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The map label for this gene is pcaQ [H]

Identifier: 238897155

GI number: 238897155

Start: 5155029

End: 5156255

Strand: Direct

Name: pcaQ [H]

Synonym: KP1_5419

Alternate gene names: 238897155

Gene position: 5155029-5156255 (Clockwise)

Preceding gene: 238897150

Following gene: 238897160

Centisome position: 98.22

GC content: 62.02

Gene sequence:

>1227_bases
ATGCTAAAGAGAAAGCACGATAAAATTATAAATATCATGCAGCTGCGCTTTTTCTGTCAGGTCGCGCTGCGCGGTAGCGT
CTCACGCGCGGCCGATGACTTATTCCGCACGCAGTCAGCGATAACCCGGGCTATTCGCGATCTTGAAGCCGCGTTGAACG
TCACGCTGTTCGAACGCCATTACAGCGGCATGGTGCCCACCGAGTATGGAAAGTGCATCCTGCCGCGCGCCCGGCGGGCG
ATTGACGACCTGCAGGCCATCCCGGCGCTGCTGCAGAAGCACCATACCCGCAGCAGCGGCCCGCTGGCGGACGCCGGCTG
GCTGTTCAATACCCGCCGCTTAGCCATTTTTATTCAGCTCTATCACGTCAACCATACCCAAACCGTCGCGCAACAGTTGG
GGATCACTCAGCCGGCGGTCAGTGCCGCGCTGAAGGTCCTGGAAAAAGGAGCGGATTCGGCGCTATTTCGCCGGACGCCA
GAGGGGGTGCGTCCCACGCCTGCCGCAGAGCTACTCTATCCCCCGGTGAGCAGAGCGCTCAACGAGCTGGAAAACATCTG
GAGCGATCTCGCTGCCCGTCGCGGGGTGCTGGAGGGCACCGTGCGCATTGGCGCCCTGCCGCTGAGCCGGACCCGGCTAC
TGCCCTCCGCCATCGCCGCCTTTCTGGCCCAGCACCCGGGCATCACCCTCATGACCAATGAAAGCCCGTATGAGTCGCTG
GTCGCCGATATGCGGGCAGGTAATATTGATTTTATTATCGGCGCCCTGCGCCAGGATGAAGATCTCCCGGACCTCTGTAG
CGAAGCGTTATTCGAAGAGGATATGTTGATTTTACTGCGTAATAACCACCCGCTGCTTCGCCATCCGGACCCGCGCAGCC
AGCTGGCGACAGCGCAATGGGTGCTGCCGCGCGCCAACGCGCCGGCGCGCAATTTGCTGGATAAGGCGTTTGTCACCCTG
GGGCTGCCGCTGCCCCAGCCGACGGTAGAGACCGGGGATGCGGCGATGGTGCGAGGATTGCTGCAGGGCTCCGACATGCT
GGCGGCGGTCTCCGCCAGCCAGATGCGTTTTGAAACTGACAATGGGCTGCTCAGCGTGTTGCCGGTTCCCCTGCCCGATA
CCACCCGGCGTATCGGGCTGACGTTTCGCGCCGGCAGCCTGCCCTCCCCGGCGACGCAGGCGCTGTTGCGTTTTATTTAT
CAGCAGGTTCAGGACGGGGCCGTTTAA

Upstream 100 bases:

>100_bases
CTTGTCATGTCCCGTCGTGGGGAGGTAATTCGTTAACTTGCGAAGGCTATGATTTTTATTTATGTTGTCGTCTCACTCAC
TTTTTCAGGCCTGACGAACA

Downstream 100 bases:

>100_bases
CGTTTACCCGCCACCGAATAGGTTACCTGCTGGTTGCGCGTTTGCTGAAACGCCTCCAGCGTCTGCCCGCGCATCGCGGC
GTAAATATGCAGGTTGGATA

Product: putative LysR-family transcriptional regulator

Products: NA

Alternate protein names: Pca operon transcriptional activator [H]

Number of amino acids: Translated: 408; Mature: 408

Protein sequence:

>408_residues
MLKRKHDKIINIMQLRFFCQVALRGSVSRAADDLFRTQSAITRAIRDLEAALNVTLFERHYSGMVPTEYGKCILPRARRA
IDDLQAIPALLQKHHTRSSGPLADAGWLFNTRRLAIFIQLYHVNHTQTVAQQLGITQPAVSAALKVLEKGADSALFRRTP
EGVRPTPAAELLYPPVSRALNELENIWSDLAARRGVLEGTVRIGALPLSRTRLLPSAIAAFLAQHPGITLMTNESPYESL
VADMRAGNIDFIIGALRQDEDLPDLCSEALFEEDMLILLRNNHPLLRHPDPRSQLATAQWVLPRANAPARNLLDKAFVTL
GLPLPQPTVETGDAAMVRGLLQGSDMLAAVSASQMRFETDNGLLSVLPVPLPDTTRRIGLTFRAGSLPSPATQALLRFIY
QQVQDGAV

Sequences:

>Translated_408_residues
MLKRKHDKIINIMQLRFFCQVALRGSVSRAADDLFRTQSAITRAIRDLEAALNVTLFERHYSGMVPTEYGKCILPRARRA
IDDLQAIPALLQKHHTRSSGPLADAGWLFNTRRLAIFIQLYHVNHTQTVAQQLGITQPAVSAALKVLEKGADSALFRRTP
EGVRPTPAAELLYPPVSRALNELENIWSDLAARRGVLEGTVRIGALPLSRTRLLPSAIAAFLAQHPGITLMTNESPYESL
VADMRAGNIDFIIGALRQDEDLPDLCSEALFEEDMLILLRNNHPLLRHPDPRSQLATAQWVLPRANAPARNLLDKAFVTL
GLPLPQPTVETGDAAMVRGLLQGSDMLAAVSASQMRFETDNGLLSVLPVPLPDTTRRIGLTFRAGSLPSPATQALLRFIY
QQVQDGAV
>Mature_408_residues
MLKRKHDKIINIMQLRFFCQVALRGSVSRAADDLFRTQSAITRAIRDLEAALNVTLFERHYSGMVPTEYGKCILPRARRA
IDDLQAIPALLQKHHTRSSGPLADAGWLFNTRRLAIFIQLYHVNHTQTVAQQLGITQPAVSAALKVLEKGADSALFRRTP
EGVRPTPAAELLYPPVSRALNELENIWSDLAARRGVLEGTVRIGALPLSRTRLLPSAIAAFLAQHPGITLMTNESPYESL
VADMRAGNIDFIIGALRQDEDLPDLCSEALFEEDMLILLRNNHPLLRHPDPRSQLATAQWVLPRANAPARNLLDKAFVTL
GLPLPQPTVETGDAAMVRGLLQGSDMLAAVSASQMRFETDNGLLSVLPVPLPDTTRRIGLTFRAGSLPSPATQALLRFIY
QQVQDGAV

Specific function: Activates transcription of the pcaDCHGB operon for the catabolism of the phenolic compound protocatechuate [H]

COG id: COG0583

COG function: function code K; Transcriptional regulator

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI87081904, Length=291, Percent_Identity=28.1786941580756, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI1787601, Length=190, Percent_Identity=31.0526315789474, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI1789506, Length=240, Percent_Identity=25.4166666666667, Blast_Score=76, Evalue=3e-15,
Organism=Escherichia coli, GI1788481, Length=292, Percent_Identity=26.7123287671233, Blast_Score=72, Evalue=5e-14,
Organism=Escherichia coli, GI1790399, Length=284, Percent_Identity=23.943661971831, Blast_Score=65, Evalue=9e-12,
Organism=Escherichia coli, GI157672245, Length=290, Percent_Identity=26.8965517241379, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1788748, Length=215, Percent_Identity=26.046511627907, Blast_Score=62, Evalue=6e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR012787
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 44924; Mature: 44924

Theoretical pI: Translated: 9.44; Mature: 9.44

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKRKHDKIINIMQLRFFCQVALRGSVSRAADDLFRTQSAITRAIRDLEAALNVTLFERH
CCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YSGMVPTEYGKCILPRARRAIDDLQAIPALLQKHHTRSSGPLADAGWLFNTRRLAIFIQL
HCCCCCCCCCCEECHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHCCCCEEEEEEEE
YHVNHTQTVAQQLGITQPAVSAALKVLEKGADSALFRRTPEGVRPTPAAELLYPPVSRAL
EECCHHHHHHHHHCCCCHHHHHHHHHHHCCCCHHHHHCCCCCCCCCCHHHHHCCHHHHHH
NELENIWSDLAARRGVLEGTVRIGALPLSRTRLLPSAIAAFLAQHPGITLMTNESPYESL
HHHHHHHHHHHHHCCHHHCCEEEECCCCHHHHHHHHHHHHHHHHCCCEEEEECCCHHHHH
VADMRAGNIDFIIGALRQDEDLPDLCSEALFEEDMLILLRNNHPLLRHPDPRSQLATAQW
HHHHCCCCHHHEEECHHCCCCCHHHHHHHHHCCCEEEEEECCCCCCCCCCCHHHHHHHHH
VLPRANAPARNLLDKAFVTLGLPLPQPTVETGDAAMVRGLLQGSDMLAAVSASQMRFETD
CCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHEEECC
NGLLSVLPVPLPDTTRRIGLTFRAGSLPSPATQALLRFIYQQVQDGAV
CCEEEEECCCCCCCCHHEEEEEECCCCCCHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MLKRKHDKIINIMQLRFFCQVALRGSVSRAADDLFRTQSAITRAIRDLEAALNVTLFERH
CCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YSGMVPTEYGKCILPRARRAIDDLQAIPALLQKHHTRSSGPLADAGWLFNTRRLAIFIQL
HCCCCCCCCCCEECHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHCCCCEEEEEEEE
YHVNHTQTVAQQLGITQPAVSAALKVLEKGADSALFRRTPEGVRPTPAAELLYPPVSRAL
EECCHHHHHHHHHCCCCHHHHHHHHHHHCCCCHHHHHCCCCCCCCCCHHHHHCCHHHHHH
NELENIWSDLAARRGVLEGTVRIGALPLSRTRLLPSAIAAFLAQHPGITLMTNESPYESL
HHHHHHHHHHHHHCCHHHCCEEEECCCCHHHHHHHHHHHHHHHHCCCEEEEECCCHHHHH
VADMRAGNIDFIIGALRQDEDLPDLCSEALFEEDMLILLRNNHPLLRHPDPRSQLATAQW
HHHHCCCCHHHEEECHHCCCCCHHHHHHHHHCCCEEEEEECCCCCCCCCCCHHHHHHHHH
VLPRANAPARNLLDKAFVTLGLPLPQPTVETGDAAMVRGLLQGSDMLAAVSASQMRFETD
CCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHEEECC
NGLLSVLPVPLPDTTRRIGLTFRAGSLPSPATQALLRFIYQQVQDGAV
CCEEEEECCCCCCCCHHEEEEEECCCCCCHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11743193; 11743194 [H]