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The map label for this gene is bioH [H]

Identifier: 238896879

GI number: 238896879

Start: 4845046

End: 4845819

Strand: Reverse

Name: bioH [H]

Synonym: KP1_5114

Alternate gene names: 238896879

Gene position: 4845819-4845046 (Counterclockwise)

Preceding gene: 238896883

Following gene: 238896878

Centisome position: 92.33

GC content: 64.99

Gene sequence:

>774_bases
ATGAACGACATCTGGTGGCAAACAATCGGCGAAGGAGATTGTCATCTTGTGCTGCTGCACGGCTGGGGGCTGAACGCTCA
GGTATGGGATTGCATCACGCCGCAGCTGGCTTCGCATTTTACCCTCCACCTGGTGGATCTGCCGGGCTATGGCCGCAGCG
GCGGGTTTGGGGCGATGTCGCTGGAGGCGATGGCGCAGCGGGTGCTGGAGCAGGCCCCTCCACAGGCGGTGTGGCTGGGC
TGGAGCCTGGGCGGGCTGGTGGCCAGCCAGGTCGCGATTATGCGTCCGGAACGCGTGCAGGCGCTGGTGACGGTTGCCTC
TTCCCCCTGTTTCGCGGCTCGTGACGACTGGCCCGGCATCAAGCCCGAGGTGCTGGCCGGATTTCAGCAGCAGCTGAGCG
ACGATTTCCAGCGCACCGTCGAGCGATTCCTCGCCCTGCAAACCATGGGCACAGAGAGCGCGCGTCAGGATGCGCGGGCG
TTAAAGCAGGCGGTGCTCTCGCTGCCGATGCCTTCCGCCGAGGCGCTGAATGGCGGACTGGAGATCCTCAGAACCGTCGA
TCTGCGTCAGGCGCTGGTCAGGCTGCCGATGCCGTTTCTGCGGCTGTATGGCCGGCTGGATGGACTGGTGCCGCGCAAGA
TCGTCCCGTTGCTGGATGACCTGTGGCCGGAAAGCGAGTCAATCCTCTTCGATAAGGCCGCGCATGCGCCGTTTGTTTCC
CATCCCGCGGCCTTCTGCGAGCCGCTGCTGGCGCTGAAGACGCGGCTGGGGTAA

Upstream 100 bases:

>100_bases
CCCCAGCGGGCGACTGCCAGCGGCATTTGGCATAGCCAGCATAAGCTGTGTGCTGTTAGCATATTTGGCCTTCTGTCCAG
TAAAAAGAGAACGGTAACGG

Downstream 100 bases:

>100_bases
TTTTTTTTGCCGCGCCATGGTAAAAGGGCCATTCCTAGCAATACTTAGGTTGTTACGGTGGTTGATTATTTCAATCCGCC
ACCGTGACCTACAATAACAA

Product: biotin biosynthetic protein

Products: NA

Alternate protein names: Biotin synthesis protein BioH [H]

Number of amino acids: Translated: 257; Mature: 257

Protein sequence:

>257_residues
MNDIWWQTIGEGDCHLVLLHGWGLNAQVWDCITPQLASHFTLHLVDLPGYGRSGGFGAMSLEAMAQRVLEQAPPQAVWLG
WSLGGLVASQVAIMRPERVQALVTVASSPCFAARDDWPGIKPEVLAGFQQQLSDDFQRTVERFLALQTMGTESARQDARA
LKQAVLSLPMPSAEALNGGLEILRTVDLRQALVRLPMPFLRLYGRLDGLVPRKIVPLLDDLWPESESILFDKAAHAPFVS
HPAAFCEPLLALKTRLG

Sequences:

>Translated_257_residues
MNDIWWQTIGEGDCHLVLLHGWGLNAQVWDCITPQLASHFTLHLVDLPGYGRSGGFGAMSLEAMAQRVLEQAPPQAVWLG
WSLGGLVASQVAIMRPERVQALVTVASSPCFAARDDWPGIKPEVLAGFQQQLSDDFQRTVERFLALQTMGTESARQDARA
LKQAVLSLPMPSAEALNGGLEILRTVDLRQALVRLPMPFLRLYGRLDGLVPRKIVPLLDDLWPESESILFDKAAHAPFVS
HPAAFCEPLLALKTRLG
>Mature_257_residues
MNDIWWQTIGEGDCHLVLLHGWGLNAQVWDCITPQLASHFTLHLVDLPGYGRSGGFGAMSLEAMAQRVLEQAPPQAVWLG
WSLGGLVASQVAIMRPERVQALVTVASSPCFAARDDWPGIKPEVLAGFQQQLSDDFQRTVERFLALQTMGTESARQDARA
LKQAVLSLPMPSAEALNGGLEILRTVDLRQALVRLPMPFLRLYGRLDGLVPRKIVPLLDDLWPESESILFDKAAHAPFVS
HPAAFCEPLLALKTRLG

Specific function: Shows carboxylesterase activity with a preference for short chain fatty acid esters (acyl chain length of up to 6 carbons). Also displays a weak thioesterase activity. Can form a complex with CoA, and may be involved in the condensation of CoA and pimelic

COG id: COG0596

COG function: function code R; Predicted hydrolases or acyltransferases (alpha/beta hydrolase superfamily)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AB hydrolase superfamily. Carboxylesterase BioH family [H]

Homologues:

Organism=Escherichia coli, GI1789817, Length=256, Percent_Identity=77.34375, Blast_Score=418, Evalue=1e-118,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000073
- InterPro:   IPR010076 [H]

Pfam domain/function: PF00561 Abhydrolase_1 [H]

EC number: =3.1.1.1 [H]

Molecular weight: Translated: 28264; Mature: 28264

Theoretical pI: Translated: 5.94; Mature: 5.94

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNDIWWQTIGEGDCHLVLLHGWGLNAQVWDCITPQLASHFTLHLVDLPGYGRSGGFGAMS
CCCCCCCCCCCCCEEEEEEECCCCCCHHHHHCCHHHHCCEEEEEEECCCCCCCCCCCHHH
LEAMAQRVLEQAPPQAVWLGWSLGGLVASQVAIMRPERVQALVTVASSPCFAARDDWPGI
HHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCCCC
KPEVLAGFQQQLSDDFQRTVERFLALQTMGTESARQDARALKQAVLSLPMPSAEALNGGL
CHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHH
EILRTVDLRQALVRLPMPFLRLYGRLDGLVPRKIVPLLDDLWPESESILFDKAAHAPFVS
HHHHHHHHHHHHHHCCHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCEEHHHHHCCCCCC
HPAAFCEPLLALKTRLG
CCHHHHHHHHHHHHCCC
>Mature Secondary Structure
MNDIWWQTIGEGDCHLVLLHGWGLNAQVWDCITPQLASHFTLHLVDLPGYGRSGGFGAMS
CCCCCCCCCCCCCEEEEEEECCCCCCHHHHHCCHHHHCCEEEEEEECCCCCCCCCCCHHH
LEAMAQRVLEQAPPQAVWLGWSLGGLVASQVAIMRPERVQALVTVASSPCFAARDDWPGI
HHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCCCC
KPEVLAGFQQQLSDDFQRTVERFLALQTMGTESARQDARALKQAVLSLPMPSAEALNGGL
CHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHH
EILRTVDLRQALVRLPMPFLRLYGRLDGLVPRKIVPLLDDLWPESESILFDKAAHAPFVS
HHHHHHHHHHHHHHCCHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCEEHHHHHCCCCCC
HPAAFCEPLLALKTRLG
CCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA