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The map label for this gene is mexA [H]

Identifier: 238897143

GI number: 238897143

Start: 5140713

End: 5141837

Strand: Direct

Name: mexA [H]

Synonym: KP1_5406

Alternate gene names: 238897143

Gene position: 5140713-5141837 (Clockwise)

Preceding gene: 238897141

Following gene: 238897144

Centisome position: 97.95

GC content: 63.29

Gene sequence:

>1125_bases
ATGAAATACATCATAGCTCCCATCGCGACAGCATTATTTCTCCTTAGCGGATGCGATAATGCGCAGACATCTGCTCCGCA
GCAACCCACGCCGGAAGTGGGCGTGGTCACCCTGCAAAGCCAGCCGGTGCCGGTAGTCAGCCAGCTCACCGGCCGCACCA
CCGCCTCGCTCAGCGCCGAAGTTCGCCCGCAGGTGGGCGGCATCATCCAGAAACGCCTGTTTACCGAAGGCGATATGGTG
AAGGCCGGGCAGGCCCTTTACCAGATCGATCCCTCCAGCTATCGCGCGGCCTGGAACGAAGCGGCGGCGGCGCTGAAACA
GGCCCAGGCGCTGGTGGTCTCCGATTGCCAGAAGGCCCAGCGCTACGCGTCGCTGGTCAGGGATAACGGCGTTTCCCGCC
AGGATGCTGACGACGCCGCCGCCACCTGCGCCCAGGACAAGGCCAGCGTGGAATCGAAGAAAGCGGCGCTGGAGAGCGCG
CGCATTAACCTGAACTGGACCACCGTCACCGCGCCGATTGCCGGGCGCATCGGCATCTCGTCGGTTACGCCGGGGGCGCT
GGTCTCCGCCGATCAGGATACCGCGCTGGCGACGATCCGCGGGCTCGACACCATGTATGTTGATCTGACGCGATCCAGCG
TCGATCTCCTGCGTTTACGCAAGCAAAGCCTGGCCAGCAACAGCGACACCCTCAGCGTGACGTTAACCCTGGAAGATGGC
AGTACCTATCCCGAAAAAGGGCGTCTGGCGCTCACCGAAGTCGCCGTCGATGAATCTACCGGCTCGGTAACCCTGCGCGC
TATCTTCCCCAACCCGCAGCACGTCCTGCTACCGGGGATGTTCGTCCGCGCGCGCATCGATGAAGGCATCATGAACGACG
CGATCCTCGCCCCGCAGCAGGGGATCACCCGCGATGCCAAAGGCGACGCCACGGCGCTGGTGGTCGATGCCGCCAACAAA
GTTGAGCAGCGTACCGTGGAAACCGGCGACACCTACGGCGATAAATGGCTGGTGCTGAGCGGCCTGAAAGCGGGCGACAA
GCTGATTGTCGAAGGCACCGGTAAAGTGGCCCCCGGACAGACAGTGAAGGCCGTCGCCGTCAACAATAACGGAGGCAACG
CCTGA

Upstream 100 bases:

>100_bases
TTTTTTGTCATTTTCGCTCACCCGAAAATGCCTCAGCGCCCAACCTATTCCCTCGTTTTTAAATAAACGGGGCGGACGTC
TTATATCTTGAGAAAATATA

Downstream 100 bases:

>100_bases
TGTTCTCCCGTTTCTTCGTGCGTCGCCCGGTATTTGCCTGGGTGATCGCCATTCTGATTATGCTCGCCGGGGTGCTGGCG
ATCCGCACGCTGCCGGTAGG

Product: putative secretion protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 374; Mature: 374

Protein sequence:

>374_residues
MKYIIAPIATALFLLSGCDNAQTSAPQQPTPEVGVVTLQSQPVPVVSQLTGRTTASLSAEVRPQVGGIIQKRLFTEGDMV
KAGQALYQIDPSSYRAAWNEAAAALKQAQALVVSDCQKAQRYASLVRDNGVSRQDADDAAATCAQDKASVESKKAALESA
RINLNWTTVTAPIAGRIGISSVTPGALVSADQDTALATIRGLDTMYVDLTRSSVDLLRLRKQSLASNSDTLSVTLTLEDG
STYPEKGRLALTEVAVDESTGSVTLRAIFPNPQHVLLPGMFVRARIDEGIMNDAILAPQQGITRDAKGDATALVVDAANK
VEQRTVETGDTYGDKWLVLSGLKAGDKLIVEGTGKVAPGQTVKAVAVNNNGGNA

Sequences:

>Translated_374_residues
MKYIIAPIATALFLLSGCDNAQTSAPQQPTPEVGVVTLQSQPVPVVSQLTGRTTASLSAEVRPQVGGIIQKRLFTEGDMV
KAGQALYQIDPSSYRAAWNEAAAALKQAQALVVSDCQKAQRYASLVRDNGVSRQDADDAAATCAQDKASVESKKAALESA
RINLNWTTVTAPIAGRIGISSVTPGALVSADQDTALATIRGLDTMYVDLTRSSVDLLRLRKQSLASNSDTLSVTLTLEDG
STYPEKGRLALTEVAVDESTGSVTLRAIFPNPQHVLLPGMFVRARIDEGIMNDAILAPQQGITRDAKGDATALVVDAANK
VEQRTVETGDTYGDKWLVLSGLKAGDKLIVEGTGKVAPGQTVKAVAVNNNGGNA
>Mature_374_residues
MKYIIAPIATALFLLSGCDNAQTSAPQQPTPEVGVVTLQSQPVPVVSQLTGRTTASLSAEVRPQVGGIIQKRLFTEGDMV
KAGQALYQIDPSSYRAAWNEAAAALKQAQALVVSDCQKAQRYASLVRDNGVSRQDADDAAATCAQDKASVESKKAALESA
RINLNWTTVTAPIAGRIGISSVTPGALVSADQDTALATIRGLDTMYVDLTRSSVDLLRLRKQSLASNSDTLSVTLTLEDG
STYPEKGRLALTEVAVDESTGSVTLRAIFPNPQHVLLPGMFVRARIDEGIMNDAILAPQQGITRDAKGDATALVVDAANK
VEQRTVETGDTYGDKWLVLSGLKAGDKLIVEGTGKVAPGQTVKAVAVNNNGGNA

Specific function: The ability to export antibiotics and solvents is dramatically decreased in the presence of the proton conductor carbonyl cyanide m-chlorophenylhydrazone (CCCP), showing that an energized inner membrane is required for efflux. It is thought that the mexB

COG id: COG0845

COG function: function code M; Membrane-fusion protein

Gene ontology:

Cell location: Cell inner membrane; Lipid-anchor. Note=The membrane anchor is not necessary for antibiotic efflux as the protein is functional when targeted to the periplasm by a foreign signal peptide [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI1789665, Length=369, Percent_Identity=48.780487804878, Blast_Score=337, Evalue=1e-93,
Organism=Escherichia coli, GI1786668, Length=377, Percent_Identity=49.3368700265252, Blast_Score=334, Evalue=7e-93,
Organism=Escherichia coli, GI1789929, Length=377, Percent_Identity=44.8275862068966, Blast_Score=303, Evalue=2e-83,
Organism=Escherichia coli, GI87082036, Length=337, Percent_Identity=27.5964391691395, Blast_Score=125, Evalue=5e-30,
Organism=Escherichia coli, GI87081796, Length=331, Percent_Identity=25.9818731117825, Blast_Score=63, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006143 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 39288; Mature: 39288

Theoretical pI: Translated: 5.24; Mature: 5.24

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKYIIAPIATALFLLSGCDNAQTSAPQQPTPEVGVVTLQSQPVPVVSQLTGRTTASLSAE
CCEEHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEECCCCCCHHHHHCCCCEEECCEE
VRPQVGGIIQKRLFTEGDMVKAGQALYQIDPSSYRAAWNEAAAALKQAQALVVSDCQKAQ
CCCHHHHHHHHHHCCCCCHHHCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RYASLVRDNGVSRQDADDAAATCAQDKASVESKKAALESARINLNWTTVTAPIAGRIGIS
HHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHEEEEEEEEEEEECCCCCCCCC
SVTPGALVSADQDTALATIRGLDTMYVDLTRSSVDLLRLRKQSLASNSDTLSVTLTLEDG
CCCCCCEEECCCCCCEEHHHCCCEEEEEEECCHHHHHHHHHHHHCCCCCEEEEEEEEECC
STYPEKGRLALTEVAVDESTGSVTLRAIFPNPQHVLLPGMFVRARIDEGIMNDAILAPQQ
CCCCCCCCEEEEEEEEECCCCCEEEEEECCCCCEEECCCEEEEEECCCCCCCCCEECCCC
GITRDAKGDATALVVDAANKVEQRTVETGDTYGDKWLVLSGLKAGDKLIVEGTGKVAPGQ
CCCCCCCCCCEEEEEECCHHHHHHHHHCCCCCCCCEEEEECCCCCCEEEEEECCCCCCCC
TVKAVAVNNNGGNA
EEEEEEEECCCCCC
>Mature Secondary Structure
MKYIIAPIATALFLLSGCDNAQTSAPQQPTPEVGVVTLQSQPVPVVSQLTGRTTASLSAE
CCEEHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEECCCCCCHHHHHCCCCEEECCEE
VRPQVGGIIQKRLFTEGDMVKAGQALYQIDPSSYRAAWNEAAAALKQAQALVVSDCQKAQ
CCCHHHHHHHHHHCCCCCHHHCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RYASLVRDNGVSRQDADDAAATCAQDKASVESKKAALESARINLNWTTVTAPIAGRIGIS
HHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHEEEEEEEEEEEECCCCCCCCC
SVTPGALVSADQDTALATIRGLDTMYVDLTRSSVDLLRLRKQSLASNSDTLSVTLTLEDG
CCCCCCEEECCCCCCEEHHHCCCEEEEEEECCHHHHHHHHHHHHCCCCCEEEEEEEEECC
STYPEKGRLALTEVAVDESTGSVTLRAIFPNPQHVLLPGMFVRARIDEGIMNDAILAPQQ
CCCCCCCCEEEEEEEEECCCCCEEEEEECCCCCEEECCCEEEEEECCCCCCCCCEECCCC
GITRDAKGDATALVVDAANKVEQRTVETGDTYGDKWLVLSGLKAGDKLIVEGTGKVAPGQ
CCCCCCCCCCEEEEEECCHHHHHHHHHCCCCCCCCEEEEECCCCCCEEEEEECCCCCCCC
TVKAVAVNNNGGNA
EEEEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7968531; 10984043; 9989496; 8226684; 8540696; 9603892; 10671490 [H]