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The map label for this gene is glgX [H]

Identifier: 238896893

GI number: 238896893

Start: 4865857

End: 4867833

Strand: Reverse

Name: glgX [H]

Synonym: KP1_5130

Alternate gene names: 238896893

Gene position: 4867833-4865857 (Counterclockwise)

Preceding gene: 238896894

Following gene: 238896892

Centisome position: 92.75

GC content: 62.22

Gene sequence:

>1977_bases
ATGACCTCACTCGCGGCAGGCAAACCTGCGCCATTGGGCGCCAGCTATGACGGCAAGGGGGTGAATTTCGCCCTCTTCTC
CGCCCATGCGGAGCGGGTGGAGCTGTGCGTATTCGACGAGCAGGGCAACGAGCAACGCTTCGATCTCCCGGCGCGCAGCG
GCGACATCTGGCACGGCTGGCTGGCCGCCGCCGGGCCGGGCCTGCGCTACGGCTATCGGGTACATGGTCCCTGGGACCCG
GCGCAGGGGCACCGCTTTAACCCGGCCAAGCTGTTGATCGATCCCAGCGCCCACCGGGTCGAGGGCGATCTGCCTGACGA
TGAGCGCCTGCACGGCGGCATGTGGCAGCCCGATCGCCGCGACAGCGCCGCGGTCGCGCCAAAATCGCAGGTGGTGGATC
TGCGCTATGACTGGCGTGGCGATAAACCGCCGCGTACCCCATGGGGGGAAACGGTGATTTATGAAGCGCACGTGAAAGGG
CTCACCTTGCTTAATCCGCAGCTACCGGAAGCCATTCGCGGAACCTATAAAGCGCTGGGCCACCCGGCGATGATCGCTTA
CTTCAAATCGCTGGGCATCAGCGCCCTTGAGCTGTTGCCGGTGGCGCAGTTCGCCAGCGAGCCGCGACTGCAGCGGATGG
GGTTAAGCAACTACTGGGGCTACAACCCGTTGGCGTGGTTCGCGCTGGATCCGCGCTATGCCAGCGACCCGGATCGCGCC
CTCGATGAGTTTCGCGATGCGGTTAAGGCGCTGCACGCCGCCGGTATCGAAGTGATTCTGGATATCGTGCTCAACCATAG
TGCGGAAATCGATCTAGAAGGGCCCACCGTCTCCCTGCGCGGAATCGACAACCGTAGCTATTATTGGGTAAGGGAGGATG
GCGATTATCACAACTGGACCGGGTGCGGTAATACGCTCAATCTTAGCCACCCCGGCGTGGTGGAGTGGGCGCGCCAGTGT
TTGCGCTTTTGGGTGGATGAGTGCCATGTCGACGGCTTTCGCTTCGACCTCGCCTCAGTGATGGGGCGGACGCCGGAATT
CCGCCAGGGTGCGCCGCTGTTCGAGGCTATCCGCCGCGACAGCGTGCTGTCGCAGGTGAAACTGATTGCGGAACCCTGGG
ATATCGGCCCGGGCGGCTATCAGGTGGGTAATTTCCCACCGCTGTTCGCTGAATGGAATGACCATTTTCGCGATAGCGCC
CGACGCTTCTGGTTACAGCAAAATGTCTCCCTGGGGGATTTTGCCCAACGTTTCGCGGCCTCCAGCGATCTCTTTGCCCG
CGATGGCAAGCCGCCATCGGCGACGGTCAACCTGGTGACGGCCCACGATGGCTTCACGCTGCGCGACTGCGTTTGTTTCA
ATCAGAAACACAATGAGGCAAACGGGGAGGAGAATCGCGATGGGACTAACAATAACTACAGCAACAATCATGGTATAGAA
GGATTAGAAGCGAATTTTGCGGTGATTGAACGGCGGCGCGCCAGCGCGCATGCGCTGCTGACGACGCTGCTGCTGGCTCA
GGGGACGCCAATGCTGCTGGCGGGCGATGAACAGGGCCACAGCCAGCACGGTAACAATAATGCTTACTGCCAGGACAACG
CCCTGACGTGGCTGGACTGGCGCCAGGCGAACCCGGGATTAACCGCCTTTACCGCGGCGCTGATCCACCTGCGGCGCCGT
ATTCCGGCGCTCACCCGCAACCGCTGGTGGCAGGAGGGGGATGGCAACGTCCGCTGGCTTAATCGCAACGCCCAGCCCCT
GACTGCCGCGGAGTGGCAGCAGGGGGCCGCGTGTATGCAGATCCAGTTGTCGGATCGCTGGCTGCTCACGCTGAACGCCA
CCGCAGAGGTGGTGGATATGGTTTTACCCGAGGGGGAATGGCGCGCCGTTCCTCCATTCGCCGGAGAGGATAATCCGGTC
ATCATGGCTGTCTGGCATGGGCCCGCGCACGGAGTGTGCGTATTTCAAAGGTCGTAA

Upstream 100 bases:

>100_bases
GCGTGGTGGAAAGCGACGCTATCGCCAGCCATGGCCGCGAACATTCGCTGTCGCTGACCCTGCCCCCGCTGGCGACGATC
TGGCTGGTCCGGGAGGCGCA

Downstream 100 bases:

>100_bases
GCCCTGAAGCTTTCGGTTGGACCCGTGGCGGCGGCGCGGCGGGATGAGCTGGAAAGCGGGGGTGTAAAAAGGAGTTAATC
ATGGTTAGGCTTGAAAAAAA

Product: glycogen debranching enzyme

Products: NA

Alternate protein names: Glycogen operon protein GlgX [H]

Number of amino acids: Translated: 658; Mature: 657

Protein sequence:

>658_residues
MTSLAAGKPAPLGASYDGKGVNFALFSAHAERVELCVFDEQGNEQRFDLPARSGDIWHGWLAAAGPGLRYGYRVHGPWDP
AQGHRFNPAKLLIDPSAHRVEGDLPDDERLHGGMWQPDRRDSAAVAPKSQVVDLRYDWRGDKPPRTPWGETVIYEAHVKG
LTLLNPQLPEAIRGTYKALGHPAMIAYFKSLGISALELLPVAQFASEPRLQRMGLSNYWGYNPLAWFALDPRYASDPDRA
LDEFRDAVKALHAAGIEVILDIVLNHSAEIDLEGPTVSLRGIDNRSYYWVREDGDYHNWTGCGNTLNLSHPGVVEWARQC
LRFWVDECHVDGFRFDLASVMGRTPEFRQGAPLFEAIRRDSVLSQVKLIAEPWDIGPGGYQVGNFPPLFAEWNDHFRDSA
RRFWLQQNVSLGDFAQRFAASSDLFARDGKPPSATVNLVTAHDGFTLRDCVCFNQKHNEANGEENRDGTNNNYSNNHGIE
GLEANFAVIERRRASAHALLTTLLLAQGTPMLLAGDEQGHSQHGNNNAYCQDNALTWLDWRQANPGLTAFTAALIHLRRR
IPALTRNRWWQEGDGNVRWLNRNAQPLTAAEWQQGAACMQIQLSDRWLLTLNATAEVVDMVLPEGEWRAVPPFAGEDNPV
IMAVWHGPAHGVCVFQRS

Sequences:

>Translated_658_residues
MTSLAAGKPAPLGASYDGKGVNFALFSAHAERVELCVFDEQGNEQRFDLPARSGDIWHGWLAAAGPGLRYGYRVHGPWDP
AQGHRFNPAKLLIDPSAHRVEGDLPDDERLHGGMWQPDRRDSAAVAPKSQVVDLRYDWRGDKPPRTPWGETVIYEAHVKG
LTLLNPQLPEAIRGTYKALGHPAMIAYFKSLGISALELLPVAQFASEPRLQRMGLSNYWGYNPLAWFALDPRYASDPDRA
LDEFRDAVKALHAAGIEVILDIVLNHSAEIDLEGPTVSLRGIDNRSYYWVREDGDYHNWTGCGNTLNLSHPGVVEWARQC
LRFWVDECHVDGFRFDLASVMGRTPEFRQGAPLFEAIRRDSVLSQVKLIAEPWDIGPGGYQVGNFPPLFAEWNDHFRDSA
RRFWLQQNVSLGDFAQRFAASSDLFARDGKPPSATVNLVTAHDGFTLRDCVCFNQKHNEANGEENRDGTNNNYSNNHGIE
GLEANFAVIERRRASAHALLTTLLLAQGTPMLLAGDEQGHSQHGNNNAYCQDNALTWLDWRQANPGLTAFTAALIHLRRR
IPALTRNRWWQEGDGNVRWLNRNAQPLTAAEWQQGAACMQIQLSDRWLLTLNATAEVVDMVLPEGEWRAVPPFAGEDNPV
IMAVWHGPAHGVCVFQRS
>Mature_657_residues
TSLAAGKPAPLGASYDGKGVNFALFSAHAERVELCVFDEQGNEQRFDLPARSGDIWHGWLAAAGPGLRYGYRVHGPWDPA
QGHRFNPAKLLIDPSAHRVEGDLPDDERLHGGMWQPDRRDSAAVAPKSQVVDLRYDWRGDKPPRTPWGETVIYEAHVKGL
TLLNPQLPEAIRGTYKALGHPAMIAYFKSLGISALELLPVAQFASEPRLQRMGLSNYWGYNPLAWFALDPRYASDPDRAL
DEFRDAVKALHAAGIEVILDIVLNHSAEIDLEGPTVSLRGIDNRSYYWVREDGDYHNWTGCGNTLNLSHPGVVEWARQCL
RFWVDECHVDGFRFDLASVMGRTPEFRQGAPLFEAIRRDSVLSQVKLIAEPWDIGPGGYQVGNFPPLFAEWNDHFRDSAR
RFWLQQNVSLGDFAQRFAASSDLFARDGKPPSATVNLVTAHDGFTLRDCVCFNQKHNEANGEENRDGTNNNYSNNHGIEG
LEANFAVIERRRASAHALLTTLLLAQGTPMLLAGDEQGHSQHGNNNAYCQDNALTWLDWRQANPGLTAFTAALIHLRRRI
PALTRNRWWQEGDGNVRWLNRNAQPLTAAEWQQGAACMQIQLSDRWLLTLNATAEVVDMVLPEGEWRAVPPFAGEDNPVI
MAVWHGPAHGVCVFQRS

Specific function: Hydrolyzes the alpha-1,6-glucosidic linkages in glycogen which has first been partially depolymerized by phosphorylase. Shows only very little activity with native glycogen [H]

COG id: COG1523

COG function: function code G; Type II secretory pathway, pullulanase PulA and related glycosidases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 13 family [H]

Homologues:

Organism=Escherichia coli, GI2367229, Length=657, Percent_Identity=77.4733637747336, Blast_Score=1073, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006047
- InterPro:   IPR004193
- InterPro:   IPR017853
- InterPro:   IPR013781
- InterPro:   IPR022844
- InterPro:   IPR011837
- InterPro:   IPR013783
- InterPro:   IPR014756 [H]

Pfam domain/function: PF00128 Alpha-amylase; PF02922 CBM_48 [H]

EC number: 3.2.1.- [C]

Molecular weight: Translated: 73563; Mature: 73432

Theoretical pI: Translated: 6.10; Mature: 6.10

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSLAAGKPAPLGASYDGKGVNFALFSAHAERVELCVFDEQGNEQRFDLPARSGDIWHGW
CCCCCCCCCCCCCCCCCCCCCEEEEEECCCCEEEEEEECCCCCCCEECCCCCCCCCCCHH
LAAAGPGLRYGYRVHGPWDPAQGHRFNPAKLLIDPSAHRVEGDLPDDERLHGGMWQPDRR
HHHCCCCCCCCEEECCCCCCCCCCCCCCCEEEECCCCCCCCCCCCCCHHHCCCCCCCCCC
DSAAVAPKSQVVDLRYDWRGDKPPRTPWGETVIYEAHVKGLTLLNPQLPEAIRGTYKALG
CCCCCCCCCEEEEEEECCCCCCCCCCCCCCEEEEEECCCCEEEECCCCCHHHHHHHHHHC
HPAMIAYFKSLGISALELLPVAQFASEPRLQRMGLSNYWGYNPLAWFALDPRYASDPDRA
CHHHHHHHHHCCCHHHHHHHHHHHHCCCHHHHCCCCCCCCCCCEEEEEECCCCCCCHHHH
LDEFRDAVKALHAAGIEVILDIVLNHSAEIDLEGPTVSLRGIDNRSYYWVREDGDYHNWT
HHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCEEEEEECCCCEEEEEECCCCCCCCC
GCGNTLNLSHPGVVEWARQCLRFWVDECHVDGFRFDLASVMGRTPEFRQGAPLFEAIRRD
CCCCEECCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHH
SVLSQVKLIAEPWDIGPGGYQVGNFPPLFAEWNDHFRDSARRFWLQQNVSLGDFAQRFAA
HHHHHHHHHCCCCCCCCCCEECCCCCCCHHCCCHHHHHHHHHHHHHCCCCHHHHHHHHHC
SSDLFARDGKPPSATVNLVTAHDGFTLRDCVCFNQKHNEANGEENRDGTNNNYSNNHGIE
CCCCHHCCCCCCCCEEEEEEECCCCEEEHEEEECCCCCCCCCCCCCCCCCCCCCCCCCCC
GLEANFAVIERRRASAHALLTTLLLAQGTPMLLAGDEQGHSQHGNNNAYCQDNALTWLDW
CCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCEECCCCEEEEEE
RQANPGLTAFTAALIHLRRRIPALTRNRWWQEGDGNVRWLNRNAQPLTAAEWQQGAACMQ
ECCCCCHHHHHHHHHHHHHHCCHHHHHCCCCCCCCCEEEECCCCCCCCHHHHHCCCEEEE
IQLSDRWLLTLNATAEVVDMVLPEGEWRAVPPFAGEDNPVIMAVWHGPAHGVCVFQRS
EEECCEEEEEECCHHHHHHHHCCCCCCEECCCCCCCCCCEEEEEEECCCCCEEEEECC
>Mature Secondary Structure 
TSLAAGKPAPLGASYDGKGVNFALFSAHAERVELCVFDEQGNEQRFDLPARSGDIWHGW
CCCCCCCCCCCCCCCCCCCCEEEEEECCCCEEEEEEECCCCCCCEECCCCCCCCCCCHH
LAAAGPGLRYGYRVHGPWDPAQGHRFNPAKLLIDPSAHRVEGDLPDDERLHGGMWQPDRR
HHHCCCCCCCCEEECCCCCCCCCCCCCCCEEEECCCCCCCCCCCCCCHHHCCCCCCCCCC
DSAAVAPKSQVVDLRYDWRGDKPPRTPWGETVIYEAHVKGLTLLNPQLPEAIRGTYKALG
CCCCCCCCCEEEEEEECCCCCCCCCCCCCCEEEEEECCCCEEEECCCCCHHHHHHHHHHC
HPAMIAYFKSLGISALELLPVAQFASEPRLQRMGLSNYWGYNPLAWFALDPRYASDPDRA
CHHHHHHHHHCCCHHHHHHHHHHHHCCCHHHHCCCCCCCCCCCEEEEEECCCCCCCHHHH
LDEFRDAVKALHAAGIEVILDIVLNHSAEIDLEGPTVSLRGIDNRSYYWVREDGDYHNWT
HHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCEEEEEECCCCEEEEEECCCCCCCCC
GCGNTLNLSHPGVVEWARQCLRFWVDECHVDGFRFDLASVMGRTPEFRQGAPLFEAIRRD
CCCCEECCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHH
SVLSQVKLIAEPWDIGPGGYQVGNFPPLFAEWNDHFRDSARRFWLQQNVSLGDFAQRFAA
HHHHHHHHHCCCCCCCCCCEECCCCCCCHHCCCHHHHHHHHHHHHHCCCCHHHHHHHHHC
SSDLFARDGKPPSATVNLVTAHDGFTLRDCVCFNQKHNEANGEENRDGTNNNYSNNHGIE
CCCCHHCCCCCCCCEEEEEEECCCCEEEHEEEECCCCCCCCCCCCCCCCCCCCCCCCCCC
GLEANFAVIERRRASAHALLTTLLLAQGTPMLLAGDEQGHSQHGNNNAYCQDNALTWLDW
CCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCEECCCCEEEEEE
RQANPGLTAFTAALIHLRRRIPALTRNRWWQEGDGNVRWLNRNAQPLTAAEWQQGAACMQ
ECCCCCHHHHHHHHHHHHHHCCHHHHHCCCCCCCCCEEEECCCCCCCCHHHHHCCCEEEE
IQLSDRWLLTLNATAEVVDMVLPEGEWRAVPPFAGEDNPVIMAVWHGPAHGVCVFQRS
EEECCEEEEEECCHHHHHHHHCCCCCCEECCCCCCCCCCEEEEEEECCCCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Glycosylases; Glycosidases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA