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The map label for this gene is malS [H]

Identifier: 238897036

GI number: 238897036

Start: 5029156

End: 5031189

Strand: Direct

Name: malS [H]

Synonym: KP1_5283

Alternate gene names: 238897036

Gene position: 5029156-5031189 (Clockwise)

Preceding gene: 238897034

Following gene: 238897037

Centisome position: 95.82

GC content: 59.73

Gene sequence:

>2034_bases
ATGAAACTCGCTGCGCTTGCCACGTTATTCGTGCCTGGGATGGCCTTTGCCGCCTGGACTACCACCGACTTTCCTGCTTT
TACCGAAGAAGGGACCGGCCGATTCATCAGCCAGAAAGTGGTTGAGAAGGGTACACGTCCCCTGCAGCTAAATTTTGACC
AGCAGTGCTGGCAGCCTTCCGGCGGCATTAAGCTCAACCAGATGCTGTCCATGGAGCCCTGTCGCGGCACACCGCCGCAA
TGGCGCATCTTCCGCCAGGGTCTGTATACCCTGGAGGTGGATACCCGTTCCGGCACGCCAACGATGATGATTTCGCTGGA
GGAAAAAGAGACCAGCGCCGCGGCGCCGCAGATCCGCCAGTGTCCGAAGTGGGACGGAAAACCTCTGACCATCGACGTCA
GCAAAACCTTCGCCGAGGGCAGCAAGGTCCGCGATTTCTACAGCGGTAATGTCGCGACCGTCAGCGGCGGCAAAATCACC
CTGCAGCCCGCCTTCGGCAGCAATGGCCTACTGCTGCTTGAGCGCGCCGAGACGGCCGCCCCGGCGCCGTTTGACTGGCA
CAATGCCACCGTCTACTTCGTCCTGACCGATCGCTTCGTAAACGGTAACCCGGCCAACGACAACAGCTATGGTCGCCATA
AAGACGGCATGCAGGAGATCGGCACCTTCCACGGCGGCGACCTGCAGGGATTAACCAGCAAGCTGGATTATCTGCAGCAG
ATGGGAGTTAACGCCCTGTGGATCAGCTCCCCGCTCGAGCAGATCCACGGCTGGGTCGGCGGCGGCACGAAGGGCGATTT
CCCTCACTACGCTTATCACGGCTATTACACCCAGGACTGGTCGAAACTGGACGCCAACATGGGGACCGAAGCGGATCTCC
GCCGACTGGTGGATGAAGCGCATAAGCGCGGAATACGTATTCTGTTCGACGTGGTGATGAACCATGCCGGCTACGCCACC
CTGGCCGACATGCAGGAGTTCCAGTTCGGCTCCCTGTATCTGCAAGGAGATGAGCTGAAAAAAACTCTCGGCGAGCGCTG
GACGGACTGGAAGCCGGGCGCGGGACAGACCTGGCACAGCTTTAACGACTATATCAACTTCAGCGACAAAGCCGGGTGGG
AGAAATGGTGGGGTAAGAAGTGGATCCGCACCGATATCGGCGATTACGACAATCCGGGCTATGACGATCTCACCATGTCG
CTGGCCTTCCTGCCGGACCTGAAAACCGAATCGAAGGAGGTCTCAGGCCTGCCGAACTTCTATAACCATAAACCAGACAC
CGCAGCGAAAGCGATCCCGGGCTATACGCCGCGCGACTATCTGACACACTGGCTGAGCCAGTGGGTACGTGACTACGGCA
TCGATGGCTTCCGCGTCGATACCGCCAAACATGTGGAGATGGACGCCTGGCAGCAGTTAAAAACCCAGGCCACCGCCGCG
CTGGCGGAGTGGAAGAAAGCCAATCCGGATAAAGCGCTGGATGCGGCGCCGTTCTGGATGACCGGCGAAGCGTGGGGACA
TGGAGTGATGCAGAGCGATTATTATCGCCACGGTTTTGATGCGATGATCAATTTCGACTACCAGGACCAGGCGGCGAAAG
CGGCGACCTGTATGGCCAATATTGACCTCACCTGGCAGCAAATGGCGGACAAGCTGCAGAGCTTTAACGTCCTGAGCTAC
CTCTCCTCGCACGATACGCGGCTGTTCCGCGAAGGCGGCACCACCGCGGCGGAGCTACTGCTGCTGGCCCCTGGCGCCGT
GCAGATTTTCTATGGCGACGAATCATCGCGCCCGTTCGGCCCCACCGGCTCGGACCCGCTGCAGGGTACCCGCTCGGAGA
TGAACTGGCAGGATGTCAACGGCAAGGCGGCCCGCAGCGTTACCCACTGGCAGAAGATCGGCCAGTTCCGCGCTCGCCAT
CCGGCAATCGGCATGGGCAAACAGACAACGCTGTCGATGCCACGTGGTTACGGGTTCGTGCGTGAGAGCGGTGAAGACAA
AGTGATGGTCATCTGGGCCGGGCAGCAGCAGTAA

Upstream 100 bases:

>100_bases
ACTCACCCCTTTTCATCCTCCTGGTAAAACGCGCGTCAGGAGGAGTTCACCCGCGCGGCGACAAGGCACACTAGATAAAA
ACCGCTCACAGGATCTCGCA

Downstream 100 bases:

>100_bases
TCCACATGTGGCTCTCCGCCCTGCGGGGAGCCACCATTCAGAACAAAATGCTAACAAAATAGCAATATACAGGATAAAGA
TAAAAACCTGCCTCCTCCCG

Product: periplasmic alpha-amylase precursor

Products: NA

Alternate protein names: 1,4-alpha-D-glucan glucanohydrolase [H]

Number of amino acids: Translated: 677; Mature: 677

Protein sequence:

>677_residues
MKLAALATLFVPGMAFAAWTTTDFPAFTEEGTGRFISQKVVEKGTRPLQLNFDQQCWQPSGGIKLNQMLSMEPCRGTPPQ
WRIFRQGLYTLEVDTRSGTPTMMISLEEKETSAAAPQIRQCPKWDGKPLTIDVSKTFAEGSKVRDFYSGNVATVSGGKIT
LQPAFGSNGLLLLERAETAAPAPFDWHNATVYFVLTDRFVNGNPANDNSYGRHKDGMQEIGTFHGGDLQGLTSKLDYLQQ
MGVNALWISSPLEQIHGWVGGGTKGDFPHYAYHGYYTQDWSKLDANMGTEADLRRLVDEAHKRGIRILFDVVMNHAGYAT
LADMQEFQFGSLYLQGDELKKTLGERWTDWKPGAGQTWHSFNDYINFSDKAGWEKWWGKKWIRTDIGDYDNPGYDDLTMS
LAFLPDLKTESKEVSGLPNFYNHKPDTAAKAIPGYTPRDYLTHWLSQWVRDYGIDGFRVDTAKHVEMDAWQQLKTQATAA
LAEWKKANPDKALDAAPFWMTGEAWGHGVMQSDYYRHGFDAMINFDYQDQAAKAATCMANIDLTWQQMADKLQSFNVLSY
LSSHDTRLFREGGTTAAELLLLAPGAVQIFYGDESSRPFGPTGSDPLQGTRSEMNWQDVNGKAARSVTHWQKIGQFRARH
PAIGMGKQTTLSMPRGYGFVRESGEDKVMVIWAGQQQ

Sequences:

>Translated_677_residues
MKLAALATLFVPGMAFAAWTTTDFPAFTEEGTGRFISQKVVEKGTRPLQLNFDQQCWQPSGGIKLNQMLSMEPCRGTPPQ
WRIFRQGLYTLEVDTRSGTPTMMISLEEKETSAAAPQIRQCPKWDGKPLTIDVSKTFAEGSKVRDFYSGNVATVSGGKIT
LQPAFGSNGLLLLERAETAAPAPFDWHNATVYFVLTDRFVNGNPANDNSYGRHKDGMQEIGTFHGGDLQGLTSKLDYLQQ
MGVNALWISSPLEQIHGWVGGGTKGDFPHYAYHGYYTQDWSKLDANMGTEADLRRLVDEAHKRGIRILFDVVMNHAGYAT
LADMQEFQFGSLYLQGDELKKTLGERWTDWKPGAGQTWHSFNDYINFSDKAGWEKWWGKKWIRTDIGDYDNPGYDDLTMS
LAFLPDLKTESKEVSGLPNFYNHKPDTAAKAIPGYTPRDYLTHWLSQWVRDYGIDGFRVDTAKHVEMDAWQQLKTQATAA
LAEWKKANPDKALDAAPFWMTGEAWGHGVMQSDYYRHGFDAMINFDYQDQAAKAATCMANIDLTWQQMADKLQSFNVLSY
LSSHDTRLFREGGTTAAELLLLAPGAVQIFYGDESSRPFGPTGSDPLQGTRSEMNWQDVNGKAARSVTHWQKIGQFRARH
PAIGMGKQTTLSMPRGYGFVRESGEDKVMVIWAGQQQ
>Mature_677_residues
MKLAALATLFVPGMAFAAWTTTDFPAFTEEGTGRFISQKVVEKGTRPLQLNFDQQCWQPSGGIKLNQMLSMEPCRGTPPQ
WRIFRQGLYTLEVDTRSGTPTMMISLEEKETSAAAPQIRQCPKWDGKPLTIDVSKTFAEGSKVRDFYSGNVATVSGGKIT
LQPAFGSNGLLLLERAETAAPAPFDWHNATVYFVLTDRFVNGNPANDNSYGRHKDGMQEIGTFHGGDLQGLTSKLDYLQQ
MGVNALWISSPLEQIHGWVGGGTKGDFPHYAYHGYYTQDWSKLDANMGTEADLRRLVDEAHKRGIRILFDVVMNHAGYAT
LADMQEFQFGSLYLQGDELKKTLGERWTDWKPGAGQTWHSFNDYINFSDKAGWEKWWGKKWIRTDIGDYDNPGYDDLTMS
LAFLPDLKTESKEVSGLPNFYNHKPDTAAKAIPGYTPRDYLTHWLSQWVRDYGIDGFRVDTAKHVEMDAWQQLKTQATAA
LAEWKKANPDKALDAAPFWMTGEAWGHGVMQSDYYRHGFDAMINFDYQDQAAKAATCMANIDLTWQQMADKLQSFNVLSY
LSSHDTRLFREGGTTAAELLLLAPGAVQIFYGDESSRPFGPTGSDPLQGTRSEMNWQDVNGKAARSVTHWQKIGQFRARH
PAIGMGKQTTLSMPRGYGFVRESGEDKVMVIWAGQQQ

Specific function: Since only maltooligosaccharides up to a chain length of 6 glucose units are actively transported through the cytoplasmic membrane via the membrane-bound complex of three proteins, MalF, MalG, and MalK, longer maltooligosaccharides must first be degraded

COG id: COG0366

COG function: function code G; Glycosidases

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 13 family [H]

Homologues:

Organism=Escherichia coli, GI1789995, Length=677, Percent_Identity=79.4682422451994, Blast_Score=1144, Evalue=0.0,
Organism=Escherichia coli, GI1786604, Length=524, Percent_Identity=24.4274809160305, Blast_Score=79, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6322245, Length=136, Percent_Identity=31.6176470588235, Blast_Score=69, Evalue=3e-12,
Organism=Drosophila melanogaster, GI221330053, Length=147, Percent_Identity=34.6938775510204, Blast_Score=67, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014635
- InterPro:   IPR006047
- InterPro:   IPR006589
- InterPro:   IPR017853
- InterPro:   IPR013781 [H]

Pfam domain/function: PF00128 Alpha-amylase [H]

EC number: =3.2.1.1 [H]

Molecular weight: Translated: 75964; Mature: 75964

Theoretical pI: Translated: 6.28; Mature: 6.28

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLAALATLFVPGMAFAAWTTTDFPAFTEEGTGRFISQKVVEKGTRPLQLNFDQQCWQPS
CCHHHHHHHHHCCHHHEEECCCCCCCCCCCCCCHHHHHHHHHCCCCEEEECCCHHHCCCC
GGIKLNQMLSMEPCRGTPPQWRIFRQGLYTLEVDTRSGTPTMMISLEEKETSAAAPQIRQ
CCEEEHHHCCCCCCCCCCHHHHHHHCCCEEEEEECCCCCCEEEEEEECCCCCCCCHHHHC
CPKWDGKPLTIDVSKTFAEGSKVRDFYSGNVATVSGGKITLQPAFGSNGLLLLERAETAA
CCCCCCCEEEEECCHHHHCCCHHHHHHCCCEEEECCCEEEEEECCCCCCEEEEEECCCCC
PAPFDWHNATVYFVLTDRFVNGNPANDNSYGRHKDGMQEIGTFHGGDLQGLTSKLDYLQQ
CCCCCCCCCEEEEEEEECCCCCCCCCCCCCCCCCCCHHHHHCCCCCCHHHHHHHHHHHHH
MGVNALWISSPLEQIHGWVGGGTKGDFPHYAYHGYYTQDWSKLDANMGTEADLRRLVDEA
CCCCEEEECCHHHHHCCCCCCCCCCCCCCEEECCEECCCHHHHHCCCCCHHHHHHHHHHH
HKRGIRILFDVVMNHAGYATLADMQEFQFGSLYLQGDELKKTLGERWTDWKPGAGQTWHS
HHCCHHHHHHHHHCCCCCEEHHHHHHCCCCEEEEECHHHHHHHHCCCCCCCCCCCCCCCC
FNDYINFSDKAGWEKWWGKKWIRTDIGDYDNPGYDDLTMSLAFLPDLKTESKEVSGLPNF
HHHCCCCCCCCCHHHHHCCCHHHHCCCCCCCCCCCHHEEEEEECCCCCCCCHHHCCCCCH
YNHKPDTAAKAIPGYTPRDYLTHWLSQWVRDYGIDGFRVDTAKHVEMDAWQQLKTQATAA
HCCCCCHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCEEECCHHHCCHHHHHHHHHHHHHH
LAEWKKANPDKALDAAPFWMTGEAWGHGVMQSDYYRHGFDAMINFDYQDQAAKAATCMAN
HHHHHCCCCCCCCCCCCCEEECHHHCCCCCCCCHHHCCCCEEEECCCHHHHHHHHHHHEE
IDLTWQQMADKLQSFNVLSYLSSHDTRLFREGGTTAAELLLLAPGAVQIFYGDESSRPFG
CCCCHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCHHHHHEEECCCEEEEEECCCCCCCCC
PTGSDPLQGTRSEMNWQDVNGKAARSVTHWQKIGQFRARHPAIGMGKQTTLSMPRGYGFV
CCCCCCCCCCHHCCCEECCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCEECCCCCCCHH
RESGEDKVMVIWAGQQQ
CCCCCCEEEEEECCCCC
>Mature Secondary Structure
MKLAALATLFVPGMAFAAWTTTDFPAFTEEGTGRFISQKVVEKGTRPLQLNFDQQCWQPS
CCHHHHHHHHHCCHHHEEECCCCCCCCCCCCCCHHHHHHHHHCCCCEEEECCCHHHCCCC
GGIKLNQMLSMEPCRGTPPQWRIFRQGLYTLEVDTRSGTPTMMISLEEKETSAAAPQIRQ
CCEEEHHHCCCCCCCCCCHHHHHHHCCCEEEEEECCCCCCEEEEEEECCCCCCCCHHHHC
CPKWDGKPLTIDVSKTFAEGSKVRDFYSGNVATVSGGKITLQPAFGSNGLLLLERAETAA
CCCCCCCEEEEECCHHHHCCCHHHHHHCCCEEEECCCEEEEEECCCCCCEEEEEECCCCC
PAPFDWHNATVYFVLTDRFVNGNPANDNSYGRHKDGMQEIGTFHGGDLQGLTSKLDYLQQ
CCCCCCCCCEEEEEEEECCCCCCCCCCCCCCCCCCCHHHHHCCCCCCHHHHHHHHHHHHH
MGVNALWISSPLEQIHGWVGGGTKGDFPHYAYHGYYTQDWSKLDANMGTEADLRRLVDEA
CCCCEEEECCHHHHHCCCCCCCCCCCCCCEEECCEECCCHHHHHCCCCCHHHHHHHHHHH
HKRGIRILFDVVMNHAGYATLADMQEFQFGSLYLQGDELKKTLGERWTDWKPGAGQTWHS
HHCCHHHHHHHHHCCCCCEEHHHHHHCCCCEEEEECHHHHHHHHCCCCCCCCCCCCCCCC
FNDYINFSDKAGWEKWWGKKWIRTDIGDYDNPGYDDLTMSLAFLPDLKTESKEVSGLPNF
HHHCCCCCCCCCHHHHHCCCHHHHCCCCCCCCCCCHHEEEEEECCCCCCCCHHHCCCCCH
YNHKPDTAAKAIPGYTPRDYLTHWLSQWVRDYGIDGFRVDTAKHVEMDAWQQLKTQATAA
HCCCCCHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCEEECCHHHCCHHHHHHHHHHHHHH
LAEWKKANPDKALDAAPFWMTGEAWGHGVMQSDYYRHGFDAMINFDYQDQAAKAATCMAN
HHHHHCCCCCCCCCCCCCEEECHHHCCCCCCCCHHHCCCCEEEECCCHHHHHHHHHHHEE
IDLTWQQMADKLQSFNVLSYLSSHDTRLFREGGTTAAELLLLAPGAVQIFYGDESSRPFG
CCCCHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCHHHHHEEECCCEEEEEECCCCCCCCC
PTGSDPLQGTRSEMNWQDVNGKAARSVTHWQKIGQFRARHPAIGMGKQTTLSMPRGYGFV
CCCCCCCCCCHHCCCEECCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCEECCCCCCCHH
RESGEDKVMVIWAGQQQ
CCCCCCEEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1544897; 8041620; 9278503; 9268356 [H]