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The map label for this gene is glpF [H]

Identifier: 238897112

GI number: 238897112

Start: 5106415

End: 5107266

Strand: Direct

Name: glpF [H]

Synonym: KP1_5368

Alternate gene names: 238897112

Gene position: 5106415-5107266 (Clockwise)

Preceding gene: 238897107

Following gene: 238897113

Centisome position: 97.29

GC content: 59.15

Gene sequence:

>852_bases
ATGAGCCAAACATCAACCTTAAAAGGCCAGTGCATCGCAGAGTTCCTCGGTACCGGGTTGTTGATCTTTTTCGGCGTTGG
GTGCGTGGCTGCGCTCAAGGTCGCGGGAGCCAGCTTCGGACAATGGGAAATCAGCATCATCTGGGGTCTGGGCGTCGCCA
TGGCGATCTACCTGACCGCCGGGGTCTCCGGTGCGCACCTTAACCCAGCGGTGACTATCGCGCTGTGGCTGTTCGCCTGC
TTCGAAGGCCGCAAAGTGGTTCCTTTTATTATTTCGCAATTCGCTGGCGCCTTTTGCGCTGCGGCATTAGTTTACGGGCT
TTACTACAATCTTTTCCTCGATTATGAAACCACCCACCATATGATTCGCGGCAGCGTGGAAAGCCTCGATCTGGCCGGCA
TTTTCTCCACTTACCCGAACCCGCACATCAATTTTGTGCAGGCCTTCGCGGTAGAGATGGTGATTACCGCTATCCTGATG
GGCGTCATCCTGGCGCTGACCGACGATGGCAACGGCATACCGCGCGGCCCGCTGGCCCCTCTGCTGATTGGCCTGCTGAT
TGCGGTGATTGGCGCCTCCATGGGACCGCTGACCGGCTTCGCCATGAACCCGGCGCGTGACATCGGCCCGAAAGCCTTCG
CCTGGCTGGCGGGGTGGGGTGACGTCGCCTTCACCGGCGGCAAAGATATTCCTTATTTCCTGGTGCCGCTGTGCGCACCG
GTGGTCGGCGCGGCGCTGGGCGCATTCAGCTATCGTAAGCTGATTGGCCGTCACCTGCCTTGCGACACCTGCGTGGAGGA
AGAGCAACAGAGCCCCTCCTCTTCCACCGCTCAACATAAAGCTTCGCTGTAA

Upstream 100 bases:

>100_bases
TTGCTCGTTAACGATAAATTAACACTATGCCTACATGGCAATATGGTCGTCTGTGACCACCCTACATACAATAATCATCA
CCACGCTTCAGGATTCGATT

Downstream 100 bases:

>100_bases
TCTGACTACGGGACACCGACTATGACCGACAAAAAATATATCGTTGCGCTTGACCAGGGCACTACCAGCTCCCGGGCCGT
TGTGATGGATCATGATGCCA

Product: MIP family channel protein

Products: glycerol [Cytoplasm] [C]

Alternate protein names: Aquaglyceroporin [H]

Number of amino acids: Translated: 283; Mature: 282

Protein sequence:

>283_residues
MSQTSTLKGQCIAEFLGTGLLIFFGVGCVAALKVAGASFGQWEISIIWGLGVAMAIYLTAGVSGAHLNPAVTIALWLFAC
FEGRKVVPFIISQFAGAFCAAALVYGLYYNLFLDYETTHHMIRGSVESLDLAGIFSTYPNPHINFVQAFAVEMVITAILM
GVILALTDDGNGIPRGPLAPLLIGLLIAVIGASMGPLTGFAMNPARDIGPKAFAWLAGWGDVAFTGGKDIPYFLVPLCAP
VVGAALGAFSYRKLIGRHLPCDTCVEEEQQSPSSSTAQHKASL

Sequences:

>Translated_283_residues
MSQTSTLKGQCIAEFLGTGLLIFFGVGCVAALKVAGASFGQWEISIIWGLGVAMAIYLTAGVSGAHLNPAVTIALWLFAC
FEGRKVVPFIISQFAGAFCAAALVYGLYYNLFLDYETTHHMIRGSVESLDLAGIFSTYPNPHINFVQAFAVEMVITAILM
GVILALTDDGNGIPRGPLAPLLIGLLIAVIGASMGPLTGFAMNPARDIGPKAFAWLAGWGDVAFTGGKDIPYFLVPLCAP
VVGAALGAFSYRKLIGRHLPCDTCVEEEQQSPSSSTAQHKASL
>Mature_282_residues
SQTSTLKGQCIAEFLGTGLLIFFGVGCVAALKVAGASFGQWEISIIWGLGVAMAIYLTAGVSGAHLNPAVTIALWLFACF
EGRKVVPFIISQFAGAFCAAALVYGLYYNLFLDYETTHHMIRGSVESLDLAGIFSTYPNPHINFVQAFAVEMVITAILMG
VILALTDDGNGIPRGPLAPLLIGLLIAVIGASMGPLTGFAMNPARDIGPKAFAWLAGWGDVAFTGGKDIPYFLVPLCAPV
VGAALGAFSYRKLIGRHLPCDTCVEEEQQSPSSSTAQHKASL

Specific function: Transporter of glycerol across the cytoplasmic membrane, with limited permeability to water and small uncharged compounds such as polyols [H]

COG id: COG0580

COG function: function code G; Glycerol uptake facilitator and related permeases (Major Intrinsic Protein Family)

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MIP/aquaporin (TC 1.A.8) family [H]

Homologues:

Organism=Homo sapiens, GI157266307, Length=273, Percent_Identity=40.6593406593407, Blast_Score=182, Evalue=3e-46,
Organism=Homo sapiens, GI22538420, Length=280, Percent_Identity=37.1428571428571, Blast_Score=167, Evalue=1e-41,
Organism=Homo sapiens, GI4826645, Length=276, Percent_Identity=37.3188405797101, Blast_Score=166, Evalue=3e-41,
Organism=Homo sapiens, GI4502187, Length=261, Percent_Identity=34.8659003831418, Blast_Score=155, Evalue=5e-38,
Organism=Homo sapiens, GI310133356, Length=217, Percent_Identity=31.7972350230415, Blast_Score=102, Evalue=3e-22,
Organism=Homo sapiens, GI310114181, Length=217, Percent_Identity=31.7972350230415, Blast_Score=102, Evalue=3e-22,
Organism=Homo sapiens, GI86792455, Length=203, Percent_Identity=35.9605911330049, Blast_Score=73, Evalue=2e-13,
Organism=Homo sapiens, GI4502179, Length=283, Percent_Identity=32.1554770318021, Blast_Score=71, Evalue=1e-12,
Organism=Homo sapiens, GI37694062, Length=203, Percent_Identity=31.0344827586207, Blast_Score=67, Evalue=3e-11,
Organism=Escherichia coli, GI1790362, Length=283, Percent_Identity=86.9257950530035, Blast_Score=506, Evalue=1e-145,
Organism=Escherichia coli, GI1787101, Length=207, Percent_Identity=31.8840579710145, Blast_Score=64, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI71994009, Length=267, Percent_Identity=33.7078651685393, Blast_Score=146, Evalue=1e-35,
Organism=Caenorhabditis elegans, GI32564052, Length=283, Percent_Identity=33.5689045936396, Blast_Score=138, Evalue=3e-33,
Organism=Caenorhabditis elegans, GI17533613, Length=283, Percent_Identity=33.5689045936396, Blast_Score=138, Evalue=3e-33,
Organism=Caenorhabditis elegans, GI71992966, Length=258, Percent_Identity=32.5581395348837, Blast_Score=131, Evalue=4e-31,
Organism=Caenorhabditis elegans, GI17544068, Length=265, Percent_Identity=32.8301886792453, Blast_Score=127, Evalue=6e-30,
Organism=Caenorhabditis elegans, GI17531429, Length=259, Percent_Identity=32.8185328185328, Blast_Score=124, Evalue=6e-29,
Organism=Caenorhabditis elegans, GI17531431, Length=259, Percent_Identity=32.8185328185328, Blast_Score=124, Evalue=6e-29,
Organism=Caenorhabditis elegans, GI71992961, Length=258, Percent_Identity=28.6821705426357, Blast_Score=98, Evalue=6e-21,
Organism=Caenorhabditis elegans, GI17558372, Length=258, Percent_Identity=25.5813953488372, Blast_Score=66, Evalue=3e-11,
Organism=Saccharomyces cerevisiae, GI6321054, Length=216, Percent_Identity=37.037037037037, Blast_Score=147, Evalue=2e-36,
Organism=Saccharomyces cerevisiae, GI6322985, Length=312, Percent_Identity=27.2435897435897, Blast_Score=97, Evalue=4e-21,
Organism=Drosophila melanogaster, GI45550503, Length=263, Percent_Identity=25.4752851711027, Blast_Score=78, Evalue=5e-15,
Organism=Drosophila melanogaster, GI24762344, Length=249, Percent_Identity=29.718875502008, Blast_Score=78, Evalue=7e-15,
Organism=Drosophila melanogaster, GI24762346, Length=249, Percent_Identity=29.718875502008, Blast_Score=78, Evalue=9e-15,
Organism=Drosophila melanogaster, GI24762348, Length=249, Percent_Identity=29.718875502008, Blast_Score=78, Evalue=9e-15,
Organism=Drosophila melanogaster, GI20130305, Length=249, Percent_Identity=29.718875502008, Blast_Score=78, Evalue=9e-15,
Organism=Drosophila melanogaster, GI24652747, Length=210, Percent_Identity=31.9047619047619, Blast_Score=70, Evalue=1e-12,
Organism=Drosophila melanogaster, GI45551084, Length=210, Percent_Identity=31.9047619047619, Blast_Score=70, Evalue=1e-12,
Organism=Drosophila melanogaster, GI24762342, Length=215, Percent_Identity=28.3720930232558, Blast_Score=67, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012269
- InterPro:   IPR000425
- InterPro:   IPR022357 [H]

Pfam domain/function: PF00230 MIP [H]

EC number: NA

Molecular weight: Translated: 29808; Mature: 29677

Theoretical pI: Translated: 6.22; Mature: 6.22

Prosite motif: PS00221 MIP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQTSTLKGQCIAEFLGTGLLIFFGVGCVAALKVAGASFGQWEISIIWGLGVAMAIYLTA
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEHHHHHHHHHHHHHC
GVSGAHLNPAVTIALWLFACFEGRKVVPFIISQFAGAFCAAALVYGLYYNLFLDYETTHH
CCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHH
MIRGSVESLDLAGIFSTYPNPHINFVQAFAVEMVITAILMGVILALTDDGNGIPRGPLAP
HHHCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHH
LLIGLLIAVIGASMGPLTGFAMNPARDIGPKAFAWLAGWGDVAFTGGKDIPYFLVPLCAP
HHHHHHHHHHCCCCCCCCCCCCCCHHHCCHHHHHHHHCCCCEEEECCCCCHHHHHHHHHH
VVGAALGAFSYRKLIGRHLPCDTCVEEEQQSPSSSTAQHKASL
HHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCHHHHHHCCC
>Mature Secondary Structure 
SQTSTLKGQCIAEFLGTGLLIFFGVGCVAALKVAGASFGQWEISIIWGLGVAMAIYLTA
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEHHHHHHHHHHHHHC
GVSGAHLNPAVTIALWLFACFEGRKVVPFIISQFAGAFCAAALVYGLYYNLFLDYETTHH
CCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHH
MIRGSVESLDLAGIFSTYPNPHINFVQAFAVEMVITAILMGVILALTDDGNGIPRGPLAP
HHHCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHH
LLIGLLIAVIGASMGPLTGFAMNPARDIGPKAFAWLAGWGDVAFTGGKDIPYFLVPLCAP
HHHHHHHHHHCCCCCCCCCCCCCCHHHCCHHHHHHHHCCCCEEEECCCCCHHHHHHHHHH
VVGAALGAFSYRKLIGRHLPCDTCVEEEQQSPSSSTAQHKASL
HHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: glycerol [Periplasm] [C]

Specific reaction: glycerol [Periplasm] = glycerol [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]