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The map label for this gene is ispB [H]

Identifier: 238896694

GI number: 238896694

Start: 4673702

End: 4674673

Strand: Direct

Name: ispB [H]

Synonym: KP1_4908

Alternate gene names: 238896694

Gene position: 4673702-4674673 (Clockwise)

Preceding gene: 238896689

Following gene: 238896695

Centisome position: 89.05

GC content: 56.48

Gene sequence:

>972_bases
ATGAATTTAGAAAAAATCAATGAGTTAACCGCGCAAGATATGGCGGGTGTCAATGCGACGATTCTTGAGCAACTCAACTC
CGATGTTCAGCTTATCAATCAGTTAGGCTATTACATCGTCAGCGGCGGCGGCAAACGCATTCGCCCGATGATTGCTGTGC
TCGCCGCACGCGCGGTGGGCTATCAGGGGAGCGCGCATGTCACCATTGCCGCGCTGATTGAATTTATTCATACCGCCACC
CTGCTGCATGACGACGTCGTCGATGAGTCTGATATGCGTCGCGGCAAAGCCACGGCCAATGCGGCGTTTGGCAATGCCGC
CAGCGTGCTGGTCGGCGATTTTATCTATACCCGCGCCTTTCAGATGATGACCCAGCTGGGCTCGCTGAAGATCCTTGAGG
TCATGTCGGAAGCAGTGAACGTCATTGCCGAAGGCGAAGTGCTGCAGCTGATGAACGTCAATGACCCGGATATCACCGAA
GAGAACTACATGCGCGTTATCTACAGCAAGACAGCGCGCCTGTTTGAAGCTGCCTCGCAGTGTGCGGGACTGCTGGCCGA
CTGCACTGCGGAAGAAGAGCGCGCGTTGCAGGATTATGGCCGCTATCTCGGCACCGCTTTCCAGCTAATCGACGATTTAC
TCGATTATAGCTCTGACGGCGAGCGGCTCGGTAAAAACGTCGGTGACGACCTGAACGAAGGTAAACCCACGCTACCGCTG
CTGCACGCCATGCACCATGGCACACCCGATCAATCAGCGATGATCCGCGGTGCGATCGAGCAAGGCAACGGCCGCCATCT
ACTGGATGCTGTGCTGGAAACGATGGCGACCTGCGGTTCGCTGGAATGGACGCAGAAGCGTGCCGAAGAAGAAGCTGACA
AAGCCATTGCCGCGCTGCAGATCCTCCCGGACACGCCCTGGCGCGAAGCCTTGATTGGGCTGGCGCATATTGCAGTCCAG
CGCGATCATTAA

Upstream 100 bases:

>100_bases
CAATTTTTCAGTACAATCATATCTTCTTTTAATTCTGTCTTAATCCTTCTGTGATCCCACCTGGATTCATGGTAACAGGA
CATTTGGCCCGGCTTTTGCG

Downstream 100 bases:

>100_bases
TCCCCCCTCCTTTCCCGCGCGACGCGCGGGAAAGTTCCAGTAAAATGCCATTTCTCTCCCTAAACTTCCGAATATCTTGA
TAGATTCTGAATATTCCGGC

Product: octaprenyl-diphosphate synthase

Products: NA

Alternate protein names: All-trans-octaprenyl-diphosphate synthase; Octaprenyl pyrophosphate synthase; OPP synthase [H]

Number of amino acids: Translated: 323; Mature: 323

Protein sequence:

>323_residues
MNLEKINELTAQDMAGVNATILEQLNSDVQLINQLGYYIVSGGGKRIRPMIAVLAARAVGYQGSAHVTIAALIEFIHTAT
LLHDDVVDESDMRRGKATANAAFGNAASVLVGDFIYTRAFQMMTQLGSLKILEVMSEAVNVIAEGEVLQLMNVNDPDITE
ENYMRVIYSKTARLFEAASQCAGLLADCTAEEERALQDYGRYLGTAFQLIDDLLDYSSDGERLGKNVGDDLNEGKPTLPL
LHAMHHGTPDQSAMIRGAIEQGNGRHLLDAVLETMATCGSLEWTQKRAEEEADKAIAALQILPDTPWREALIGLAHIAVQ
RDH

Sequences:

>Translated_323_residues
MNLEKINELTAQDMAGVNATILEQLNSDVQLINQLGYYIVSGGGKRIRPMIAVLAARAVGYQGSAHVTIAALIEFIHTAT
LLHDDVVDESDMRRGKATANAAFGNAASVLVGDFIYTRAFQMMTQLGSLKILEVMSEAVNVIAEGEVLQLMNVNDPDITE
ENYMRVIYSKTARLFEAASQCAGLLADCTAEEERALQDYGRYLGTAFQLIDDLLDYSSDGERLGKNVGDDLNEGKPTLPL
LHAMHHGTPDQSAMIRGAIEQGNGRHLLDAVLETMATCGSLEWTQKRAEEEADKAIAALQILPDTPWREALIGLAHIAVQ
RDH
>Mature_323_residues
MNLEKINELTAQDMAGVNATILEQLNSDVQLINQLGYYIVSGGGKRIRPMIAVLAARAVGYQGSAHVTIAALIEFIHTAT
LLHDDVVDESDMRRGKATANAAFGNAASVLVGDFIYTRAFQMMTQLGSLKILEVMSEAVNVIAEGEVLQLMNVNDPDITE
ENYMRVIYSKTARLFEAASQCAGLLADCTAEEERALQDYGRYLGTAFQLIDDLLDYSSDGERLGKNVGDDLNEGKPTLPL
LHAMHHGTPDQSAMIRGAIEQGNGRHLLDAVLETMATCGSLEWTQKRAEEEADKAIAALQILPDTPWREALIGLAHIAVQ
RDH

Specific function: Supplies octaprenyl diphosphate, the precursor for the side chain of the isoprenoid quinones ubiquinone and menaquinone [H]

COG id: COG0142

COG function: function code H; Geranylgeranyl pyrophosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FPP/GGPP synthase family [H]

Homologues:

Organism=Homo sapiens, GI50659086, Length=323, Percent_Identity=30.9597523219814, Blast_Score=150, Evalue=1e-36,
Organism=Homo sapiens, GI4758430, Length=268, Percent_Identity=24.2537313432836, Blast_Score=73, Evalue=3e-13,
Organism=Homo sapiens, GI83700220, Length=268, Percent_Identity=24.2537313432836, Blast_Score=73, Evalue=3e-13,
Organism=Escherichia coli, GI1789578, Length=322, Percent_Identity=91.6149068322981, Blast_Score=614, Evalue=1e-177,
Organism=Escherichia coli, GI1786623, Length=250, Percent_Identity=29.2, Blast_Score=75, Evalue=7e-15,
Organism=Caenorhabditis elegans, GI17505681, Length=343, Percent_Identity=31.4868804664723, Blast_Score=147, Evalue=6e-36,
Organism=Saccharomyces cerevisiae, GI6319475, Length=298, Percent_Identity=33.2214765100671, Blast_Score=140, Evalue=4e-34,
Organism=Saccharomyces cerevisiae, GI6325188, Length=241, Percent_Identity=24.4813278008299, Blast_Score=85, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24651612, Length=298, Percent_Identity=30.5369127516779, Blast_Score=145, Evalue=3e-35,
Organism=Drosophila melanogaster, GI281365769, Length=284, Percent_Identity=23.5915492957746, Blast_Score=74, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24660002, Length=284, Percent_Identity=23.5915492957746, Blast_Score=74, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000092
- InterPro:   IPR017446
- InterPro:   IPR008949 [H]

Pfam domain/function: PF00348 polyprenyl_synt [H]

EC number: =2.5.1.90 [H]

Molecular weight: Translated: 35210; Mature: 35210

Theoretical pI: Translated: 4.46; Mature: 4.46

Prosite motif: PS00723 POLYPRENYL_SYNTHET_1 ; PS00444 POLYPRENYL_SYNTHET_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLEKINELTAQDMAGVNATILEQLNSDVQLINQLGYYIVSGGGKRIRPMIAVLAARAVG
CCHHHHHHHHHHHHCCCHHHHHHHHCHHHHHHHHHCCEEECCCCCHHHHHHHHHHHHHHC
YQGSAHVTIAALIEFIHTATLLHDDVVDESDMRRGKATANAAFGNAASVLVGDFIYTRAF
CCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCHHHHHHCCCHHHHHHHHHHHHHHH
QMMTQLGSLKILEVMSEAVNVIAEGEVLQLMNVNDPDITEENYMRVIYSKTARLFEAASQ
HHHHHHCCHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHH
CAGLLADCTAEEERALQDYGRYLGTAFQLIDDLLDYSSDGERLGKNVGDDLNEGKPTLPL
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCCCCCCCHHH
LHAMHHGTPDQSAMIRGAIEQGNGRHLLDAVLETMATCGSLEWTQKRAEEEADKAIAALQ
HHHHHCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
ILPDTPWREALIGLAHIAVQRDH
HCCCCCHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MNLEKINELTAQDMAGVNATILEQLNSDVQLINQLGYYIVSGGGKRIRPMIAVLAARAVG
CCHHHHHHHHHHHHCCCHHHHHHHHCHHHHHHHHHCCEEECCCCCHHHHHHHHHHHHHHC
YQGSAHVTIAALIEFIHTATLLHDDVVDESDMRRGKATANAAFGNAASVLVGDFIYTRAF
CCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCHHHHHHCCCHHHHHHHHHHHHHHH
QMMTQLGSLKILEVMSEAVNVIAEGEVLQLMNVNDPDITEENYMRVIYSKTARLFEAASQ
HHHHHHCCHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHH
CAGLLADCTAEEERALQDYGRYLGTAFQLIDDLLDYSSDGERLGKNVGDDLNEGKPTLPL
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCCCCCCCHHH
LHAMHHGTPDQSAMIRGAIEQGNGRHLLDAVLETMATCGSLEWTQKRAEEEADKAIAALQ
HHHHHCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
ILPDTPWREALIGLAHIAVQRDH
HCCCCCHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8312607; 9278503; 2670911; 8037730 [H]