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The map label for this gene is truD [H]

Identifier: 238896225

GI number: 238896225

Start: 4198278

End: 4199327

Strand: Reverse

Name: truD [H]

Synonym: KP1_4377

Alternate gene names: 238896225

Gene position: 4199327-4198278 (Counterclockwise)

Preceding gene: 238896226

Following gene: 238896224

Centisome position: 80.01

GC content: 59.81

Gene sequence:

>1050_bases
ATGATCGCCTTTGACCAACTGACCTGGCTGCACGGCAAGCCGCAGAGCAGTGGGCTGCTGAAAGCCAATCCGGAAGATTT
TCTGGTGGTGGAAGATCTGGGCTTTGCCCCGGACGGCGAAGGCGAACACGTGCTGGTGCGGATCCTGAAAAACGGCTGCA
ATACGCGCTTTGTCGCCGACGCGCTGGCGAAATTCCTTAAGATCCACGCCCGTGAAGTGAGCTTCGCCGGGCAAAAGGAT
AAGCACGCGGTCACCGAGCAGTGGCTGTGCGCCCGGGTGCCCGGCAAAGAGATGCCGGACCTGAGCAAATTTCAGCTCGA
GGGCTGCCAGGTACTGGAATATGCTCGCCATAAGCGTAAGCTGCGTTTAGGCGCGCTGAAAGGCAACCAGTTTACCGTGA
TCCTGCGCGAGATTAGCGATCGCCAGGACGTGGAGACGCGTCTGCAGGCCATCGCTGAACGGGGAGTGCCGAACTATTTC
GGCGCGCAGCGCTTTGGCATTGGCGGCAGTAACCTGCAGGGCGCCCTGCGCTGGGCGGAGAGCGGCGCGCCAGTGCGCGA
TCGCAATAAGCGCAGTTTTTGGTTGTCGGCAGCCCGCAGCGCGTTGTTTAATCAGCAAGTGAGTATTAGATTAAAAAAAA
CGGAATTTAATCAGGTCGTTGATGGCGATGCGCTACAATTAGCGGGGCGCGGGAGCTGGTTTGTCGTCACACCGGAAGAA
CTCGAGGTTTCGCAGGCGCGGGTGCATAACCGCGAACTGATGATTACGGCCACGCTGCCGGGCAGCGGCGACTGGGGAAG
CCAGCGCGACGCCCTGGCTTTCGAGCAGGCCGCGATCGCCGAGGAAACCGCGTTGCAGGCGCTGCTGGTACGGGAGAAAG
TCGAGGCGGCGCGCCGGGCGATGCTTCTCTATCCACAGCAGCTCAGCTGGAACTGGTGGGATGACGTAACCGTTGAACTA
CGTTTCTGGCTGCCGGCGGGCAGCTTCGCCACCAGCGTGGTCAGGGAGCTTATCAATACAACGGGTGACTATGCGAATAT
TGCTGAGTAA

Upstream 100 bases:

>100_bases
ACGTCAACGTGAAAGCGACCACCACCGAGAAGCTCGGCTTCACCGGCCGCGGGGAAGGGATCGCCTGCGAAGCGGTTGCG
CTGCTGCGTAAGGCCGACAA

Downstream 100 bases:

>100_bases
CGATGACGGGATCCATGCGCCGGGGATACAGACCCTGGCGAAGGCCTTAAGGGAGTTTGCCGAGGTCCAGGTGGTTGCAC
CCGATCGTAACCGCAGCGGG

Product: tRNA pseudouridine synthase D

Products: NA

Alternate protein names: tRNA pseudouridylate synthase D; tRNA-uridine isomerase D [H]

Number of amino acids: Translated: 349; Mature: 349

Protein sequence:

>349_residues
MIAFDQLTWLHGKPQSSGLLKANPEDFLVVEDLGFAPDGEGEHVLVRILKNGCNTRFVADALAKFLKIHAREVSFAGQKD
KHAVTEQWLCARVPGKEMPDLSKFQLEGCQVLEYARHKRKLRLGALKGNQFTVILREISDRQDVETRLQAIAERGVPNYF
GAQRFGIGGSNLQGALRWAESGAPVRDRNKRSFWLSAARSALFNQQVSIRLKKTEFNQVVDGDALQLAGRGSWFVVTPEE
LEVSQARVHNRELMITATLPGSGDWGSQRDALAFEQAAIAEETALQALLVREKVEAARRAMLLYPQQLSWNWWDDVTVEL
RFWLPAGSFATSVVRELINTTGDYANIAE

Sequences:

>Translated_349_residues
MIAFDQLTWLHGKPQSSGLLKANPEDFLVVEDLGFAPDGEGEHVLVRILKNGCNTRFVADALAKFLKIHAREVSFAGQKD
KHAVTEQWLCARVPGKEMPDLSKFQLEGCQVLEYARHKRKLRLGALKGNQFTVILREISDRQDVETRLQAIAERGVPNYF
GAQRFGIGGSNLQGALRWAESGAPVRDRNKRSFWLSAARSALFNQQVSIRLKKTEFNQVVDGDALQLAGRGSWFVVTPEE
LEVSQARVHNRELMITATLPGSGDWGSQRDALAFEQAAIAEETALQALLVREKVEAARRAMLLYPQQLSWNWWDDVTVEL
RFWLPAGSFATSVVRELINTTGDYANIAE
>Mature_349_residues
MIAFDQLTWLHGKPQSSGLLKANPEDFLVVEDLGFAPDGEGEHVLVRILKNGCNTRFVADALAKFLKIHAREVSFAGQKD
KHAVTEQWLCARVPGKEMPDLSKFQLEGCQVLEYARHKRKLRLGALKGNQFTVILREISDRQDVETRLQAIAERGVPNYF
GAQRFGIGGSNLQGALRWAESGAPVRDRNKRSFWLSAARSALFNQQVSIRLKKTEFNQVVDGDALQLAGRGSWFVVTPEE
LEVSQARVHNRELMITATLPGSGDWGSQRDALAFEQAAIAEETALQALLVREKVEAARRAMLLYPQQLSWNWWDDVTVEL
RFWLPAGSFATSVVRELINTTGDYANIAE

Specific function: Responsible for synthesis of pseudouridine from uracil- 13 in transfer RNAs [H]

COG id: COG0585

COG function: function code S; Uncharacterized conserved protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRUD domain [H]

Homologues:

Organism=Homo sapiens, GI50727002, Length=132, Percent_Identity=31.8181818181818, Blast_Score=74, Evalue=2e-13,
Organism=Escherichia coli, GI1789102, Length=349, Percent_Identity=85.6733524355301, Blast_Score=620, Evalue=1e-179,
Organism=Caenorhabditis elegans, GI17557232, Length=122, Percent_Identity=34.4262295081967, Blast_Score=65, Evalue=5e-11,
Organism=Saccharomyces cerevisiae, GI6324817, Length=144, Percent_Identity=31.25, Blast_Score=70, Evalue=4e-13,
Organism=Drosophila melanogaster, GI21355799, Length=132, Percent_Identity=31.8181818181818, Blast_Score=69, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR001656
- InterPro:   IPR020119
- InterPro:   IPR011760 [H]

Pfam domain/function: PF01142 TruD [H]

EC number: NA

Molecular weight: Translated: 39103; Mature: 39103

Theoretical pI: Translated: 7.60; Mature: 7.60

Prosite motif: PS50984 TRUD ; PS01268 UPF0024

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIAFDQLTWLHGKPQSSGLLKANPEDFLVVEDLGFAPDGEGEHVLVRILKNGCNTRFVAD
CCCCCCEEEECCCCCCCCCEECCCCCEEEEECCCCCCCCCCCHHHHHHHHCCCCCHHHHH
ALAKFLKIHAREVSFAGQKDKHAVTEQWLCARVPGKEMPDLSKFQLEGCQVLEYARHKRK
HHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
LRLGALKGNQFTVILREISDRQDVETRLQAIAERGVPNYFGAQRFGIGGSNLQGALRWAE
EEEEEECCCEEEEEEEHHCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHC
SGAPVRDRNKRSFWLSAARSALFNQQVSIRLKKTEFNQVVDGDALQLAGRGSWFVVTPEE
CCCCCCCCCCHHHHHHHHHHHHHCCEEEEEEEECHHHHHCCCCCEEEECCCCEEEECHHH
LEVSQARVHNRELMITATLPGSGDWGSQRDALAFEQAAIAEETALQALLVREKVEAARRA
HHHHHHHHCCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MLLYPQQLSWNWWDDVTVELRFWLPAGSFATSVVRELINTTGDYANIAE
HEECCHHCCCCCCCCEEEEEEEEECCCHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MIAFDQLTWLHGKPQSSGLLKANPEDFLVVEDLGFAPDGEGEHVLVRILKNGCNTRFVAD
CCCCCCEEEECCCCCCCCCEECCCCCEEEEECCCCCCCCCCCHHHHHHHHCCCCCHHHHH
ALAKFLKIHAREVSFAGQKDKHAVTEQWLCARVPGKEMPDLSKFQLEGCQVLEYARHKRK
HHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
LRLGALKGNQFTVILREISDRQDVETRLQAIAERGVPNYFGAQRFGIGGSNLQGALRWAE
EEEEEECCCEEEEEEEHHCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHC
SGAPVRDRNKRSFWLSAARSALFNQQVSIRLKKTEFNQVVDGDALQLAGRGSWFVVTPEE
CCCCCCCCCCHHHHHHHHHHHHHCCEEEEEEEECHHHHHCCCCCEEEECCCCEEEECHHH
LEVSQARVHNRELMITATLPGSGDWGSQRDALAFEQAAIAEETALQALLVREKVEAARRA
HHHHHHHHCCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MLLYPQQLSWNWWDDVTVELRFWLPAGSFATSVVRELINTTGDYANIAE
HEECCHHCCCCCCCCEEEEEEEEECCCHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA