Click here to switch to the map view.

The map label for this gene is ptsP [H]

Identifier: 238896344

GI number: 238896344

Start: 4324785

End: 4327031

Strand: Reverse

Name: ptsP [H]

Synonym: KP1_4508

Alternate gene names: 238896344

Gene position: 4327031-4324785 (Counterclockwise)

Preceding gene: 238896345

Following gene: 238896343

Centisome position: 82.44

GC content: 62.66

Gene sequence:

>2247_bases
ATGCTCACTCGGTTGCGAGAAATAGTCGAGAAGGTCGCGAGCGCGCCGCGTTTAAACGAGGCGCTGGATATTCTGGTGAC
GGATGTCTGCCAGGCGATGGAGACGGAAGTCTGCTCGGTTTACCTGGCTGACAATGACCGACGCTGCTATTACCTGATGG
CCACCCGCGGGTTAAAAAAACCTCGCGGGCGCACCGTTGCGCTGGCGTTCGATGAAGGCCTGGTGGGGCTGGTTGGCCGC
CTGGCGGAACCCATCAACCTCGCCGATGCGCAAAAACACCCCAGCTTCAAATATATCCCCGCCGTCAAAGAGGACCGCTT
CCGCGCCTTCCTTGGCGTGCCGATCATTCAGCGCCGCCAGCTGCTGGGCGTGCTGGTGGTCCAGCAGCGCGAGCTGCGTC
AGTTTGATGAAAGCGAAGAGTCTTTCCTCGTCACCCTCGCCACCCAGATGGCGGCGATCCTCTCCCAGTCCCAGCTGAAC
GCCCTGTTCGGCCAGTATCGTCAAACGCGCATTCGCGCTCTGCCGGCCTCTTCCGGGGTGGCGATCGCCGAGGGCTGGAT
GGACGTCAGCCTGCCGCTGATGGAGCAGGTGTATGAAGCCTCGACGCTGGACACCGCCTCGGAACGCGAACGCCTGACCG
GTGCGCTGGAAGAGGCGGCCAACGAGTTTCGTCGCTACAGCAAACGCTACGCTGCCGGGGCGCAAAAAGAGACGGCGGCG
ATCTTCGATCTCTACTCGCACCTGCTCTCTGACGCCCGCCTGCGTCGCGAGCTGTTTGCCGAAGTTGACAAAGGCGCCGT
CGCCGAGTGGGCCGTCAAAAAGATCATTGAGAAGTTCGCCGAACAGTTTGCCGCGCTGAGCGACGGCTATTTGAAAGAGC
GGGCCGGCGATCTGCGTACCCTGGGCCAGCGTCTGCTGTTCCATCTCGACGACAGTATTCAGGGGCCCAACACCTGGCCG
GCGCGCATTATCCTCGTGGCGGACGAGCTGTCGGCCACCACCCTCGCCGAAGTGCCGCAGGATCGCCTGGCCGGCGTGGT
GGTGCGCGATGGCGCCGCCAACTCCCATGCCGCTATTATGGTGCGGGCCCTCGGCATCCCAACGGTGATGGGCGCCGATA
TTCAGCCCTCGCTGCTGCATGGCCATACGCTGATTGTCGATGGCTATCGCGGTGAACTGCTGGTGGATCCTGAACCTGTC
CTGTTGCAGGAGTATCAGCGCCTTATCAGCGAAGAGAACGAACTCAGCCGCCTGGCGGAGGACGATCTCCAGCGCGCGTC
GGAGCTGAAAAGCGGCGAGCGGGTGAAAGTCATGCTCAACGCCGGCTTAAGCCCGGAGCACGAAGAGAAGCTCGGCAGCT
TTGTTGACGGCATTGGCCTCTACCGCACCGAAATTCCCTTTATGCTGCAGAGCGGCTTCCCCTCAGAAGAGGAGCAGGTC
GCCCAGTATCAGGGCATGCTGCAGATGTTTAACAGCAAACCGGTGACCCTGCGTACCCTGGACATCGGCGCGGATAAACA
GCTGCCGTATATGCCGATCAGCGAAGAGAACCCGTGCCTCGGCTGGCGCGGGATCCGCATCACCCTCGATCAGCCGGAGA
TCTTTTTGATCCAGGTGCGGGCGATGCTGCGCGCCAACGCCGCCACCGGCAACCTCAGCATTTTGCTGCCGATGGTGACC
AGTCTGGAGGAGGTGGATGAGGCCCGTCGGCTGATCGATCGCGCCAGCCGGGAAGTGGAAGAGATGATCGGCTACGCCAT
CCCACGTCCGCGCCTGGGGGTGATGCTGGAGGTCCCGTCGATGGTCTTTATGCTGCCGCAGCTGGCCAGCCGGATCGATT
TTATCTCGGTAGGGACGAACGACCTGACCCAGTACCTGCTGGCCGTCGACCGCAACAATACCCGGGTCGCCAGCATGTAT
GACAGCCTGCATCCTGCGGTATTGCGTGCGCTGGCGATGATCGCCCATGACGCGGAGCGCTTTGGCATCGATCTGCGCCT
GTGCGGCGAGATGGCGGGCGACCCGATGTGCGTGACGATCCTTATCGGCCTCGGTTACCGCCATCTGTCGATGAACGGCC
GCTCGGTGGCGAGGGTGAAGTATCTGCTGCGCCGCATTGATATCGAAGAAGCGCAGGAGCTGTCGCGTCGCAGCCTCGAT
GCGCAGATGACCGCCGAGGTGCGCCATCAGGTGGCGGCCTTTATGGAGCGTCGCGGTCTCGGCGGCCTGATCCGCGGCGG
GCGGTAG

Upstream 100 bases:

>100_bases
GAAAGAGTTCGCCAGCGTGACCATGTCGCTGGCGGAGAGCGCACCTAAGCCGCAAAACGCGCCTGCTTATCGACGTAAAA
GAGGTTAAGCCACGCACATT

Downstream 100 bases:

>100_bases
CGCCAGCCCGGGTGAGGCGTAAGCCGTAACCCGGGGATCCTCTGCTCGCGCCCTCCGGCAAAGTAGGGTACCGCCGGGAA
AACGGCATTCTGTTAGCATC

Product: fused phosphoenolpyruvate-protein phosphotransferase PtsP/GAF domain

Products: NA

Alternate protein names: Enzyme I-Ntr; Phosphotransferase system, enzyme I [H]

Number of amino acids: Translated: 748; Mature: 748

Protein sequence:

>748_residues
MLTRLREIVEKVASAPRLNEALDILVTDVCQAMETEVCSVYLADNDRRCYYLMATRGLKKPRGRTVALAFDEGLVGLVGR
LAEPINLADAQKHPSFKYIPAVKEDRFRAFLGVPIIQRRQLLGVLVVQQRELRQFDESEESFLVTLATQMAAILSQSQLN
ALFGQYRQTRIRALPASSGVAIAEGWMDVSLPLMEQVYEASTLDTASERERLTGALEEAANEFRRYSKRYAAGAQKETAA
IFDLYSHLLSDARLRRELFAEVDKGAVAEWAVKKIIEKFAEQFAALSDGYLKERAGDLRTLGQRLLFHLDDSIQGPNTWP
ARIILVADELSATTLAEVPQDRLAGVVVRDGAANSHAAIMVRALGIPTVMGADIQPSLLHGHTLIVDGYRGELLVDPEPV
LLQEYQRLISEENELSRLAEDDLQRASELKSGERVKVMLNAGLSPEHEEKLGSFVDGIGLYRTEIPFMLQSGFPSEEEQV
AQYQGMLQMFNSKPVTLRTLDIGADKQLPYMPISEENPCLGWRGIRITLDQPEIFLIQVRAMLRANAATGNLSILLPMVT
SLEEVDEARRLIDRASREVEEMIGYAIPRPRLGVMLEVPSMVFMLPQLASRIDFISVGTNDLTQYLLAVDRNNTRVASMY
DSLHPAVLRALAMIAHDAERFGIDLRLCGEMAGDPMCVTILIGLGYRHLSMNGRSVARVKYLLRRIDIEEAQELSRRSLD
AQMTAEVRHQVAAFMERRGLGGLIRGGR

Sequences:

>Translated_748_residues
MLTRLREIVEKVASAPRLNEALDILVTDVCQAMETEVCSVYLADNDRRCYYLMATRGLKKPRGRTVALAFDEGLVGLVGR
LAEPINLADAQKHPSFKYIPAVKEDRFRAFLGVPIIQRRQLLGVLVVQQRELRQFDESEESFLVTLATQMAAILSQSQLN
ALFGQYRQTRIRALPASSGVAIAEGWMDVSLPLMEQVYEASTLDTASERERLTGALEEAANEFRRYSKRYAAGAQKETAA
IFDLYSHLLSDARLRRELFAEVDKGAVAEWAVKKIIEKFAEQFAALSDGYLKERAGDLRTLGQRLLFHLDDSIQGPNTWP
ARIILVADELSATTLAEVPQDRLAGVVVRDGAANSHAAIMVRALGIPTVMGADIQPSLLHGHTLIVDGYRGELLVDPEPV
LLQEYQRLISEENELSRLAEDDLQRASELKSGERVKVMLNAGLSPEHEEKLGSFVDGIGLYRTEIPFMLQSGFPSEEEQV
AQYQGMLQMFNSKPVTLRTLDIGADKQLPYMPISEENPCLGWRGIRITLDQPEIFLIQVRAMLRANAATGNLSILLPMVT
SLEEVDEARRLIDRASREVEEMIGYAIPRPRLGVMLEVPSMVFMLPQLASRIDFISVGTNDLTQYLLAVDRNNTRVASMY
DSLHPAVLRALAMIAHDAERFGIDLRLCGEMAGDPMCVTILIGLGYRHLSMNGRSVARVKYLLRRIDIEEAQELSRRSLD
AQMTAEVRHQVAAFMERRGLGGLIRGGR
>Mature_748_residues
MLTRLREIVEKVASAPRLNEALDILVTDVCQAMETEVCSVYLADNDRRCYYLMATRGLKKPRGRTVALAFDEGLVGLVGR
LAEPINLADAQKHPSFKYIPAVKEDRFRAFLGVPIIQRRQLLGVLVVQQRELRQFDESEESFLVTLATQMAAILSQSQLN
ALFGQYRQTRIRALPASSGVAIAEGWMDVSLPLMEQVYEASTLDTASERERLTGALEEAANEFRRYSKRYAAGAQKETAA
IFDLYSHLLSDARLRRELFAEVDKGAVAEWAVKKIIEKFAEQFAALSDGYLKERAGDLRTLGQRLLFHLDDSIQGPNTWP
ARIILVADELSATTLAEVPQDRLAGVVVRDGAANSHAAIMVRALGIPTVMGADIQPSLLHGHTLIVDGYRGELLVDPEPV
LLQEYQRLISEENELSRLAEDDLQRASELKSGERVKVMLNAGLSPEHEEKLGSFVDGIGLYRTEIPFMLQSGFPSEEEQV
AQYQGMLQMFNSKPVTLRTLDIGADKQLPYMPISEENPCLGWRGIRITLDQPEIFLIQVRAMLRANAATGNLSILLPMVT
SLEEVDEARRLIDRASREVEEMIGYAIPRPRLGVMLEVPSMVFMLPQLASRIDFISVGTNDLTQYLLAVDRNNTRVASMY
DSLHPAVLRALAMIAHDAERFGIDLRLCGEMAGDPMCVTILIGLGYRHLSMNGRSVARVKYLLRRIDIEEAQELSRRSLD
AQMTAEVRHQVAAFMERRGLGGLIRGGR

Specific function: Component of the phosphoenolpyruvate-dependent nitrogen- metabolic phosphotransferase system (nitrogen-metabolic PTS), that seems to be involved in regulating nitrogen metabolism. Enzyme I- Ntr transfers the phosphoryl group from phosphoenolpyruvate (PEP)

COG id: COG3605

COG function: function code T; Signal transduction protein containing GAF and PtsI domains

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GAF domain [H]

Homologues:

Organism=Escherichia coli, GI1789193, Length=747, Percent_Identity=89.2904953145917, Blast_Score=1371, Evalue=0.0,
Organism=Escherichia coli, GI1788756, Length=579, Percent_Identity=33.5060449050086, Blast_Score=283, Evalue=2e-77,
Organism=Escherichia coli, GI48994992, Length=506, Percent_Identity=33.9920948616601, Blast_Score=276, Evalue=3e-75,
Organism=Escherichia coli, GI1788726, Length=514, Percent_Identity=31.1284046692607, Blast_Score=239, Evalue=5e-64,
Organism=Escherichia coli, GI1787994, Length=406, Percent_Identity=25.3694581280788, Blast_Score=82, Evalue=2e-16,
Organism=Escherichia coli, GI226510935, Length=210, Percent_Identity=26.1904761904762, Blast_Score=69, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003018
- InterPro:   IPR008279
- InterPro:   IPR006318
- InterPro:   IPR018274
- InterPro:   IPR023151
- InterPro:   IPR000121
- InterPro:   IPR008731
- InterPro:   IPR015813 [H]

Pfam domain/function: PF01590 GAF; PF05524 PEP-utilisers_N; PF00391 PEP-utilizers; PF02896 PEP-utilizers_C [H]

EC number: =2.7.3.9 [H]

Molecular weight: Translated: 83507; Mature: 83507

Theoretical pI: Translated: 5.35; Mature: 5.35

Prosite motif: PS00370 PEP_ENZYMES_PHOS_SITE ; PS00742 PEP_ENZYMES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLTRLREIVEKVASAPRLNEALDILVTDVCQAMETEVCSVYLADNDRRCYYLMATRGLKK
CHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHEEEEEECCCCEEEEEEECCCCCC
PRGRTVALAFDEGLVGLVGRLAEPINLADAQKHPSFKYIPAVKEDRFRAFLGVPIIQRRQ
CCCCEEEEEECCCHHHHHHHHCCCCCCCCCCCCCCCEECCCCHHHHHHHHHCCCHHHHHH
LLGVLVVQQRELRQFDESEESFLVTLATQMAAILSQSQLNALFGQYRQTRIRALPASSGV
HHHHHHHHHHHHHHCCCCCHHEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
AIAEGWMDVSLPLMEQVYEASTLDTASERERLTGALEEAANEFRRYSKRYAAGAQKETAA
EEECCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHH
IFDLYSHLLSDARLRRELFAEVDKGAVAEWAVKKIIEKFAEQFAALSDGYLKERAGDLRT
HHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCHHHH
LGQRLLFHLDDSIQGPNTWPARIILVADELSATTLAEVPQDRLAGVVVRDGAANSHAAIM
HHHHHHHHHCCCCCCCCCCCEEEEEEECCCCHHHHHHCCHHHHCEEEEECCCCCCCCEEE
VRALGIPTVMGADIQPSLLHGHTLIVDGYRGELLVDPEPVLLQEYQRLISEENELSRLAE
EEECCCCCEECCCCCCHHCCCCEEEEECCCCCEEECCCHHHHHHHHHHHCCCHHHHHHHH
DDLQRASELKSGERVKVMLNAGLSPEHEEKLGSFVDGIGLYRTEIPFMLQSGFPSEEEQV
HHHHHHHHCCCCCEEEEEEECCCCCHHHHHHHHHHHCCHHHHHCCCHHHHCCCCCHHHHH
AQYQGMLQMFNSKPVTLRTLDIGADKQLPYMPISEENPCLGWRGIRITLDQPEIFLIQVR
HHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCEEEEEEEECCCCEEHHHHH
AMLRANAATGNLSILLPMVTSLEEVDEARRLIDRASREVEEMIGYAIPRPRLGVMLEVPS
HHHHHCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECHH
MVFMLPQLASRIDFISVGTNDLTQYLLAVDRNNTRVASMYDSLHPAVLRALAMIAHDAER
HHHHHHHHHHHCCEEECCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
FGIDLRLCGEMAGDPMCVTILIGLGYRHLSMNGRSVARVKYLLRRIDIEEAQELSRRSLD
HCCHHEEHHHCCCCCEEEHHHHHCCHHEECCCCCHHHHHHHHHHHCCHHHHHHHHHHHCC
AQMTAEVRHQVAAFMERRGLGGLIRGGR
HHHHHHHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure
MLTRLREIVEKVASAPRLNEALDILVTDVCQAMETEVCSVYLADNDRRCYYLMATRGLKK
CHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHEEEEEECCCCEEEEEEECCCCCC
PRGRTVALAFDEGLVGLVGRLAEPINLADAQKHPSFKYIPAVKEDRFRAFLGVPIIQRRQ
CCCCEEEEEECCCHHHHHHHHCCCCCCCCCCCCCCCEECCCCHHHHHHHHHCCCHHHHHH
LLGVLVVQQRELRQFDESEESFLVTLATQMAAILSQSQLNALFGQYRQTRIRALPASSGV
HHHHHHHHHHHHHHCCCCCHHEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
AIAEGWMDVSLPLMEQVYEASTLDTASERERLTGALEEAANEFRRYSKRYAAGAQKETAA
EEECCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHH
IFDLYSHLLSDARLRRELFAEVDKGAVAEWAVKKIIEKFAEQFAALSDGYLKERAGDLRT
HHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCHHHH
LGQRLLFHLDDSIQGPNTWPARIILVADELSATTLAEVPQDRLAGVVVRDGAANSHAAIM
HHHHHHHHHCCCCCCCCCCCEEEEEEECCCCHHHHHHCCHHHHCEEEEECCCCCCCCEEE
VRALGIPTVMGADIQPSLLHGHTLIVDGYRGELLVDPEPVLLQEYQRLISEENELSRLAE
EEECCCCCEECCCCCCHHCCCCEEEEECCCCCEEECCCHHHHHHHHHHHCCCHHHHHHHH
DDLQRASELKSGERVKVMLNAGLSPEHEEKLGSFVDGIGLYRTEIPFMLQSGFPSEEEQV
HHHHHHHHCCCCCEEEEEEECCCCCHHHHHHHHHHHCCHHHHHCCCHHHHCCCCCHHHHH
AQYQGMLQMFNSKPVTLRTLDIGADKQLPYMPISEENPCLGWRGIRITLDQPEIFLIQVR
HHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCEEEEEEEECCCCEEHHHHH
AMLRANAATGNLSILLPMVTSLEEVDEARRLIDRASREVEEMIGYAIPRPRLGVMLEVPS
HHHHHCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECHH
MVFMLPQLASRIDFISVGTNDLTQYLLAVDRNNTRVASMYDSLHPAVLRALAMIAHDAER
HHHHHHHHHHHCCEEECCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
FGIDLRLCGEMAGDPMCVTILIGLGYRHLSMNGRSVARVKYLLRRIDIEEAQELSRRSLD
HCCHHEEHHHCCCCCEEEHHHHHCCHHEECCCCCHHHHHHHHHHHCCHHHHHHHHHHHCC
AQMTAEVRHQVAAFMERRGLGGLIRGGR
HHHHHHHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9278503; 7896715; 8973315 [H]