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The map label for this gene is opuE [H]

Identifier: 238916141

GI number: 238916141

Start: 203568

End: 205115

Strand: Reverse

Name: opuE [H]

Synonym: EUBELI_00175

Alternate gene names: 238916141

Gene position: 205115-203568 (Counterclockwise)

Preceding gene: 238916142

Following gene: 238916140

Centisome position: 9.57

GC content: 41.15

Gene sequence:

>1548_bases
ATGGGGGTTTTTATGTTAAAACTGGTTATTCTTATTATCTACTTTGCGGTGCTGATAGGCATTGGATTATACTGCCGCAA
GCATGCAACTGATGTTAATGGTTTCGTTCTCGGTGGAAGAAGCGTTGGTCCGTGGCTCACCGCATTTGCATATGGAACAT
CTTATTTCTCGGCTGTTGTATTCGTTGGATACGCAGGCCAGTTTGGCTGGAAATACGGCATTGCCGCAACCTGGGCTGGT
ATCGGCAACGCAATTATAGGTTCACTTCTTGCATGGGTTGTACTTGGCAGAAGAACAAGAATTATGAGTCAGCACCTTGA
TTCAGCAACCATGCCACAGTATTTTGGAAAAAGATTCGGAAGCAATCCATTAAAAATCGGTGCTTCTGTTATAATATTCA
TTTTTCTGATTCCATACACAGCTTCACTTTACAATGGACTTTCAAGACTTTTCGGTATGGCTTTCAACATTGATTACAGC
GTCTGTATCATAATCATGGCAACTCTTACCGCAATCTATGTAATTGCCGGCGGTTACATGGCTACTGCAATCAATGATTT
CATTCAGGGAATTATCATGTTATTCGGTATCGTTGCAGTTATTGCTGCCGTAATCCACTCTAAGGGCGGCTTCATGTCTG
CACTGCAGGGTCTTGCAAATGTGTCTGACCCGGCAGTAAGCGATACTCCCGGTATATTTGCTTCATTTTTCGGACCTGAC
CCATTCAACCTTTTATGCGTTGTAATCCTTACATCACTTGGAACATGGGGACTTCCTCAGATGGTTCAGAAATTCTATGC
CATTGATAATGAAGCTTCTATACAGAAGGGTACTGTAATCTCAACAATATTTGCGCTTATAGTTTCAGGCGGATGCTATT
TTCTCGGAGGTTTCGGAAGATTATTCTCTGACCAGATAGATGTCAAGGCTAACGGCTTCGACTCAATAATACCAACAATG
CTTTCTAATTTAAGTCCTGCGCTTATTGCATTAGTTGTAATTCTCGTGTTGTCAGCTTCAATGTCTACACTTTCATCACT
TGTAATAGCTTCTAGCTCAACACTTACTATTGACTTCTTAAAAGATAACTTTATCAAGAATATGAGTGAAAAGAAACAGG
TATTATACATAAGAATTCTTGTTGTTGTATTCATTGCGATATCTGCAATACTTGCACTTATCCAGTACAAGAGTTCTGTA
ACATTTATTGCACAGCTTATGGGAATATCTTGGGGTGCACTTGCAGGTTCATTCCTTGCACCATTTATGTTCTCGCTTTA
CTCTAAGAAGGTTTCCAAAGCATCATGCTGGGCATGCTTTTTATTCAGCTCAGTACTTATGCTTGCTAACATCTTCTTCA
GAGCAGGCTTCCCAACATGGCTCCAGTCACCTATCAACTGCGGTGCATTTGCAATGTTTGCCGGAATGATTATCGTTCCT
GTAGTCAGCCTGTTTACACCGAAGCCAGATAAAGAACTTGTCGACAGCGCATTTGCATGCTACGAAAAAGAAACAGAAGT
GCCACAGAAGACAGCACTTGGAAAATAA

Upstream 100 bases:

>100_bases
TGCCAATCCCTCCCCAATATGCTACAATCCATTTATGTTTACTTTATTGATTTAAAGCGTTACGCTTTAAACCCGTGAAG
TAAGCTATAATACATTTAAT

Downstream 100 bases:

>100_bases
TTCGCAACATATCTAATATTTATATACCCTTTACAACAAAACCTCCTTGTGCTATAACAACCAAAGTTATAAAACACAAG
GAGGTTATTTATGGCATCAA

Product: solute:Na+ symporter

Products: NA

Alternate protein names: Sodium/proline symporter [H]

Number of amino acids: Translated: 515; Mature: 514

Protein sequence:

>515_residues
MGVFMLKLVILIIYFAVLIGIGLYCRKHATDVNGFVLGGRSVGPWLTAFAYGTSYFSAVVFVGYAGQFGWKYGIAATWAG
IGNAIIGSLLAWVVLGRRTRIMSQHLDSATMPQYFGKRFGSNPLKIGASVIIFIFLIPYTASLYNGLSRLFGMAFNIDYS
VCIIIMATLTAIYVIAGGYMATAINDFIQGIIMLFGIVAVIAAVIHSKGGFMSALQGLANVSDPAVSDTPGIFASFFGPD
PFNLLCVVILTSLGTWGLPQMVQKFYAIDNEASIQKGTVISTIFALIVSGGCYFLGGFGRLFSDQIDVKANGFDSIIPTM
LSNLSPALIALVVILVLSASMSTLSSLVIASSSTLTIDFLKDNFIKNMSEKKQVLYIRILVVVFIAISAILALIQYKSSV
TFIAQLMGISWGALAGSFLAPFMFSLYSKKVSKASCWACFLFSSVLMLANIFFRAGFPTWLQSPINCGAFAMFAGMIIVP
VVSLFTPKPDKELVDSAFACYEKETEVPQKTALGK

Sequences:

>Translated_515_residues
MGVFMLKLVILIIYFAVLIGIGLYCRKHATDVNGFVLGGRSVGPWLTAFAYGTSYFSAVVFVGYAGQFGWKYGIAATWAG
IGNAIIGSLLAWVVLGRRTRIMSQHLDSATMPQYFGKRFGSNPLKIGASVIIFIFLIPYTASLYNGLSRLFGMAFNIDYS
VCIIIMATLTAIYVIAGGYMATAINDFIQGIIMLFGIVAVIAAVIHSKGGFMSALQGLANVSDPAVSDTPGIFASFFGPD
PFNLLCVVILTSLGTWGLPQMVQKFYAIDNEASIQKGTVISTIFALIVSGGCYFLGGFGRLFSDQIDVKANGFDSIIPTM
LSNLSPALIALVVILVLSASMSTLSSLVIASSSTLTIDFLKDNFIKNMSEKKQVLYIRILVVVFIAISAILALIQYKSSV
TFIAQLMGISWGALAGSFLAPFMFSLYSKKVSKASCWACFLFSSVLMLANIFFRAGFPTWLQSPINCGAFAMFAGMIIVP
VVSLFTPKPDKELVDSAFACYEKETEVPQKTALGK
>Mature_514_residues
GVFMLKLVILIIYFAVLIGIGLYCRKHATDVNGFVLGGRSVGPWLTAFAYGTSYFSAVVFVGYAGQFGWKYGIAATWAGI
GNAIIGSLLAWVVLGRRTRIMSQHLDSATMPQYFGKRFGSNPLKIGASVIIFIFLIPYTASLYNGLSRLFGMAFNIDYSV
CIIIMATLTAIYVIAGGYMATAINDFIQGIIMLFGIVAVIAAVIHSKGGFMSALQGLANVSDPAVSDTPGIFASFFGPDP
FNLLCVVILTSLGTWGLPQMVQKFYAIDNEASIQKGTVISTIFALIVSGGCYFLGGFGRLFSDQIDVKANGFDSIIPTML
SNLSPALIALVVILVLSASMSTLSSLVIASSSTLTIDFLKDNFIKNMSEKKQVLYIRILVVVFIAISAILALIQYKSSVT
FIAQLMGISWGALAGSFLAPFMFSLYSKKVSKASCWACFLFSSVLMLANIFFRAGFPTWLQSPINCGAFAMFAGMIIVPV
VSLFTPKPDKELVDSAFACYEKETEVPQKTALGK

Specific function: Catalyzes the sodium-dependent uptake of extracellular proline [H]

COG id: COG0591

COG function: function code ER; Na+/proline symporter

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sodium:solute symporter (SSF) (TC 2.A.21) family [H]

Homologues:

Organism=Homo sapiens, GI310128183, Length=516, Percent_Identity=28.8759689922481, Blast_Score=147, Evalue=2e-35,
Organism=Homo sapiens, GI14140236, Length=470, Percent_Identity=25.1063829787234, Blast_Score=94, Evalue=4e-19,
Organism=Homo sapiens, GI4507031, Length=555, Percent_Identity=24.5045045045045, Blast_Score=78, Evalue=2e-14,
Organism=Homo sapiens, GI206597483, Length=361, Percent_Identity=25.207756232687, Blast_Score=74, Evalue=3e-13,
Organism=Homo sapiens, GI206597487, Length=361, Percent_Identity=25.207756232687, Blast_Score=74, Evalue=3e-13,
Organism=Homo sapiens, GI4507033, Length=481, Percent_Identity=22.2453222453222, Blast_Score=70, Evalue=5e-12,
Organism=Homo sapiens, GI17941285, Length=464, Percent_Identity=24.5689655172414, Blast_Score=70, Evalue=7e-12,
Organism=Homo sapiens, GI110835708, Length=556, Percent_Identity=22.841726618705, Blast_Score=68, Evalue=2e-11,
Organism=Escherichia coli, GI1787251, Length=380, Percent_Identity=27.8947368421053, Blast_Score=129, Evalue=3e-31,
Organism=Escherichia coli, GI87082237, Length=449, Percent_Identity=26.5033407572383, Blast_Score=125, Evalue=9e-30,
Organism=Escherichia coli, GI1790503, Length=470, Percent_Identity=19.5744680851064, Blast_Score=69, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011851
- InterPro:   IPR001734
- InterPro:   IPR018212
- InterPro:   IPR019900 [H]

Pfam domain/function: PF00474 SSF [H]

EC number: NA

Molecular weight: Translated: 55486; Mature: 55355

Theoretical pI: Translated: 9.31; Mature: 9.31

Prosite motif: PS50283 NA_SOLUT_SYMP_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHCCCCCHHHHHHHHHHHHHHCCCCCHHCCCC
>Mature Secondary Structure 
GVFMLKLVILIIYFAVLIGIGLYCRKHATDVNGFVLGGRSVGPWLTAFAYGTSYFSAVV
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHH
FVGYAGQFGWKYGIAATWAGIGNAIIGSLLAWVVLGRRTRIMSQHLDSATMPQYFGKRFG
HHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHC
SNPLKIGASVIIFIFLIPYTASLYNGLSRLFGMAFNIDYSVCIIIMATLTAIYVIAGGYM
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCHH
ATAINDFIQGIIMLFGIVAVIAAVIHSKGGFMSALQGLANVSDPAVSDTPGIFASFFGPD
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCCCHHHHHCCCC
PFNLLCVVILTSLGTWGLPQMVQKFYAIDNEASIQKGTVISTIFALIVSGGCYFLGGFGR
HHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCHHHHHCHHH
LFSDQIDVKANGFDSIIPTMLSNLSPALIALVVILVLSASMSTLSSLVIASSSTLTIDFL
HHCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEHHH
KDNFIKNMSEKKQVLYIRILVVVFIAISAILALIQYKSSVTFIAQLMGISWGALAGSFLA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
PFMFSLYSKKVSKASCWACFLFSSVLMLANIFFRAGFPTWLQSPINCGAFAMFAGMIIVP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCCCCCHHHHHHHHHHHH
VVSLFTPKPDKELVDSAFACYEKETEVPQKTALGK
HHHHHCCCCCHHHHHHHHHHHHHHCCCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8969499; 9384377 [H]