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The map label for this gene is cca [H]

Identifier: 238896556

GI number: 238896556

Start: 4535590

End: 4536831

Strand: Direct

Name: cca [H]

Synonym: KP1_4747

Alternate gene names: 238896556

Gene position: 4535590-4536831 (Clockwise)

Preceding gene: 238896555

Following gene: 238896559

Centisome position: 86.42

GC content: 64.41

Gene sequence:

>1242_bases
GTGAAGAGTTATCTGGTCGGTGGCGCGGTGCGTGACGCGCTGTTAGGGCTGCCGGTCAAAGACCGAGACTGGGTGGTGGT
GGGCGCCACCCCGCAACAGATGCTAGACGCGGGCTATCAACAGGTAGGCCGCGATTTTCCCGTGTTCCTCCATCCGCAAA
GCCGGGAAGAGTATGCCCTGGCCCGCACCGAGCGCAAATCCGGCGCGGGCTATACCGGTTTTACCTGCTATGCCGCCCCG
GACGTCACCCTCGAAGCCGATCTGCTGCGCCGCGACCTGACGGTCAATGCCCTCGCCCAGGATGCCGACGGCGCAATTAT
CGACCCCTACGGCGGACAGAACGATCTCCGTCAGCGCCTGCTGCGCCATGTCTCTCCCGCGTTTAGTGAAGATCCGCTGC
GCGTGCTGCGCGTCGCGCGTTTTGCCGCCCGCTACGCCCACCTCGGTTTTCGCATTGCCGAAGAGACCCAGGCCCTGATG
GCCGCGATGGTTGAGGCCGGCGAGCTGGCGCATTTAACGCCGGAGCGGGTGTGGAAGGAGACGGAAAGCGCATTAACTAC
TCGCAACCCGCAGGTCTTTTTCCAGACGCTGCGCGACTGTCAGGCTCTCAAGGTGCTGTTCCCGGAGATTGACGCCCTGT
ACGGCGTGCCGGCCCCGGCGAAATGGCATCCGGAAATCGACACCGGCCTGCACACCCTGATGACCGTCACGATGGCCGCG
ATGCTGTCGCCGGATGTCGACGTACGTTTCGCCACCCTGTGCCATGACCTCGGCAAAGGGCTGACGCCGAAGGCGCTCTG
GCCGCGTCACCACGGCCATGGTCCGGCGGGAGTCAAGCTGGTGGAGCAGCTCTGCGCGCGTCTGCGGGTGCCAAACGATA
TCCGCGATCTCGCGAAACTGGTGGCGGAGTACCATGACCTAATCCACACCCTGCCAATTCTGCAGCCGAAAACCCTCGTC
AAACTCTTCGACAGCATTGACGCGTGGCGCAAACCGCAGCGCGTGCAGCAGATCGCGCTCACCAGCGAAGCCGACGTCCG
CGGACGCACCGGTTTCGAGGCCAGCGATTACCCGCAGGGGCGCCTGCTGCTGGAGGCCTGGGAAGTGGCGCAGTCGGTCT
CCACTAAAGAGGTCGTGGCGGCGGGCTTTAAAGGGGCGGAAATTCGCGAGGAGCTGACGCGGCGGAGAATTGCGGCGGTA
GCGCAGTGGAAGGAACAGCGTTGCCCCCAGCCGCAAGGCTGA

Upstream 100 bases:

>100_bases
GTGCCAAGCCGCAAACGTAAAGACCGGTGGATGAACTAATTCGTCTTCTCTGCCAGACTTACGTATCATCTTGCGAAAAA
CGGATACGGGAGAATCAGGC

Downstream 100 bases:

>100_bases
GGGCGCAGGCCTTAGAAGAAGACCACGTACACCGCTGCCGCGACGATAAAGCGGTAGATAGCGAACGGAATGAACGAGAT
CCGTTTGATCAGCTGCAGGA

Product: multifunctional tRNA nucleotidyl transferase/2'3'-cyclic phosphodiesterase/2'nucleotidase/phosphatase

Products: NA

Alternate protein names: CCA-adding enzyme; tRNA CCA-pyrophosphorylase; tRNA adenylyl-/cytidylyl-transferase; tRNA nucleotidyltransferase; tRNA-NT; 2'-nucleotidase; 2',3'-cyclic phosphodiesterase; Phosphatase [H]

Number of amino acids: Translated: 413; Mature: 413

Protein sequence:

>413_residues
MKSYLVGGAVRDALLGLPVKDRDWVVVGATPQQMLDAGYQQVGRDFPVFLHPQSREEYALARTERKSGAGYTGFTCYAAP
DVTLEADLLRRDLTVNALAQDADGAIIDPYGGQNDLRQRLLRHVSPAFSEDPLRVLRVARFAARYAHLGFRIAEETQALM
AAMVEAGELAHLTPERVWKETESALTTRNPQVFFQTLRDCQALKVLFPEIDALYGVPAPAKWHPEIDTGLHTLMTVTMAA
MLSPDVDVRFATLCHDLGKGLTPKALWPRHHGHGPAGVKLVEQLCARLRVPNDIRDLAKLVAEYHDLIHTLPILQPKTLV
KLFDSIDAWRKPQRVQQIALTSEADVRGRTGFEASDYPQGRLLLEAWEVAQSVSTKEVVAAGFKGAEIREELTRRRIAAV
AQWKEQRCPQPQG

Sequences:

>Translated_413_residues
MKSYLVGGAVRDALLGLPVKDRDWVVVGATPQQMLDAGYQQVGRDFPVFLHPQSREEYALARTERKSGAGYTGFTCYAAP
DVTLEADLLRRDLTVNALAQDADGAIIDPYGGQNDLRQRLLRHVSPAFSEDPLRVLRVARFAARYAHLGFRIAEETQALM
AAMVEAGELAHLTPERVWKETESALTTRNPQVFFQTLRDCQALKVLFPEIDALYGVPAPAKWHPEIDTGLHTLMTVTMAA
MLSPDVDVRFATLCHDLGKGLTPKALWPRHHGHGPAGVKLVEQLCARLRVPNDIRDLAKLVAEYHDLIHTLPILQPKTLV
KLFDSIDAWRKPQRVQQIALTSEADVRGRTGFEASDYPQGRLLLEAWEVAQSVSTKEVVAAGFKGAEIREELTRRRIAAV
AQWKEQRCPQPQG
>Mature_413_residues
MKSYLVGGAVRDALLGLPVKDRDWVVVGATPQQMLDAGYQQVGRDFPVFLHPQSREEYALARTERKSGAGYTGFTCYAAP
DVTLEADLLRRDLTVNALAQDADGAIIDPYGGQNDLRQRLLRHVSPAFSEDPLRVLRVARFAARYAHLGFRIAEETQALM
AAMVEAGELAHLTPERVWKETESALTTRNPQVFFQTLRDCQALKVLFPEIDALYGVPAPAKWHPEIDTGLHTLMTVTMAA
MLSPDVDVRFATLCHDLGKGLTPKALWPRHHGHGPAGVKLVEQLCARLRVPNDIRDLAKLVAEYHDLIHTLPILQPKTLV
KLFDSIDAWRKPQRVQQIALTSEADVRGRTGFEASDYPQGRLLLEAWEVAQSVSTKEVVAAGFKGAEIREELTRRRIAAV
AQWKEQRCPQPQG

Specific function: Catalyzes the addition and repair of the essential 3'- terminal CCA sequence in tRNAs without using a nucleic acid template. Adds these three nucleotides in the order of C, C, and A to the tRNA nucleotide-73, using CTP and ATP as substrates and producing

COG id: COG0617

COG function: function code J; tRNA nucleotidyltransferase/poly(A) polymerase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family. Bacterial CCA-adding enzyme type 1 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789436, Length=412, Percent_Identity=86.4077669902913, Blast_Score=741, Evalue=0.0,
Organism=Escherichia coli, GI87081691, Length=321, Percent_Identity=27.1028037383178, Blast_Score=76, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI71995920, Length=259, Percent_Identity=27.027027027027, Blast_Score=69, Evalue=5e-12,
Organism=Drosophila melanogaster, GI281360112, Length=224, Percent_Identity=31.25, Blast_Score=75, Evalue=1e-13,
Organism=Drosophila melanogaster, GI21357337, Length=224, Percent_Identity=31.25, Blast_Score=75, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012006
- InterPro:   IPR003607
- InterPro:   IPR002646 [H]

Pfam domain/function: PF01743 PolyA_pol [H]

EC number: =2.7.7.21; =2.7.7.25 [H]

Molecular weight: Translated: 45986; Mature: 45986

Theoretical pI: Translated: 7.38; Mature: 7.38

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSYLVGGAVRDALLGLPVKDRDWVVVGATPQQMLDAGYQQVGRDFPVFLHPQSREEYAL
CCCCCCHHHHHHHHHCCCCCCCCEEEECCCHHHHHHHHHHHHCCCCCEEECCCCCHHHHH
ARTERKSGAGYTGFTCYAAPDVTLEADLLRRDLTVNALAQDADGAIIDPYGGQNDLRQRL
HHHHHHCCCCCCCEEEEECCCCEEHHHHHHHHHHHHHHHHCCCCCEECCCCCHHHHHHHH
LRHVSPAFSEDPLRVLRVARFAARYAHLGFRIAEETQALMAAMVEAGELAHLTPERVWKE
HHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHCCCHHHHHHH
TESALTTRNPQVFFQTLRDCQALKVLFPEIDALYGVPAPAKWHPEIDTGLHTLMTVTMAA
HHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHH
MLSPDVDVRFATLCHDLGKGLTPKALWPRHHGHGPAGVKLVEQLCARLRVPNDIRDLAKL
HHCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHH
VAEYHDLIHTLPILQPKTLVKLFDSIDAWRKPQRVQQIALTSEADVRGRTGFEASDYPQG
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCC
RLLLEAWEVAQSVSTKEVVAAGFKGAEIREELTRRRIAAVAQWKEQRCPQPQG
HHHHHHHHHHHHCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MKSYLVGGAVRDALLGLPVKDRDWVVVGATPQQMLDAGYQQVGRDFPVFLHPQSREEYAL
CCCCCCHHHHHHHHHCCCCCCCCEEEECCCHHHHHHHHHHHHCCCCCEEECCCCCHHHHH
ARTERKSGAGYTGFTCYAAPDVTLEADLLRRDLTVNALAQDADGAIIDPYGGQNDLRQRL
HHHHHHCCCCCCCEEEEECCCCEEHHHHHHHHHHHHHHHHCCCCCEECCCCCHHHHHHHH
LRHVSPAFSEDPLRVLRVARFAARYAHLGFRIAEETQALMAAMVEAGELAHLTPERVWKE
HHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHCCCHHHHHHH
TESALTTRNPQVFFQTLRDCQALKVLFPEIDALYGVPAPAKWHPEIDTGLHTLMTVTMAA
HHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHH
MLSPDVDVRFATLCHDLGKGLTPKALWPRHHGHGPAGVKLVEQLCARLRVPNDIRDLAKL
HHCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHH
VAEYHDLIHTLPILQPKTLVKLFDSIDAWRKPQRVQQIALTSEADVRGRTGFEASDYPQG
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCC
RLLLEAWEVAQSVSTKEVVAAGFKGAEIREELTRRRIAAVAQWKEQRCPQPQG
HHHHHHHHHHHHCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA