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The map label for this gene is apbE [H]

Identifier: 238916304

GI number: 238916304

Start: 362038

End: 363078

Strand: Direct

Name: apbE [H]

Synonym: EUBELI_00338

Alternate gene names: 238916304

Gene position: 362038-363078 (Clockwise)

Preceding gene: 238916303

Following gene: 238916305

Centisome position: 16.88

GC content: 41.79

Gene sequence:

>1041_bases
ATGAAATATAATATCAGAAAATATATATTGGCAGGAATTACAGCAATTCTTGCAGCGGCTGTTTTGACGGCATGTGGTGG
CAGTAAGCCAGAGAAGGACGATACAGTCCAGAGTACACAGGAAATATTTGCAATGGATACATATATGTCGCTTACTGCAT
ATGGCAGCAATTCAGAGGAAGCAGTAAGTAAAGCAGTTCAGGAGATTAACCGGCTTGATGCAATGTTTTCGGTAGGAAAT
GAGGACAGTGATGTTACTAAGATAAATGAAAATGGAAGCGGTGAAGTATTGGAAGAAACCGCATTTATAATGAACAGGGC
AATGCAGGTATCTAAAGAAACTAAAGGTGCTTTCGATATTACGATATACCAGGTTATGGAGCTGTGGGGATTTACCACCA
AGAATTACAGAGTGCCTGAAAGCAGCGAGATTGCGGATGTACTTAAGCATGTTTCTTATACAAATGTGGAGGTTAACGGG
CAGCAGGTGACATTGTCAGATGGTGCAAGCATTGACCTTGGAGGAATTGCCAAAGGTTATACTTCTTCAAGGGTGATTCA
GATTATGAAGGACTGCGGCATAGAGCACGCAATCATTAATCTTGGAGGCAATGTGCAGGTGCTTGGAACTAAGACTGATG
GCTCAGACTGGAGGATTGCCATACAGAATCCTGACAGTGAAAGCAGTTATCTTGGTGTATTAAGCACTGCTGATAAGGCA
GTTATAACATCAGGTGGATATGAGAGATATTTTGAGCAGGACGGACAGGTATACCATCATATTATAGACCCACAGACAGG
TTATCCATCAGAGAGCGACCTGACCTCGGTTACAATTGTATGCTCTGACGGAACAACAGCAGATGCATTATCAACTGCGC
TTTTTGTAATGGGGCTTGATGGTGCGAAAGAGCTGTATAGAAGTGGCTCACTTGATTTTGAGATGATACTTTATGATGGA
AGCAAAGTATATATGTCAGAGGGAATAGCTGACAGTTTTTCAACCAATATGAACACGGAAATAATTACCAGACAGCCTTG
A

Upstream 100 bases:

>100_bases
CGTGGTGAATGTGTGACCGTATGTGATTGACATTTTAATTATTATGCTATAAAGTTTTATAGAACTAACCAGTATAAGAT
GGAGGATAAAACTTAAAAGC

Downstream 100 bases:

>100_bases
TGAGAATTATTATTAACAGTCTGTATTGACAAATGCAGGGCCTAAAATATATTCTATAGAACAGTTAGTAAAAACTAATC
GAAGATAGATGAAATTACTT

Product: thiamine biosynthesis lipoprotein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 346; Mature: 346

Protein sequence:

>346_residues
MKYNIRKYILAGITAILAAAVLTACGGSKPEKDDTVQSTQEIFAMDTYMSLTAYGSNSEEAVSKAVQEINRLDAMFSVGN
EDSDVTKINENGSGEVLEETAFIMNRAMQVSKETKGAFDITIYQVMELWGFTTKNYRVPESSEIADVLKHVSYTNVEVNG
QQVTLSDGASIDLGGIAKGYTSSRVIQIMKDCGIEHAIINLGGNVQVLGTKTDGSDWRIAIQNPDSESSYLGVLSTADKA
VITSGGYERYFEQDGQVYHHIIDPQTGYPSESDLTSVTIVCSDGTTADALSTALFVMGLDGAKELYRSGSLDFEMILYDG
SKVYMSEGIADSFSTNMNTEIITRQP

Sequences:

>Translated_346_residues
MKYNIRKYILAGITAILAAAVLTACGGSKPEKDDTVQSTQEIFAMDTYMSLTAYGSNSEEAVSKAVQEINRLDAMFSVGN
EDSDVTKINENGSGEVLEETAFIMNRAMQVSKETKGAFDITIYQVMELWGFTTKNYRVPESSEIADVLKHVSYTNVEVNG
QQVTLSDGASIDLGGIAKGYTSSRVIQIMKDCGIEHAIINLGGNVQVLGTKTDGSDWRIAIQNPDSESSYLGVLSTADKA
VITSGGYERYFEQDGQVYHHIIDPQTGYPSESDLTSVTIVCSDGTTADALSTALFVMGLDGAKELYRSGSLDFEMILYDG
SKVYMSEGIADSFSTNMNTEIITRQP
>Mature_346_residues
MKYNIRKYILAGITAILAAAVLTACGGSKPEKDDTVQSTQEIFAMDTYMSLTAYGSNSEEAVSKAVQEINRLDAMFSVGN
EDSDVTKINENGSGEVLEETAFIMNRAMQVSKETKGAFDITIYQVMELWGFTTKNYRVPESSEIADVLKHVSYTNVEVNG
QQVTLSDGASIDLGGIAKGYTSSRVIQIMKDCGIEHAIINLGGNVQVLGTKTDGSDWRIAIQNPDSESSYLGVLSTADKA
VITSGGYERYFEQDGQVYHHIIDPQTGYPSESDLTSVTIVCSDGTTADALSTALFVMGLDGAKELYRSGSLDFEMILYDG
SKVYMSEGIADSFSTNMNTEIITRQP

Specific function: Involved in the conversion of aminoimidazole ribotide (AIR), a purine intermediate, to the 4-amino-5-hydroxymethyl-2- methyl pyrimidine (HMP) moiety of thiamine [H]

COG id: COG1477

COG function: function code H; Membrane-associated lipoprotein involved in thiamine biosynthesis

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ApbE family [H]

Homologues:

Organism=Escherichia coli, GI1788543, Length=346, Percent_Identity=29.7687861271676, Blast_Score=145, Evalue=4e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003374 [H]

Pfam domain/function: PF02424 ApbE [H]

EC number: NA

Molecular weight: Translated: 37538; Mature: 37538

Theoretical pI: Translated: 4.19; Mature: 4.19

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKYNIRKYILAGITAILAAAVLTACGGSKPEKDDTVQSTQEIFAMDTYMSLTAYGSNSEE
CCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHEEEEEECCCHHH
AVSKAVQEINRLDAMFSVGNEDSDVTKINENGSGEVLEETAFIMNRAMQVSKETKGAFDI
HHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEE
TIYQVMELWGFTTKNYRVPESSEIADVLKHVSYTNVEVNGQQVTLSDGASIDLGGIAKGY
HHHHHHHHHCCCCCCEECCCCHHHHHHHHHCCCCEEEECCCEEEECCCCEEECCCCCCCC
TSSRVIQIMKDCGIEHAIINLGGNVQVLGTKTDGSDWRIAIQNPDSESSYLGVLSTADKA
CHHHHHHHHHHCCCCEEEEECCCCEEEEEEECCCCCEEEEEECCCCCCCEEEEEECCCCE
VITSGGYERYFEQDGQVYHHIIDPQTGYPSESDLTSVTIVCSDGTTADALSTALFVMGLD
EEECCCHHHHHHCCCCEEEEEECCCCCCCCCCCCEEEEEEECCCCCHHHHHHHHHEEECC
GAKELYRSGSLDFEMILYDGSKVYMSEGIADSFSTNMNTEIITRQP
CHHHHHHCCCCCEEEEEECCCEEEEECCCCHHHCCCCCCEEEEECC
>Mature Secondary Structure
MKYNIRKYILAGITAILAAAVLTACGGSKPEKDDTVQSTQEIFAMDTYMSLTAYGSNSEE
CCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHEEEEEECCCHHH
AVSKAVQEINRLDAMFSVGNEDSDVTKINENGSGEVLEETAFIMNRAMQVSKETKGAFDI
HHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEE
TIYQVMELWGFTTKNYRVPESSEIADVLKHVSYTNVEVNGQQVTLSDGASIDLGGIAKGY
HHHHHHHHHCCCCCCEECCCCHHHHHHHHHCCCCEEEECCCEEEECCCCEEECCCCCCCC
TSSRVIQIMKDCGIEHAIINLGGNVQVLGTKTDGSDWRIAIQNPDSESSYLGVLSTADKA
CHHHHHHHHHHCCCCEEEEECCCCEEEEEEECCCCCEEEEEECCCCCCCEEEEEECCCCE
VITSGGYERYFEQDGQVYHHIIDPQTGYPSESDLTSVTIVCSDGTTADALSTALFVMGLD
EEECCCHHHHHHCCCCEEEEEECCCCCCCCCCCCEEEEEEECCCCCHHHHHHHHHEEECC
GAKELYRSGSLDFEMILYDGSKVYMSEGIADSFSTNMNTEIITRQP
CHHHHHHCCCCCEEEEEECCCEEEEECCCCHHHCCCCCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9665876 [H]