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The map label for this gene is gudX [H]

Identifier: 238896250

GI number: 238896250

Start: 4227945

End: 4229285

Strand: Reverse

Name: gudX [H]

Synonym: KP1_4403

Alternate gene names: 238896250

Gene position: 4229285-4227945 (Counterclockwise)

Preceding gene: 238896251

Following gene: 238896249

Centisome position: 80.58

GC content: 63.83

Gene sequence:

>1341_bases
ATGACTACTCAATCCAGCCCGATTATCACAGAGATGAAGGTCATTCCGGTGGCCGGGCACGACAGCATGCTGCTGAACAT
CGGCGGAGCGCATAACGCATGGTTTACCCGCAATATCGTGGTGTTAACCGATAACGCCGGCCATACCGGCGTCGGCGAAG
CGCCCGGCGGAGAGGTCATCTACCAGACGCTGCTGGCGGCGATCCCGCAGGTGGTGGGCCAGGAGGTCGCCCGACTCAAT
CGCGTGGTGCAGCAGGTGCACAAAGGCAATCAGGCGGCGGATTTCGACACCTTCGGCAAAGGGGCGTGGACCTTCGAACT
GAAGGTTAACGCGGTGGCGGCGCTGGAAGCGGCGCTGCTCGACCTGCTCGGCCAGGTGCTTAATGTGCCGGTCTGCGAGC
TGCTGGGCCCGGGTAAGCAGCGTGATGCCGTCACCGTGCTGGGCTACCTGTTCTATATCGGCGATCGGCAGAAAACCGAC
CTTGGTTATCTGGACCATACGCCGGGCGATCACGAATGGTACCGCCTGCGCCACCAGCAGGCTTTGAGCAGCGAGGCGGT
AGTGCGTCTGGCGGAAGCCGCCCAGGATCGCTACGGTTTCAAAGACTTTAAGCTCAAAGGCGGCGTGCTGCCCGGCGAGC
AGGAGATCGACACCGCGCGGGCGCTGAAAAAGCGTTTCCCGGATGCGCGCATCACCGTCGACCCTAACGGCGCCTGGCTG
CTGGATGAGGCCATCGCGCTGTGTAAAGGGCTGCAGGATGTTCTGACCTACGCCGAAGATCCGTGCGGCGCCGAGCAGGG
TTTCTCCGGCCGGGAGGTGATGGCCGAGTTCCGCCGCGCCACCGGGCTGCCGGTCGCCACCAATATGATAGCCACTAACT
GGCGCGAAATGGGCCATGCGGTGATGCTCAACGCCGTCGATATCCCGCTGGCGGACCCGCACTTCTGGACGCTCTCCGGC
GCGGTGCGGGTGGCGCAGCTGTGCGACGACTGGGGGCTCACCTGGGGCTGCCATTCCAACAACCACTTTGATATTTCCCT
GGCGATGTTTACCCACGTCGGCGCCGCCGCGCCGGGCAACCCCACCGCCATCGATACCCACTGGATCTGGCAGGAGGGCG
ACGCGCGCCTGACCAAAAATCCGCTGCAGATTATTAACGGCAGCATTGCCGTGCCAGACGCGCCGGGACTCGGCGTGGAA
CTTGACTGGGAGCAGGTGCGCAGAGCCCATGAGGCCTATAAAGCGCTGCCGGGCGGCGCGCGCAACGACGCAGGCCCGAT
GCAGTACCTGATCCCCGGCTGGACATTCGACCGCAAACGTCCCGTTTTCGGCCGCCACTAA

Upstream 100 bases:

>100_bases
TCTACGTCGGTATCCATGCCCTGGTGGCGGTGCTGAGCTATCTGGTGCTGGTGGGCGATATCAAACGTATCGAACTGAAA
CCTGTAGCGGGACGTTAATC

Downstream 100 bases:

>100_bases
TTAAAAAGGATGATGCTATGACGACTTTCTCTTCAACGCCCGTTGTCACCACCATGCAGGTGATCCCGGTTGCCGGCCAT
GACAGCATGCTGATGAACCT

Product: putative (D)-glucarate dehydratase 2

Products: 5-dehydro-4-deoxy-D-glucarate; H2O

Alternate protein names: GDH-RP; GlucDRP [H]

Number of amino acids: Translated: 446; Mature: 445

Protein sequence:

>446_residues
MTTQSSPIITEMKVIPVAGHDSMLLNIGGAHNAWFTRNIVVLTDNAGHTGVGEAPGGEVIYQTLLAAIPQVVGQEVARLN
RVVQQVHKGNQAADFDTFGKGAWTFELKVNAVAALEAALLDLLGQVLNVPVCELLGPGKQRDAVTVLGYLFYIGDRQKTD
LGYLDHTPGDHEWYRLRHQQALSSEAVVRLAEAAQDRYGFKDFKLKGGVLPGEQEIDTARALKKRFPDARITVDPNGAWL
LDEAIALCKGLQDVLTYAEDPCGAEQGFSGREVMAEFRRATGLPVATNMIATNWREMGHAVMLNAVDIPLADPHFWTLSG
AVRVAQLCDDWGLTWGCHSNNHFDISLAMFTHVGAAAPGNPTAIDTHWIWQEGDARLTKNPLQIINGSIAVPDAPGLGVE
LDWEQVRRAHEAYKALPGGARNDAGPMQYLIPGWTFDRKRPVFGRH

Sequences:

>Translated_446_residues
MTTQSSPIITEMKVIPVAGHDSMLLNIGGAHNAWFTRNIVVLTDNAGHTGVGEAPGGEVIYQTLLAAIPQVVGQEVARLN
RVVQQVHKGNQAADFDTFGKGAWTFELKVNAVAALEAALLDLLGQVLNVPVCELLGPGKQRDAVTVLGYLFYIGDRQKTD
LGYLDHTPGDHEWYRLRHQQALSSEAVVRLAEAAQDRYGFKDFKLKGGVLPGEQEIDTARALKKRFPDARITVDPNGAWL
LDEAIALCKGLQDVLTYAEDPCGAEQGFSGREVMAEFRRATGLPVATNMIATNWREMGHAVMLNAVDIPLADPHFWTLSG
AVRVAQLCDDWGLTWGCHSNNHFDISLAMFTHVGAAAPGNPTAIDTHWIWQEGDARLTKNPLQIINGSIAVPDAPGLGVE
LDWEQVRRAHEAYKALPGGARNDAGPMQYLIPGWTFDRKRPVFGRH
>Mature_445_residues
TTQSSPIITEMKVIPVAGHDSMLLNIGGAHNAWFTRNIVVLTDNAGHTGVGEAPGGEVIYQTLLAAIPQVVGQEVARLNR
VVQQVHKGNQAADFDTFGKGAWTFELKVNAVAALEAALLDLLGQVLNVPVCELLGPGKQRDAVTVLGYLFYIGDRQKTDL
GYLDHTPGDHEWYRLRHQQALSSEAVVRLAEAAQDRYGFKDFKLKGGVLPGEQEIDTARALKKRFPDARITVDPNGAWLL
DEAIALCKGLQDVLTYAEDPCGAEQGFSGREVMAEFRRATGLPVATNMIATNWREMGHAVMLNAVDIPLADPHFWTLSGA
VRVAQLCDDWGLTWGCHSNNHFDISLAMFTHVGAAAPGNPTAIDTHWIWQEGDARLTKNPLQIINGSIAVPDAPGLGVEL
DWEQVRRAHEAYKALPGGARNDAGPMQYLIPGWTFDRKRPVFGRH

Specific function: Does not seem to have an in-vivo activity on glucarate or idarate. Its real substrate is unknown [H]

COG id: COG4948

COG function: function code MR; L-alanine-DL-glutamate epimerase and related enzymes of enolase superfamily

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mandelate racemase/muconate lactonizing enzyme family. GlucD subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789151, Length=446, Percent_Identity=91.4798206278027, Blast_Score=834, Evalue=0.0,
Organism=Escherichia coli, GI1789150, Length=448, Percent_Identity=63.1696428571429, Blast_Score=581, Evalue=1e-167,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018110
- InterPro:   IPR013342
- InterPro:   IPR013341
- InterPro:   IPR001354 [H]

Pfam domain/function: PF01188 MR_MLE; PF02746 MR_MLE_N [H]

EC number: 4.2.1.40

Molecular weight: Translated: 48714; Mature: 48583

Theoretical pI: Translated: 5.98; Mature: 5.98

Prosite motif: PS00908 MR_MLE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTQSSPIITEMKVIPVAGHDSMLLNIGGAHNAWFTRNIVVLTDNAGHTGVGEAPGGEVI
CCCCCCCCEEEEEEEEECCCCCEEEECCCCCCCEEEEEEEEEECCCCCCCCCCCCCHHHH
YQTLLAAIPQVVGQEVARLNRVVQQVHKGNQAADFDTFGKGAWTFELKVNAVAALEAALL
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEEEHHHHHHHHHHH
DLLGQVLNVPVCELLGPGKQRDAVTVLGYLFYIGDRQKTDLGYLDHTPGDHEWYRLRHQQ
HHHHHHHCCCHHHHCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHH
ALSSEAVVRLAEAAQDRYGFKDFKLKGGVLPGEQEIDTARALKKRFPDARITVDPNGAWL
HHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCHHHHHHHHHHHHHCCCCEEEECCCCCCH
LDEAIALCKGLQDVLTYAEDPCGAEQGFSGREVMAEFRRATGLPVATNMIATNWREMGHA
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCE
VMLNAVDIPLADPHFWTLSGAVRVAQLCDDWGLTWGCHSNNHFDISLAMFTHVGAAAPGN
EEEEEEECCCCCCCEEEHHHHHHHHHHHHHCCCEEEECCCCEEEEEEEHHHHCCCCCCCC
PTAIDTHWIWQEGDARLTKNPLQIINGSIAVPDAPGLGVELDWEQVRRAHEAYKALPGGA
CCEEEEEEEEECCCCCCCCCCHHHHCCCEECCCCCCCCEEECHHHHHHHHHHHHHCCCCC
RNDAGPMQYLIPGWTFDRKRPVFGRH
CCCCCCCEEEECCCCCCCCCCCCCCC
>Mature Secondary Structure 
TTQSSPIITEMKVIPVAGHDSMLLNIGGAHNAWFTRNIVVLTDNAGHTGVGEAPGGEVI
CCCCCCCEEEEEEEEECCCCCEEEECCCCCCCEEEEEEEEEECCCCCCCCCCCCCHHHH
YQTLLAAIPQVVGQEVARLNRVVQQVHKGNQAADFDTFGKGAWTFELKVNAVAALEAALL
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEEEHHHHHHHHHHH
DLLGQVLNVPVCELLGPGKQRDAVTVLGYLFYIGDRQKTDLGYLDHTPGDHEWYRLRHQQ
HHHHHHHCCCHHHHCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHH
ALSSEAVVRLAEAAQDRYGFKDFKLKGGVLPGEQEIDTARALKKRFPDARITVDPNGAWL
HHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCHHHHHHHHHHHHHCCCCEEEECCCCCCH
LDEAIALCKGLQDVLTYAEDPCGAEQGFSGREVMAEFRRATGLPVATNMIATNWREMGHA
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCE
VMLNAVDIPLADPHFWTLSGAVRVAQLCDDWGLTWGCHSNNHFDISLAMFTHVGAAAPGN
EEEEEEECCCCCCCEEEHHHHHHHHHHHHHCCCEEEECCCCEEEEEEEHHHHCCCCCCCC
PTAIDTHWIWQEGDARLTKNPLQIINGSIAVPDAPGLGVELDWEQVRRAHEAYKALPGGA
CCEEEEEEEEECCCCCCCCCCHHHHCCCEECCCCCCCCEEECHHHHHHHHHHHHHCCCCC
RNDAGPMQYLIPGWTFDRKRPVFGRH
CCCCCCCEEEECCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: D-glucarate

Specific reaction: D-glucarate = 5-dehydro-4-deoxy-D-glucarate + H2O

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9278503 [H]