Click here to switch to the map view.

The map label for this gene is cysG [H]

Identifier: 238896233

GI number: 238896233

Start: 4204328

End: 4205743

Strand: Reverse

Name: cysG [H]

Synonym: KP1_4385

Alternate gene names: 238896233

Gene position: 4205743-4204328 (Counterclockwise)

Preceding gene: 238896235

Following gene: 238896232

Centisome position: 80.13

GC content: 62.78

Gene sequence:

>1416_bases
GTGGATTACTTGCCCCTTTTTGCCGAACTGAAACAAAGGCCCGTGCTGGTGATCGGCGGCGGCGAGATTGCTGAACGTAA
GATCAAGTTCCTGCTGCGCGCCCAGGCGCAGGTGCAGGTGGTCGCTGAAACGCTGTCACCGGCGCTGGCCGATCTGGCTG
CGCGCCAGGCACTCAGCTGGCGGGCGACGGAATTCAGCGACTCGCTGGTGGATGATGTCTTTCTGGTGATTGCGGCCACC
GAGGATGAGGCGCTTAACCAGCGGGTATTTGCGGCAGCTAACGCGCGCTACCGGTTGGTCAACGTGGTGGATAACCAGGC
GCTGTGCTCGTTTGTTTTCCCTTCTATCGTCGACCGTTCGCCGCTGCTGGTGGCGATCTCCTCCAGCGGTAAAGCGCCGG
TGCTGTCGCGCATTCTGCGTGAAAAAATCGAAGCGCTGCTGCCGACGAATCTCGGTCGGCTGGCGGAATCAGCAAGCTAC
TGGCGCAACCATCTGAAAACCCGCCTGACGACTACCGAAGCGCGTCGCCGCTTCTGGGAGCGCGTCTTTACCGGCCGCTT
CGCCAGCCTGATGGTGGCGGGCAACTCTGCGGAGGCGGAAAAGGCGCTGCAGGATGAGTTGGATAAGCCCGAACGCGAGA
CGGGAGAAATTATTCTGGTCGGCGCCGGGCCGGGCGATGCCGGGCTGCTGACCCTTCGCGGTCTGCAGGCGATCCAGCAG
GCGGACGTGGTTTTCCACGACCATCTGGTGACTCAGCCGGTTCTCGAGCTGGTGCGCCGCGATGCCGAACTTATTTGCGT
CGGCAAACGCGCCGGCGAGCATTCGGTGCCGCAGCATGAAACCAATCAGCTGCTGGTGGAGGCGGCGAAGGCGGGGAAAA
CCGTCGTGCGCCTGAAGGGCGGCGATCCGTTTATTTTTGGCCGCGGCGCAGAAGAGCTGCAGGCTGCCGCCGAGGCGGGG
ATCCCGTTCCAGGTGGTGCCTGGCGTCACCGCAGCCGCCGGAGCGACGGCCTACGCCGGTATTCCGCTGACCCATCGCGA
TTATGCGCAAAGCGCCGTCTTTGTCACCGGCCACTATAAGCCGGACAGCGCCCCCTTCGACTGGTCGCTGCTGGCGAAGA
GCCAACAGACGCTGGCGATCTACATGGGCACCATGAAGGCAGCGGAAATCAGCGCCCAGCTGATCGCCCACGGTCGCGAC
AGCGACACGCCGGTGGCGGTAATTTCCCGCGGCACCCGTGACGATCAGCAGACGATAACCGGCACATTGCAACAACTCGA
ACACCTGGCGAAAGACGCCCCGATGCCCGCCCTGCTGGTGGTAGGCGAAGTGGTGCAGCTGCATCAGCAGCTCGCCTGGT
TTCAACATACATCATCCGCCGAGGGGTTTAACGCTTCGGTGGTCAATCTGGCTTAA

Upstream 100 bases:

>100_bases
TATTCCTTTGTGTCACAACTCATTCCATTTCGTAATTTCATTTGCTCTAAGGCGCACCCTATAGTCGCAGTATCGCTGAA
TGTAAGAGAGTTGTTGCACC

Downstream 100 bases:

>100_bases
GGAACGGTTATGGACCAAAAACGACTCACTCACCTGCGGCAACTGGAGGCGGAAAGCATCCATATTATTCGTGAGGTGGC
CGCCGAATTTTCTAACCCGG

Product: siroheme synthetase

Products: NA

Alternate protein names: Uroporphyrinogen-III C-methyltransferase; Urogen III methylase; SUMT; Uroporphyrinogen III methylase; UROM; Precorrin-2 dehydrogenase; Sirohydrochlorin ferrochelatase [H]

Number of amino acids: Translated: 471; Mature: 471

Protein sequence:

>471_residues
MDYLPLFAELKQRPVLVIGGGEIAERKIKFLLRAQAQVQVVAETLSPALADLAARQALSWRATEFSDSLVDDVFLVIAAT
EDEALNQRVFAAANARYRLVNVVDNQALCSFVFPSIVDRSPLLVAISSSGKAPVLSRILREKIEALLPTNLGRLAESASY
WRNHLKTRLTTTEARRRFWERVFTGRFASLMVAGNSAEAEKALQDELDKPERETGEIILVGAGPGDAGLLTLRGLQAIQQ
ADVVFHDHLVTQPVLELVRRDAELICVGKRAGEHSVPQHETNQLLVEAAKAGKTVVRLKGGDPFIFGRGAEELQAAAEAG
IPFQVVPGVTAAAGATAYAGIPLTHRDYAQSAVFVTGHYKPDSAPFDWSLLAKSQQTLAIYMGTMKAAEISAQLIAHGRD
SDTPVAVISRGTRDDQQTITGTLQQLEHLAKDAPMPALLVVGEVVQLHQQLAWFQHTSSAEGFNASVVNLA

Sequences:

>Translated_471_residues
MDYLPLFAELKQRPVLVIGGGEIAERKIKFLLRAQAQVQVVAETLSPALADLAARQALSWRATEFSDSLVDDVFLVIAAT
EDEALNQRVFAAANARYRLVNVVDNQALCSFVFPSIVDRSPLLVAISSSGKAPVLSRILREKIEALLPTNLGRLAESASY
WRNHLKTRLTTTEARRRFWERVFTGRFASLMVAGNSAEAEKALQDELDKPERETGEIILVGAGPGDAGLLTLRGLQAIQQ
ADVVFHDHLVTQPVLELVRRDAELICVGKRAGEHSVPQHETNQLLVEAAKAGKTVVRLKGGDPFIFGRGAEELQAAAEAG
IPFQVVPGVTAAAGATAYAGIPLTHRDYAQSAVFVTGHYKPDSAPFDWSLLAKSQQTLAIYMGTMKAAEISAQLIAHGRD
SDTPVAVISRGTRDDQQTITGTLQQLEHLAKDAPMPALLVVGEVVQLHQQLAWFQHTSSAEGFNASVVNLA
>Mature_471_residues
MDYLPLFAELKQRPVLVIGGGEIAERKIKFLLRAQAQVQVVAETLSPALADLAARQALSWRATEFSDSLVDDVFLVIAAT
EDEALNQRVFAAANARYRLVNVVDNQALCSFVFPSIVDRSPLLVAISSSGKAPVLSRILREKIEALLPTNLGRLAESASY
WRNHLKTRLTTTEARRRFWERVFTGRFASLMVAGNSAEAEKALQDELDKPERETGEIILVGAGPGDAGLLTLRGLQAIQQ
ADVVFHDHLVTQPVLELVRRDAELICVGKRAGEHSVPQHETNQLLVEAAKAGKTVVRLKGGDPFIFGRGAEELQAAAEAG
IPFQVVPGVTAAAGATAYAGIPLTHRDYAQSAVFVTGHYKPDSAPFDWSLLAKSQQTLAIYMGTMKAAEISAQLIAHGRD
SDTPVAVISRGTRDDQQTITGTLQQLEHLAKDAPMPALLVVGEVVQLHQQLAWFQHTSSAEGFNASVVNLA

Specific function: Multifunctional enzyme that catalyzes the SAM-dependent methylation of uroporphyrinogen III at position C-2 and C-7 to form precorrin-2 and then position C-12 or C-18 to form trimethylpyrrocorphin 2. It also catalyzes the conversion of precorrin-2 into si

COG id: COG0007

COG function: function code H; Uroporphyrinogen-III methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the precorrin methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1789768, Length=457, Percent_Identity=51.4223194748359, Blast_Score=464, Evalue=1e-132,
Organism=Saccharomyces cerevisiae, GI6322922, Length=257, Percent_Identity=33.4630350194552, Blast_Score=132, Evalue=1e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000878
- InterPro:   IPR014777
- InterPro:   IPR014776
- InterPro:   IPR006366
- InterPro:   IPR016040
- InterPro:   IPR019478
- InterPro:   IPR006367
- InterPro:   IPR003043 [H]

Pfam domain/function: PF10414 CysG_dimeriser; PF00590 TP_methylase [H]

EC number: =2.1.1.107; =1.3.1.76; =4.99.1.4 [H]

Molecular weight: Translated: 51001; Mature: 51001

Theoretical pI: Translated: 6.30; Mature: 6.30

Prosite motif: PS00839 SUMT_1 ; PS00840 SUMT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDYLPLFAELKQRPVLVIGGGEIAERKIKFLLRAQAQVQVVAETLSPALADLAARQALSW
CCCCCHHHHHHHCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
RATEFSDSLVDDVFLVIAATEDEALNQRVFAAANARYRLVNVVDNQALCSFVFPSIVDRS
CCHHHHHHHHCCEEEEEEECCCHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHHHHCCC
PLLVAISSSGKAPVLSRILREKIEALLPTNLGRLAESASYWRNHLKTRLTTTEARRRFWE
CEEEEECCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RVFTGRFASLMVAGNSAEAEKALQDELDKPERETGEIILVGAGPGDAGLLTLRGLQAIQQ
HHHHCCCEEEEEECCCHHHHHHHHHHCCCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHH
ADVVFHDHLVTQPVLELVRRDAELICVGKRAGEHSVPQHETNQLLVEAAKAGKTVVRLKG
HHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHCCCEEEEEEC
GDPFIFGRGAEELQAAAEAGIPFQVVPGVTAAAGATAYAGIPLTHRDYAQSAVFVTGHYK
CCEEEECCCHHHHHHHHHCCCCEEECCCCCHHCCCCCCCCCCCCCHHHCCCEEEEEECCC
PDSAPFDWSLLAKSQQTLAIYMGTMKAAEISAQLIAHGRDSDTPVAVISRGTRDDQQTIT
CCCCCCCHHHHHCCCCEEEEEECCHHHHHHHHHHHHCCCCCCCCEEEEECCCCCHHHHHH
GTLQQLEHLAKDAPMPALLVVGEVVQLHQQLAWFQHTSSAEGFNASVVNLA
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEECC
>Mature Secondary Structure
MDYLPLFAELKQRPVLVIGGGEIAERKIKFLLRAQAQVQVVAETLSPALADLAARQALSW
CCCCCHHHHHHHCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
RATEFSDSLVDDVFLVIAATEDEALNQRVFAAANARYRLVNVVDNQALCSFVFPSIVDRS
CCHHHHHHHHCCEEEEEEECCCHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHHHHCCC
PLLVAISSSGKAPVLSRILREKIEALLPTNLGRLAESASYWRNHLKTRLTTTEARRRFWE
CEEEEECCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RVFTGRFASLMVAGNSAEAEKALQDELDKPERETGEIILVGAGPGDAGLLTLRGLQAIQQ
HHHHCCCEEEEEECCCHHHHHHHHHHCCCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHH
ADVVFHDHLVTQPVLELVRRDAELICVGKRAGEHSVPQHETNQLLVEAAKAGKTVVRLKG
HHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHCCCEEEEEEC
GDPFIFGRGAEELQAAAEAGIPFQVVPGVTAAAGATAYAGIPLTHRDYAQSAVFVTGHYK
CCEEEECCCHHHHHHHHHCCCCEEECCCCCHHCCCCCCCCCCCCCHHHCCCEEEEEECCC
PDSAPFDWSLLAKSQQTLAIYMGTMKAAEISAQLIAHGRDSDTPVAVISRGTRDDQQTIT
CCCCCCCHHHHHCCCCEEEEEECCHHHHHHHHHHHHCCCCCCCCEEEEECCCCCHHHHHH
GTLQQLEHLAKDAPMPALLVVGEVVQLHQQLAWFQHTSSAEGFNASVVNLA
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA