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The map label for this gene is dnaN [H]

Identifier: 238915978

GI number: 238915978

Start: 1658

End: 2761

Strand: Direct

Name: dnaN [H]

Synonym: EUBELI_00002

Alternate gene names: 238915978

Gene position: 1658-2761 (Clockwise)

Preceding gene: 238915977

Following gene: 238915979

Centisome position: 0.08

GC content: 30.07

Gene sequence:

>1104_bases
ATGAAATTAGTATTCACTAAATCCAACTTAAACAAAGCTGTAGGAATAGTAATGAAAGCAGTTCCTACAAGAACTACCAT
GAATATTCTGGAATGTATTCTTATTGATGCTACTACCAATGAAATAAAATTCACAGGTAATGACATGGAACTTGGAATTG
AAACAATAGTAGAAGGTGAGATTATAGAAAAAGGTAAGGTTGCCATAGATGCTAAGCTTTTTTCTGAAATTGTAAGAAAA
CTTCCTGATAATGATATTACTTTAACTACTGACTCTAATAATAATGCTCTTATTACATGTGAAAAAAGTAAGTTCAATAT
AGCAGGAAAATCCGGAGATGATTTTTCTTACCTTCCGGCTATTATTAAAGATAAGATGATTACACTTTCCCAGTTCCAGC
TTAAGGAAGTTATTAATCAGACAATATTCTCGATTGCTATTAATGATAATAATAAAATGATGACGGGTGAACTTTTTGAA
GTAAATGAAGGTACACTTAAGGTTGTAGGTCTTGATGGTCACAGAATTGCTATCCGTAATATTAAGCTCGAAGGAAGAGC
TGATGATGTAAGAGTAGTTATTCCAGGTAAGACTCTTCAGGAAATAAGCAAGATTCTTAATGCTGATGCTGAAAGCTTTG
TAAATATTTACTTTACTAACAATCATGTATTATTTGAATTTGACCAGACTCATGTTGTTTCAAGACTTATAGAGGGCGAT
TATTTTAAGATTAGTCAGATGCTTTCTAATGATTATGAAACTAAAGTATCTATTAATAAAAAAGAATTTCTTGATTCTAT
TGACAGAGCGAATCTTCTTATAAGAGAAGGAGATAAGAAGCCAATTATTATTAATATATTAAATGGTTTATTACAGGTAA
ATGTTAACTCTGCAATTGGAGCTCTTAACGAAGATATTGATATTGATAAAGAGGGTAAGGATATTATGATTGGTTTTAAT
CCTAAGTTCCTTATGGATGCATTAAGAGTTATTGATGATGAAAATGTGACTATATATTTAGTTAATCATAAATCACCTTG
CTTTATAAGAGACAAGGAAGAAAATTATATTTATCTTATACTTCCTGTTAACTTCACAGCTTAA

Upstream 100 bases:

>100_bases
GTTTTTAACATTTTAACAGTGCCTAAGACTACGGCGACTGAAATTATATATTTATATATGACACACTCCACCACATTTTT
TTTGAAAGGAAGTTATCAAC

Downstream 100 bases:

>100_bases
CAAGAGAAAGGAAAGTTATGGAAGAAATAGTTTTAAGAAATGAGTTCATCAAACTTGGACAGGCAATCAAAGCTGCCGGA
TTAGTTGAATCAGGTGTTGA

Product: DNA polymerase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 367; Mature: 367

Protein sequence:

>367_residues
MKLVFTKSNLNKAVGIVMKAVPTRTTMNILECILIDATTNEIKFTGNDMELGIETIVEGEIIEKGKVAIDAKLFSEIVRK
LPDNDITLTTDSNNNALITCEKSKFNIAGKSGDDFSYLPAIIKDKMITLSQFQLKEVINQTIFSIAINDNNKMMTGELFE
VNEGTLKVVGLDGHRIAIRNIKLEGRADDVRVVIPGKTLQEISKILNADAESFVNIYFTNNHVLFEFDQTHVVSRLIEGD
YFKISQMLSNDYETKVSINKKEFLDSIDRANLLIREGDKKPIIINILNGLLQVNVNSAIGALNEDIDIDKEGKDIMIGFN
PKFLMDALRVIDDENVTIYLVNHKSPCFIRDKEENYIYLILPVNFTA

Sequences:

>Translated_367_residues
MKLVFTKSNLNKAVGIVMKAVPTRTTMNILECILIDATTNEIKFTGNDMELGIETIVEGEIIEKGKVAIDAKLFSEIVRK
LPDNDITLTTDSNNNALITCEKSKFNIAGKSGDDFSYLPAIIKDKMITLSQFQLKEVINQTIFSIAINDNNKMMTGELFE
VNEGTLKVVGLDGHRIAIRNIKLEGRADDVRVVIPGKTLQEISKILNADAESFVNIYFTNNHVLFEFDQTHVVSRLIEGD
YFKISQMLSNDYETKVSINKKEFLDSIDRANLLIREGDKKPIIINILNGLLQVNVNSAIGALNEDIDIDKEGKDIMIGFN
PKFLMDALRVIDDENVTIYLVNHKSPCFIRDKEENYIYLILPVNFTA
>Mature_367_residues
MKLVFTKSNLNKAVGIVMKAVPTRTTMNILECILIDATTNEIKFTGNDMELGIETIVEGEIIEKGKVAIDAKLFSEIVRK
LPDNDITLTTDSNNNALITCEKSKFNIAGKSGDDFSYLPAIIKDKMITLSQFQLKEVINQTIFSIAINDNNKMMTGELFE
VNEGTLKVVGLDGHRIAIRNIKLEGRADDVRVVIPGKTLQEISKILNADAESFVNIYFTNNHVLFEFDQTHVVSRLIEGD
YFKISQMLSNDYETKVSINKKEFLDSIDRANLLIREGDKKPIIINILNGLLQVNVNSAIGALNEDIDIDKEGKDIMIGFN
PKFLMDALRVIDDENVTIYLVNHKSPCFIRDKEENYIYLILPVNFTA

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity. The beta chain is required for initiation of replication once it is clamped

COG id: COG0592

COG function: function code L; DNA polymerase sliding clamp subunit (PCNA homolog)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1790136, Length=370, Percent_Identity=22.972972972973, Blast_Score=106, Evalue=2e-24,

Paralogues:

None

Copy number: 5954 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001001
- InterPro:   IPR022635
- InterPro:   IPR022637
- InterPro:   IPR022634 [H]

Pfam domain/function: PF00712 DNA_pol3_beta; PF02767 DNA_pol3_beta_2; PF02768 DNA_pol3_beta_3 [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 41289; Mature: 41289

Theoretical pI: Translated: 4.76; Mature: 4.76

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLVFTKSNLNKAVGIVMKAVPTRTTMNILECILIDATTNEIKFTGNDMELGIETIVEGE
CEEEEECCCCCHHHHHHEECCCCCHHHHEEEEEEEECCCCEEEEECCCCEECHHHEECCC
IIEKGKVAIDAKLFSEIVRKLPDNDITLTTDSNNNALITCEKSKFNIAGKSGDDFSYLPA
EECCCCEEEEHHHHHHHHHHCCCCCEEEEECCCCCEEEEEECCEEEEECCCCCCCHHHHH
IIKDKMITLSQFQLKEVINQTIFSIAINDNNKMMTGELFEVNEGTLKVVGLDGHRIAIRN
HHHCCEEEHHHHHHHHHHCCEEEEEEECCCCEEEEEEEEEECCCEEEEEEECCCEEEEEE
IKLEGRADDVRVVIPGKTLQEISKILNADAESFVNIYFTNNHVLFEFDQTHVVSRLIEGD
EEEECCCCCEEEEECCHHHHHHHHHHCCCCCCEEEEEEECCEEEEEECHHHHHHHHHCCC
YFKISQMLSNDYETKVSINKKEFLDSIDRANLLIREGDKKPIIINILNGLLQVNVNSAIG
HHHHHHHHCCCCCEEEEECHHHHHHHHHHCEEEEEECCCCCEEEEEECCCEEEECCHHHC
ALNEDIDIDKEGKDIMIGFNPKFLMDALRVIDDENVTIYLVNHKSPCFIRDKEENYIYLI
CCCCCCCCCCCCCEEEEECCHHHHHHHHHHHCCCCEEEEEEECCCCEEEEECCCCEEEEE
LPVNFTA
EEEEECC
>Mature Secondary Structure
MKLVFTKSNLNKAVGIVMKAVPTRTTMNILECILIDATTNEIKFTGNDMELGIETIVEGE
CEEEEECCCCCHHHHHHEECCCCCHHHHEEEEEEEECCCCEEEEECCCCEECHHHEECCC
IIEKGKVAIDAKLFSEIVRKLPDNDITLTTDSNNNALITCEKSKFNIAGKSGDDFSYLPA
EECCCCEEEEHHHHHHHHHHCCCCCEEEEECCCCCEEEEEECCEEEEECCCCCCCHHHHH
IIKDKMITLSQFQLKEVINQTIFSIAINDNNKMMTGELFEVNEGTLKVVGLDGHRIAIRN
HHHCCEEEHHHHHHHHHHCCEEEEEEECCCCEEEEEEEEEECCCEEEEEEECCCEEEEEE
IKLEGRADDVRVVIPGKTLQEISKILNADAESFVNIYFTNNHVLFEFDQTHVVSRLIEGD
EEEECCCCCEEEEECCHHHHHHHHHHCCCCCCEEEEEEECCEEEEEECHHHHHHHHHCCC
YFKISQMLSNDYETKVSINKKEFLDSIDRANLLIREGDKKPIIINILNGLLQVNVNSAIG
HHHHHHHHCCCCCEEEEECHHHHHHHHHHCEEEEEECCCCCEEEEEECCCEEEECCHHHC
ALNEDIDIDKEGKDIMIGFNPKFLMDALRVIDDENVTIYLVNHKSPCFIRDKEENYIYLI
CCCCCCCCCCCCCEEEEECCHHHHHHHHHHHCCCCEEEEEEECCCCEEEEECCCCEEEEE
LPVNFTA
EEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA