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The map label for this gene is rpoD [H]

Identifier: 238896571

GI number: 238896571

Start: 4549610

End: 4551508

Strand: Direct

Name: rpoD [H]

Synonym: KP1_4766

Alternate gene names: 238896571

Gene position: 4549610-4551508 (Clockwise)

Preceding gene: 238896570

Following gene: 238896573

Centisome position: 86.68

GC content: 55.45

Gene sequence:

>1899_bases
ATGTCGACAGCCCGTCTGCCGACACCAATCAAACGAATTAAGTGTGGATACCGTCTTATGGAGCAAAACCCGCAGTCACA
GCTGAAGCTTCTTGTCCAACGTGGTAAGGAGCAAGGCTATCTGACCTATGCCGAGGTCAATGACCATCTGCCGGAAGATA
TCGTCGATTCCGATCAGATCGAAGACATCATCCAAATGATCAACGACATGGGTATTCAGGTGATGGAAGAAGCACCGGAT
GCCGATGATCTGATGCTTGCCGAAAACACCGCGGATGAAGATGCTGCCGAAGCCGCCGCGCAGGTGCTCTCCAGCGTGGA
ATCCGAAATCGGGCGCACGACCGACCCGGTGCGTATGTACATGCGTGAAATGGGTACCGTAGAGCTACTGACCCGTGAAG
GCGAAATTGACATCGCCAAACGCATCGAAGACGGCATCAATCAGGTGCAGTGCTCCGTTGCCGAATATCCGGAAGCGATC
ACCTACCTGCTTGAGCAGTACGACCGCGTTGAAGCGGAAGAAGCCCGTCTGTCCGATCTGATCACCGGTTTCGTCGATCC
GAACGCCGAAGAAGATATGGCGCCAACCGCCACCCACGTTGGCTCCGAGCTGTCTCAGGAAGAGATGGATGACGACGAAG
ACGAAGACGAAGATGAAGACGCCGACGACAACAGCGATGACGACAACAGCATCGACCCAGAGCTGGCCCGTGAGAAATTC
GCCGAGCTGCGTACCCAGTACGAGCTGACGCGCGACACCATCAAAGCCAAAGGTCGTAGCCATGCCGCCGCCCAGGAAGA
GATCCTGAAGCTGTCTGAAGTCTTCAAGCAGTTCCGCCTGGTGCCGAAGCAGTTCGATTACCTGGTCAACAGCATGCGCA
GCATGATGGATCGCGTTCGTACTCAGGAACGTATCATCATGAAGCTGTGCGTTGAGCAGTGCAAAATGCCGAAGAAAAAC
TTCATCACCCTGTTCACCGGCAACGAAACCAGCGAGACCTGGTTCAACGCCGCGGTGGCAATGAACAAGCCGTGGTCTGA
GAAGCTGCTGGAAGTGAAAGAAGATGTGCAGCGCGGCCTGCAGAAGCTGCAGCAGATTGAAGAAGAGACCGGCCTGACCA
TCGAGCAGGTTAAAGATATCAACCGTCGCATGTCCATCGGCGAAGCGAAAGCCCGTCGTGCGAAGAAAGAGATGGTTGAA
GCGAACCTGCGTCTGGTTATCTCTATCGCCAAGAAATACACCAACCGCGGTCTGCAGTTCCTCGACCTGATTCAGGAAGG
CAACATCGGTCTGATGAAAGCGGTCGATAAGTTCGAATACCGTCGCGGCTACAAGTTCTCGACCTACGCCACCTGGTGGA
TCCGTCAGGCCATCACCCGCTCTATCGCGGATCAGGCGCGCACCATCCGTATTCCGGTGCATATGATTGAGACTATCAAC
AAGCTCAACCGTATCTCCCGTCAGATGCTGCAGGAGATGGGCCGTGAACCGACCCCGGAAGAGCTGGCTGAGCGTATGCT
GATGCCGGAAGACAAAATCCGTAAAGTGCTGAAGATCGCCAAAGAGCCTATCTCCATGGAAACGCCGATCGGCGACGATG
AAGATTCGCATCTGGGGGATTTCATCGAGGATACCACCCTCGAGCTGCCGCTGGATTCTGCAACCACCGAGAGCCTGCGC
GCGGCAACGCATGACGTGCTGGCCGGCCTCACCGCTCGTGAAGCGAAAGTGCTGCGTATGCGTTTCGGTATCGACATGAA
CACCGACCACACGCTGGAAGAAGTGGGTAAACAGTTCGACGTTACCCGCGAACGTATCCGTCAGATCGAAGCGAAGGCGC
TGCGTAAACTGCGCCACCCGAGCCGTTCTGAAGTGCTGCGTAGCTTCCTCGACGATTAA

Upstream 100 bases:

>100_bases
GAAAAGTATTTAACGGCTTAAGTGCCGAGTATCGATCGGGAAGCCCCCGACAGCCGCGCAGAGGCGCAGCGGCAATAATA
TAAGTACGCCCTCGCTTTAA

Downstream 100 bases:

>100_bases
TCGTCGCGGGGCATCGTCTACAGCCTGTTAAAAAGCTCCCTGCGGGGAGCTTTTTTTATGGCGTTCAGGTGGCCATCCCG
GGAAAGTCGCCTCTCTCCGC

Product: RNA polymerase sigma factor RpoD

Products: NA

Alternate protein names: Sigma-70 [H]

Number of amino acids: Translated: 632; Mature: 631

Protein sequence:

>632_residues
MSTARLPTPIKRIKCGYRLMEQNPQSQLKLLVQRGKEQGYLTYAEVNDHLPEDIVDSDQIEDIIQMINDMGIQVMEEAPD
ADDLMLAENTADEDAAEAAAQVLSSVESEIGRTTDPVRMYMREMGTVELLTREGEIDIAKRIEDGINQVQCSVAEYPEAI
TYLLEQYDRVEAEEARLSDLITGFVDPNAEEDMAPTATHVGSELSQEEMDDDEDEDEDEDADDNSDDDNSIDPELAREKF
AELRTQYELTRDTIKAKGRSHAAAQEEILKLSEVFKQFRLVPKQFDYLVNSMRSMMDRVRTQERIIMKLCVEQCKMPKKN
FITLFTGNETSETWFNAAVAMNKPWSEKLLEVKEDVQRGLQKLQQIEEETGLTIEQVKDINRRMSIGEAKARRAKKEMVE
ANLRLVISIAKKYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRSIADQARTIRIPVHMIETIN
KLNRISRQMLQEMGREPTPEELAERMLMPEDKIRKVLKIAKEPISMETPIGDDEDSHLGDFIEDTTLELPLDSATTESLR
AATHDVLAGLTAREAKVLRMRFGIDMNTDHTLEEVGKQFDVTRERIRQIEAKALRKLRHPSRSEVLRSFLDD

Sequences:

>Translated_632_residues
MSTARLPTPIKRIKCGYRLMEQNPQSQLKLLVQRGKEQGYLTYAEVNDHLPEDIVDSDQIEDIIQMINDMGIQVMEEAPD
ADDLMLAENTADEDAAEAAAQVLSSVESEIGRTTDPVRMYMREMGTVELLTREGEIDIAKRIEDGINQVQCSVAEYPEAI
TYLLEQYDRVEAEEARLSDLITGFVDPNAEEDMAPTATHVGSELSQEEMDDDEDEDEDEDADDNSDDDNSIDPELAREKF
AELRTQYELTRDTIKAKGRSHAAAQEEILKLSEVFKQFRLVPKQFDYLVNSMRSMMDRVRTQERIIMKLCVEQCKMPKKN
FITLFTGNETSETWFNAAVAMNKPWSEKLLEVKEDVQRGLQKLQQIEEETGLTIEQVKDINRRMSIGEAKARRAKKEMVE
ANLRLVISIAKKYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRSIADQARTIRIPVHMIETIN
KLNRISRQMLQEMGREPTPEELAERMLMPEDKIRKVLKIAKEPISMETPIGDDEDSHLGDFIEDTTLELPLDSATTESLR
AATHDVLAGLTAREAKVLRMRFGIDMNTDHTLEEVGKQFDVTRERIRQIEAKALRKLRHPSRSEVLRSFLDD
>Mature_631_residues
STARLPTPIKRIKCGYRLMEQNPQSQLKLLVQRGKEQGYLTYAEVNDHLPEDIVDSDQIEDIIQMINDMGIQVMEEAPDA
DDLMLAENTADEDAAEAAAQVLSSVESEIGRTTDPVRMYMREMGTVELLTREGEIDIAKRIEDGINQVQCSVAEYPEAIT
YLLEQYDRVEAEEARLSDLITGFVDPNAEEDMAPTATHVGSELSQEEMDDDEDEDEDEDADDNSDDDNSIDPELAREKFA
ELRTQYELTRDTIKAKGRSHAAAQEEILKLSEVFKQFRLVPKQFDYLVNSMRSMMDRVRTQERIIMKLCVEQCKMPKKNF
ITLFTGNETSETWFNAAVAMNKPWSEKLLEVKEDVQRGLQKLQQIEEETGLTIEQVKDINRRMSIGEAKARRAKKEMVEA
NLRLVISIAKKYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRSIADQARTIRIPVHMIETINK
LNRISRQMLQEMGREPTPEELAERMLMPEDKIRKVLKIAKEPISMETPIGDDEDSHLGDFIEDTTLELPLDSATTESLRA
ATHDVLAGLTAREAKVLRMRFGIDMNTDHTLEEVGKQFDVTRERIRQIEAKALRKLRHPSRSEVLRSFLDD

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This is the primary sigma factor of this bacterium [H]

COG id: COG0568

COG function: function code K; DNA-directed RNA polymerase, sigma subunit (sigma70/sigma32)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789448, Length=613, Percent_Identity=95.9216965742251, Blast_Score=1149, Evalue=0.0,
Organism=Escherichia coli, GI1789098, Length=247, Percent_Identity=42.1052631578947, Blast_Score=209, Evalue=5e-55,
Organism=Escherichia coli, GI1789871, Length=242, Percent_Identity=28.5123966942149, Blast_Score=89, Evalue=8e-19,
Organism=Escherichia coli, GI1788231, Length=202, Percent_Identity=28.7128712871287, Blast_Score=65, Evalue=1e-11,

Paralogues:

None

Copy number: 700 (log & stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR009042
- InterPro:   IPR007627
- InterPro:   IPR007624
- InterPro:   IPR007630
- InterPro:   IPR007631
- InterPro:   IPR007127
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR012760
- InterPro:   IPR011991 [H]

Pfam domain/function: PF04546 Sigma70_ner; PF03979 Sigma70_r1_1; PF00140 Sigma70_r1_2; PF04542 Sigma70_r2; PF04539 Sigma70_r3; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 72585; Mature: 72454

Theoretical pI: Translated: 4.49; Mature: 4.49

Prosite motif: PS00715 SIGMA70_1 ; PS00716 SIGMA70_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTARLPTPIKRIKCGYRLMEQNPQSQLKLLVQRGKEQGYLTYAEVNDHLPEDIVDSDQI
CCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCEEEECCCCCCCHHHCCHHHH
EDIIQMINDMGIQVMEEAPDADDLMLAENTADEDAAEAAAQVLSSVESEIGRTTDPVRMY
HHHHHHHHHHCHHHHHCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHH
MREMGTVELLTREGEIDIAKRIEDGINQVQCSVAEYPEAITYLLEQYDRVEAEEARLSDL
HHHCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ITGFVDPNAEEDMAPTATHVGSELSQEEMDDDEDEDEDEDADDNSDDDNSIDPELAREKF
HHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHH
AELRTQYELTRDTIKAKGRSHAAAQEEILKLSEVFKQFRLVPKQFDYLVNSMRSMMDRVR
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHH
TQERIIMKLCVEQCKMPKKNFITLFTGNETSETWFNAAVAMNKPWSEKLLEVKEDVQRGL
HHHHHHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
QKLQQIEEETGLTIEQVKDINRRMSIGEAKARRAKKEMVEANLRLVISIAKKYTNRGLQF
HHHHHHHHHHCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHH
LDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRSIADQARTIRIPVHMIETIN
HHHHHCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHEEEHHHHHHHHHH
KLNRISRQMLQEMGREPTPEELAERMLMPEDKIRKVLKIAKEPISMETPIGDDEDSHLGD
HHHHHHHHHHHHHCCCCCHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHH
FIEDTTLELPLDSATTESLRAATHDVLAGLTAREAKVLRMRFGIDMNTDHTLEEVGKQFD
HHHCCCEECCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHH
VTRERIRQIEAKALRKLRHPSRSEVLRSFLDD
HHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCC
>Mature Secondary Structure 
STARLPTPIKRIKCGYRLMEQNPQSQLKLLVQRGKEQGYLTYAEVNDHLPEDIVDSDQI
CCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCEEEECCCCCCCHHHCCHHHH
EDIIQMINDMGIQVMEEAPDADDLMLAENTADEDAAEAAAQVLSSVESEIGRTTDPVRMY
HHHHHHHHHHCHHHHHCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHH
MREMGTVELLTREGEIDIAKRIEDGINQVQCSVAEYPEAITYLLEQYDRVEAEEARLSDL
HHHCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ITGFVDPNAEEDMAPTATHVGSELSQEEMDDDEDEDEDEDADDNSDDDNSIDPELAREKF
HHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHH
AELRTQYELTRDTIKAKGRSHAAAQEEILKLSEVFKQFRLVPKQFDYLVNSMRSMMDRVR
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHH
TQERIIMKLCVEQCKMPKKNFITLFTGNETSETWFNAAVAMNKPWSEKLLEVKEDVQRGL
HHHHHHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
QKLQQIEEETGLTIEQVKDINRRMSIGEAKARRAKKEMVEANLRLVISIAKKYTNRGLQF
HHHHHHHHHHCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHH
LDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRSIADQARTIRIPVHMIETIN
HHHHHCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHEEEHHHHHHHHHH
KLNRISRQMLQEMGREPTPEELAERMLMPEDKIRKVLKIAKEPISMETPIGDDEDSHLGD
HHHHHHHHHHHHHCCCCCHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHH
FIEDTTLELPLDSATTESLRAATHDVLAGLTAREAKVLRMRFGIDMNTDHTLEEVGKQFD
HHHCCCEECCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHH
VTRERIRQIEAKALRKLRHPSRSEVLRSFLDD
HHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11677608; 12644504 [H]