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The map label for this gene is gshA [H]

Identifier: 238896143

GI number: 238896143

Start: 4114119

End: 4115675

Strand: Reverse

Name: gshA [H]

Synonym: KP1_4285

Alternate gene names: 238896143

Gene position: 4115675-4114119 (Counterclockwise)

Preceding gene: 238896144

Following gene: 238896142

Centisome position: 78.42

GC content: 57.42

Gene sequence:

>1557_bases
TTGATCCCGGACGTATCACAGGCGCTGGCCTGGCTGGAAAAACATCCGCAGGCTCTGCAGGGTATTCAACGTGGTCTGGA
GCGGGAAACGCTGCGCGTGAACGCAGACGGCACCCTTGCAACGACCGGTCACCCTCCGGCGTTAGGCTCAGCGCTGACGC
ATAAATGGATTACCACCGATTTTGCCGAAGCTTTACTTGAGTTCATTACGCCAGTAGATGGCGATATTGAGCATATGCTG
ACCTTTATGCGCGATGTCCATCGCTATACCGCGCGTCAGCTTGGCGACGAGCGGATGTGGCCGCTGAGTATGCCATGCTA
TATCGCCCCCGGTCAGGATATTGAGCTGGCGCAGTACGGCACCTCTAACGTGGGGCGCCTGAAAACGCTATATCGCGAGG
GGCTGAAGAACCGCTATGGCGCGCTGATGCAGACTATCTCCGGCGTGCACTATAACTTCTCCCTACCGATGGCCTTCTGG
CAGGCAAAATGTGGTGTGGAAGATGCCGAGAGTGGTAAAGAGGCCATTTCGGCGGGCTATTTCCGCTCAATTCGTAACTA
CTATCGCTTTGGCTGGGTGATCCCCTATCTGTTTGGCGCCTCGCCGGCGATTTGCTCCTCATTCCTGCAAGGCAAACCGA
CAACGCTGCCCTTTGAGGAAACGGGCAACGGCATGTATTACCTGCCGTATGCCACCTCGCTGCGCCTCAGCGACCTTGGT
TATACCAATAAGTCGCAAAGCAATCTCGGAATTACGTTTAACGATCTGCACGAGTATGTGGCAGGATTGAAGCGGGCGAT
TAAAACGCCGTCGGAAGAGTACGCCAAAATCGGGCTGCAGAAAGACGGCAAGTATCTGCAGATCAACAGCAACATTCTGC
AGATTGAAAATGAACTTTATGCGCCGATCCGCCCGAAACGCGTCACCCGTCGGGGCGAAACCCCGTCCGATGCGCTGTTG
CGCGGTGGTATTGAGTATATCGAAGTGCGTTCGCTGGATATCAATCCGTTCTCGCCGATCGGCGTTGACGCCCAGCAGGT
GCGTTTCCTCGACCTGTTTATGGTCTGGTGCGCGCTGGCCGACGCGCCGGAGATGAGCAGCGATGAGCTGCTGTGCACCC
GCACCAACTGGAATCGGGTTATTCTGGAAGGGCGTAAGCCGGGCTTAACGCTGGGGATTGGCTGCGAAAGCGCGCAGTTC
CCGCTGGCTCAGGTGGGCAAAGATCTGTTCCGCGATTTACGCCGCGTGGCTCAGACCCTGGACAGTATCCACGGTGGCCA
GGCATATCAGCAGGTCTGCGACGAGCTGCTGGCCTGCTTTGACGATCCGGAATTAACCTTCTCAGCCCGCATTCTGCGTT
CTATGATTGAAGAAGGCATTGGCGGTACCGGCCGTGCGCTTGCCGATCGTTACCGGACGCAGCTGCGCGAAGAGCCGCTG
GAGATCCTGAGCGAAGACGATTTTATCGCCGAGCGCGACGCGTCGGTGGCGCGACAGAAGAAAGTGGAAGCTGAAGACAG
CGAGCCGTTTGAGGCGCTGCTGGCGCGGCACGCGTAG

Upstream 100 bases:

>100_bases
AGCGACGCTTCAGGGCATGACGTGGTGGCACTAATTGGACTGACAGTTCATCCATTACAATTATGCTTATTCAAACGATT
TCGACAGGCGGGAGGTCAAA

Downstream 100 bases:

>100_bases
CGCTGGAAAAGAAAAAGGCCACATCACTGTGGCCAAACTTTCATCTCTGATTCAGGGATGATGATGATAATAAATGCGCG
TCTTTCATATACTGAGACTC

Product: glutamate--cysteine ligase

Products: NA

Alternate protein names: Gamma-ECS; GCS; Gamma-glutamylcysteine synthetase [H]

Number of amino acids: Translated: 518; Mature: 518

Protein sequence:

>518_residues
MIPDVSQALAWLEKHPQALQGIQRGLERETLRVNADGTLATTGHPPALGSALTHKWITTDFAEALLEFITPVDGDIEHML
TFMRDVHRYTARQLGDERMWPLSMPCYIAPGQDIELAQYGTSNVGRLKTLYREGLKNRYGALMQTISGVHYNFSLPMAFW
QAKCGVEDAESGKEAISAGYFRSIRNYYRFGWVIPYLFGASPAICSSFLQGKPTTLPFEETGNGMYYLPYATSLRLSDLG
YTNKSQSNLGITFNDLHEYVAGLKRAIKTPSEEYAKIGLQKDGKYLQINSNILQIENELYAPIRPKRVTRRGETPSDALL
RGGIEYIEVRSLDINPFSPIGVDAQQVRFLDLFMVWCALADAPEMSSDELLCTRTNWNRVILEGRKPGLTLGIGCESAQF
PLAQVGKDLFRDLRRVAQTLDSIHGGQAYQQVCDELLACFDDPELTFSARILRSMIEEGIGGTGRALADRYRTQLREEPL
EILSEDDFIAERDASVARQKKVEAEDSEPFEALLARHA

Sequences:

>Translated_518_residues
MIPDVSQALAWLEKHPQALQGIQRGLERETLRVNADGTLATTGHPPALGSALTHKWITTDFAEALLEFITPVDGDIEHML
TFMRDVHRYTARQLGDERMWPLSMPCYIAPGQDIELAQYGTSNVGRLKTLYREGLKNRYGALMQTISGVHYNFSLPMAFW
QAKCGVEDAESGKEAISAGYFRSIRNYYRFGWVIPYLFGASPAICSSFLQGKPTTLPFEETGNGMYYLPYATSLRLSDLG
YTNKSQSNLGITFNDLHEYVAGLKRAIKTPSEEYAKIGLQKDGKYLQINSNILQIENELYAPIRPKRVTRRGETPSDALL
RGGIEYIEVRSLDINPFSPIGVDAQQVRFLDLFMVWCALADAPEMSSDELLCTRTNWNRVILEGRKPGLTLGIGCESAQF
PLAQVGKDLFRDLRRVAQTLDSIHGGQAYQQVCDELLACFDDPELTFSARILRSMIEEGIGGTGRALADRYRTQLREEPL
EILSEDDFIAERDASVARQKKVEAEDSEPFEALLARHA
>Mature_518_residues
MIPDVSQALAWLEKHPQALQGIQRGLERETLRVNADGTLATTGHPPALGSALTHKWITTDFAEALLEFITPVDGDIEHML
TFMRDVHRYTARQLGDERMWPLSMPCYIAPGQDIELAQYGTSNVGRLKTLYREGLKNRYGALMQTISGVHYNFSLPMAFW
QAKCGVEDAESGKEAISAGYFRSIRNYYRFGWVIPYLFGASPAICSSFLQGKPTTLPFEETGNGMYYLPYATSLRLSDLG
YTNKSQSNLGITFNDLHEYVAGLKRAIKTPSEEYAKIGLQKDGKYLQINSNILQIENELYAPIRPKRVTRRGETPSDALL
RGGIEYIEVRSLDINPFSPIGVDAQQVRFLDLFMVWCALADAPEMSSDELLCTRTNWNRVILEGRKPGLTLGIGCESAQF
PLAQVGKDLFRDLRRVAQTLDSIHGGQAYQQVCDELLACFDDPELTFSARILRSMIEEGIGGTGRALADRYRTQLREEPL
EILSEDDFIAERDASVARQKKVEAEDSEPFEALLARHA

Specific function: Glutathione biosynthesis; first step. [C]

COG id: COG2918

COG function: function code H; Gamma-glutamylcysteine synthetase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamate--cysteine ligase type 1 family. Type 1 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789044, Length=518, Percent_Identity=87.2586872586873, Blast_Score=951, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007370
- InterPro:   IPR006334 [H]

Pfam domain/function: PF04262 Glu_cys_ligase [H]

EC number: =6.3.2.2 [H]

Molecular weight: Translated: 58102; Mature: 58102

Theoretical pI: Translated: 5.18; Mature: 5.18

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIPDVSQALAWLEKHPQALQGIQRGLERETLRVNADGTLATTGHPPALGSALTHKWITTD
CCCCHHHHHHHHHHCHHHHHHHHHCCCCCEEEECCCCEEEECCCCCHHHHHHHHHHHHHH
FAEALLEFITPVDGDIEHMLTFMRDVHRYTARQLGDERMWPLSMPCYIAPGQDIELAQYG
HHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEECCCCCCCHHHCC
TSNVGRLKTLYREGLKNRYGALMQTISGVHYNFSLPMAFWQAKCGVEDAESGKEAISAGY
CCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECHHHHHHHHCCCCCHHHHHHHHHHHH
FRSIRNYYRFGWVIPYLFGASPAICSSFLQGKPTTLPFEETGNGMYYLPYATSLRLSDLG
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCHHHCCCCEEEECCCCEEEEHHCC
YTNKSQSNLGITFNDLHEYVAGLKRAIKTPSEEYAKIGLQKDGKYLQINSNILQIENELY
CCCCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHCCCCCCCCEEEECCCEEEECHHHC
APIRPKRVTRRGETPSDALLRGGIEYIEVRSLDINPFSPIGVDAQQVRFLDLFMVWCALA
CCCCCHHHHCCCCCCHHHHHHCCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHC
DAPEMSSDELLCTRTNWNRVILEGRKPGLTLGIGCESAQFPLAQVGKDLFRDLRRVAQTL
CCCCCCCCCEEEEECCCCEEEEECCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHH
DSIHGGQAYQQVCDELLACFDDPELTFSARILRSMIEEGIGGTGRALADRYRTQLREEPL
HHHCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHH
EILSEDDFIAERDASVARQKKVEAEDSEPFEALLARHA
HHHCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCC
>Mature Secondary Structure
MIPDVSQALAWLEKHPQALQGIQRGLERETLRVNADGTLATTGHPPALGSALTHKWITTD
CCCCHHHHHHHHHHCHHHHHHHHHCCCCCEEEECCCCEEEECCCCCHHHHHHHHHHHHHH
FAEALLEFITPVDGDIEHMLTFMRDVHRYTARQLGDERMWPLSMPCYIAPGQDIELAQYG
HHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEECCCCCCCHHHCC
TSNVGRLKTLYREGLKNRYGALMQTISGVHYNFSLPMAFWQAKCGVEDAESGKEAISAGY
CCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECHHHHHHHHCCCCCHHHHHHHHHHHH
FRSIRNYYRFGWVIPYLFGASPAICSSFLQGKPTTLPFEETGNGMYYLPYATSLRLSDLG
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCHHHCCCCEEEECCCCEEEEHHCC
YTNKSQSNLGITFNDLHEYVAGLKRAIKTPSEEYAKIGLQKDGKYLQINSNILQIENELY
CCCCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHCCCCCCCCEEEECCCEEEECHHHC
APIRPKRVTRRGETPSDALLRGGIEYIEVRSLDINPFSPIGVDAQQVRFLDLFMVWCALA
CCCCCHHHHCCCCCCHHHHHHCCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHC
DAPEMSSDELLCTRTNWNRVILEGRKPGLTLGIGCESAQFPLAQVGKDLFRDLRRVAQTL
CCCCCCCCCEEEEECCCCEEEEECCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHH
DSIHGGQAYQQVCDELLACFDDPELTFSARILRSMIEEGIGGTGRALADRYRTQLREEPL
HHHCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHH
EILSEDDFIAERDASVARQKKVEAEDSEPFEALLARHA
HHHCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA