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The map label for this gene is cysN [H]

Identifier: 238896231

GI number: 238896231

Start: 4201973

End: 4203400

Strand: Reverse

Name: cysN [H]

Synonym: KP1_4383

Alternate gene names: 238896231

Gene position: 4203400-4201973 (Counterclockwise)

Preceding gene: 238896232

Following gene: 238896230

Centisome position: 80.09

GC content: 60.78

Gene sequence:

>1428_bases
ATGAACACGACCATTGCCCAACAAATTGCCAACGAAGGCGGCGTAGAAGCTTACCTGCACGCGCAACAGCACAAGAGCCT
GCTGCGTTTTTTGACCTGCGGCAGCGTCGATGACGGCAAAAGCACCTTAATTGGCCGCCTGCTGCATGATACGCGCCAGA
TCTATGAAGACCAGCTCTCTTCGCTGCACAACGACAGCAAGCGTCACGGCACCCAGGGCGAAAAGCTGGACCTGGCGCTG
CTGGTGGACGGCCTGCAGGCCGAGCGCGAGCAGGGCATCACCATCGACGTGGCCTATCGCTACTTCTCGACCGAAAAGCG
CAAATTTATCATCGCCGACACCCCGGGGCACGAGCAGTACACGCGCAATATGGCCACCGGCGCGTCCACCTGCGACCTGG
CGATCCTGCTGATCGATGCGCGCAAGGGCGTGCTCGACCAGACCCGCCGTCACAGCTTCATCTCGACGCTGCTGGGCATT
AAACACCTGGTAGTGGCGGTCAACAAAATGGATCTCGTCGAGTTTAGCGAAGCGCGCTTCAACGAGATCCGGGAAGATTA
TCTGACCTTTGCCGAGCAGCTGCCGGGCAACCTTGATATCCGCTTTGTGCCGTTGTCGGCGCTGGAAGGCGATAATGTCG
CCAGCCAGAGCGCGAATATGCCGTGGTACAGTGGCCCGACGCTGCTGGAAGTCCTCGAAACCGTAGAGATCCAGCGGGTG
GTCGAGAGCCAGCCGCTGCGCTTCCCGGTGCAGTATGTTAACCGTCCGAACCTCGATTTCCGCGGCTTCTCCGGCACGGT
CGCCTCCGGTACCGTGCAGGTTGGCCAGCGTCTGAAGGTGCTGCCGTCGGGCGTCGAATCGAGCGTGGCGCGTATCGTCA
CCTTCGACGGCGACCTGCAGGAAGCCGCCGCCGGCGAAGCCATTACCCTTGTGCTGAAAGATGAAATCGACATCAGTCGC
GGCGACCTGCTGGTTGACGCGCAAGCCTCGCTGCCGGCGGTACAAAGCGCCAGCATCGACGTGGTGTGGATGGCCGAACA
GCCGCTGACCCCGGGCCAGAGCTACGACATCAAAATCGCCGGCAAGAAGACCCGCGCTCGCGTCGACGCTATCCGCTACC
AGGTCGATATTAACAACCTGACCCAGCGTGAAGTCGAGTCTCTGCCGCTGAACGGCATCGGGCTGGTGGAGCTGACCTTT
GACGAACCTTTAGTGCTGGATCCGTATCAGCAAAACCCGGTGACCGGCGGGCTTATCTTTATCGATCGCCTGACCAACGT
CACCGTCGGCGCCGGGATGGTCAACGAGCCGCACCTGCAGGCATCGACTTCGGCATCACAGTACAGCGCCTTCGAGCTGG
AGCTGAACCAGCTGATCCGTAAACATTTCCCGCACTGGGACGCCCGCGACCTGCTGGGAGGCCAATAA

Upstream 100 bases:

>100_bases
GGTATCGACCACCAGCGAACGTCAGGGGCGCGTCATTGACCGCGACCAGGCCGGCTCGATGGAGCTGAAGAAACGTCAGG
GGTATTTCTAAGGAGCCGCC

Downstream 100 bases:

>100_bases
TGGCCCAGCATGACGAAAACGTCGTCTGGCATGCCCACCCTGTGACCCAACAGCAGCGGGAACAGCATCACGGCCATCGC
GGCGTGGTGCTGTGGTTTAC

Product: sulfate adenylyltransferase subunit 1

Products: NA

Alternate protein names: ATP-sulfurylase large subunit; Sulfate adenylate transferase; SAT [H]

Number of amino acids: Translated: 475; Mature: 475

Protein sequence:

>475_residues
MNTTIAQQIANEGGVEAYLHAQQHKSLLRFLTCGSVDDGKSTLIGRLLHDTRQIYEDQLSSLHNDSKRHGTQGEKLDLAL
LVDGLQAEREQGITIDVAYRYFSTEKRKFIIADTPGHEQYTRNMATGASTCDLAILLIDARKGVLDQTRRHSFISTLLGI
KHLVVAVNKMDLVEFSEARFNEIREDYLTFAEQLPGNLDIRFVPLSALEGDNVASQSANMPWYSGPTLLEVLETVEIQRV
VESQPLRFPVQYVNRPNLDFRGFSGTVASGTVQVGQRLKVLPSGVESSVARIVTFDGDLQEAAAGEAITLVLKDEIDISR
GDLLVDAQASLPAVQSASIDVVWMAEQPLTPGQSYDIKIAGKKTRARVDAIRYQVDINNLTQREVESLPLNGIGLVELTF
DEPLVLDPYQQNPVTGGLIFIDRLTNVTVGAGMVNEPHLQASTSASQYSAFELELNQLIRKHFPHWDARDLLGGQ

Sequences:

>Translated_475_residues
MNTTIAQQIANEGGVEAYLHAQQHKSLLRFLTCGSVDDGKSTLIGRLLHDTRQIYEDQLSSLHNDSKRHGTQGEKLDLAL
LVDGLQAEREQGITIDVAYRYFSTEKRKFIIADTPGHEQYTRNMATGASTCDLAILLIDARKGVLDQTRRHSFISTLLGI
KHLVVAVNKMDLVEFSEARFNEIREDYLTFAEQLPGNLDIRFVPLSALEGDNVASQSANMPWYSGPTLLEVLETVEIQRV
VESQPLRFPVQYVNRPNLDFRGFSGTVASGTVQVGQRLKVLPSGVESSVARIVTFDGDLQEAAAGEAITLVLKDEIDISR
GDLLVDAQASLPAVQSASIDVVWMAEQPLTPGQSYDIKIAGKKTRARVDAIRYQVDINNLTQREVESLPLNGIGLVELTF
DEPLVLDPYQQNPVTGGLIFIDRLTNVTVGAGMVNEPHLQASTSASQYSAFELELNQLIRKHFPHWDARDLLGGQ
>Mature_475_residues
MNTTIAQQIANEGGVEAYLHAQQHKSLLRFLTCGSVDDGKSTLIGRLLHDTRQIYEDQLSSLHNDSKRHGTQGEKLDLAL
LVDGLQAEREQGITIDVAYRYFSTEKRKFIIADTPGHEQYTRNMATGASTCDLAILLIDARKGVLDQTRRHSFISTLLGI
KHLVVAVNKMDLVEFSEARFNEIREDYLTFAEQLPGNLDIRFVPLSALEGDNVASQSANMPWYSGPTLLEVLETVEIQRV
VESQPLRFPVQYVNRPNLDFRGFSGTVASGTVQVGQRLKVLPSGVESSVARIVTFDGDLQEAAAGEAITLVLKDEIDISR
GDLLVDAQASLPAVQSASIDVVWMAEQPLTPGQSYDIKIAGKKTRARVDAIRYQVDINNLTQREVESLPLNGIGLVELTF
DEPLVLDPYQQNPVTGGLIFIDRLTNVTVGAGMVNEPHLQASTSASQYSAFELELNQLIRKHFPHWDARDLLGGQ

Specific function: May be the GTPase, regulating ATP sulfurylase activity [H]

COG id: COG2895

COG function: function code P; GTPases - Sulfate adenylate transferase subunit 1

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. CysN/nodQ subfamily [H]

Homologues:

Organism=Homo sapiens, GI4503475, Length=436, Percent_Identity=28.8990825688073, Blast_Score=173, Evalue=4e-43,
Organism=Homo sapiens, GI223555963, Length=442, Percent_Identity=30.316742081448, Blast_Score=170, Evalue=3e-42,
Organism=Homo sapiens, GI5729864, Length=442, Percent_Identity=30.316742081448, Blast_Score=169, Evalue=4e-42,
Organism=Homo sapiens, GI4503471, Length=436, Percent_Identity=27.9816513761468, Blast_Score=167, Evalue=2e-41,
Organism=Homo sapiens, GI194097354, Length=309, Percent_Identity=34.6278317152104, Blast_Score=163, Evalue=3e-40,
Organism=Homo sapiens, GI194018520, Length=309, Percent_Identity=34.6278317152104, Blast_Score=163, Evalue=3e-40,
Organism=Homo sapiens, GI194018522, Length=309, Percent_Identity=34.6278317152104, Blast_Score=163, Evalue=4e-40,
Organism=Homo sapiens, GI46094014, Length=309, Percent_Identity=33.6569579288026, Blast_Score=159, Evalue=6e-39,
Organism=Homo sapiens, GI34147630, Length=315, Percent_Identity=29.2063492063492, Blast_Score=97, Evalue=3e-20,
Organism=Escherichia coli, GI1789108, Length=473, Percent_Identity=88.3720930232558, Blast_Score=867, Evalue=0.0,
Organism=Escherichia coli, GI1790412, Length=318, Percent_Identity=27.0440251572327, Blast_Score=85, Evalue=8e-18,
Organism=Escherichia coli, GI1789737, Length=318, Percent_Identity=27.0440251572327, Blast_Score=85, Evalue=8e-18,
Organism=Escherichia coli, GI2367247, Length=329, Percent_Identity=26.4437689969605, Blast_Score=74, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17552884, Length=439, Percent_Identity=30.0683371298405, Blast_Score=176, Evalue=3e-44,
Organism=Caenorhabditis elegans, GI17569207, Length=439, Percent_Identity=30.0683371298405, Blast_Score=176, Evalue=3e-44,
Organism=Caenorhabditis elegans, GI32566629, Length=436, Percent_Identity=30.2752293577982, Blast_Score=166, Evalue=2e-41,
Organism=Caenorhabditis elegans, GI115532067, Length=342, Percent_Identity=29.2397660818713, Blast_Score=164, Evalue=9e-41,
Organism=Caenorhabditis elegans, GI115532065, Length=342, Percent_Identity=29.2397660818713, Blast_Score=164, Evalue=1e-40,
Organism=Caenorhabditis elegans, GI32566303, Length=432, Percent_Identity=28.7037037037037, Blast_Score=148, Evalue=6e-36,
Organism=Caenorhabditis elegans, GI25141371, Length=238, Percent_Identity=26.0504201680672, Blast_Score=92, Evalue=8e-19,
Organism=Caenorhabditis elegans, GI32566301, Length=138, Percent_Identity=36.9565217391304, Blast_Score=88, Evalue=9e-18,
Organism=Caenorhabditis elegans, GI17556456, Length=345, Percent_Identity=27.2463768115942, Blast_Score=85, Evalue=9e-17,
Organism=Saccharomyces cerevisiae, GI6325337, Length=434, Percent_Identity=28.8018433179723, Blast_Score=174, Evalue=2e-44,
Organism=Saccharomyces cerevisiae, GI6319594, Length=434, Percent_Identity=28.8018433179723, Blast_Score=174, Evalue=2e-44,
Organism=Saccharomyces cerevisiae, GI6322937, Length=449, Percent_Identity=29.3986636971047, Blast_Score=167, Evalue=4e-42,
Organism=Saccharomyces cerevisiae, GI6320377, Length=437, Percent_Identity=29.0617848970252, Blast_Score=140, Evalue=5e-34,
Organism=Saccharomyces cerevisiae, GI6324761, Length=298, Percent_Identity=27.8523489932886, Blast_Score=92, Evalue=2e-19,
Organism=Saccharomyces cerevisiae, GI6323320, Length=149, Percent_Identity=33.5570469798658, Blast_Score=64, Evalue=8e-11,
Organism=Drosophila melanogaster, GI24652838, Length=436, Percent_Identity=27.9816513761468, Blast_Score=165, Evalue=8e-41,
Organism=Drosophila melanogaster, GI17137572, Length=436, Percent_Identity=27.9816513761468, Blast_Score=165, Evalue=8e-41,
Organism=Drosophila melanogaster, GI45553807, Length=451, Percent_Identity=26.8292682926829, Blast_Score=161, Evalue=7e-40,
Organism=Drosophila melanogaster, GI45553816, Length=451, Percent_Identity=26.8292682926829, Blast_Score=161, Evalue=7e-40,
Organism=Drosophila melanogaster, GI24651721, Length=451, Percent_Identity=26.8292682926829, Blast_Score=161, Evalue=7e-40,
Organism=Drosophila melanogaster, GI17864154, Length=451, Percent_Identity=26.8292682926829, Blast_Score=161, Evalue=7e-40,
Organism=Drosophila melanogaster, GI45550900, Length=433, Percent_Identity=28.8683602771363, Blast_Score=159, Evalue=4e-39,
Organism=Drosophila melanogaster, GI17137380, Length=311, Percent_Identity=32.475884244373, Blast_Score=159, Evalue=6e-39,
Organism=Drosophila melanogaster, GI281363316, Length=309, Percent_Identity=28.1553398058252, Blast_Score=97, Evalue=2e-20,
Organism=Drosophila melanogaster, GI17864358, Length=309, Percent_Identity=28.1553398058252, Blast_Score=97, Evalue=2e-20,
Organism=Drosophila melanogaster, GI19921738, Length=261, Percent_Identity=28.735632183908, Blast_Score=83, Evalue=4e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000795
- InterPro:   IPR011779
- InterPro:   IPR009001
- InterPro:   IPR004161
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2 [H]

EC number: =2.7.7.4 [H]

Molecular weight: Translated: 52492; Mature: 52492

Theoretical pI: Translated: 4.82; Mature: 4.82

Prosite motif: PS00659 GLYCOSYL_HYDROL_F5 ; PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTTIAQQIANEGGVEAYLHAQQHKSLLRFLTCGSVDDGKSTLIGRLLHDTRQIYEDQLS
CCCHHHHHHHCCCCCEEEEEHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHH
SLHNDSKRHGTQGEKLDLALLVDGLQAEREQGITIDVAYRYFSTEKRKFIIADTPGHEQY
HHHCCHHHCCCCCCCEEEEEEECCHHHHHHCCCEEEEEEEEECCCCCEEEEEECCCCHHH
TRNMATGASTCDLAILLIDARKGVLDQTRRHSFISTLLGIKHLVVAVNKMDLVEFSEARF
HHHHHCCCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHH
NEIREDYLTFAEQLPGNLDIRFVPLSALEGDNVASQSANMPWYSGPTLLEVLETVEIQRV
HHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHH
VESQPLRFPVQYVNRPNLDFRGFSGTVASGTVQVGQRLKVLPSGVESSVARIVTFDGDLQ
HHCCCCCCCHHHCCCCCCCCCCCCCCCCCCHHHHCHHHHCCCCCHHHCEEEEEEECCCHH
EAAAGEAITLVLKDEIDISRGDLLVDAQASLPAVQSASIDVVWMAEQPLTPGQSYDIKIA
HHCCCCEEEEEEECCCCCCCCCEEEECCCCCCCCCCCCEEEEEEECCCCCCCCCEEEEEE
GKKTRARVDAIRYQVDINNLTQREVESLPLNGIGLVELTFDEPLVLDPYQQNPVTGGLIF
CCHHHHHEEEEEEEEECCHHHHHHHHHCCCCCCEEEEEECCCCEEECCCCCCCCCCCEEE
IDRLTNVTVGAGMVNEPHLQASTSASQYSAFELELNQLIRKHFPHWDARDLLGGQ
EEECCCEEECCCCCCCCCCCCCCCCCHHEHHHHHHHHHHHHHCCCCCHHHHCCCC
>Mature Secondary Structure
MNTTIAQQIANEGGVEAYLHAQQHKSLLRFLTCGSVDDGKSTLIGRLLHDTRQIYEDQLS
CCCHHHHHHHCCCCCEEEEEHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHH
SLHNDSKRHGTQGEKLDLALLVDGLQAEREQGITIDVAYRYFSTEKRKFIIADTPGHEQY
HHHCCHHHCCCCCCCEEEEEEECCHHHHHHCCCEEEEEEEEECCCCCEEEEEECCCCHHH
TRNMATGASTCDLAILLIDARKGVLDQTRRHSFISTLLGIKHLVVAVNKMDLVEFSEARF
HHHHHCCCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHH
NEIREDYLTFAEQLPGNLDIRFVPLSALEGDNVASQSANMPWYSGPTLLEVLETVEIQRV
HHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHH
VESQPLRFPVQYVNRPNLDFRGFSGTVASGTVQVGQRLKVLPSGVESSVARIVTFDGDLQ
HHCCCCCCCHHHCCCCCCCCCCCCCCCCCCHHHHCHHHHCCCCCHHHCEEEEEEECCCHH
EAAAGEAITLVLKDEIDISRGDLLVDAQASLPAVQSASIDVVWMAEQPLTPGQSYDIKIA
HHCCCCEEEEEEECCCCCCCCCEEEECCCCCCCCCCCCEEEEEEECCCCCCCCCEEEEEE
GKKTRARVDAIRYQVDINNLTQREVESLPLNGIGLVELTFDEPLVLDPYQQNPVTGGLIF
CCHHHHHEEEEEEEEECCHHHHHHHHHCCCCCCEEEEEECCCCEEECCCCCCCCCCCEEE
IDRLTNVTVGAGMVNEPHLQASTSASQYSAFELELNQLIRKHFPHWDARDLLGGQ
EEECCCEEECCCCCCCCCCCCCCCCCHHEHHHHHHHHHHHHHCCCCCHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA