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The map label for this gene is tuf

Identifier: 238916254

GI number: 238916254

Start: 322473

End: 323666

Strand: Direct

Name: tuf

Synonym: EUBELI_00288

Alternate gene names: 238916254

Gene position: 322473-323666 (Clockwise)

Preceding gene: 238916253

Following gene: 238916255

Centisome position: 15.04

GC content: 43.63

Gene sequence:

>1194_bases
ATGGCAAAGGCTAAATTTGAGAGAACAAAACCACATTGTAACATTGGTACAATCGGACACGTAGATCACGGTAAGACTAC
ATTAACAGCTGCTATCACAAAGACTCTTCATGAGAGACTTGGTACAGGTGAGGCTGTTGCATTCGAGAATATTGATAAGG
CACCAGAGGAAAGAGAAAGAGGTATCACTATCTCTACAGCTCACGTTGAGTATGAGACAGAGAAGAGACACTATGCACAC
GTTGACTGTCCAGGACATGCTGATTATGTAAAGAACATGATCACAGGTGCTGCTCAGATGGATGCAGGTATCCTTGTTGT
TGCTGCTACTGATGGTGTTATGGCTCAGACAAGAGAGCATATCCTTCTTGCTCGTCAGGTTGGTGTTCCTTACATCGTTG
TATTCATGAACAAGTGTGATATGGTTGATGATCCAGAACTTCTTGAATTAGTAGATATGGAAATCAGAGAACTTCTTAAC
GAGTACGGATTCCCAGGAGATGATACTCCAATCATCCAGGGTTCAGCTCTTAAGGCTCTTGAAGATCCTAACAGCGAATG
GGGTGATAAGATCCTTGAGCTTATGCATACTATTGATGAGTATGTTCCAGATCCAGAAAGAGATACAGATAAGCCATTCC
TTATGCCTGTAGAGGACGTATTCTCTATCACAGGACGTGGTACTGTTGCTACTGGTAGAGTAGAGAGAGGTGTTCTCCAC
GTTAATGAGGAAGTTGAAATCGTTGGTATCCACGAAGATATCCGTAAGGTAGTTGTAACTGGTATCGAGATGTTCAGAAA
GCTTCTTGATGAGGCTCAGCCAGGTGATAACATTGGTGCACTTCTTAGAGGTGTTCAGAGAGACGAAATCCAGAGAGGAC
AGGTTCTCTGCAAGCCAGGTTCAATCACACCACACCACAAGTTTACAGCTCAGGTTTACGTATTAACAAAGGACGAGGGT
GGACGTCATACTCCATTCTTCAACAACTACAGACCACAGTTCTACTTCAGAACAACTGATGTAACTGGTGTTTGTGAGCT
TCCAGCTGGTACAGAAATGTGCATGCCTGGTGATAACGTAGAGATGACAGTTGAACTTATCCATAACGTAGCTATGGAGC
AGGGTCTTCGTTTCGCTATCCGTGAGGGTGGTAGAACAGTTGGTTCTGGTGCTGTTGCTACAATCATTGAGTAA

Upstream 100 bases:

>100_bases
AATATTTCACTAAATGAAATATAATAAATATCAGCTATGACAAAGCCCGAGGGGGATACCCCTCAGGGCAATCTAAATAT
TTTTAGGAGGATTTTAAACA

Downstream 100 bases:

>100_bases
TTGTTATTAATACAGTTCTTTCAAGGGTTTCCGAGGTTATCGGAAACCCTTTTTTGCGCGAGCAGCGATGAGAGAACCAG
AGTGTGATAAAAAGAGGAGT

Product: elongation factor Tu

Products: GDP; phosphate

Alternate protein names: EF-Tu

Number of amino acids: Translated: 397; Mature: 396

Protein sequence:

>397_residues
MAKAKFERTKPHCNIGTIGHVDHGKTTLTAAITKTLHERLGTGEAVAFENIDKAPEERERGITISTAHVEYETEKRHYAH
VDCPGHADYVKNMITGAAQMDAGILVVAATDGVMAQTREHILLARQVGVPYIVVFMNKCDMVDDPELLELVDMEIRELLN
EYGFPGDDTPIIQGSALKALEDPNSEWGDKILELMHTIDEYVPDPERDTDKPFLMPVEDVFSITGRGTVATGRVERGVLH
VNEEVEIVGIHEDIRKVVVTGIEMFRKLLDEAQPGDNIGALLRGVQRDEIQRGQVLCKPGSITPHHKFTAQVYVLTKDEG
GRHTPFFNNYRPQFYFRTTDVTGVCELPAGTEMCMPGDNVEMTVELIHNVAMEQGLRFAIREGGRTVGSGAVATIIE

Sequences:

>Translated_397_residues
MAKAKFERTKPHCNIGTIGHVDHGKTTLTAAITKTLHERLGTGEAVAFENIDKAPEERERGITISTAHVEYETEKRHYAH
VDCPGHADYVKNMITGAAQMDAGILVVAATDGVMAQTREHILLARQVGVPYIVVFMNKCDMVDDPELLELVDMEIRELLN
EYGFPGDDTPIIQGSALKALEDPNSEWGDKILELMHTIDEYVPDPERDTDKPFLMPVEDVFSITGRGTVATGRVERGVLH
VNEEVEIVGIHEDIRKVVVTGIEMFRKLLDEAQPGDNIGALLRGVQRDEIQRGQVLCKPGSITPHHKFTAQVYVLTKDEG
GRHTPFFNNYRPQFYFRTTDVTGVCELPAGTEMCMPGDNVEMTVELIHNVAMEQGLRFAIREGGRTVGSGAVATIIE
>Mature_396_residues
AKAKFERTKPHCNIGTIGHVDHGKTTLTAAITKTLHERLGTGEAVAFENIDKAPEERERGITISTAHVEYETEKRHYAHV
DCPGHADYVKNMITGAAQMDAGILVVAATDGVMAQTREHILLARQVGVPYIVVFMNKCDMVDDPELLELVDMEIRELLNE
YGFPGDDTPIIQGSALKALEDPNSEWGDKILELMHTIDEYVPDPERDTDKPFLMPVEDVFSITGRGTVATGRVERGVLHV
NEEVEIVGIHEDIRKVVVTGIEMFRKLLDEAQPGDNIGALLRGVQRDEIQRGQVLCKPGSITPHHKFTAQVYVLTKDEGG
RHTPFFNNYRPQFYFRTTDVTGVCELPAGTEMCMPGDNVEMTVELIHNVAMEQGLRFAIREGGRTVGSGAVATIIE

Specific function: This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis

COG id: COG0050

COG function: function code J; GTPases - translation elongation factors

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. EF-Tu/EF-1A subfamily

Homologues:

Organism=Homo sapiens, GI34147630, Length=396, Percent_Identity=55.8080808080808, Blast_Score=449, Evalue=1e-126,
Organism=Homo sapiens, GI4503475, Length=350, Percent_Identity=34.8571428571429, Blast_Score=166, Evalue=4e-41,
Organism=Homo sapiens, GI4503471, Length=350, Percent_Identity=35.1428571428571, Blast_Score=164, Evalue=1e-40,
Organism=Homo sapiens, GI223555963, Length=447, Percent_Identity=28.6353467561521, Blast_Score=150, Evalue=2e-36,
Organism=Homo sapiens, GI5729864, Length=447, Percent_Identity=28.6353467561521, Blast_Score=150, Evalue=2e-36,
Organism=Homo sapiens, GI194018522, Length=340, Percent_Identity=30.8823529411765, Blast_Score=125, Evalue=5e-29,
Organism=Homo sapiens, GI194097354, Length=340, Percent_Identity=30.8823529411765, Blast_Score=125, Evalue=6e-29,
Organism=Homo sapiens, GI194018520, Length=340, Percent_Identity=30.8823529411765, Blast_Score=125, Evalue=6e-29,
Organism=Homo sapiens, GI54607086, Length=320, Percent_Identity=30.625, Blast_Score=125, Evalue=1e-28,
Organism=Homo sapiens, GI46094014, Length=339, Percent_Identity=29.7935103244838, Blast_Score=124, Evalue=2e-28,
Organism=Homo sapiens, GI4503507, Length=268, Percent_Identity=29.1044776119403, Blast_Score=83, Evalue=5e-16,
Organism=Homo sapiens, GI157426893, Length=250, Percent_Identity=28.4, Blast_Score=75, Evalue=1e-13,
Organism=Homo sapiens, GI53729339, Length=235, Percent_Identity=32.3404255319149, Blast_Score=73, Evalue=5e-13,
Organism=Homo sapiens, GI53729337, Length=235, Percent_Identity=32.3404255319149, Blast_Score=73, Evalue=5e-13,
Organism=Escherichia coli, GI1790412, Length=396, Percent_Identity=72.7272727272727, Blast_Score=574, Evalue=1e-165,
Organism=Escherichia coli, GI1789737, Length=396, Percent_Identity=72.7272727272727, Blast_Score=574, Evalue=1e-165,
Organism=Escherichia coli, GI2367247, Length=338, Percent_Identity=29.8816568047337, Blast_Score=135, Evalue=5e-33,
Organism=Escherichia coli, GI1789108, Length=321, Percent_Identity=31.1526479750779, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI48994988, Length=248, Percent_Identity=32.6612903225806, Blast_Score=93, Evalue=3e-20,
Organism=Caenorhabditis elegans, GI17556456, Length=404, Percent_Identity=51.4851485148515, Blast_Score=385, Evalue=1e-107,
Organism=Caenorhabditis elegans, GI25141371, Length=388, Percent_Identity=41.4948453608247, Blast_Score=321, Evalue=5e-88,
Organism=Caenorhabditis elegans, GI17552884, Length=360, Percent_Identity=34.1666666666667, Blast_Score=159, Evalue=3e-39,
Organism=Caenorhabditis elegans, GI17569207, Length=360, Percent_Identity=34.1666666666667, Blast_Score=159, Evalue=3e-39,
Organism=Caenorhabditis elegans, GI32566303, Length=334, Percent_Identity=33.8323353293413, Blast_Score=136, Evalue=2e-32,
Organism=Caenorhabditis elegans, GI32566629, Length=355, Percent_Identity=29.5774647887324, Blast_Score=127, Evalue=7e-30,
Organism=Caenorhabditis elegans, GI17506081, Length=300, Percent_Identity=28.6666666666667, Blast_Score=125, Evalue=3e-29,
Organism=Caenorhabditis elegans, GI115532067, Length=429, Percent_Identity=27.2727272727273, Blast_Score=121, Evalue=7e-28,
Organism=Caenorhabditis elegans, GI115532065, Length=432, Percent_Identity=27.5462962962963, Blast_Score=120, Evalue=1e-27,
Organism=Caenorhabditis elegans, GI32566301, Length=151, Percent_Identity=39.0728476821192, Blast_Score=94, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI17509919, Length=267, Percent_Identity=26.5917602996255, Blast_Score=79, Evalue=5e-15,
Organism=Caenorhabditis elegans, GI17557151, Length=233, Percent_Identity=29.6137339055794, Blast_Score=74, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6324761, Length=399, Percent_Identity=65.9147869674186, Blast_Score=530, Evalue=1e-151,
Organism=Saccharomyces cerevisiae, GI6325337, Length=354, Percent_Identity=33.8983050847458, Blast_Score=157, Evalue=2e-39,
Organism=Saccharomyces cerevisiae, GI6319594, Length=354, Percent_Identity=33.8983050847458, Blast_Score=157, Evalue=2e-39,
Organism=Saccharomyces cerevisiae, GI6322937, Length=346, Percent_Identity=26.0115606936416, Blast_Score=101, Evalue=2e-22,
Organism=Saccharomyces cerevisiae, GI6320377, Length=353, Percent_Identity=28.328611898017, Blast_Score=98, Evalue=3e-21,
Organism=Saccharomyces cerevisiae, GI6320863, Length=282, Percent_Identity=26.9503546099291, Blast_Score=76, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6323320, Length=240, Percent_Identity=30, Blast_Score=75, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6323098, Length=143, Percent_Identity=35.6643356643357, Blast_Score=70, Evalue=7e-13,
Organism=Drosophila melanogaster, GI281363316, Length=395, Percent_Identity=56.7088607594937, Blast_Score=464, Evalue=1e-131,
Organism=Drosophila melanogaster, GI17864358, Length=395, Percent_Identity=56.7088607594937, Blast_Score=464, Evalue=1e-131,
Organism=Drosophila melanogaster, GI19921738, Length=386, Percent_Identity=47.6683937823834, Blast_Score=390, Evalue=1e-109,
Organism=Drosophila melanogaster, GI45553807, Length=453, Percent_Identity=30.242825607064, Blast_Score=165, Evalue=6e-41,
Organism=Drosophila melanogaster, GI45553816, Length=453, Percent_Identity=30.242825607064, Blast_Score=165, Evalue=6e-41,
Organism=Drosophila melanogaster, GI24651721, Length=453, Percent_Identity=30.242825607064, Blast_Score=165, Evalue=6e-41,
Organism=Drosophila melanogaster, GI17864154, Length=453, Percent_Identity=30.242825607064, Blast_Score=165, Evalue=6e-41,
Organism=Drosophila melanogaster, GI24652838, Length=351, Percent_Identity=35.042735042735, Blast_Score=157, Evalue=8e-39,
Organism=Drosophila melanogaster, GI17137572, Length=351, Percent_Identity=35.042735042735, Blast_Score=157, Evalue=8e-39,
Organism=Drosophila melanogaster, GI19922690, Length=311, Percent_Identity=33.1189710610932, Blast_Score=135, Evalue=4e-32,
Organism=Drosophila melanogaster, GI45550900, Length=339, Percent_Identity=30.9734513274336, Blast_Score=132, Evalue=5e-31,
Organism=Drosophila melanogaster, GI17137380, Length=339, Percent_Identity=30.6784660766962, Blast_Score=125, Evalue=6e-29,
Organism=Drosophila melanogaster, GI24647048, Length=280, Percent_Identity=27.8571428571429, Blast_Score=85, Evalue=7e-17,
Organism=Drosophila melanogaster, GI24647046, Length=280, Percent_Identity=27.8571428571429, Blast_Score=85, Evalue=8e-17,
Organism=Drosophila melanogaster, GI28572034, Length=167, Percent_Identity=37.7245508982036, Blast_Score=70, Evalue=3e-12,
Organism=Drosophila melanogaster, GI78706572, Length=245, Percent_Identity=28.1632653061224, Blast_Score=67, Evalue=2e-11,

Paralogues:

None

Copy number: 20568 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): EFTU_EUBE2 (C4Z2R9)

Other databases:

- EMBL:   CP001104
- RefSeq:   YP_002929771.1
- GeneID:   7957181
- GenomeReviews:   CP001104_GR
- KEGG:   eel:EUBELI_00288
- OMA:   FEGQVYV
- ProtClustDB:   PRK00049
- GO:   GO:0005737
- HAMAP:   MF_00118_B
- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR009001
- InterPro:   IPR004161
- InterPro:   IPR004541
- InterPro:   IPR004160
- InterPro:   IPR009000
- PANTHER:   PTHR23115:SF31
- PRINTS:   PR00315
- TIGRFAMs:   TIGR00485
- TIGRFAMs:   TIGR00231

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF03143 GTP_EFTU_D3; SSF50465 Elong_init_C; SSF50447 Translat_factor

EC number: 3.6.5.3

Molecular weight: Translated: 43998; Mature: 43866

Theoretical pI: Translated: 4.85; Mature: 4.85

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKAKFERTKPHCNIGTIGHVDHGKTTLTAAITKTLHERLGTGEAVAFENIDKAPEERER
CCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEHHCCCCCHHHHHC
GITISTAHVEYETEKRHYAHVDCPGHADYVKNMITGAAQMDAGILVVAATDGVMAQTREH
CCEEEEEEEEEECCCCEEEEECCCCHHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHH
ILLARQVGVPYIVVFMNKCDMVDDPELLELVDMEIRELLNEYGFPGDDTPIIQGSALKAL
HHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCEECCCHHHHH
EDPNSEWGDKILELMHTIDEYVPDPERDTDKPFLMPVEDVFSITGRGTVATGRVERGVLH
CCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEHHHHHHHCCCCEEEECCHHCCEEE
VNEEVEIVGIHEDIRKVVVTGIEMFRKLLDEAQPGDNIGALLRGVQRDEIQRGQVLCKPG
ECCCEEEEECHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCHHHHCCCCEEECCC
SITPHHKFTAQVYVLTKDEGGRHTPFFNNYRPQFYFRTTDVTGVCELPAGTEMCMPGDNV
CCCCCCEEEEEEEEEEECCCCCCCCCCCCCCCEEEEEECCCCEEEECCCCCCCCCCCCCH
EMTVELIHNVAMEQGLRFAIREGGRTVGSGAVATIIE
HHHHHHHHHHHHHHCCHHHHHCCCCCCCCCCEEEEEC
>Mature Secondary Structure 
AKAKFERTKPHCNIGTIGHVDHGKTTLTAAITKTLHERLGTGEAVAFENIDKAPEERER
CCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEHHCCCCCHHHHHC
GITISTAHVEYETEKRHYAHVDCPGHADYVKNMITGAAQMDAGILVVAATDGVMAQTREH
CCEEEEEEEEEECCCCEEEEECCCCHHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHH
ILLARQVGVPYIVVFMNKCDMVDDPELLELVDMEIRELLNEYGFPGDDTPIIQGSALKAL
HHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCEECCCHHHHH
EDPNSEWGDKILELMHTIDEYVPDPERDTDKPFLMPVEDVFSITGRGTVATGRVERGVLH
CCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEHHHHHHHCCCCEEEECCHHCCEEE
VNEEVEIVGIHEDIRKVVVTGIEMFRKLLDEAQPGDNIGALLRGVQRDEIQRGQVLCKPG
ECCCEEEEECHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCHHHHCCCCEEECCC
SITPHHKFTAQVYVLTKDEGGRHTPFFNNYRPQFYFRTTDVTGVCELPAGTEMCMPGDNV
CCCCCCEEEEEEEEEEECCCCCCCCCCCCCCCEEEEEECCCCEEEECCCCCCCCCCCCCH
EMTVELIHNVAMEQGLRFAIREGGRTVGSGAVATIIE
HHHHHHHHHHHHHHCCHHHHHCCCCCCCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: GTP; H2O

Specific reaction: GTP + H2O = GDP + phosphate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA