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The map label for this gene is fhlA [H]

Identifier: 238896188

GI number: 238896188

Start: 4158467

End: 4160539

Strand: Direct

Name: fhlA [H]

Synonym: KP1_4336

Alternate gene names: 238896188

Gene position: 4158467-4160539 (Clockwise)

Preceding gene: 238896187

Following gene: 238896193

Centisome position: 79.23

GC content: 61.17

Gene sequence:

>2073_bases
ATGTCGTATACACCGATGAGCGATCTCGGCCAGCAGGGGCTGTTCGATATCACCCGGCTTTTATTACAGCAGCCCGATCT
GGCCGCGCTAAGCGAAACCCTGACCCGTCTGGTGCAACAGTCCGCGCTGGCCGATGAGGCGGCCATCATCCTGTGGAACG
CGGGTAATCACCGCGCGGCCAGGTACGCCTGCGATGAGGCCGGCCATCCGGTAAGCTACGAGGATGAAACCGTCCTGGCC
CACGGTCCGGTGCGTCGCTTACTCTCCCGGCCGGATGCCCTGCATTGCGATCATGAAACCTTTGCCGACACCTGGCCGCA
GCTTATCCGCAGCGGGCTCTATCGCCCGTTCGGATACTATTCGCTGCTGCCGCTGGCGGCGGACGGGCGGATCTTCGGCG
GTTGCGAATTCCTCCGCCGTGACAACCGGCCGTGGAGCGAGAAAGAGTTCCAGCGGCTGCATACCTTCGCGCAGATCGTC
GCCGTCGTCACCGAACAGATTCAGAACCGGGTCAGCAATAACGTCGATTACGATCTGCTGTGCCACGAGCGCGACAACTT
TCGCATCCTGGTGGCGATCACCAACGCGGTGCTCTCGCGACTGGATATCGACGAGCTGGTCAGCGAGGTGGCGAAAGAGA
TCCATCGCTACTTCCGGATCGACGCCATCAGCGTGGTGCTGCGCAGCGACCGCAAGGGGAAGCTCAACATCTATTCCACG
CATTATCTTGACGCCAGCCACCCGGTTCACGACCAGAGCGAGGTGGATGAAGCCGGCACCCTCACCGAGCGCGTGTTCAA
AAGCAAAGAGATGCTGCTGCTTAATCTGCACGAACATGACACTCTCGCGCCGTATGAGAAAATGCTCTTCGAGATGTGGG
GCAATAAGATCCAGACCCTGTGCCTGCTGCCGCTGATGTCCGGCAATACCCTGCTCGGGGTGCTTAAGCTGGCGCAGTGC
GACGAACAGGTCTTCACCACCACCAATCTCAAGCTGCTGCGGCAGATCGCCGAGCGGGTGTCGATCGCTATCGATAACGC
CCTCGCCTACCGCGAAATTCAGCGCCTGAAAGAGCGGCTGGTGGATGAGAACCTCGCCCTCACCGAGCAGCTCAATAATG
TCGAGAGCGAATTCGGGGAGATTATCGGCCGCAGCGAGGCGATGAACAATGTGCTCAAGCAGGTAGAGATGGTGGCGCAC
AGCGACAGCACCGTGCTGATCCTCGGCGAAACCGGCACCGGCAAAGAGCTTATCGCCCGCGCTATCCACAACCTCAGCGG
ACGCAACGGTCGGCGGATGGTGAAGATGAACTGCGCGGCGATGCCCGCCGGGCTGCTGGAGAGCGATCTGTTCGGCCACG
AACGCGGCGCGTTCACCGGGGCCAGCGCCCAGCGTATCGGCCGCTTCGAACTGGCGGACAAAAGCTCCCTGTTCCTCGAT
GAAGTGGGCGATATGCCGCTGGAACTGCAGCCCAAACTGCTGCGCGTCCTCCAGGAGCAGGAGTTCGAACGTCTGGGGAG
CAACAAGCTGATTCAGACCGACGTTCGATTGATCGCCGCCACCAACCGCGATCTCAAGCAGATGGTTATCGACAGGGAAT
TCCGCAGCGATCTCTACTACCGGCTCAACGTGTTCCCGATCCACCTGCCGCCGCTGCGCGAACGTCCCGACGATATCCCG
CTGCTGGTGAAGGCGTTTACCTTTAAAATCGCCCGCCGCATGGGGCGCAATATCGACAGCATTCCGGCGGAAACCCTGCG
CACTCTGACGCGTATGGAGTGGCCGGGCAACGTGCGCGAGCTGGAAAACGTTATCGAACGGGCGGTATTGCTCACCCGCG
GCAACGTACTGCAGCTCTCCCTGCCGGAGCGGGATATCGTGGAAGCCCCGCGGACGCCGGCGGTCCTGCCTGAGGAAGGT
GAGGATGAATATCAGCTGATCGTCCGGGTGCTGAAGGAGAGCAACGGCGTGGTGGCCGGGCCGAAAGGCGCCGCCCAGCG
GCTGGGGCTCAAGCGCACCACGCTCCTGTCACGCATGAAGCGGCTGGGTATTAATAAAGACGAACTGGTGTAA

Upstream 100 bases:

>100_bases
TGGATGGGTTAAAGCGGCAATAACCGCTTTATTAACATACTATTATTTGTACCATCATTTTCTTATGCGCCGCGTTGTCG
GCGCGTTTTGGACGTAAGCA

Downstream 100 bases:

>100_bases
CCTCGCGCGCTCCCCCTTTCCGCGCCGGAGAGAGGGAGCGCTGGCGCAGCGCGCTATTTATCGCTGGTAAAACCGTTGCT
GGACGCCAGTTCGGCAAACC

Product: formate hydrogen-lyase transcriptional activator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 690; Mature: 689

Protein sequence:

>690_residues
MSYTPMSDLGQQGLFDITRLLLQQPDLAALSETLTRLVQQSALADEAAIILWNAGNHRAARYACDEAGHPVSYEDETVLA
HGPVRRLLSRPDALHCDHETFADTWPQLIRSGLYRPFGYYSLLPLAADGRIFGGCEFLRRDNRPWSEKEFQRLHTFAQIV
AVVTEQIQNRVSNNVDYDLLCHERDNFRILVAITNAVLSRLDIDELVSEVAKEIHRYFRIDAISVVLRSDRKGKLNIYST
HYLDASHPVHDQSEVDEAGTLTERVFKSKEMLLLNLHEHDTLAPYEKMLFEMWGNKIQTLCLLPLMSGNTLLGVLKLAQC
DEQVFTTTNLKLLRQIAERVSIAIDNALAYREIQRLKERLVDENLALTEQLNNVESEFGEIIGRSEAMNNVLKQVEMVAH
SDSTVLILGETGTGKELIARAIHNLSGRNGRRMVKMNCAAMPAGLLESDLFGHERGAFTGASAQRIGRFELADKSSLFLD
EVGDMPLELQPKLLRVLQEQEFERLGSNKLIQTDVRLIAATNRDLKQMVIDREFRSDLYYRLNVFPIHLPPLRERPDDIP
LLVKAFTFKIARRMGRNIDSIPAETLRTLTRMEWPGNVRELENVIERAVLLTRGNVLQLSLPERDIVEAPRTPAVLPEEG
EDEYQLIVRVLKESNGVVAGPKGAAQRLGLKRTTLLSRMKRLGINKDELV

Sequences:

>Translated_690_residues
MSYTPMSDLGQQGLFDITRLLLQQPDLAALSETLTRLVQQSALADEAAIILWNAGNHRAARYACDEAGHPVSYEDETVLA
HGPVRRLLSRPDALHCDHETFADTWPQLIRSGLYRPFGYYSLLPLAADGRIFGGCEFLRRDNRPWSEKEFQRLHTFAQIV
AVVTEQIQNRVSNNVDYDLLCHERDNFRILVAITNAVLSRLDIDELVSEVAKEIHRYFRIDAISVVLRSDRKGKLNIYST
HYLDASHPVHDQSEVDEAGTLTERVFKSKEMLLLNLHEHDTLAPYEKMLFEMWGNKIQTLCLLPLMSGNTLLGVLKLAQC
DEQVFTTTNLKLLRQIAERVSIAIDNALAYREIQRLKERLVDENLALTEQLNNVESEFGEIIGRSEAMNNVLKQVEMVAH
SDSTVLILGETGTGKELIARAIHNLSGRNGRRMVKMNCAAMPAGLLESDLFGHERGAFTGASAQRIGRFELADKSSLFLD
EVGDMPLELQPKLLRVLQEQEFERLGSNKLIQTDVRLIAATNRDLKQMVIDREFRSDLYYRLNVFPIHLPPLRERPDDIP
LLVKAFTFKIARRMGRNIDSIPAETLRTLTRMEWPGNVRELENVIERAVLLTRGNVLQLSLPERDIVEAPRTPAVLPEEG
EDEYQLIVRVLKESNGVVAGPKGAAQRLGLKRTTLLSRMKRLGINKDELV
>Mature_689_residues
SYTPMSDLGQQGLFDITRLLLQQPDLAALSETLTRLVQQSALADEAAIILWNAGNHRAARYACDEAGHPVSYEDETVLAH
GPVRRLLSRPDALHCDHETFADTWPQLIRSGLYRPFGYYSLLPLAADGRIFGGCEFLRRDNRPWSEKEFQRLHTFAQIVA
VVTEQIQNRVSNNVDYDLLCHERDNFRILVAITNAVLSRLDIDELVSEVAKEIHRYFRIDAISVVLRSDRKGKLNIYSTH
YLDASHPVHDQSEVDEAGTLTERVFKSKEMLLLNLHEHDTLAPYEKMLFEMWGNKIQTLCLLPLMSGNTLLGVLKLAQCD
EQVFTTTNLKLLRQIAERVSIAIDNALAYREIQRLKERLVDENLALTEQLNNVESEFGEIIGRSEAMNNVLKQVEMVAHS
DSTVLILGETGTGKELIARAIHNLSGRNGRRMVKMNCAAMPAGLLESDLFGHERGAFTGASAQRIGRFELADKSSLFLDE
VGDMPLELQPKLLRVLQEQEFERLGSNKLIQTDVRLIAATNRDLKQMVIDREFRSDLYYRLNVFPIHLPPLRERPDDIPL
LVKAFTFKIARRMGRNIDSIPAETLRTLTRMEWPGNVRELENVIERAVLLTRGNVLQLSLPERDIVEAPRTPAVLPEEGE
DEYQLIVRVLKESNGVVAGPKGAAQRLGLKRTTLLSRMKRLGINKDELV

Specific function: Required for induction of expression of the formate dehydrogenase H and hydrogenase-3 structural genes [H]

COG id: COG3604

COG function: function code KT; Transcriptional regulator containing GAF, AAA-type ATPase, and DNA binding domains

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1789087, Length=694, Percent_Identity=84.0057636887608, Blast_Score=1190, Evalue=0.0,
Organism=Escherichia coli, GI87082117, Length=572, Percent_Identity=50.5244755244755, Blast_Score=516, Evalue=1e-147,
Organism=Escherichia coli, GI1790437, Length=313, Percent_Identity=46.0063897763578, Blast_Score=263, Evalue=2e-71,
Organism=Escherichia coli, GI1788550, Length=320, Percent_Identity=46.25, Blast_Score=262, Evalue=6e-71,
Organism=Escherichia coli, GI87082152, Length=332, Percent_Identity=41.2650602409639, Blast_Score=241, Evalue=1e-64,
Organism=Escherichia coli, GI1788905, Length=310, Percent_Identity=43.2258064516129, Blast_Score=239, Evalue=4e-64,
Organism=Escherichia coli, GI1790299, Length=371, Percent_Identity=40.7008086253369, Blast_Score=239, Evalue=5e-64,
Organism=Escherichia coli, GI1789233, Length=325, Percent_Identity=40.6153846153846, Blast_Score=236, Evalue=4e-63,
Organism=Escherichia coli, GI87081872, Length=232, Percent_Identity=47.4137931034483, Blast_Score=203, Evalue=3e-53,
Organism=Escherichia coli, GI1786524, Length=332, Percent_Identity=38.5542168674699, Blast_Score=198, Evalue=1e-51,
Organism=Escherichia coli, GI1787583, Length=329, Percent_Identity=36.4741641337386, Blast_Score=189, Evalue=4e-49,
Organism=Escherichia coli, GI1789828, Length=263, Percent_Identity=34.9809885931559, Blast_Score=143, Evalue=4e-35,
Organism=Escherichia coli, GI87081858, Length=331, Percent_Identity=32.02416918429, Blast_Score=141, Evalue=1e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR003018
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078 [H]

Pfam domain/function: PF01590 GAF; PF02954 HTH_8; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 78232; Mature: 78101

Theoretical pI: Translated: 6.19; Mature: 6.19

Prosite motif: PS00675 SIGMA54_INTERACT_1 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSYTPMSDLGQQGLFDITRLLLQQPDLAALSETLTRLVQQSALADEAAIILWNAGNHRAA
CCCCCHHHHCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCHH
RYACDEAGHPVSYEDETVLAHGPVRRLLSRPDALHCDHETFADTWPQLIRSGLYRPFGYY
HHHHHCCCCCCCCCCCEEECHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCHH
SLLPLAADGRIFGGCEFLRRDNRPWSEKEFQRLHTFAQIVAVVTEQIQNRVSNNVDYDLL
HEEEECCCCCEEHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEE
CHERDNFRILVAITNAVLSRLDIDELVSEVAKEIHRYFRIDAISVVLRSDRKGKLNIYST
EECCCCCEEEEEEHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEE
HYLDASHPVHDQSEVDEAGTLTERVFKSKEMLLLNLHEHDTLAPYEKMLFEMWGNKIQTL
EEECCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHCCHHHHH
CLLPLMSGNTLLGVLKLAQCDEQVFTTTNLKLLRQIAERVSIAIDNALAYREIQRLKERL
HHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VDENLALTEQLNNVESEFGEIIGRSEAMNNVLKQVEMVAHSDSTVLILGETGTGKELIAR
HCCCHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHH
AIHNLSGRNGRRMVKMNCAAMPAGLLESDLFGHERGAFTGASAQRIGRFELADKSSLFLD
HHHHCCCCCCCEEEEEEHHHCCCHHHHHHHCCCCCCCCCCCHHHHCCCEEECCCCHHHHH
EVGDMPLELQPKLLRVLQEQEFERLGSNKLIQTDVRLIAATNRDLKQMVIDREFRSDLYY
HHCCCCCCCCHHHHHHHHHHHHHHHCCCCEEHHHHHHEEECCHHHHHHHHHHHHHHCCEE
RLNVFPIHLPPLRERPDDIPLLVKAFTFKIARRMGRNIDSIPAETLRTLTRMEWPGNVRE
EEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCCHHH
LENVIERAVLLTRGNVLQLSLPERDIVEAPRTPAVLPEEGEDEYQLIVRVLKESNGVVAG
HHHHHHHHHHHCCCCEEEEECCCHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHCCCEEEC
PKGAAQRLGLKRTTLLSRMKRLGINKDELV
CCHHHHHCCCHHHHHHHHHHHCCCCHHCCC
>Mature Secondary Structure 
SYTPMSDLGQQGLFDITRLLLQQPDLAALSETLTRLVQQSALADEAAIILWNAGNHRAA
CCCCHHHHCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCHH
RYACDEAGHPVSYEDETVLAHGPVRRLLSRPDALHCDHETFADTWPQLIRSGLYRPFGYY
HHHHHCCCCCCCCCCCEEECHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCHH
SLLPLAADGRIFGGCEFLRRDNRPWSEKEFQRLHTFAQIVAVVTEQIQNRVSNNVDYDLL
HEEEECCCCCEEHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEE
CHERDNFRILVAITNAVLSRLDIDELVSEVAKEIHRYFRIDAISVVLRSDRKGKLNIYST
EECCCCCEEEEEEHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEE
HYLDASHPVHDQSEVDEAGTLTERVFKSKEMLLLNLHEHDTLAPYEKMLFEMWGNKIQTL
EEECCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHCCHHHHH
CLLPLMSGNTLLGVLKLAQCDEQVFTTTNLKLLRQIAERVSIAIDNALAYREIQRLKERL
HHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VDENLALTEQLNNVESEFGEIIGRSEAMNNVLKQVEMVAHSDSTVLILGETGTGKELIAR
HCCCHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHH
AIHNLSGRNGRRMVKMNCAAMPAGLLESDLFGHERGAFTGASAQRIGRFELADKSSLFLD
HHHHCCCCCCCEEEEEEHHHCCCHHHHHHHCCCCCCCCCCCHHHHCCCEEECCCCHHHHH
EVGDMPLELQPKLLRVLQEQEFERLGSNKLIQTDVRLIAATNRDLKQMVIDREFRSDLYY
HHCCCCCCCCHHHHHHHHHHHHHHHCCCCEEHHHHHHEEECCHHHHHHHHHHHHHHCCEE
RLNVFPIHLPPLRERPDDIPLLVKAFTFKIARRMGRNIDSIPAETLRTLTRMEWPGNVRE
EEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCCHHH
LENVIERAVLLTRGNVLQLSLPERDIVEAPRTPAVLPEEGEDEYQLIVRVLKESNGVVAG
HHHHHHHHHHHCCCCEEEEECCCHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHCCCEEEC
PKGAAQRLGLKRTTLLSRMKRLGINKDELV
CCHHHHHCCCHHHHHHHHHHHCCCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2280686; 2118503; 9278503 [H]