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The map label for this gene is yhdG [H]

Identifier: 238896768

GI number: 238896768

Start: 4747529

End: 4748494

Strand: Direct

Name: yhdG [H]

Synonym: KP1_4988

Alternate gene names: 238896768

Gene position: 4747529-4748494 (Clockwise)

Preceding gene: 238896767

Following gene: 238896769

Centisome position: 90.45

GC content: 55.28

Gene sequence:

>966_bases
ATGCGCATCGGACACCACCAGCTCAGAAATCGCCTGATCGCAGCGCCCATGGCTGGCATTACTGACAGACCATTCCGGAC
GCTGTGCTACGAGATGGGAGCGGGTTTAACCGTTTCCGAGATGATGTCTTCTAACCCACAGGTGTGGGAGAGCGACAAGT
CCCGTTTACGGATGGTGCATATCGATGAGCCCGGTATTCGTACCGTGCAAATCGCCGGTAGCGTGCCGGAAGAGATGGCT
GCCGCCGCGCGGATTAACGTGGAAAGCGGCGCCCAGATTATTGATATCAATATGGGATGCCCGGCGAAAAAGGTGAATCG
CAAGCTTGCAGGTTCAGCCCTCTTGCAGTACCCCGACCAGGTGAAGTCGATCCTGACGGCGGTCGTTAACGCGGTGGACG
TTCCTGTGACTCTCAAGATTCGCACCGGTTGGGAACCGGAACACCGTAACTGCGTAGAGATTGCCCAACTGGCCGAAGAG
TGTGGCATTCAGGCTCTGACTATTCACGGACGCACCCGCGCCTGCTTGTTTAACGGAGACGCTGAGTACGACAGTATTCG
GGCAGTTAAGCAGAAAGTTTCCATTCCGATTATCGCGAATGGCGACATTACTGACCCGCTTAAAGCCAGAGCTGTGCTCG
ACTATACAGGGGCTGATGCCCTGATGATAGGCCGTGCAGCTCAGGGAAGACCCTGGATCTTTCGGGAAATCCAGCATTAT
CTGGACACTGGAGAGCTGCTGCCCCCGCTGCCGCTGGCAGAGGTGAAGCGCTTGCTTTGTGCGCACGTTCGGGAGTTGCA
TGACTTTTATGGTCAGGCAAAAGGGTACCGAATCGCGCGTAAACACGTCTCCTGGTATCTTCAGGAACATGCTCCAGATG
ACCAGTTTCGGCGCACATTCAACGCCATTGAGGATGCCAGCGAACAGCTGGAGGCGTTGGAGGCATACTTCGAAAATTTT
GCGTAA

Upstream 100 bases:

>100_bases
ATCTGCAACAGAGGATTGCTCAAAGTTTGGCCTTTCATCTCGTGCAAAAAATGCGTAATATACGCCGCCTTGCAGTCACA
GTATGGTCATTTCTTAACTC

Downstream 100 bases:

>100_bases
TAGAAATAAAGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGTACTGACCGTTTCTACCGTTAACTCTCA
GGATCAGGTAACTCAAAAGC

Product: tRNA-dihydrouridine synthase B

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 321; Mature: 321

Protein sequence:

>321_residues
MRIGHHQLRNRLIAAPMAGITDRPFRTLCYEMGAGLTVSEMMSSNPQVWESDKSRLRMVHIDEPGIRTVQIAGSVPEEMA
AAARINVESGAQIIDINMGCPAKKVNRKLAGSALLQYPDQVKSILTAVVNAVDVPVTLKIRTGWEPEHRNCVEIAQLAEE
CGIQALTIHGRTRACLFNGDAEYDSIRAVKQKVSIPIIANGDITDPLKARAVLDYTGADALMIGRAAQGRPWIFREIQHY
LDTGELLPPLPLAEVKRLLCAHVRELHDFYGQAKGYRIARKHVSWYLQEHAPDDQFRRTFNAIEDASEQLEALEAYFENF
A

Sequences:

>Translated_321_residues
MRIGHHQLRNRLIAAPMAGITDRPFRTLCYEMGAGLTVSEMMSSNPQVWESDKSRLRMVHIDEPGIRTVQIAGSVPEEMA
AAARINVESGAQIIDINMGCPAKKVNRKLAGSALLQYPDQVKSILTAVVNAVDVPVTLKIRTGWEPEHRNCVEIAQLAEE
CGIQALTIHGRTRACLFNGDAEYDSIRAVKQKVSIPIIANGDITDPLKARAVLDYTGADALMIGRAAQGRPWIFREIQHY
LDTGELLPPLPLAEVKRLLCAHVRELHDFYGQAKGYRIARKHVSWYLQEHAPDDQFRRTFNAIEDASEQLEALEAYFENF
A
>Mature_321_residues
MRIGHHQLRNRLIAAPMAGITDRPFRTLCYEMGAGLTVSEMMSSNPQVWESDKSRLRMVHIDEPGIRTVQIAGSVPEEMA
AAARINVESGAQIIDINMGCPAKKVNRKLAGSALLQYPDQVKSILTAVVNAVDVPVTLKIRTGWEPEHRNCVEIAQLAEE
CGIQALTIHGRTRACLFNGDAEYDSIRAVKQKVSIPIIANGDITDPLKARAVLDYTGADALMIGRAAQGRPWIFREIQHY
LDTGELLPPLPLAEVKRLLCAHVRELHDFYGQAKGYRIARKHVSWYLQEHAPDDQFRRTFNAIEDASEQLEALEAYFENF
A

Specific function: Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs [H]

COG id: COG0042

COG function: function code J; tRNA-dihydrouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dus family. DusB subfamily [H]

Homologues:

Organism=Homo sapiens, GI239788483, Length=239, Percent_Identity=31.3807531380753, Blast_Score=109, Evalue=3e-24,
Organism=Homo sapiens, GI239788462, Length=241, Percent_Identity=31.9502074688797, Blast_Score=107, Evalue=1e-23,
Organism=Homo sapiens, GI8923374, Length=245, Percent_Identity=31.0204081632653, Blast_Score=102, Evalue=4e-22,
Organism=Homo sapiens, GI31742496, Length=320, Percent_Identity=29.6875, Blast_Score=100, Evalue=2e-21,
Organism=Homo sapiens, GI40807366, Length=228, Percent_Identity=30.2631578947368, Blast_Score=89, Evalue=4e-18,
Organism=Escherichia coli, GI1789660, Length=321, Percent_Identity=95.6386292834891, Blast_Score=637, Evalue=0.0,
Organism=Escherichia coli, GI1788462, Length=324, Percent_Identity=28.7037037037037, Blast_Score=114, Evalue=8e-27,
Organism=Caenorhabditis elegans, GI17543114, Length=241, Percent_Identity=31.5352697095436, Blast_Score=97, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI17510279, Length=247, Percent_Identity=33.6032388663968, Blast_Score=97, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI17507177, Length=252, Percent_Identity=30.1587301587302, Blast_Score=92, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI25144369, Length=224, Percent_Identity=32.5892857142857, Blast_Score=84, Evalue=1e-16,
Organism=Saccharomyces cerevisiae, GI6323433, Length=267, Percent_Identity=31.0861423220974, Blast_Score=97, Evalue=3e-21,
Organism=Saccharomyces cerevisiae, GI6323560, Length=231, Percent_Identity=28.1385281385281, Blast_Score=87, Evalue=4e-18,
Organism=Saccharomyces cerevisiae, GI6323437, Length=227, Percent_Identity=28.1938325991189, Blast_Score=77, Evalue=3e-15,
Organism=Drosophila melanogaster, GI24585320, Length=244, Percent_Identity=31.5573770491803, Blast_Score=112, Evalue=5e-25,
Organism=Drosophila melanogaster, GI45549423, Length=248, Percent_Identity=33.4677419354839, Blast_Score=100, Evalue=2e-21,
Organism=Drosophila melanogaster, GI24580595, Length=233, Percent_Identity=30.9012875536481, Blast_Score=97, Evalue=1e-20,
Organism=Drosophila melanogaster, GI19920448, Length=233, Percent_Identity=30.9012875536481, Blast_Score=97, Evalue=1e-20,
Organism=Drosophila melanogaster, GI19921524, Length=229, Percent_Identity=28.82096069869, Blast_Score=84, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR004652
- InterPro:   IPR001269
- InterPro:   IPR018517 [H]

Pfam domain/function: PF01207 Dus [H]

EC number: NA

Molecular weight: Translated: 35836; Mature: 35836

Theoretical pI: Translated: 6.85; Mature: 6.85

Prosite motif: PS01136 UPF0034

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIGHHQLRNRLIAAPMAGITDRPFRTLCYEMGAGLTVSEMMSSNPQVWESDKSRLRMVH
CCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCHHHHHCCCCCCCCCCCCCEEEEE
IDEPGIRTVQIAGSVPEEMAAAARINVESGAQIIDINMGCPAKKVNRKLAGSALLQYPDQ
ECCCCCEEEEECCCCCHHHHHHHHEECCCCCEEEEECCCCCHHHHHHHHHHHHHHHCHHH
VKSILTAVVNAVDVPVTLKIRTGWEPEHRNCVEIAQLAEECGIQALTIHGRTRACLFNGD
HHHHHHHHHHHHCCCEEEEEECCCCCCCCCHHHHHHHHHHCCCEEEEECCCCEEEEECCC
AEYDSIRAVKQKVSIPIIANGDITDPLKARAVLDYTGADALMIGRAAQGRPWIFREIQHY
CCHHHHHHHHHHCCCEEEECCCCCCCHHHHEEEEECCCCEEEECCCCCCCCHHHHHHHHH
LDTGELLPPLPLAEVKRLLCAHVRELHDFYGQAKGYRIARKHVSWYLQEHAPDDQFRRTF
HCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCHHHHHHH
NAIEDASEQLEALEAYFENFA
HHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MRIGHHQLRNRLIAAPMAGITDRPFRTLCYEMGAGLTVSEMMSSNPQVWESDKSRLRMVH
CCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCHHHHHCCCCCCCCCCCCCEEEEE
IDEPGIRTVQIAGSVPEEMAAAARINVESGAQIIDINMGCPAKKVNRKLAGSALLQYPDQ
ECCCCCEEEEECCCCCHHHHHHHHEECCCCCEEEEECCCCCHHHHHHHHHHHHHHHCHHH
VKSILTAVVNAVDVPVTLKIRTGWEPEHRNCVEIAQLAEECGIQALTIHGRTRACLFNGD
HHHHHHHHHHHHCCCEEEEEECCCCCCCCCHHHHHHHHHHCCCEEEEECCCCEEEEECCC
AEYDSIRAVKQKVSIPIIANGDITDPLKARAVLDYTGADALMIGRAAQGRPWIFREIQHY
CCHHHHHHHHHHCCCEEEECCCCCCCHHHHEEEEECCCCEEEECCCCCCCCHHHHHHHHH
LDTGELLPPLPLAEVKRLLCAHVRELHDFYGQAKGYRIARKHVSWYLQEHAPDDQFRRTF
HCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCHHHHHHH
NAIEDASEQLEALEAYFENFA
HHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]