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The map label for this gene is pduF [H]

Identifier: 238896307

GI number: 238896307

Start: 4286469

End: 4287278

Strand: Reverse

Name: pduF [H]

Synonym: KP1_4469

Alternate gene names: 238896307

Gene position: 4287278-4286469 (Counterclockwise)

Preceding gene: 238896308

Following gene: 238896306

Centisome position: 81.69

GC content: 57.9

Gene sequence:

>810_bases
ATGAATGGTTCTTTACGCGCGCAATGTATCGCCGAGTTTTTAGGCACCGGGCTGTTTCTGTTTTTTGGTATCTGCTGCCT
GAGCGCCCTGAAACTGACCGGCGCCAGCCTGGGGCTGTGGGAGATCTGTATTATCTGGGGACTGGGTATTTCTCTGGCGG
TGTACCTGACGGCCGGCATTTCGGGGGGACATCTGAACCCTGCGGTCACCGTCGCCCTGTGGCTGTTTGCCTGCTTCCCG
GGCAGAAAAGTGGTGCCCTATATCGTGGCTCAGGTCGCCGGCGCGTTTGGCGGCGCCGTGCTGGCCTGGATACTTTACAG
CACCCTGTTTACGCAATTTGAAACGGTGCATCATATGGTGCGCGGCAGCCTGGAAAGTCTGCAGCTGGCCAGTATATTCA
GTACCTACCCGGCGCCTGAGCTCAGCATCTGGCATGCGGCGCTGGTGGAGGTGGTGATCACCTCGATGCTGATGGGGATG
ATCATGGCGCTCACCGATGATGGCAACGGCGTACCCAAGGGCCCACTGGCGCCGCTGTTGATTGGCATTCTGGTGGCGGT
GATCGGCGCCTCGACCGGGCCGCTTACCGGCTTCGCGATGAATCCGGCACGCGATTTTGGCCCGAAATTGTTCACCTGGT
TCGCCGGGTGGGGCAATATCGCCATGACCGGGGGAAGAAATATTCCCTATTTTATCGTGCCCATTATCGCACCGTTGTTA
GGGGCCTGCCTCGGCGCCGCAATTTATCGTTTTCTTATCGCCAATAATCTGCCCTGTCATACCTGCGTGGAGGAAGAAAA
TACGCGTTAG

Upstream 100 bases:

>100_bases
CACTTTGCCGCTGCCGGTTTCTATAGTGGGCCTCGCCGTACCCAACGAGCCAGCGGATGGTTGGCTGGCCTGGACACGAA
CTTCGTCACGAGGTGTCACA

Downstream 100 bases:

>100_bases
CGTCATAAGGGGAATGTATCTGTGAAATAGCCAGGCTAATTTCATGAACCGATATCACGAACCGCTCAAAAAGTCTCCCC
GGGTTGGGAGACTTTTGTTA

Product: propanediol diffusion facilitator

Products: glycerol [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 269; Mature: 269

Protein sequence:

>269_residues
MNGSLRAQCIAEFLGTGLFLFFGICCLSALKLTGASLGLWEICIIWGLGISLAVYLTAGISGGHLNPAVTVALWLFACFP
GRKVVPYIVAQVAGAFGGAVLAWILYSTLFTQFETVHHMVRGSLESLQLASIFSTYPAPELSIWHAALVEVVITSMLMGM
IMALTDDGNGVPKGPLAPLLIGILVAVIGASTGPLTGFAMNPARDFGPKLFTWFAGWGNIAMTGGRNIPYFIVPIIAPLL
GACLGAAIYRFLIANNLPCHTCVEEENTR

Sequences:

>Translated_269_residues
MNGSLRAQCIAEFLGTGLFLFFGICCLSALKLTGASLGLWEICIIWGLGISLAVYLTAGISGGHLNPAVTVALWLFACFP
GRKVVPYIVAQVAGAFGGAVLAWILYSTLFTQFETVHHMVRGSLESLQLASIFSTYPAPELSIWHAALVEVVITSMLMGM
IMALTDDGNGVPKGPLAPLLIGILVAVIGASTGPLTGFAMNPARDFGPKLFTWFAGWGNIAMTGGRNIPYFIVPIIAPLL
GACLGAAIYRFLIANNLPCHTCVEEENTR
>Mature_269_residues
MNGSLRAQCIAEFLGTGLFLFFGICCLSALKLTGASLGLWEICIIWGLGISLAVYLTAGISGGHLNPAVTVALWLFACFP
GRKVVPYIVAQVAGAFGGAVLAWILYSTLFTQFETVHHMVRGSLESLQLASIFSTYPAPELSIWHAALVEVVITSMLMGM
IMALTDDGNGVPKGPLAPLLIGILVAVIGASTGPLTGFAMNPARDFGPKLFTWFAGWGNIAMTGGRNIPYFIVPIIAPLL
GACLGAAIYRFLIANNLPCHTCVEEENTR

Specific function: May facilitate the diffusion of propanediol [H]

COG id: COG0580

COG function: function code G; Glycerol uptake facilitator and related permeases (Major Intrinsic Protein Family)

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MIP/aquaporin (TC 1.A.8) family [H]

Homologues:

Organism=Homo sapiens, GI157266307, Length=260, Percent_Identity=36.9230769230769, Blast_Score=149, Evalue=2e-36,
Organism=Homo sapiens, GI22538420, Length=256, Percent_Identity=36.328125, Blast_Score=147, Evalue=1e-35,
Organism=Homo sapiens, GI4502187, Length=260, Percent_Identity=34.2307692307692, Blast_Score=142, Evalue=3e-34,
Organism=Homo sapiens, GI4826645, Length=273, Percent_Identity=36.2637362637363, Blast_Score=140, Evalue=1e-33,
Organism=Homo sapiens, GI310133356, Length=189, Percent_Identity=33.8624338624339, Blast_Score=95, Evalue=8e-20,
Organism=Homo sapiens, GI310114181, Length=189, Percent_Identity=33.8624338624339, Blast_Score=95, Evalue=8e-20,
Organism=Homo sapiens, GI45446752, Length=207, Percent_Identity=32.8502415458937, Blast_Score=81, Evalue=1e-15,
Organism=Homo sapiens, GI4502179, Length=243, Percent_Identity=32.5102880658436, Blast_Score=73, Evalue=3e-13,
Organism=Homo sapiens, GI4502183, Length=246, Percent_Identity=31.3008130081301, Blast_Score=70, Evalue=3e-12,
Organism=Homo sapiens, GI6912506, Length=243, Percent_Identity=30.4526748971193, Blast_Score=69, Evalue=6e-12,
Organism=Homo sapiens, GI86792455, Length=247, Percent_Identity=31.5789473684211, Blast_Score=68, Evalue=8e-12,
Organism=Escherichia coli, GI1790362, Length=267, Percent_Identity=66.2921348314607, Blast_Score=377, Evalue=1e-106,
Organism=Escherichia coli, GI1787101, Length=219, Percent_Identity=29.6803652968037, Blast_Score=69, Evalue=5e-13,
Organism=Caenorhabditis elegans, GI71992966, Length=254, Percent_Identity=34.251968503937, Blast_Score=140, Evalue=5e-34,
Organism=Caenorhabditis elegans, GI71994009, Length=273, Percent_Identity=34.0659340659341, Blast_Score=139, Evalue=2e-33,
Organism=Caenorhabditis elegans, GI17533613, Length=265, Percent_Identity=34.7169811320755, Blast_Score=130, Evalue=5e-31,
Organism=Caenorhabditis elegans, GI32564052, Length=265, Percent_Identity=34.7169811320755, Blast_Score=130, Evalue=6e-31,
Organism=Caenorhabditis elegans, GI17544068, Length=267, Percent_Identity=37.0786516853933, Blast_Score=130, Evalue=8e-31,
Organism=Caenorhabditis elegans, GI17531431, Length=263, Percent_Identity=33.0798479087452, Blast_Score=117, Evalue=8e-27,
Organism=Caenorhabditis elegans, GI17531429, Length=263, Percent_Identity=33.0798479087452, Blast_Score=116, Evalue=9e-27,
Organism=Caenorhabditis elegans, GI71992961, Length=254, Percent_Identity=31.496062992126, Blast_Score=106, Evalue=1e-23,
Organism=Caenorhabditis elegans, GI71993722, Length=253, Percent_Identity=28.0632411067194, Blast_Score=76, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI212646268, Length=280, Percent_Identity=25.3571428571429, Blast_Score=67, Evalue=8e-12,
Organism=Caenorhabditis elegans, GI17558372, Length=263, Percent_Identity=26.9961977186312, Blast_Score=65, Evalue=4e-11,
Organism=Saccharomyces cerevisiae, GI6321054, Length=212, Percent_Identity=38.6792452830189, Blast_Score=137, Evalue=3e-33,
Organism=Saccharomyces cerevisiae, GI6322985, Length=286, Percent_Identity=27.972027972028, Blast_Score=93, Evalue=5e-20,
Organism=Drosophila melanogaster, GI45550503, Length=260, Percent_Identity=23.8461538461538, Blast_Score=72, Evalue=3e-13,
Organism=Drosophila melanogaster, GI24762344, Length=202, Percent_Identity=29.2079207920792, Blast_Score=68, Evalue=6e-12,
Organism=Drosophila melanogaster, GI24762346, Length=202, Percent_Identity=29.2079207920792, Blast_Score=67, Evalue=9e-12,
Organism=Drosophila melanogaster, GI24762348, Length=202, Percent_Identity=29.2079207920792, Blast_Score=67, Evalue=9e-12,
Organism=Drosophila melanogaster, GI20130305, Length=202, Percent_Identity=29.2079207920792, Blast_Score=67, Evalue=9e-12,
Organism=Drosophila melanogaster, GI24652747, Length=253, Percent_Identity=25.296442687747, Blast_Score=64, Evalue=7e-11,
Organism=Drosophila melanogaster, GI45551084, Length=253, Percent_Identity=25.296442687747, Blast_Score=64, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012269
- InterPro:   IPR000425
- InterPro:   IPR022357 [H]

Pfam domain/function: PF00230 MIP [H]

EC number: NA

Molecular weight: Translated: 28508; Mature: 28508

Theoretical pI: Translated: 6.98; Mature: 6.98

Prosite motif: PS00221 MIP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.0 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
3.0 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
5.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHHHHCCCCCHHCCCCCCCC
>Mature Secondary Structure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HHHHHHHHHHHHHHCCCCCHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: glycerol [Periplasm] [C]

Specific reaction: glycerol [Periplasm] = glycerol [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8071226; 9352910; 11677609 [H]