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The map label for this gene is metK [H]

Identifier: 238916137

GI number: 238916137

Start: 199729

End: 200973

Strand: Reverse

Name: metK [H]

Synonym: EUBELI_00171

Alternate gene names: 238916137

Gene position: 200973-199729 (Counterclockwise)

Preceding gene: 238916139

Following gene: 238916133

Centisome position: 9.37

GC content: 41.12

Gene sequence:

>1245_bases
ATGAGAATAGACACGATAAGGTATGGGATAGACATATTCACGATAAAACAAAAAGGAGATAACATGGAAAAAAGATTATT
TACTTCAGAATCAGTAACAGAAGGACATCCAGATAAGATCTGCGACCAGATTTCAGATGCAGTATTAGATGCTCTCTATG
AGCAGGATCCATACTCAAGAGTAGCCTGCGAAACTCTTACTAACACAGGTTTTATTATGGTAATGGGCGAGGTTACAACT
AAGGCTAACATAGACATTCCTTCTATTGTAAGAAAAACAGTTACAGATATTGGTTATGACAGCTCTGATAAGGGATTTGA
TGGTAATACCTGTGCTGTTATGGTTGCATTAGACAAGCAGTCAGCTGATATTGCTATGGGTGTTGATAAGGCTCTTGAAG
CCAAAGACGGCGAAATTGATGATTCAGAGGGTGGCGCCGGCGACCAGGGTATGATGTTTGGTTACGCAACTAACGAAACT
CCTGAATTCATGCCTTACTCTTCTGCACTTGCACAGAAGCTTTCTAAGCAGCTTACAAAGGTTCGCAAGGATGGAACTCT
TTCTTACTTAAGACCAGACGGAAAGACTCAGGTAACAGTTGAATACGATGAAAACAAAAAGCCAATAAGACTTGATGCTA
TCGTTGTTTCATCACAGCATGCTCCAGAAGTATCTCAGGAACAGATTCATGAGGATATTAAGAAATATGTTATTGACGCT
ATCGTTGACCCAGCTATGATTGACGCTGATACTAAGATATACATCAATCCTACAGGCCGTTTTGTAATTGGCGGTCCTAA
CGGAGATTCAGGTCTTACTGGTCGTAAGATTATCGTTGATACTTACGGTGGTGCCGCAAGACATGGTGGTGGTGCTTTCT
CTGGTAAAGACTGCACTAAGGTTGACCGTTCTGCTGCATACGCTGCAAGATATGTTGCAAAGAATATTGTTGCTGCCGGA
CTTGCTGACAGATGTGAAATCCAGCTTTCATATGCAATCGGTGTCGCAAGACCAACATCTATCATGGTTGATACATTTGG
TACAGGAAAGCTTGATGAAGAAAAAATCGTTGAAATCATCCGTGAGAACTTCGATTTAAGACCTAACGGAATCATTAAGA
TGCTCGACTTAAGAAGACCTATCTATAAGCAGACAGCTGCTTATGGTCACTTTGGACGCACAGATCTTAATCTCCCTTGG
GAGAAGCTTGACAAGGTTGATGTACTTGCTAAGTATCTTGGTTAA

Upstream 100 bases:

>100_bases
ATTTCGATACAGTCTATCGGGTCCCTTGTCGGGGACCTTTTTTTGTGCGAGTAGCGATAGCAATGTAATTCTGGCGTCTG
AGCTACGAGCGCGAGTAGCG

Downstream 100 bases:

>100_bases
TGATATTTAAATAGAATAACAGACACATATAGTTTTCATTGATGGGCACCCCACATACATCTATATGTGTCTGTTATTTT
AATTTGTCTTAATATTTTAA

Product: S-adenosylmethionine synthetase

Products: NA

Alternate protein names: AdoMet synthase; MAT; Methionine adenosyltransferase [H]

Number of amino acids: Translated: 414; Mature: 414

Protein sequence:

>414_residues
MRIDTIRYGIDIFTIKQKGDNMEKRLFTSESVTEGHPDKICDQISDAVLDALYEQDPYSRVACETLTNTGFIMVMGEVTT
KANIDIPSIVRKTVTDIGYDSSDKGFDGNTCAVMVALDKQSADIAMGVDKALEAKDGEIDDSEGGAGDQGMMFGYATNET
PEFMPYSSALAQKLSKQLTKVRKDGTLSYLRPDGKTQVTVEYDENKKPIRLDAIVVSSQHAPEVSQEQIHEDIKKYVIDA
IVDPAMIDADTKIYINPTGRFVIGGPNGDSGLTGRKIIVDTYGGAARHGGGAFSGKDCTKVDRSAAYAARYVAKNIVAAG
LADRCEIQLSYAIGVARPTSIMVDTFGTGKLDEEKIVEIIRENFDLRPNGIIKMLDLRRPIYKQTAAYGHFGRTDLNLPW
EKLDKVDVLAKYLG

Sequences:

>Translated_414_residues
MRIDTIRYGIDIFTIKQKGDNMEKRLFTSESVTEGHPDKICDQISDAVLDALYEQDPYSRVACETLTNTGFIMVMGEVTT
KANIDIPSIVRKTVTDIGYDSSDKGFDGNTCAVMVALDKQSADIAMGVDKALEAKDGEIDDSEGGAGDQGMMFGYATNET
PEFMPYSSALAQKLSKQLTKVRKDGTLSYLRPDGKTQVTVEYDENKKPIRLDAIVVSSQHAPEVSQEQIHEDIKKYVIDA
IVDPAMIDADTKIYINPTGRFVIGGPNGDSGLTGRKIIVDTYGGAARHGGGAFSGKDCTKVDRSAAYAARYVAKNIVAAG
LADRCEIQLSYAIGVARPTSIMVDTFGTGKLDEEKIVEIIRENFDLRPNGIIKMLDLRRPIYKQTAAYGHFGRTDLNLPW
EKLDKVDVLAKYLG
>Mature_414_residues
MRIDTIRYGIDIFTIKQKGDNMEKRLFTSESVTEGHPDKICDQISDAVLDALYEQDPYSRVACETLTNTGFIMVMGEVTT
KANIDIPSIVRKTVTDIGYDSSDKGFDGNTCAVMVALDKQSADIAMGVDKALEAKDGEIDDSEGGAGDQGMMFGYATNET
PEFMPYSSALAQKLSKQLTKVRKDGTLSYLRPDGKTQVTVEYDENKKPIRLDAIVVSSQHAPEVSQEQIHEDIKKYVIDA
IVDPAMIDADTKIYINPTGRFVIGGPNGDSGLTGRKIIVDTYGGAARHGGGAFSGKDCTKVDRSAAYAARYVAKNIVAAG
LADRCEIQLSYAIGVARPTSIMVDTFGTGKLDEEKIVEIIRENFDLRPNGIIKMLDLRRPIYKQTAAYGHFGRTDLNLPW
EKLDKVDVLAKYLG

Specific function: Catalyzes the formation of S-adenosylmethionine from methionine and ATP. The overall synthetic reaction is composed of two sequential steps, AdoMet formation and the subsequent tripolyphosphate hydrolysis which occurs prior to release of AdoMet from the e

COG id: COG0192

COG function: function code H; S-adenosylmethionine synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AdoMet synthase family [H]

Homologues:

Organism=Homo sapiens, GI5174529, Length=379, Percent_Identity=57.2559366754617, Blast_Score=421, Evalue=1e-118,
Organism=Homo sapiens, GI4557737, Length=379, Percent_Identity=54.6174142480211, Blast_Score=408, Evalue=1e-114,
Organism=Escherichia coli, GI1789311, Length=388, Percent_Identity=62.8865979381443, Blast_Score=493, Evalue=1e-141,
Organism=Caenorhabditis elegans, GI17538498, Length=399, Percent_Identity=55.8897243107769, Blast_Score=427, Evalue=1e-120,
Organism=Caenorhabditis elegans, GI25145633, Length=388, Percent_Identity=56.1855670103093, Blast_Score=425, Evalue=1e-119,
Organism=Caenorhabditis elegans, GI17538494, Length=385, Percent_Identity=55.5844155844156, Blast_Score=420, Evalue=1e-118,
Organism=Caenorhabditis elegans, GI17551082, Length=382, Percent_Identity=54.9738219895288, Blast_Score=416, Evalue=1e-117,
Organism=Caenorhabditis elegans, GI32565905, Length=385, Percent_Identity=50.1298701298701, Blast_Score=357, Evalue=8e-99,
Organism=Caenorhabditis elegans, GI32565911, Length=351, Percent_Identity=53.2763532763533, Blast_Score=350, Evalue=9e-97,
Organism=Caenorhabditis elegans, GI25145635, Length=341, Percent_Identity=53.3724340175953, Blast_Score=348, Evalue=4e-96,
Organism=Caenorhabditis elegans, GI32565903, Length=308, Percent_Identity=53.2467532467532, Blast_Score=311, Evalue=5e-85,
Organism=Caenorhabditis elegans, GI32565909, Length=118, Percent_Identity=47.4576271186441, Blast_Score=117, Evalue=1e-26,
Organism=Caenorhabditis elegans, GI32565907, Length=43, Percent_Identity=72.0930232558139, Blast_Score=72, Evalue=7e-13,
Organism=Saccharomyces cerevisiae, GI6320710, Length=387, Percent_Identity=57.8811369509044, Blast_Score=434, Evalue=1e-122,
Organism=Saccharomyces cerevisiae, GI6323209, Length=380, Percent_Identity=58.9473684210526, Blast_Score=434, Evalue=1e-122,
Organism=Drosophila melanogaster, GI45552159, Length=388, Percent_Identity=54.639175257732, Blast_Score=401, Evalue=1e-112,
Organism=Drosophila melanogaster, GI24580529, Length=388, Percent_Identity=54.639175257732, Blast_Score=401, Evalue=1e-112,
Organism=Drosophila melanogaster, GI24580537, Length=388, Percent_Identity=54.639175257732, Blast_Score=401, Evalue=1e-112,
Organism=Drosophila melanogaster, GI24580533, Length=388, Percent_Identity=54.639175257732, Blast_Score=401, Evalue=1e-112,
Organism=Drosophila melanogaster, GI24580535, Length=388, Percent_Identity=54.639175257732, Blast_Score=401, Evalue=1e-112,
Organism=Drosophila melanogaster, GI24580531, Length=388, Percent_Identity=54.639175257732, Blast_Score=401, Evalue=1e-112,
Organism=Drosophila melanogaster, GI24580527, Length=386, Percent_Identity=53.8860103626943, Blast_Score=395, Evalue=1e-110,
Organism=Drosophila melanogaster, GI24580525, Length=386, Percent_Identity=53.8860103626943, Blast_Score=395, Evalue=1e-110,
Organism=Drosophila melanogaster, GI24580541, Length=374, Percent_Identity=52.6737967914438, Blast_Score=374, Evalue=1e-104,
Organism=Drosophila melanogaster, GI24580539, Length=139, Percent_Identity=51.7985611510791, Blast_Score=136, Evalue=3e-32,

Paralogues:

None

Copy number: 7,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022631
- InterPro:   IPR022630
- InterPro:   IPR022629
- InterPro:   IPR022628
- InterPro:   IPR002133
- InterPro:   IPR022636 [H]

Pfam domain/function: PF02773 S-AdoMet_synt_C; PF02772 S-AdoMet_synt_M; PF00438 S-AdoMet_synt_N [H]

EC number: =2.5.1.6 [H]

Molecular weight: Translated: 45345; Mature: 45345

Theoretical pI: Translated: 4.88; Mature: 4.88

Prosite motif: PS00376 ADOMET_SYNTHETASE_1 ; PS00377 ADOMET_SYNTHETASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIDTIRYGIDIFTIKQKGDNMEKRLFTSESVTEGHPDKICDQISDAVLDALYEQDPYSR
CCCCEEEECEEEEEEECCCCCHHHHEECCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHH
VACETLTNTGFIMVMGEVTTKANIDIPSIVRKTVTDIGYDSSDKGFDGNTCAVMVALDKQ
HHHHHHCCCCEEEEEEEECCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEEECCC
SADIAMGVDKALEAKDGEIDDSEGGAGDQGMMFGYATNETPEFMPYSSALAQKLSKQLTK
CCCEEECHHHHHCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHH
VRKDGTLSYLRPDGKTQVTVEYDENKKPIRLDAIVVSSQHAPEVSQEQIHEDIKKYVIDA
HHCCCCEEEECCCCCEEEEEEECCCCCCEEEEEEEEECCCCCCCHHHHHHHHHHHHHHHH
IVDPAMIDADTKIYINPTGRFVIGGPNGDSGLTGRKIIVDTYGGAARHGGGAFSGKDCTK
HCCCCEECCCCEEEECCCCCEEEECCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCHH
VDRSAAYAARYVAKNIVAAGLADRCEIQLSYAIGVARPTSIMVDTFGTGKLDEEKIVEII
HHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEEECCCCCEEEEECCCCCCCCHHHHHHHH
RENFDLRPNGIIKMLDLRRPIYKQTAAYGHFGRTDLNLPWEKLDKVDVLAKYLG
HHCCCCCCCCEEEEHHHCCHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHC
>Mature Secondary Structure
MRIDTIRYGIDIFTIKQKGDNMEKRLFTSESVTEGHPDKICDQISDAVLDALYEQDPYSR
CCCCEEEECEEEEEEECCCCCHHHHEECCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHH
VACETLTNTGFIMVMGEVTTKANIDIPSIVRKTVTDIGYDSSDKGFDGNTCAVMVALDKQ
HHHHHHCCCCEEEEEEEECCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEEECCC
SADIAMGVDKALEAKDGEIDDSEGGAGDQGMMFGYATNETPEFMPYSSALAQKLSKQLTK
CCCEEECHHHHHCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHH
VRKDGTLSYLRPDGKTQVTVEYDENKKPIRLDAIVVSSQHAPEVSQEQIHEDIKKYVIDA
HHCCCCEEEECCCCCEEEEEEECCCCCCEEEEEEEEECCCCCCCHHHHHHHHHHHHHHHH
IVDPAMIDADTKIYINPTGRFVIGGPNGDSGLTGRKIIVDTYGGAARHGGGAFSGKDCTK
HCCCCEECCCCEEEECCCCCEEEECCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCHH
VDRSAAYAARYVAKNIVAAGLADRCEIQLSYAIGVARPTSIMVDTFGTGKLDEEKIVEII
HHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEEECCCCCEEEEECCCCCCCCHHHHHHHH
RENFDLRPNGIIKMLDLRRPIYKQTAAYGHFGRTDLNLPWEKLDKVDVLAKYLG
HHCCCCCCCCEEEEHHHCCHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA