIEEEITNLEDKMESLRIETLLSGEHDACDAILTLHAGAGGTESCDWCQMLFRMYTRWAESHG
YTTETLDYLEGEEAGIKSVTIEIRGENAYGHLKSEHGVHRLVRISPFNAAGKRQTSFVSCDVMPDINQDIDIEINDDDLR
IDTYRSSGAGGQHINKTSSAIRITHLPTGIVVQCQNERSQHQNKDKAMQMLKAKLYLLKEAENAENLSGIRGDVKDINFG
SQIRSYVLQPYTMVKDNRTNKEVANAGSVLDGNIDPFINAYLTWISTGKTEE

Sequences:

>Translated_372_residues
MVELDQFRYRISGYDKPMAQIKKSLDLDNKQKRIEELEADMEAPGFWDNPDKSQNAMKELKGLKDAFERYNELETGLDDV
KTLIEMAEESNDQSLIPEIEEEITNLEDKMESLRIETLLSGEHDACDAILTLHAGAGGTESCDWCQMLFRMYTRWAESHG
YTTETLDYLEGEEAGIKSVTIEIRGENAYGHLKSEHGVHRLVRISPFNAAGKRQTSFVSCDVMPDINQDIDIEINDDDLR
IDTYRSSGAGGQHINKTSSAIRITHLPTGIVVQCQNERSQHQNKDKAMQMLKAKLYLLKEAENAENLSGIRGDVKDINFG
SQIRSYVLQPYTMVKDNRTNKEVANAGSVLDGNIDPFINAYLTWISTGKTEE
>Mature_372_residues
MVELDQFRYRISGYDKPMAQIKKSLDLDNKQKRIEELEADMEAPGFWDNPDKSQNAMKELKGLKDAFERYNELETGLDDV
KTLIEMAEESNDQSLIPEIEEEITNLEDKMESLRIETLLSGEHDACDAILTLHAGAGGTESCDWCQMLFRMYTRWAESHG
YTTETLDYLEGEEAGIKSVTIEIRGENAYGHLKSEHGVHRLVRISPFNAAGKRQTSFVSCDVMPDINQDIDIEINDDDLR
IDTYRSSGAGGQHINKTSSAIRITHLPTGIVVQCQNERSQHQNKDKAMQMLKAKLYLLKEAENAENLSGIRGDVKDINFG
SQIRSYVLQPYTMVKDNRTNKEVANAGSVLDGNIDPFINAYLTWISTGKTEE

Specific function: Peptide chain release factor 2 directs the termination of translation in response to the peptide chain termination codons UGA and UAA [H]

COG id: COG1186

COG function: function code J; Protein chain release factor B

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family [H]

Homologues:

Organism=Homo sapiens, GI166795303, Length=262, Percent_Identity=38.5496183206107, Blast_Score=173, Evalue=2e-43,
Organism=Homo sapiens, GI34577120, Length=370, Percent_Identity=30.2702702702703, Blast_Score=146, Evalue=3e-35,
Organism=Homo sapiens, GI166795305, Length=173, Percent_Identity=40.4624277456647, Blast_Score=126, Evalue=3e-29,
Organism=Escherichia coli, GI2367172, Length=363, Percent_Identity=49.5867768595041, Blast_Score=356, Evalue=1e-99,
Organism=Escherichia coli, GI1787462, Length=246, Percent_Identity=42.6829268292683, Blast_Score=185, Evalue=3e-48,
Organism=Caenorhabditis elegans, GI17542784, Length=297, Percent_Identity=28.2828282828283, Blast_Score=105, Evalue=3e-23,
Organism=Saccharomyces cerevisiae, GI6321295, Length=333, Percent_Identity=32.7327327327327, Blast_Score=143, Evalue=6e-35,
Organism=Drosophila melanogaster, GI19921226, Length=309, Percent_Identity=32.6860841423948, Blast_Score=153, Evalue=2e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR020853
- InterPro:   IPR004374 [H]

Pfam domain/function: PF03462 PCRF; PF00472 RF-1 [H]

EC number: NA

Molecular weight: Translated: 42070; Mature: 42070

Theoretical pI: Translated: 4.52; Mature: 4.52

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVELDQFRYRISGYDKPMAQIKKSLDLDNKQKRIEELEADMEAPGFWDNPDKSQNAMKEL
CCCHHHHHHHHCCCCCHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHH
KGLKDAFERYNELETGLDDVKTLIEMAEESNDQSLIPEIEEEITNLEDKMESLRIETLLS
HHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHC
GEHDACDAILTLHAGAGGTESCDWCQMLFRMYTRWAESHGYTTETLDYLEGEEAGIKSVT
CCCHHHHEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCCEEEE
IEIRGENAYGHLKSEHGVHRLVRISPFNAAGKRQTSFVSCDVMPDINQDIDIEINDDDLR
EEEECCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCEEEEECCCCCCCCEEEEECCCCEE
IDTYRSSGAGGQHINKTSSAIRITHLPTGIVVQCQNERSQHQNKDKAMQMLKAKLYLLKE
EEEECCCCCCCCCCCCCCCEEEEEECCCCEEEEECCHHHHHCCHHHHHHHHHHHHHHHHH
AENAENLSGIRGDVKDINFGSQIRSYVLQPYTMVKDNRTNKEVANAGSVLDGNIDPFINA
CCCCHHHHCCCCCCCCCCCHHHHHHHHHCCHHEECCCCCCHHHHHCCCCCCCCCCHHHHH
YLTWISTGKTEE
HHHHHCCCCCCC
>Mature Secondary Structure
MVELDQFRYRISGYDKPMAQIKKSLDLDNKQKRIEELEADMEAPGFWDNPDKSQNAMKEL
CCCHHHHHHHHCCCCCHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHH
KGLKDAFERYNELETGLDDVKTLIEMAEESNDQSLIPEIEEEITNLEDKMESLRIETLLS
HHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHC
GEHDACDAILTLHAGAGGTESCDWCQMLFRMYTRWAESHGYTTETLDYLEGEEAGIKSVT
CCCHHHHEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCCEEEE
IEIRGENAYGHLKSEHGVHRLVRISPFNAAGKRQTSFVSCDVMPDINQDIDIEINDDDLR
EEEECCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCEEEEECCCCCCCCEEEEECCCCEE
IDTYRSSGAGGQHINKTSSAIRITHLPTGIVVQCQNERSQHQNKDKAMQMLKAKLYLLKE
EEEECCCCCCCCCCCCCCCEEEEEECCCCEEEEECCHHHHHCCHHHHHHHHHHHHHHHHH
AENAENLSGIRGDVKDINFGSQIRSYVLQPYTMVKDNRTNKEVANAGSVLDGNIDPFINA
CCCCHHHHCCCCCCCCCCCHHHHHHHHHCCHHEECCCCCCHHHHHCCCCCCCCCCHHHHH
YLTWISTGKTEE
HHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA