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The map label for this gene is vanHB [H]

Identifier: 238916147

GI number: 238916147

Start: 211105

End: 212091

Strand: Direct

Name: vanHB [H]

Synonym: EUBELI_00181

Alternate gene names: 238916147

Gene position: 211105-212091 (Clockwise)

Preceding gene: 238916146

Following gene: 238916149

Centisome position: 9.85

GC content: 40.63

Gene sequence:

>987_bases
ATGAAGATAGCAGCATTCAGCGTTCGTCAAGATGAAAAACAGTATTTTGATACATTTGCAAATGTATACAAGGTGGAATT
ACAGATTAATAGGAGCGGATTTACAGCAGATGATATAGAAAGTGTCAAAGGCTGTGAGGCTATGTCGGTGTGCGATGGCA
TGTGTGACTTGTCAGCACCTGTTCTTGAAAAGCTTGCAGCAGAGGGTGTAAAGTACATCGGTTTCAGGACAATCGGATAT
AACAGTGTTGACCTTGAGGCAGCAAAGCGCCTTGGAATCCGTGTTGCACACAGCGGATATTCGCCATATTCAGTTGCCAA
TTATACAGTAATGCTTATGCTTATGTGTATCCGCAAGGCTTTGTATGTAATGATGCGTTCACACACAGCAGATTATTCAC
TTGGTCCTATATGTGGACGTGAAATGCAGAATATGACAGTAGGTATTATCGGAACAGGAAGAATTGGAAAGGCAGTAATT
AAGAATCTTTCAGGATTTGGATGTAAGATTATTGCGTATGACCCATATCCGGCAAAAGACCTTGAAAATGTTGAGTATGT
AACTCTTGATGAATTGTATGCCAGAGCAGATATTATCTCGCTTCATACTTTCTTAAGTGATGAGACATATCATATGATTA
ATAAGGATTCAATTGCAAAAATGAAGGATGGCGTTATCCTTATAAATGCGGCAAGAGGCGCGCTTGTTGATACAAAGGAT
CTGATTGAGGCTGTTGAGAGTGGCAAGATTGGTTCAGTTGGAACTGACTGCTGCGAGGGTGAGGATGAATTTATCCGTAC
AGACCGTAAGTATGATGACCTTGTTGTTAATCACGATTACATCATACTTAAGTCTTTTCAGAATACTATTGTCACACCTC
ATGTTGCATTTTTCACAGATCAGGCTGTGTCTGACATGGTTGAGTCTTCAGTTAAGAGTGTTGTGCAGTTTGCAGCCGGT
GAAGACACACCATTAGAGGTGCATTAA

Upstream 100 bases:

>100_bases
ACTTAATTATTCGACACCTTCTGATGAGCAGATTGTTAAGGGAATCGAAATTCTTGGAAAACTTACATATGAGTGGATTA
AGTAGGAGGAGTGAAAAGTT

Downstream 100 bases:

>100_bases
TTATTCAAATTTTTGAAATATTCTTCAAGCTGGTGCTCGAAGATTCGCTGATTTTCTGAGCAGAATGAGCCAAGCGGCTG
ATAATAAATGCTTCTGAAAT

Product: D-lactate dehydrogenase

Products: pyruvate; NADH; H+

Alternate protein names: Vancomycin B-type resistance protein vanHB [H]

Number of amino acids: Translated: 328; Mature: 328

Protein sequence:

>328_residues
MKIAAFSVRQDEKQYFDTFANVYKVELQINRSGFTADDIESVKGCEAMSVCDGMCDLSAPVLEKLAAEGVKYIGFRTIGY
NSVDLEAAKRLGIRVAHSGYSPYSVANYTVMLMLMCIRKALYVMMRSHTADYSLGPICGREMQNMTVGIIGTGRIGKAVI
KNLSGFGCKIIAYDPYPAKDLENVEYVTLDELYARADIISLHTFLSDETYHMINKDSIAKMKDGVILINAARGALVDTKD
LIEAVESGKIGSVGTDCCEGEDEFIRTDRKYDDLVVNHDYIILKSFQNTIVTPHVAFFTDQAVSDMVESSVKSVVQFAAG
EDTPLEVH

Sequences:

>Translated_328_residues
MKIAAFSVRQDEKQYFDTFANVYKVELQINRSGFTADDIESVKGCEAMSVCDGMCDLSAPVLEKLAAEGVKYIGFRTIGY
NSVDLEAAKRLGIRVAHSGYSPYSVANYTVMLMLMCIRKALYVMMRSHTADYSLGPICGREMQNMTVGIIGTGRIGKAVI
KNLSGFGCKIIAYDPYPAKDLENVEYVTLDELYARADIISLHTFLSDETYHMINKDSIAKMKDGVILINAARGALVDTKD
LIEAVESGKIGSVGTDCCEGEDEFIRTDRKYDDLVVNHDYIILKSFQNTIVTPHVAFFTDQAVSDMVESSVKSVVQFAAG
EDTPLEVH
>Mature_328_residues
MKIAAFSVRQDEKQYFDTFANVYKVELQINRSGFTADDIESVKGCEAMSVCDGMCDLSAPVLEKLAAEGVKYIGFRTIGY
NSVDLEAAKRLGIRVAHSGYSPYSVANYTVMLMLMCIRKALYVMMRSHTADYSLGPICGREMQNMTVGIIGTGRIGKAVI
KNLSGFGCKIIAYDPYPAKDLENVEYVTLDELYARADIISLHTFLSDETYHMINKDSIAKMKDGVILINAARGALVDTKD
LIEAVESGKIGSVGTDCCEGEDEFIRTDRKYDDLVVNHDYIILKSFQNTIVTPHVAFFTDQAVSDMVESSVKSVVQFAAG
EDTPLEVH

Specific function: Required for high-level resistance to glycopeptides antibiotics. Catalyzes the reduction of 2-keto acids to 2-D- hydroxy acids that give rise to peptidoglycan precursors that terminate in the depsipeptide D-alanine-2-lactate rather than the dipeptide D-al

COG id: COG1052

COG function: function code CHR; Lactate dehydrogenase and related dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI145580578, Length=270, Percent_Identity=32.962962962963, Blast_Score=117, Evalue=1e-26,
Organism=Homo sapiens, GI4557499, Length=270, Percent_Identity=32.962962962963, Blast_Score=117, Evalue=1e-26,
Organism=Homo sapiens, GI145580575, Length=319, Percent_Identity=30.4075235109718, Blast_Score=117, Evalue=2e-26,
Organism=Homo sapiens, GI6912396, Length=273, Percent_Identity=30.7692307692308, Blast_Score=115, Evalue=6e-26,
Organism=Homo sapiens, GI23308577, Length=243, Percent_Identity=30.0411522633745, Blast_Score=113, Evalue=2e-25,
Organism=Homo sapiens, GI61743967, Length=270, Percent_Identity=30, Blast_Score=113, Evalue=3e-25,
Organism=Homo sapiens, GI4557497, Length=270, Percent_Identity=30, Blast_Score=113, Evalue=3e-25,
Organism=Escherichia coli, GI1787645, Length=333, Percent_Identity=37.2372372372372, Blast_Score=228, Evalue=4e-61,
Organism=Escherichia coli, GI87082289, Length=274, Percent_Identity=28.1021897810219, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI1789279, Length=245, Percent_Identity=29.3877551020408, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1788660, Length=205, Percent_Identity=32.1951219512195, Blast_Score=76, Evalue=4e-15,
Organism=Caenorhabditis elegans, GI17532191, Length=264, Percent_Identity=28.7878787878788, Blast_Score=110, Evalue=8e-25,
Organism=Caenorhabditis elegans, GI25147481, Length=277, Percent_Identity=30.3249097472924, Blast_Score=102, Evalue=2e-22,
Organism=Saccharomyces cerevisiae, GI6324055, Length=246, Percent_Identity=33.3333333333333, Blast_Score=118, Evalue=1e-27,
Organism=Saccharomyces cerevisiae, GI6322116, Length=287, Percent_Identity=31.3588850174216, Blast_Score=102, Evalue=7e-23,
Organism=Saccharomyces cerevisiae, GI6320925, Length=287, Percent_Identity=31.3588850174216, Blast_Score=102, Evalue=7e-23,
Organism=Saccharomyces cerevisiae, GI6324964, Length=205, Percent_Identity=27.3170731707317, Blast_Score=82, Evalue=1e-16,
Organism=Saccharomyces cerevisiae, GI6324980, Length=139, Percent_Identity=28.0575539568345, Blast_Score=68, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6325144, Length=190, Percent_Identity=31.0526315789474, Blast_Score=66, Evalue=9e-12,
Organism=Drosophila melanogaster, GI24646446, Length=316, Percent_Identity=29.1139240506329, Blast_Score=120, Evalue=1e-27,
Organism=Drosophila melanogaster, GI24646448, Length=316, Percent_Identity=29.1139240506329, Blast_Score=120, Evalue=1e-27,
Organism=Drosophila melanogaster, GI24646452, Length=316, Percent_Identity=29.1139240506329, Blast_Score=120, Evalue=1e-27,
Organism=Drosophila melanogaster, GI24646450, Length=316, Percent_Identity=29.1139240506329, Blast_Score=120, Evalue=1e-27,
Organism=Drosophila melanogaster, GI62472511, Length=316, Percent_Identity=28.7974683544304, Blast_Score=119, Evalue=2e-27,
Organism=Drosophila melanogaster, GI28574286, Length=295, Percent_Identity=27.7966101694915, Blast_Score=102, Evalue=4e-22,
Organism=Drosophila melanogaster, GI24585514, Length=304, Percent_Identity=27.6315789473684, Blast_Score=99, Evalue=5e-21,
Organism=Drosophila melanogaster, GI28574282, Length=304, Percent_Identity=27.6315789473684, Blast_Score=99, Evalue=5e-21,
Organism=Drosophila melanogaster, GI28574284, Length=304, Percent_Identity=27.6315789473684, Blast_Score=99, Evalue=5e-21,
Organism=Drosophila melanogaster, GI45552429, Length=302, Percent_Identity=27.8145695364238, Blast_Score=99, Evalue=6e-21,
Organism=Drosophila melanogaster, GI45551003, Length=302, Percent_Identity=27.8145695364238, Blast_Score=98, Evalue=6e-21,
Organism=Drosophila melanogaster, GI19921140, Length=254, Percent_Identity=26.3779527559055, Blast_Score=95, Evalue=8e-20,
Organism=Drosophila melanogaster, GI28571528, Length=274, Percent_Identity=26.6423357664234, Blast_Score=91, Evalue=1e-18,
Organism=Drosophila melanogaster, GI24585516, Length=212, Percent_Identity=25.4716981132075, Blast_Score=69, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006139
- InterPro:   IPR006140
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00389 2-Hacid_dh; PF02826 2-Hacid_dh_C [H]

EC number: 1.1.1.28

Molecular weight: Translated: 36229; Mature: 36229

Theoretical pI: Translated: 4.81; Mature: 4.81

Prosite motif: PS00065 D_2_HYDROXYACID_DH_1 ; PS00671 D_2_HYDROXYACID_DH_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
6.4 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
6.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIAAFSVRQDEKQYFDTFANVYKVELQINRSGFTADDIESVKGCEAMSVCDGMCDLSAP
CCEEEECCCHHHHHHHHHHHHEEEEEEEECCCCCCHHHHHHHCCCHHHHHHCCHHCCCHH
VLEKLAAEGVKYIGFRTIGYNSVDLEAAKRLGIRVAHSGYSPYSVANYTVMLMLMCIRKA
HHHHHHHCCCEEEEEEECCCCCCCHHHHHHCCEEEEECCCCCCHHHHHHHHHHHHHHHHH
LYVMMRSHTADYSLGPICGREMQNMTVGIIGTGRIGKAVIKNLSGFGCKIIAYDPYPAKD
HHHHHHHCCCCCCCCCCCCHHHCCCEEEEEECCCHHHHHHHCCCCCCEEEEEECCCCCCC
LENVEYVTLDELYARADIISLHTFLSDETYHMINKDSIAKMKDGVILINAARGALVDTKD
CCCCCEEEHHHHHHHHHHHHHHHHHCCCHHEECCCCHHHHHCCCEEEEEECCCCCCCHHH
LIEAVESGKIGSVGTDCCEGEDEFIRTDRKYDDLVVNHDYIILKSFQNTIVTPHVAFFTD
HHHHHHCCCCCCCCCHHCCCCHHHHHCCCCCCCEEECCCEEEEECCCCCCCCCHHHHHHH
QAVSDMVESSVKSVVQFAAGEDTPLEVH
HHHHHHHHHHHHHHHHHHCCCCCCEECC
>Mature Secondary Structure
MKIAAFSVRQDEKQYFDTFANVYKVELQINRSGFTADDIESVKGCEAMSVCDGMCDLSAP
CCEEEECCCHHHHHHHHHHHHEEEEEEEECCCCCCHHHHHHHCCCHHHHHHCCHHCCCHH
VLEKLAAEGVKYIGFRTIGYNSVDLEAAKRLGIRVAHSGYSPYSVANYTVMLMLMCIRKA
HHHHHHHCCCEEEEEEECCCCCCCHHHHHHCCEEEEECCCCCCHHHHHHHHHHHHHHHHH
LYVMMRSHTADYSLGPICGREMQNMTVGIIGTGRIGKAVIKNLSGFGCKIIAYDPYPAKD
HHHHHHHCCCCCCCCCCCCHHHCCCEEEEEECCCHHHHHHHCCCCCCEEEEEECCCCCCC
LENVEYVTLDELYARADIISLHTFLSDETYHMINKDSIAKMKDGVILINAARGALVDTKD
CCCCCEEEHHHHHHHHHHHHHHHHHCCCHHEECCCCHHHHHCCCEEEEEECCCCCCCHHH
LIEAVESGKIGSVGTDCCEGEDEFIRTDRKYDDLVVNHDYIILKSFQNTIVTPHVAFFTD
HHHHHHCCCCCCCCCHHCCCCHHHHHCCCCCCCEEECCCEEEEECCCCCCCCCHHHHHHH
QAVSDMVESSVKSVVQFAAGEDTPLEVH
HHHHHHHHHHHHHHHHHHCCCCCCEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: (R)-lactate; NAD+

Specific reaction: (R)-lactate + NAD+ = pyruvate + NADH + H+

General reaction: Redox reaction [C]

Inhibitor: Pyruvate [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8631706; 12663927 [H]