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The map label for this gene is pitA [H]

Identifier: 238896956

GI number: 238896956

Start: 4929419

End: 4930915

Strand: Direct

Name: pitA [H]

Synonym: KP1_5198

Alternate gene names: 238896956

Gene position: 4929419-4930915 (Clockwise)

Preceding gene: 238896954

Following gene: 238896958

Centisome position: 93.92

GC content: 56.25

Gene sequence:

>1497_bases
ATGCTACATTTATTTGCCGGTCTGGATTTACATACCGGACTATTATTATTGCTTGCTTTAGCTTTTGTACTGTTTTACGA
AGCGATTAACGGTTTCCATGATACCGCAAACGCGGTGGCTACGGTTATCTACACTCGCGCAATGCGCTCACAGCTCGCTG
TGGCGATGGCGGCGCTCTTCAACTTCTTCGGCGTTCTGCTGGGCGGTCTCAGCGTAGCCTATGCCATCGTGCATATGCTG
CCGACCGATCTGCTGCTTAACATGGGTTCCGCACATGGCCTCGCCATGGTGTTCTCTATGCTGCTGGCGGCGATCATCTG
GAACCTCGGCACCTGGTACTTCGGTCTTCCAGCTTCCAGTTCTCATACCCTGATTGGCGCCATCATCGGCATTGGTTTAA
CCAATGCGCTGATGACCGGTACTTCCGTGGTTGATGCGCTGAATATCCCGAAAGTCATCGGTATTTTTGCTTCGCTTATC
ATCTCGCCGATCGTCGGTCTGGTGATCGCGGGCGGCCTGATTTTCCTGCTGCGTCGCTACTGGAGCGGAACCAAAAAACG
CGCGCGTATCCACCTAACGCCCGCAGAGCGTGAAAAGAAAGACGGTAAGAAAAAGCCGCCGTTCTGGACCCGTATCGCGC
TGATCCTGTCTGCTATTGGCGTGTCGTTCTCTCACGGTGCTAACGATGGGCAGAAAGGCATTGGCCTGGTGATGCTGGTG
CTGATCGGCGTCGCGCCGGCGGGTTTCGTGGTGAATATGAATGCCTCTGGTTACGAAATCACCCGTACCCGCGATGCGGT
CAACAACGTGGAAACCTTCTTCCAGCAGCGTCCTGAACTGCTGAAAAAAGCCACCGGCGTCGATCAGCTGGTGCCTTCTC
CGGATACCAATACCGCGGCCAATGGCGAGTTCCACTGCCATCCGGCGAACACCATCAACGCCCTTGACCGCGTCAAAACC
ATGCTGACCGGCGTTGAGAACTACGAGTCGCTAAAACCGGAACAGCGCGGCCAGCTGCGTCGCATCATGCTCTGCATCTC
CGACACCACCGACAAGGTGGCGAAGCTGCCGGATGTGAGCGCTGACGACCAGCGTCTGCTGAAGAAACTGAAAACCGATA
TGCTGAGCACCATCGAGTACGCGCCAATCTGGATTATCATGGCTGTCGCGCTGGCGCTGGGTATCGGCACCATGATCGGC
TGGCGCCGCGTGGCGACCACCATCGGTGAGAAGATTGGTAAGAAAGGCATGACCTACGCTCAGGGGATGTCGGCTCAGAT
GACCGCAGCGGTTTCTATCGGTCTGGCGAGCTACACCGGGATGCCGGTTTCCACCACCCACGTACTCTCCTCCTCGGTAG
CGGGGACGATGCTGGTGGATGGCGGTGGTCTGCAGAAAAAGACGGTGACCAGCATTCTGATGGCCTGGGTGCTGACCCTG
CCGGCAGCGATTATCCTTTCAGGCGTCCTTTACTGGCTCTCCCTGAAACTGATTTAA

Upstream 100 bases:

>100_bases
CAGGAAGAAAATCAAAAAAAGTCTATATTTCACTTTGCCCGCGCCGCTAAAGTCACTGATAATGCGCCGCGTTCATGTCC
TCAAAATGGCGTAACGTCCT

Downstream 100 bases:

>100_bases
GCGTTATTCGTTCAAAACGGGCTAGCTTGCTGGCCCGTTTTTTTATTCTGAAACGTTAGTGCCAGATCATCAGCGCAATC
AGGCTCACCACCACCAGGCC

Product: putative low-affinity inorganic phosphate transporter

Products: Proton [Cytoplasm]; phosphate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 498; Mature: 498

Protein sequence:

>498_residues
MLHLFAGLDLHTGLLLLLALAFVLFYEAINGFHDTANAVATVIYTRAMRSQLAVAMAALFNFFGVLLGGLSVAYAIVHML
PTDLLLNMGSAHGLAMVFSMLLAAIIWNLGTWYFGLPASSSHTLIGAIIGIGLTNALMTGTSVVDALNIPKVIGIFASLI
ISPIVGLVIAGGLIFLLRRYWSGTKKRARIHLTPAEREKKDGKKKPPFWTRIALILSAIGVSFSHGANDGQKGIGLVMLV
LIGVAPAGFVVNMNASGYEITRTRDAVNNVETFFQQRPELLKKATGVDQLVPSPDTNTAANGEFHCHPANTINALDRVKT
MLTGVENYESLKPEQRGQLRRIMLCISDTTDKVAKLPDVSADDQRLLKKLKTDMLSTIEYAPIWIIMAVALALGIGTMIG
WRRVATTIGEKIGKKGMTYAQGMSAQMTAAVSIGLASYTGMPVSTTHVLSSSVAGTMLVDGGGLQKKTVTSILMAWVLTL
PAAIILSGVLYWLSLKLI

Sequences:

>Translated_498_residues
MLHLFAGLDLHTGLLLLLALAFVLFYEAINGFHDTANAVATVIYTRAMRSQLAVAMAALFNFFGVLLGGLSVAYAIVHML
PTDLLLNMGSAHGLAMVFSMLLAAIIWNLGTWYFGLPASSSHTLIGAIIGIGLTNALMTGTSVVDALNIPKVIGIFASLI
ISPIVGLVIAGGLIFLLRRYWSGTKKRARIHLTPAEREKKDGKKKPPFWTRIALILSAIGVSFSHGANDGQKGIGLVMLV
LIGVAPAGFVVNMNASGYEITRTRDAVNNVETFFQQRPELLKKATGVDQLVPSPDTNTAANGEFHCHPANTINALDRVKT
MLTGVENYESLKPEQRGQLRRIMLCISDTTDKVAKLPDVSADDQRLLKKLKTDMLSTIEYAPIWIIMAVALALGIGTMIG
WRRVATTIGEKIGKKGMTYAQGMSAQMTAAVSIGLASYTGMPVSTTHVLSSSVAGTMLVDGGGLQKKTVTSILMAWVLTL
PAAIILSGVLYWLSLKLI
>Mature_498_residues
MLHLFAGLDLHTGLLLLLALAFVLFYEAINGFHDTANAVATVIYTRAMRSQLAVAMAALFNFFGVLLGGLSVAYAIVHML
PTDLLLNMGSAHGLAMVFSMLLAAIIWNLGTWYFGLPASSSHTLIGAIIGIGLTNALMTGTSVVDALNIPKVIGIFASLI
ISPIVGLVIAGGLIFLLRRYWSGTKKRARIHLTPAEREKKDGKKKPPFWTRIALILSAIGVSFSHGANDGQKGIGLVMLV
LIGVAPAGFVVNMNASGYEITRTRDAVNNVETFFQQRPELLKKATGVDQLVPSPDTNTAANGEFHCHPANTINALDRVKT
MLTGVENYESLKPEQRGQLRRIMLCISDTTDKVAKLPDVSADDQRLLKKLKTDMLSTIEYAPIWIIMAVALALGIGTMIG
WRRVATTIGEKIGKKGMTYAQGMSAQMTAAVSIGLASYTGMPVSTTHVLSSSVAGTMLVDGGGLQKKTVTSILMAWVLTL
PAAIILSGVLYWLSLKLI

Specific function: Low-affinity inorganic phosphate transport. Can also transport arsenate [H]

COG id: COG0306

COG function: function code P; Phosphate/sulphate permeases

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the inorganic phosphate transporter (PiT) (TC 2.A.20) family. Pit subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789907, Length=499, Percent_Identity=88.376753507014, Blast_Score=865, Evalue=0.0,
Organism=Escherichia coli, GI1789360, Length=499, Percent_Identity=80.9619238476954, Blast_Score=779, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001204 [H]

Pfam domain/function: PF01384 PHO4 [H]

EC number: NA

Molecular weight: Translated: 53362; Mature: 53362

Theoretical pI: Translated: 10.14; Mature: 10.14

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLHLFAGLDLHTGLLLLLALAFVLFYEAINGFHDTANAVATVIYTRAMRSQLAVAMAALF
CEEEEECCCHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NFFGVLLGGLSVAYAIVHMLPTDLLLNMGSAHGLAMVFSMLLAAIIWNLGTWYFGLPASS
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCEEECCCCC
SHTLIGAIIGIGLTNALMTGTSVVDALNIPKVIGIFASLIISPIVGLVIAGGLIFLLRRY
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
WSGTKKRARIHLTPAEREKKDGKKKPPFWTRIALILSAIGVSFSHGANDGQKGIGLVMLV
HCCCCCCEEEEECCCHHHHHCCCCCCCHHHHHHHHHHHHCCHHCCCCCCCCHHHHHHHHH
LIGVAPAGFVVNMNASGYEITRTRDAVNNVETFFQQRPELLKKATGVDQLVPSPDTNTAA
HHHCCCCCEEEEECCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHCCCHHCCCCCCCCCCC
NGEFHCHPANTINALDRVKTMLTGVENYESLKPEQRGQLRRIMLCISDTTDKVAKLPDVS
CCCEEECCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCHHHHHHCCCCC
ADDQRLLKKLKTDMLSTIEYAPIWIIMAVALALGIGTMIGWRRVATTIGEKIGKKGMTYA
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHH
QGMSAQMTAAVSIGLASYTGMPVSTTHVLSSSVAGTMLVDGGGLQKKTVTSILMAWVLTL
CCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCEEEECCCCCCHHHHHHHHHHHHHHH
PAAIILSGVLYWLSLKLI
HHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MLHLFAGLDLHTGLLLLLALAFVLFYEAINGFHDTANAVATVIYTRAMRSQLAVAMAALF
CEEEEECCCHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NFFGVLLGGLSVAYAIVHMLPTDLLLNMGSAHGLAMVFSMLLAAIIWNLGTWYFGLPASS
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCEEECCCCC
SHTLIGAIIGIGLTNALMTGTSVVDALNIPKVIGIFASLIISPIVGLVIAGGLIFLLRRY
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
WSGTKKRARIHLTPAEREKKDGKKKPPFWTRIALILSAIGVSFSHGANDGQKGIGLVMLV
HCCCCCCEEEEECCCHHHHHCCCCCCCHHHHHHHHHHHHCCHHCCCCCCCCHHHHHHHHH
LIGVAPAGFVVNMNASGYEITRTRDAVNNVETFFQQRPELLKKATGVDQLVPSPDTNTAA
HHHCCCCCEEEEECCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHCCCHHCCCCCCCCCCC
NGEFHCHPANTINALDRVKTMLTGVENYESLKPEQRGQLRRIMLCISDTTDKVAKLPDVS
CCCEEECCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCHHHHHHCCCCC
ADDQRLLKKLKTDMLSTIEYAPIWIIMAVALALGIGTMIGWRRVATTIGEKIGKKGMTYA
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHH
QGMSAQMTAAVSIGLASYTGMPVSTTHVLSSSVAGTMLVDGGGLQKKTVTSILMAWVLTL
CCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCEEEECCCCCCHHHHHHHHHHHHHHH
PAAIILSGVLYWLSLKLI
HHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; phosphate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + phosphate [Periplasm] = Proton [Cytoplasm] + phosphate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]