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The map label for this gene is waaA [H]

Identifier: 238897073

GI number: 238897073

Start: 5069753

End: 5071027

Strand: Direct

Name: waaA [H]

Synonym: KP1_5324

Alternate gene names: 238897073

Gene position: 5069753-5071027 (Clockwise)

Preceding gene: 238897072

Following gene: 238897074

Centisome position: 96.59

GC content: 60.63

Gene sequence:

>1275_bases
TTGGAATTGCTTTATACCACCCTACTTTACCTTATTCAGCCGCTGGTGTGGCTGCGGCTGCTGCTGCGCAGTCGTAAGGC
TCCTGCCTATCGCAAACGCTGGGCGGAACGCTATGGCTTCTGTCAGAACAAGGTAGAGCCGGATGGCATTCTGCTGCACT
CCGTTTCCGTCGGCGAGACCTTAGCCGCCATTCCGCTGGTCCGCGCCCTGCGCCACCGCTACCCTTCTCTGCCGATCACC
GTCACCACCATGACGCCGACCGGCTCCGAGCGCGCCATGTCGGCGTTTGGCAAAGATGTTCATCACGTCTATCTGCCCTA
CGATCTGCCCGGCGCCATGAACCGCTTCCTGAACACCGTTCAGCCGAAGCTGGTGATCGTTATGGAGACCGAGCTGTGGC
CGAATATGGTGGCCGCGCTGCATAAGCGTAAGATCCCGCTGGTGATCGCCAACGCGCGCCTGTCCGAACGTTCCGCCAAA
GGCTATGCCAAACTGGGCGGCTTTATGCGCCGCCTGTTGAGCCGCATTACGCTTATCGCCGCGCAGAATGAAGAAGATGG
TAACCGCTTCCTCTCTCTGGGGCTGAAGCGCAACCAGCTGGCGGTCACCGGCAGCCTGAAATTCGATATCTCCGTCACGC
CAGAGCTGGCGGCCCGGGCGGTGACGCTGCGTCGGCAATGGGCACCGCACCGCAAGGTCTGGATTGCCACCAGCACCCAC
GACGGTGAAGAACAAATCATCCTCCAGGCGCATAAAAAACTGCTGGAGACTTTCCCGAACCTGCTGCTGATCCTTGTTCC
CCGCCATCCGGAGCGCTTCCCTGACGCCCGTGAGATGGTACAAAAGGCTGGGATGAGCTTTACTCTGCGCAGCACCGGCG
AGATCCCCTCCTCCAGTACCCAGGTAGTCATTGGCGATACCATGGGCGAGCTGATGCTGCTCTACGGCATTGCCGACCTC
GCCTTCGTCGGCGGCAGCCTGGTGGAGCGCGGCGGCCATAACCCGCTAGAGCCGGCGGCGCACGCCATTCCGGTGCTGAT
GGGCCCGCATACCTTCAACTTCAAAGATATCTGCGCCAAGCTGCAGCAGGACGATGGCCTGATCACCGTTACCGATGCCG
ATTCGCTGGTCAGGGAGGTGTCCACGCTCCTGACCGATGAAGATTACCGTCTGTGGTATGGCCGTCACGCGGTGGAAGTG
CTGCACCAGAACCAGGGCGCGCTGTCGCGTCTGCTACAGTTACTGCAACCTTACCTGCCGCAGCGGAGTCATTAA

Upstream 100 bases:

>100_bases
GCGGGATAGACCAAAAACCCGCTGTTTCCGCCAAAGAGATAGCTTTACGCGGGCTAAATGCTTAGAATTTGCCCGCCAAA
ACAGAGAACGGATAAATAGT

Downstream 100 bases:

>100_bases
TGTCGAACCGGCTTTCCGTGGTGATGATCGCTAAAAACGCCGCCGACCTGCTGCCGGATTGTCTGGGCTCCGTTAGCTGG
GCGGATGAAATTATCGTCCT

Product: 3-deoxy-D-manno-octulosonic-acid transferase

Products: NA

Alternate protein names: KDO transferase [H]

Number of amino acids: Translated: 424; Mature: 424

Protein sequence:

>424_residues
MELLYTTLLYLIQPLVWLRLLLRSRKAPAYRKRWAERYGFCQNKVEPDGILLHSVSVGETLAAIPLVRALRHRYPSLPIT
VTTMTPTGSERAMSAFGKDVHHVYLPYDLPGAMNRFLNTVQPKLVIVMETELWPNMVAALHKRKIPLVIANARLSERSAK
GYAKLGGFMRRLLSRITLIAAQNEEDGNRFLSLGLKRNQLAVTGSLKFDISVTPELAARAVTLRRQWAPHRKVWIATSTH
DGEEQIILQAHKKLLETFPNLLLILVPRHPERFPDAREMVQKAGMSFTLRSTGEIPSSSTQVVIGDTMGELMLLYGIADL
AFVGGSLVERGGHNPLEPAAHAIPVLMGPHTFNFKDICAKLQQDDGLITVTDADSLVREVSTLLTDEDYRLWYGRHAVEV
LHQNQGALSRLLQLLQPYLPQRSH

Sequences:

>Translated_424_residues
MELLYTTLLYLIQPLVWLRLLLRSRKAPAYRKRWAERYGFCQNKVEPDGILLHSVSVGETLAAIPLVRALRHRYPSLPIT
VTTMTPTGSERAMSAFGKDVHHVYLPYDLPGAMNRFLNTVQPKLVIVMETELWPNMVAALHKRKIPLVIANARLSERSAK
GYAKLGGFMRRLLSRITLIAAQNEEDGNRFLSLGLKRNQLAVTGSLKFDISVTPELAARAVTLRRQWAPHRKVWIATSTH
DGEEQIILQAHKKLLETFPNLLLILVPRHPERFPDAREMVQKAGMSFTLRSTGEIPSSSTQVVIGDTMGELMLLYGIADL
AFVGGSLVERGGHNPLEPAAHAIPVLMGPHTFNFKDICAKLQQDDGLITVTDADSLVREVSTLLTDEDYRLWYGRHAVEV
LHQNQGALSRLLQLLQPYLPQRSH
>Mature_424_residues
MELLYTTLLYLIQPLVWLRLLLRSRKAPAYRKRWAERYGFCQNKVEPDGILLHSVSVGETLAAIPLVRALRHRYPSLPIT
VTTMTPTGSERAMSAFGKDVHHVYLPYDLPGAMNRFLNTVQPKLVIVMETELWPNMVAALHKRKIPLVIANARLSERSAK
GYAKLGGFMRRLLSRITLIAAQNEEDGNRFLSLGLKRNQLAVTGSLKFDISVTPELAARAVTLRRQWAPHRKVWIATSTH
DGEEQIILQAHKKLLETFPNLLLILVPRHPERFPDAREMVQKAGMSFTLRSTGEIPSSSTQVVIGDTMGELMLLYGIADL
AFVGGSLVERGGHNPLEPAAHAIPVLMGPHTFNFKDICAKLQQDDGLITVTDADSLVREVSTLLTDEDYRLWYGRHAVEV
LHQNQGALSRLLQLLQPYLPQRSH

Specific function: Essential step in lipopolysaccharides biosynthesis. Acts at transfer of 3-deoxy-D-mono octulonic acid (KDO) from CMP-KDO to a tetraacyldisaccharide 1,4'-bisphosphate precursor of lipid A (lipid IVA). Transfers two molecules of KDO to lipid IVA [H]

COG id: COG1519

COG function: function code M; 3-deoxy-D-manno-octulosonic-acid transferase

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 1 family [H]

Homologues:

Organism=Escherichia coli, GI1790064, Length=424, Percent_Identity=79.9528301886792, Blast_Score=717, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001296
- InterPro:   IPR007507 [H]

Pfam domain/function: PF00534 Glycos_transf_1; PF04413 Glycos_transf_N [H]

EC number: 2.-.-.-

Molecular weight: Translated: 47687; Mature: 47687

Theoretical pI: Translated: 10.03; Mature: 10.03

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MELLYTTLLYLIQPLVWLRLLLRSRKAPAYRKRWAERYGFCQNKVEPDGILLHSVSVGET
CHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCCCCEEEEECCCCHH
LAAIPLVRALRHRYPSLPITVTTMTPTGSERAMSAFGKDVHHVYLPYDLPGAMNRFLNTV
HHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHCCCCEEEEECCCCCHHHHHHHHCC
QPKLVIVMETELWPNMVAALHKRKIPLVIANARLSERSAKGYAKLGGFMRRLLSRITLIA
CCEEEEEEECCCCHHHHHHHHHCCCCEEEECCCCCHHHCCHHHHHHHHHHHHHHHHHEEE
AQNEEDGNRFLSLGLKRNQLAVTGSLKFDISVTPELAARAVTLRRQWAPHRKVWIATSTH
ECCCCCCCCEEEECCCCCEEEEEEEEEEEEEECHHHHHHHHHHHHHCCCCCEEEEEECCC
DGEEQIILQAHKKLLETFPNLLLILVPRHPERFPDAREMVQKAGMSFTLRSTGEIPSSST
CCHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCHHHHHHHCCCEEEEECCCCCCCCCC
QVVIGDTMGELMLLYGIADLAFVGGSLVERGGHNPLEPAAHAIPVLMGPHTFNFKDICAK
EEEECCCHHHHHHHHHHHHHHHHCHHHHHCCCCCCCCHHHHHCCEEECCCCCCHHHHHHH
LQQDDGLITVTDADSLVREVSTLLTDEDYRLWYGRHAVEVLHQNQGALSRLLQLLQPYLP
HHCCCCEEEEECHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHCCCHHHHHHHHHHHHCCC
QRSH
CCCC
>Mature Secondary Structure
MELLYTTLLYLIQPLVWLRLLLRSRKAPAYRKRWAERYGFCQNKVEPDGILLHSVSVGET
CHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCCCCEEEEECCCCHH
LAAIPLVRALRHRYPSLPITVTTMTPTGSERAMSAFGKDVHHVYLPYDLPGAMNRFLNTV
HHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHCCCCEEEEECCCCCHHHHHHHHCC
QPKLVIVMETELWPNMVAALHKRKIPLVIANARLSERSAKGYAKLGGFMRRLLSRITLIA
CCEEEEEEECCCCHHHHHHHHHCCCCEEEECCCCCHHHCCHHHHHHHHHHHHHHHHHEEE
AQNEEDGNRFLSLGLKRNQLAVTGSLKFDISVTPELAARAVTLRRQWAPHRKVWIATSTH
ECCCCCCCCEEEECCCCCEEEEEEEEEEEEEECHHHHHHHHHHHHHCCCCCEEEEEECCC
DGEEQIILQAHKKLLETFPNLLLILVPRHPERFPDAREMVQKAGMSFTLRSTGEIPSSST
CCHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCHHHHHHHCCCEEEEECCCCCCCCCC
QVVIGDTMGELMLLYGIADLAFVGGSLVERGGHNPLEPAAHAIPVLMGPHTFNFKDICAK
EEEECCCHHHHHHHHHHHHHHHHCHHHHHCCCCCCCCHHHHHCCEEECCCCCCHHHHHHH
LQQDDGLITVTDADSLVREVSTLLTDEDYRLWYGRHAVEVLHQNQGALSRLLQLLQPYLP
HHCCCCEEEEECHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHCCCHHHHHHHHHHHHCCC
QRSH
CCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferring phosphorus-containing groups [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]