Click here to switch to the map view.

The map label for this gene is treF [H]

Identifier: 238896967

GI number: 238896967

Start: 4942892

End: 4944544

Strand: Direct

Name: treF [H]

Synonym: KP1_5209

Alternate gene names: 238896967

Gene position: 4942892-4944544 (Clockwise)

Preceding gene: 238896966

Following gene: 238896968

Centisome position: 94.18

GC content: 60.98

Gene sequence:

>1653_bases
ATGTTCAGCCAGAAATTACGCCACGTTGAAGACGACGAGTTAAGGATCGACATCGATCCCTGCTACGAAGCCGATCCGTA
CGAATTAAAGCTGGATGAGATGATAGACGCTGAACCGGAGCCTGAGGTGATCGAGGGGCTTCCGGCGTCCGATGCCCTCA
CCCCGGCGGATCGCTATCTTGAGCTGTTTACCAACGTGCAGAAATCACGCATTTTCGCGGACAGCAAAACCTTCCCCGAC
TGCGCGCCGAAACACGATCCGCTGGACATCCTGCGGAACTACCGCAAGGTCAAACGCCAGCCCGACTTCGATCTGCGGCA
GTTCGTTGAGGACAACTTCTGGCTGCCGGAGAGCCAGAGCGACATCTATATCTCCGACCCCAGCCTGACCCTGAAAGAAC
ACATCGACAAGCTGTGGCCGGTGCTGACCCGCGAGCCTCAGGATCATATCCCGTGGTCATCGCTACTGGCGCTGCCGCAG
GCCTACATCGTGCCCGGCGGTCGCTTTAGCGAAACCTATTACTGGGACTCTTATTTCACCATGCTGGGGCTGGCGGAGAG
CGGCCGGGAAGATTTGCTGAAATGCATGGCCGATAACTTTGCCTGGCTGATTGAAACCTACGGCCACATTCCGAACGGCA
ACCGCACCTACTATCTCAGCCGCTCGCAGCCGCCGGTGTTCGCCCTGATGGTCGAACTCTTTGAAGAGGATGGCGTGCGC
GGCGCGAAGCGCTATCTGGACCATCTGAAGATGGAGCACGCCTTCTGGATGGACGGCGCCGAGTCGTTGATCCCGCACCA
GGCGTATCGCCACGTGGTGCGTATGCCGGATGGCTCCCTGCTCAATCGCTACTGGGACGACCGTGACACGCCGCGGGATG
AGTCGTGGCGCGAAGACGTGGAGACCGCCCGACACTCCGGGCGGCCCGCCAATGAAGTATACCGCGACCTGCGGGCGGGC
GCCGCGTCCGGCTGGGACTACTCCAGCCGCTGGCTGCGGGATATCACGCGGCTGGCCAGCATCCGCACTACGCAGTTTAT
CCCCATCGACCTGAACGCCTTCCTGTTCAAGCTGGAGACGACCATTGCCAACCTCTCCGGCCTGAAGGGCGATCGCGAGA
CGGAGGCGGCCTTCCGCCAGAAGGCCCAGGATCGGCGGGCGGCGGTCAACCGCTATCTGTGGGATGATGAGAACGGCTGT
TTCCGCGATTATGACTGGCGCCGTGAACAGCTGGCGCTGTTTTCCGCAGCCAGTATCGTCACCCTTTACGTCGGCCTGGC
GACCCACGAACAGGCAGAACGCCTTGCCGATGCGGTGCGGGCCCGCCTGCTCACCCCCGGGGGGATCATGGCCACCGAAT
ACGAAAGCGGTGAGCAGTGGGATAAACCCAACGGCTGGGCGCCGCTGCAGTGGATGGCGATCCAGGGATTTAAACGTTAC
GGCCAGGATCCGCTGGGAGATGAGATTGCCTGGAGCTGGCTGCAGACGGTCAACCACTTTTACAAGCAGCATCACAAGCT
GATTGAAAAATACCATATTGCCACCGGCGTCCCCCATGAGGGCGGCGGCGGGGAGTATCCCCTGCAGGATGGCTTTGGCT
GGACCAACGGCGTGGTGCGCCGACTGATTGGCCTGTACGGTGAGCCAACCTAG

Upstream 100 bases:

>100_bases
ACAGTTTGCTTCACACTCACCGAAGCGACTGTCTACGCTTAATAGACAAGGCCGGGTATGCCCGGCCCCGAACTCACGCT
ATCCGGAGGTCCGTATCGCT

Downstream 100 bases:

>100_bases
TCGGGCTGTTCCCGGCGGCTTTCTCCTGCTATGTTCAGAGGATTCGCCCTGCCCGGAACGACGCTTATGCCTGAACTTCT
GACGCCGCGCCTGCGCTGCT

Product: trehalase

Products: NA

Alternate protein names: Alpha,alpha-trehalase; Alpha,alpha-trehalose glucohydrolase [H]

Number of amino acids: Translated: 550; Mature: 550

Protein sequence:

>550_residues
MFSQKLRHVEDDELRIDIDPCYEADPYELKLDEMIDAEPEPEVIEGLPASDALTPADRYLELFTNVQKSRIFADSKTFPD
CAPKHDPLDILRNYRKVKRQPDFDLRQFVEDNFWLPESQSDIYISDPSLTLKEHIDKLWPVLTREPQDHIPWSSLLALPQ
AYIVPGGRFSETYYWDSYFTMLGLAESGREDLLKCMADNFAWLIETYGHIPNGNRTYYLSRSQPPVFALMVELFEEDGVR
GAKRYLDHLKMEHAFWMDGAESLIPHQAYRHVVRMPDGSLLNRYWDDRDTPRDESWREDVETARHSGRPANEVYRDLRAG
AASGWDYSSRWLRDITRLASIRTTQFIPIDLNAFLFKLETTIANLSGLKGDRETEAAFRQKAQDRRAAVNRYLWDDENGC
FRDYDWRREQLALFSAASIVTLYVGLATHEQAERLADAVRARLLTPGGIMATEYESGEQWDKPNGWAPLQWMAIQGFKRY
GQDPLGDEIAWSWLQTVNHFYKQHHKLIEKYHIATGVPHEGGGGEYPLQDGFGWTNGVVRRLIGLYGEPT

Sequences:

>Translated_550_residues
MFSQKLRHVEDDELRIDIDPCYEADPYELKLDEMIDAEPEPEVIEGLPASDALTPADRYLELFTNVQKSRIFADSKTFPD
CAPKHDPLDILRNYRKVKRQPDFDLRQFVEDNFWLPESQSDIYISDPSLTLKEHIDKLWPVLTREPQDHIPWSSLLALPQ
AYIVPGGRFSETYYWDSYFTMLGLAESGREDLLKCMADNFAWLIETYGHIPNGNRTYYLSRSQPPVFALMVELFEEDGVR
GAKRYLDHLKMEHAFWMDGAESLIPHQAYRHVVRMPDGSLLNRYWDDRDTPRDESWREDVETARHSGRPANEVYRDLRAG
AASGWDYSSRWLRDITRLASIRTTQFIPIDLNAFLFKLETTIANLSGLKGDRETEAAFRQKAQDRRAAVNRYLWDDENGC
FRDYDWRREQLALFSAASIVTLYVGLATHEQAERLADAVRARLLTPGGIMATEYESGEQWDKPNGWAPLQWMAIQGFKRY
GQDPLGDEIAWSWLQTVNHFYKQHHKLIEKYHIATGVPHEGGGGEYPLQDGFGWTNGVVRRLIGLYGEPT
>Mature_550_residues
MFSQKLRHVEDDELRIDIDPCYEADPYELKLDEMIDAEPEPEVIEGLPASDALTPADRYLELFTNVQKSRIFADSKTFPD
CAPKHDPLDILRNYRKVKRQPDFDLRQFVEDNFWLPESQSDIYISDPSLTLKEHIDKLWPVLTREPQDHIPWSSLLALPQ
AYIVPGGRFSETYYWDSYFTMLGLAESGREDLLKCMADNFAWLIETYGHIPNGNRTYYLSRSQPPVFALMVELFEEDGVR
GAKRYLDHLKMEHAFWMDGAESLIPHQAYRHVVRMPDGSLLNRYWDDRDTPRDESWREDVETARHSGRPANEVYRDLRAG
AASGWDYSSRWLRDITRLASIRTTQFIPIDLNAFLFKLETTIANLSGLKGDRETEAAFRQKAQDRRAAVNRYLWDDENGC
FRDYDWRREQLALFSAASIVTLYVGLATHEQAERLADAVRARLLTPGGIMATEYESGEQWDKPNGWAPLQWMAIQGFKRY
GQDPLGDEIAWSWLQTVNHFYKQHHKLIEKYHIATGVPHEGGGGEYPLQDGFGWTNGVVRRLIGLYGEPT

Specific function: Hydrolyzes trehalose to glucose. Could be involved, in cells returning to low osmolarity conditions, in the utilization of the accumulated cytoplasmic trehalose, which was synthesized in response to high osmolarity [H]

COG id: COG1626

COG function: function code G; Neutral trehalase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 37 family [H]

Homologues:

Organism=Homo sapiens, GI116284412, Length=526, Percent_Identity=34.2205323193916, Blast_Score=249, Evalue=5e-66,
Organism=Escherichia coli, GI1789936, Length=549, Percent_Identity=84.8816029143898, Blast_Score=974, Evalue=0.0,
Organism=Escherichia coli, GI1787447, Length=487, Percent_Identity=51.3347022587269, Blast_Score=509, Evalue=1e-145,
Organism=Caenorhabditis elegans, GI17542196, Length=525, Percent_Identity=32.7619047619048, Blast_Score=256, Evalue=3e-68,
Organism=Caenorhabditis elegans, GI17565078, Length=519, Percent_Identity=31.4065510597302, Blast_Score=230, Evalue=2e-60,
Organism=Caenorhabditis elegans, GI25148109, Length=547, Percent_Identity=29.0676416819013, Blast_Score=224, Evalue=1e-58,
Organism=Caenorhabditis elegans, GI25141398, Length=541, Percent_Identity=31.2384473197782, Blast_Score=222, Evalue=5e-58,
Organism=Caenorhabditis elegans, GI71987755, Length=425, Percent_Identity=30.8235294117647, Blast_Score=187, Evalue=1e-47,
Organism=Saccharomyces cerevisiae, GI6320204, Length=435, Percent_Identity=29.8850574712644, Blast_Score=160, Evalue=6e-40,
Organism=Saccharomyces cerevisiae, GI6319473, Length=444, Percent_Identity=30.1801801801802, Blast_Score=149, Evalue=1e-36,
Organism=Drosophila melanogaster, GI24656680, Length=548, Percent_Identity=31.2043795620438, Blast_Score=229, Evalue=5e-60,
Organism=Drosophila melanogaster, GI24656675, Length=548, Percent_Identity=31.2043795620438, Blast_Score=229, Evalue=5e-60,
Organism=Drosophila melanogaster, GI24656661, Length=523, Percent_Identity=31.5487571701721, Blast_Score=228, Evalue=9e-60,
Organism=Drosophila melanogaster, GI17933716, Length=523, Percent_Identity=31.5487571701721, Blast_Score=228, Evalue=9e-60,
Organism=Drosophila melanogaster, GI24656670, Length=523, Percent_Identity=31.5487571701721, Blast_Score=228, Evalue=9e-60,
Organism=Drosophila melanogaster, GI24656685, Length=484, Percent_Identity=31.8181818181818, Blast_Score=215, Evalue=6e-56,
Organism=Drosophila melanogaster, GI22024178, Length=535, Percent_Identity=26.3551401869159, Blast_Score=173, Evalue=2e-43,
Organism=Drosophila melanogaster, GI45551104, Length=376, Percent_Identity=26.3297872340426, Blast_Score=129, Evalue=6e-30,
Organism=Drosophila melanogaster, GI28573474, Length=298, Percent_Identity=27.1812080536913, Blast_Score=101, Evalue=1e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008928
- InterPro:   IPR001661
- InterPro:   IPR018232 [H]

Pfam domain/function: PF01204 Trehalase [H]

EC number: =3.2.1.28 [H]

Molecular weight: Translated: 63757; Mature: 63757

Theoretical pI: Translated: 4.88; Mature: 4.88

Prosite motif: PS00927 TREHALASE_1 ; PS00928 TREHALASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFSQKLRHVEDDELRIDIDPCYEADPYELKLDEMIDAEPEPEVIEGLPASDALTPADRYL
CCCHHHCCCCCCCEEEEECCCCCCCCCEEEHHHHHCCCCCHHHHCCCCCCCCCCHHHHHH
ELFTNVQKSRIFADSKTFPDCAPKHDPLDILRNYRKVKRQPDFDLRQFVEDNFWLPESQS
HHHHHHHHHHEEECCCCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCC
DIYISDPSLTLKEHIDKLWPVLTREPQDHIPWSSLLALPQAYIVPGGRFSETYYWDSYFT
CEEEECCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHCCHHEECCCCCCCCCEEEHHHHH
MLGLAESGREDLLKCMADNFAWLIETYGHIPNGNRTYYLSRSQPPVFALMVELFEEDGVR
HHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCHHHHHHHHHHHCCCH
GAKRYLDHLKMEHAFWMDGAESLIPHQAYRHVVRMPDGSLLNRYWDDRDTPRDESWREDV
HHHHHHHHHHHHHHHHCCCHHHHCCHHHHHHHHCCCCCHHHHHHCCCCCCCCCHHHHHHH
ETARHSGRPANEVYRDLRAGAASGWDYSSRWLRDITRLASIRTTQFIPIDLNAFLFKLET
HHHHHCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCEEEEEEHHHHHHHHHH
TIANLSGLKGDRETEAAFRQKAQDRRAAVNRYLWDDENGCFRDYDWRREQLALFSAASIV
HHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHH
TLYVGLATHEQAERLADAVRARLLTPGGIMATEYESGEQWDKPNGWAPLQWMAIQGFKRY
HHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHH
GQDPLGDEIAWSWLQTVNHFYKQHHKLIEKYHIATGVPHEGGGGEYPLQDGFGWTNGVVR
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHH
RLIGLYGEPT
HHHHHCCCCC
>Mature Secondary Structure
MFSQKLRHVEDDELRIDIDPCYEADPYELKLDEMIDAEPEPEVIEGLPASDALTPADRYL
CCCHHHCCCCCCCEEEEECCCCCCCCCEEEHHHHHCCCCCHHHHCCCCCCCCCCHHHHHH
ELFTNVQKSRIFADSKTFPDCAPKHDPLDILRNYRKVKRQPDFDLRQFVEDNFWLPESQS
HHHHHHHHHHEEECCCCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCC
DIYISDPSLTLKEHIDKLWPVLTREPQDHIPWSSLLALPQAYIVPGGRFSETYYWDSYFT
CEEEECCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHCCHHEECCCCCCCCCEEEHHHHH
MLGLAESGREDLLKCMADNFAWLIETYGHIPNGNRTYYLSRSQPPVFALMVELFEEDGVR
HHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCHHHHHHHHHHHCCCH
GAKRYLDHLKMEHAFWMDGAESLIPHQAYRHVVRMPDGSLLNRYWDDRDTPRDESWREDV
HHHHHHHHHHHHHHHHCCCHHHHCCHHHHHHHHCCCCCHHHHHHCCCCCCCCCHHHHHHH
ETARHSGRPANEVYRDLRAGAASGWDYSSRWLRDITRLASIRTTQFIPIDLNAFLFKLET
HHHHHCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCEEEEEEHHHHHHHHHH
TIANLSGLKGDRETEAAFRQKAQDRRAAVNRYLWDDENGCFRDYDWRREQLALFSAASIV
HHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHH
TLYVGLATHEQAERLADAVRARLLTPGGIMATEYESGEQWDKPNGWAPLQWMAIQGFKRY
HHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHH
GQDPLGDEIAWSWLQTVNHFYKQHHKLIEKYHIATGVPHEGGGGEYPLQDGFGWTNGVVR
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHH
RLIGLYGEPT
HHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA