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The map label for this gene is kefB [H]

Identifier: 238896833

GI number: 238896833

Start: 4795198

End: 4797003

Strand: Reverse

Name: kefB [H]

Synonym: KP1_5064

Alternate gene names: 238896833

Gene position: 4797003-4795198 (Counterclockwise)

Preceding gene: 238896834

Following gene: 238896832

Centisome position: 91.4

GC content: 59.97

Gene sequence:

>1806_bases
ATGGCGGGATCTGATTTGCTGCTGGCGGGGGTGTTGTTTTTGTTCGCGGCCGTCATCGCCGTCCCTCTGGCTTCAAGACT
GGGGATTGGCGCGGTGCTGGGCTATCTGCTGGCCGGGATCGCCATTGGTCCGTGGGGGCTCGGGTTTATCAGCGATGTGG
ATGAAATTCTCCATTTCTCTGAGCTCGGCGTAGTGTTTCTGATGTTTATCATCGGCCTGGAGCTCAATCCCGCCAAGCTA
TGGCGACTGCGGAGCTCCATCTTCGGCGTTGGCGCGGCGCAGGTGATGCTCAGCGCGGCGATCCTCGGTGGGTTGTTGAT
GACGACCGGGTTTTCATGGCAGGCGGCGGTGGTCGGCGGGATCGGCCTGGCGATGTCTTCCACCGCCATGGCGCTGCAGC
TGATGCGTGAGAAGGGAATGAGCCGCAGCGAAGCGGGACAGCTAGGCTTTTCGGTGCTGCTGTTTCAGGATCTGGCGGTG
ATCCCGGCGCTGGCGCTGGTGCCCCTGCTGGCCGGTTCAGCGGATGAGCATGTGAACTGGTTGACGGTCGGCATGAAGGT
CCTGGCCTTTGCCGGGATGCTGATTGGCGGACGCTATCTGCTGCGGCCGGTGTTCCGTTTTATCGCCAGCTCGGGGGTCA
GGGAGGTGTTCACTGCCGCCACGCTGCTGTTGGTCCTCGGATCTGCCCTGTTTATGGAGGCGCTGGGGCTGTCGATGGCG
CTGGGCACCTTTATTGCCGGCGTGCTGCTGGCGGAAAGTGAGTATCGCCACGAACTGGAGATCGCTATCGATCCGTTCAA
GGGGCTGCTGCTCGGGTTGTTCTTTATTTCCGTCGGCATGGCGCTGAACCTTGGCGTACTGTATACCCATCTGCTGTGGG
TGGCGGTGAGCGTGGCGGTGCTGGTGGCGGTAAAAATGCTGGTGCTCTATCTGCTGGCCCGTCTCTATGGCCTGCGAAGT
TCCGAGCGGATGCAGTTCGCGGGCGTATTAAGCCAGGGCGGCGAGTTCGCGTTTGTCCTCTTTTCGCTGCCCGCCTCTCA
GCGGTTGTTTCAGCACGACCAGATGGCGCTGCTGCTGGTGGCGGTAACGTTGTCGATGATGACGACGCCGCTGCTGATGA
AGGGGATAGATAAGCTATTGTCGCGACGGCTGAACCCGGCGGACGATACCGGTGAGGCGCCGTGGGTGGAGGATGACAAG
CCGCAGGTGATTATTGTCGGGTTTGGTCGCTTTGGTCAGGTCATTGGTCGCCTGCTGATGGCCAATAAGATGCGTATTAC
GGTACTGGAGCGGGACATCAGCGCGGTAAATCTGATGCGCAACTATGGCTATAAAGTCTATTTTGGCGATGCCACCCAGC
TGGAGCTGCTGCGCTCGGCGGGAGCGGAAGAGGCGCAGTCGATCGTCATCACCTGTAATGAGCCAGAAGATACCATGCGG
CTGGTGGAAATGTGTCAACAACACTTTCCACATCTGCATATTCTGGCCCGCGCTCGCGGGCGCGTTGAGGCGCATGAGCT
GCTGCAGGCCGGCGTGACGCAGTTTTCTCGTGAAACTTTCTCCAGTGCGTTGGAACTGGGGCGCAAAGCGCTCATCACCC
TCGGGATGCATCCTCACCAGGCCCAGCGCGCGCAGCTGCACTTCCGACGCCTCGATATGCGAATGCTGCGCGAGCTAATG
CCAGTGCATACTGATACGGTACAGATATCACGCGTGCGGGAGGCCCGGCGCGAACTGGAAGAGATTTTCCAGCGCGAAAT
GCAGAAAGAGAGCCGGCAACTGGACGGCTGGGACGAGTTTGAATAA

Upstream 100 bases:

>100_bases
ATCGTCTACTGGGCCCGGAGGCAGCAGCCAGAGGAGCTGCGCAGCCGCGCCCTGGCCTACCGGGACTGGTTGGCCAACCC
CATCGCCGCCGGAGGTGTCC

Downstream 100 bases:

>100_bases
AGGTAAATCTTATGGCAGTGCGTAAACGTTTTATTGCCGGGGCGAAATGTCCGGCGTGCCAGGCGCAGGATTCGCTGGCA
ATGTGGCGTGAGAATAATGT

Product: glutathione-regulated potassium-efflux system protein KefB

Products: Proton [Cytoplasm]; K (I) [Periplasm] [C]

Alternate protein names: K(+)/H(+) antiporter; NEM-activatable K(+)/H(+) antiporter [H]

Number of amino acids: Translated: 601; Mature: 600

Protein sequence:

>601_residues
MAGSDLLLAGVLFLFAAVIAVPLASRLGIGAVLGYLLAGIAIGPWGLGFISDVDEILHFSELGVVFLMFIIGLELNPAKL
WRLRSSIFGVGAAQVMLSAAILGGLLMTTGFSWQAAVVGGIGLAMSSTAMALQLMREKGMSRSEAGQLGFSVLLFQDLAV
IPALALVPLLAGSADEHVNWLTVGMKVLAFAGMLIGGRYLLRPVFRFIASSGVREVFTAATLLLVLGSALFMEALGLSMA
LGTFIAGVLLAESEYRHELEIAIDPFKGLLLGLFFISVGMALNLGVLYTHLLWVAVSVAVLVAVKMLVLYLLARLYGLRS
SERMQFAGVLSQGGEFAFVLFSLPASQRLFQHDQMALLLVAVTLSMMTTPLLMKGIDKLLSRRLNPADDTGEAPWVEDDK
PQVIIVGFGRFGQVIGRLLMANKMRITVLERDISAVNLMRNYGYKVYFGDATQLELLRSAGAEEAQSIVITCNEPEDTMR
LVEMCQQHFPHLHILARARGRVEAHELLQAGVTQFSRETFSSALELGRKALITLGMHPHQAQRAQLHFRRLDMRMLRELM
PVHTDTVQISRVREARRELEEIFQREMQKESRQLDGWDEFE

Sequences:

>Translated_601_residues
MAGSDLLLAGVLFLFAAVIAVPLASRLGIGAVLGYLLAGIAIGPWGLGFISDVDEILHFSELGVVFLMFIIGLELNPAKL
WRLRSSIFGVGAAQVMLSAAILGGLLMTTGFSWQAAVVGGIGLAMSSTAMALQLMREKGMSRSEAGQLGFSVLLFQDLAV
IPALALVPLLAGSADEHVNWLTVGMKVLAFAGMLIGGRYLLRPVFRFIASSGVREVFTAATLLLVLGSALFMEALGLSMA
LGTFIAGVLLAESEYRHELEIAIDPFKGLLLGLFFISVGMALNLGVLYTHLLWVAVSVAVLVAVKMLVLYLLARLYGLRS
SERMQFAGVLSQGGEFAFVLFSLPASQRLFQHDQMALLLVAVTLSMMTTPLLMKGIDKLLSRRLNPADDTGEAPWVEDDK
PQVIIVGFGRFGQVIGRLLMANKMRITVLERDISAVNLMRNYGYKVYFGDATQLELLRSAGAEEAQSIVITCNEPEDTMR
LVEMCQQHFPHLHILARARGRVEAHELLQAGVTQFSRETFSSALELGRKALITLGMHPHQAQRAQLHFRRLDMRMLRELM
PVHTDTVQISRVREARRELEEIFQREMQKESRQLDGWDEFE
>Mature_600_residues
AGSDLLLAGVLFLFAAVIAVPLASRLGIGAVLGYLLAGIAIGPWGLGFISDVDEILHFSELGVVFLMFIIGLELNPAKLW
RLRSSIFGVGAAQVMLSAAILGGLLMTTGFSWQAAVVGGIGLAMSSTAMALQLMREKGMSRSEAGQLGFSVLLFQDLAVI
PALALVPLLAGSADEHVNWLTVGMKVLAFAGMLIGGRYLLRPVFRFIASSGVREVFTAATLLLVLGSALFMEALGLSMAL
GTFIAGVLLAESEYRHELEIAIDPFKGLLLGLFFISVGMALNLGVLYTHLLWVAVSVAVLVAVKMLVLYLLARLYGLRSS
ERMQFAGVLSQGGEFAFVLFSLPASQRLFQHDQMALLLVAVTLSMMTTPLLMKGIDKLLSRRLNPADDTGEAPWVEDDKP
QVIIVGFGRFGQVIGRLLMANKMRITVLERDISAVNLMRNYGYKVYFGDATQLELLRSAGAEEAQSIVITCNEPEDTMRL
VEMCQQHFPHLHILARARGRVEAHELLQAGVTQFSRETFSSALELGRKALITLGMHPHQAQRAQLHFRRLDMRMLRELMP
VHTDTVQISRVREARRELEEIFQREMQKESRQLDGWDEFE

Specific function: Transport system that facilitates potassium-efflux, possibly by potassium-proton antiport [H]

COG id: COG0475

COG function: function code P; Kef-type K+ transport systems, membrane components

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 RCK N-terminal domain [H]

Homologues:

Organism=Homo sapiens, GI157388921, Length=357, Percent_Identity=26.890756302521, Blast_Score=86, Evalue=1e-16,
Organism=Escherichia coli, GI1789749, Length=601, Percent_Identity=89.5174708818636, Blast_Score=1030, Evalue=0.0,
Organism=Escherichia coli, GI1786232, Length=604, Percent_Identity=43.2119205298013, Blast_Score=486, Evalue=1e-138,
Organism=Escherichia coli, GI1786685, Length=523, Percent_Identity=30.7839388145315, Blast_Score=191, Evalue=9e-50,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006153
- InterPro:   IPR020884
- InterPro:   IPR004771
- InterPro:   IPR016040
- InterPro:   IPR003148 [H]

Pfam domain/function: PF00999 Na_H_Exchanger; PF02254 TrkA_N [H]

EC number: NA

Molecular weight: Translated: 65961; Mature: 65830

Theoretical pI: Translated: 6.85; Mature: 6.85

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
4.8 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
4.7 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAGSDLLLAGVLFLFAAVIAVPLASRLGIGAVLGYLLAGIAIGPWGLGFISDVDEILHFS
CCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
ELGVVFLMFIIGLELNPAKLWRLRSSIFGVGAAQVMLSAAILGGLLMTTGFSWQAAVVGG
HHHHHHHHHHHCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHH
IGLAMSSTAMALQLMREKGMSRSEAGQLGFSVLLFQDLAVIPALALVPLLAGSADEHVNW
HHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHH
LTVGMKVLAFAGMLIGGRYLLRPVFRFIASSGVREVFTAATLLLVLGSALFMEALGLSMA
HHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LGTFIAGVLLAESEYRHELEIAIDPFKGLLLGLFFISVGMALNLGVLYTHLLWVAVSVAV
HHHHHHHHHHHHHHHCCEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LVAVKMLVLYLLARLYGLRSSERMQFAGVLSQGGEFAFVLFSLPASQRLFQHDQMALLLV
HHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCEEEEEEECCHHHHHHHHHHHHHHHH
AVTLSMMTTPLLMKGIDKLLSRRLNPADDTGEAPWVEDDKPQVIIVGFGRFGQVIGRLLM
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEEECCHHHHHHHHHHH
ANKMRITVLERDISAVNLMRNYGYKVYFGDATQLELLRSAGAEEAQSIVITCNEPEDTMR
HCCCEEEEEHHHHHHHHHHHHCCCEEEECCHHHHHHHHHCCCCCCCEEEEEECCCHHHHH
LVEMCQQHFPHLHILARARGRVEAHELLQAGVTQFSRETFSSALELGRKALITLGMHPHQ
HHHHHHHHCCCEEEHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHCCCCCCH
AQRAQLHFRRLDMRMLRELMPVHTDTVQISRVREARRELEEIFQREMQKESRQLDGWDEF
HHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
E
C
>Mature Secondary Structure 
AGSDLLLAGVLFLFAAVIAVPLASRLGIGAVLGYLLAGIAIGPWGLGFISDVDEILHFS
CCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
ELGVVFLMFIIGLELNPAKLWRLRSSIFGVGAAQVMLSAAILGGLLMTTGFSWQAAVVGG
HHHHHHHHHHHCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHH
IGLAMSSTAMALQLMREKGMSRSEAGQLGFSVLLFQDLAVIPALALVPLLAGSADEHVNW
HHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHH
LTVGMKVLAFAGMLIGGRYLLRPVFRFIASSGVREVFTAATLLLVLGSALFMEALGLSMA
HHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LGTFIAGVLLAESEYRHELEIAIDPFKGLLLGLFFISVGMALNLGVLYTHLLWVAVSVAV
HHHHHHHHHHHHHHHCCEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LVAVKMLVLYLLARLYGLRSSERMQFAGVLSQGGEFAFVLFSLPASQRLFQHDQMALLLV
HHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCEEEEEEECCHHHHHHHHHHHHHHHH
AVTLSMMTTPLLMKGIDKLLSRRLNPADDTGEAPWVEDDKPQVIIVGFGRFGQVIGRLLM
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEEECCHHHHHHHHHHH
ANKMRITVLERDISAVNLMRNYGYKVYFGDATQLELLRSAGAEEAQSIVITCNEPEDTMR
HCCCEEEEEHHHHHHHHHHHHCCCEEEECCHHHHHHHHHCCCCCCCEEEEEECCCHHHHH
LVEMCQQHFPHLHILARARGRVEAHELLQAGVTQFSRETFSSALELGRKALITLGMHPHQ
HHHHHHHHCCCEEEHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHCCCCCCH
AQRAQLHFRRLDMRMLRELMPVHTDTVQISRVREARRELEEIFQREMQKESRQLDGWDEF
HHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
E
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; K (I) [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + K (I) [Cytoplasm] = Proton [Cytoplasm] + K (I) [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA