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The map label for this gene is pdxB [H]

Identifier: 238895840

GI number: 238895840

Start: 3783327

End: 3784463

Strand: Reverse

Name: pdxB [H]

Synonym: KP1_3959

Alternate gene names: 238895840

Gene position: 3784463-3783327 (Counterclockwise)

Preceding gene: 238895841

Following gene: 238895839

Centisome position: 72.11

GC content: 60.25

Gene sequence:

>1137_bases
GTGAAAATCCTCGTTGATGAAAATATGCCCTATGCCCGTGAGCTTTTTAGCCGTTTAGGCGACGTGCAGGCGATCCCAGG
CCGTCCGGTCCCGGCCGATGCCCTGACCGATGCGGATGCCCTAATGGTGCGCTCGGTCACCCGGGTCAATGAAGCATTGC
TCGCCGGCAAGGCCATTAAGTTCGTCGGCACCGCTACCGCCGGCACCGATCACGTCGATCAGGCGTGGCTGCAGCAGGCG
GGGATTGGTTTCTCTGCGGCGCCCGGCTGTAACGCCATTGCGGTGGTAGAGTACGTCTTTTCATCGCTGCTGATGCTGGC
CGAACGCGACGGCTTTGCACTGCGCGATCGCACCGTCGGGATCGTCGGCGTCGGTAACGTTGGCGGGCGACTGCAAAAGC
GCCTGGAAGCGTTGGGGATCAAAACTCTGCTCTGCGACCCACCGCGCGCCGATCGCGGCGACGAAGGTGACTTCCGCTCC
CTCGAGGCGCTGGTCCAGGAAGCCGATGTCATCACCTTCCATACGCCGCTGTATAAAGAAGGGCAATATAAAACCCTGCA
TCTGGCGGATGAAGCGCTGATAAGCCGCCTGAAGCCTGGAACGATTTTGATCAACGCCTGCCGCGGGCCGGTGGTCGATA
ACGCCGCACTGCTAAAGCGGCTGGAAGCTGGTCAGCCGCTGAGTGTGGTATTGGACGTCTGGGAGCCGGAGCCGGATCTA
AACGTTGAACTTCTCAAGCGAGTGGATATCGGTACCGCGCATATCGCCGGCTACACTCTCGAAGGGAAAGCGCGCGGGAC
GACGCAGGTTTTTGAAGCCTACAGCGCCTTTATCGGCCATCCGCAGCAGGTAGCGCTGGACACTCTGCTGCCGGCGCCGG
AATTTGGCCGCATTACCCTGCATGGACCGCTCGATCAGCCGACGCTGAAAAGACTGGTCCACTTGGTGTATGATGTGCGC
CGCGATGATGCGCCGCTGCGGAAGGTGGCCGGCGTCGCCGGTGAATTCGACAAGCTGCGTAAAAATTATCAGGAACGTCG
CGAATGGTCTTCGCTGTATGTGCAGTGCAGCGATGAGCAGGCGGCGACGCTGTTGCGCCAGCTCGGTTTTAACGCCGTGC
ATCATCCTGTTCGTTAA

Upstream 100 bases:

>100_bases
TTCGATATCGCGGTGCTGGAACCAGGTGATGAGTATGCCTGGCGGCCAGATTATGGCACACTTCCCCAGTTAACTCTCGT
TCTCAGACAGGTACTGAACT

Downstream 100 bases:

>100_bases
TGTCTTCTTAATGCCCCTGCCGGATACTCCGGCGGGGCCGCTTTATTTCTGGAGTTAACCACCATGTCTGAAGGCTGGAA
TATTGCCGTACTGGGCGCAA

Product: erythronate-4-phosphate dehydrogenase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 378; Mature: 378

Protein sequence:

>378_residues
MKILVDENMPYARELFSRLGDVQAIPGRPVPADALTDADALMVRSVTRVNEALLAGKAIKFVGTATAGTDHVDQAWLQQA
GIGFSAAPGCNAIAVVEYVFSSLLMLAERDGFALRDRTVGIVGVGNVGGRLQKRLEALGIKTLLCDPPRADRGDEGDFRS
LEALVQEADVITFHTPLYKEGQYKTLHLADEALISRLKPGTILINACRGPVVDNAALLKRLEAGQPLSVVLDVWEPEPDL
NVELLKRVDIGTAHIAGYTLEGKARGTTQVFEAYSAFIGHPQQVALDTLLPAPEFGRITLHGPLDQPTLKRLVHLVYDVR
RDDAPLRKVAGVAGEFDKLRKNYQERREWSSLYVQCSDEQAATLLRQLGFNAVHHPVR

Sequences:

>Translated_378_residues
MKILVDENMPYARELFSRLGDVQAIPGRPVPADALTDADALMVRSVTRVNEALLAGKAIKFVGTATAGTDHVDQAWLQQA
GIGFSAAPGCNAIAVVEYVFSSLLMLAERDGFALRDRTVGIVGVGNVGGRLQKRLEALGIKTLLCDPPRADRGDEGDFRS
LEALVQEADVITFHTPLYKEGQYKTLHLADEALISRLKPGTILINACRGPVVDNAALLKRLEAGQPLSVVLDVWEPEPDL
NVELLKRVDIGTAHIAGYTLEGKARGTTQVFEAYSAFIGHPQQVALDTLLPAPEFGRITLHGPLDQPTLKRLVHLVYDVR
RDDAPLRKVAGVAGEFDKLRKNYQERREWSSLYVQCSDEQAATLLRQLGFNAVHHPVR
>Mature_378_residues
MKILVDENMPYARELFSRLGDVQAIPGRPVPADALTDADALMVRSVTRVNEALLAGKAIKFVGTATAGTDHVDQAWLQQA
GIGFSAAPGCNAIAVVEYVFSSLLMLAERDGFALRDRTVGIVGVGNVGGRLQKRLEALGIKTLLCDPPRADRGDEGDFRS
LEALVQEADVITFHTPLYKEGQYKTLHLADEALISRLKPGTILINACRGPVVDNAALLKRLEAGQPLSVVLDVWEPEPDL
NVELLKRVDIGTAHIAGYTLEGKARGTTQVFEAYSAFIGHPQQVALDTLLPAPEFGRITLHGPLDQPTLKRLVHLVYDVR
RDDAPLRKVAGVAGEFDKLRKNYQERREWSSLYVQCSDEQAATLLRQLGFNAVHHPVR

Specific function: Catalyzes the oxidation of erythronate-4-phosphate to 3- hydroxy-2-oxo-4-phosphonooxybutanoate [H]

COG id: COG0111

COG function: function code HE; Phosphoglycerate dehydrogenase and related dehydrogenases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family. PdxB subfamily [H]

Homologues:

Organism=Homo sapiens, GI23308577, Length=292, Percent_Identity=27.7397260273973, Blast_Score=99, Evalue=8e-21,
Organism=Homo sapiens, GI61743967, Length=228, Percent_Identity=25.8771929824561, Blast_Score=72, Evalue=7e-13,
Organism=Homo sapiens, GI4557497, Length=228, Percent_Identity=25.8771929824561, Blast_Score=72, Evalue=7e-13,
Organism=Homo sapiens, GI145580578, Length=229, Percent_Identity=24.8908296943231, Blast_Score=70, Evalue=3e-12,
Organism=Homo sapiens, GI4557499, Length=229, Percent_Identity=24.8908296943231, Blast_Score=70, Evalue=3e-12,
Organism=Homo sapiens, GI6912396, Length=273, Percent_Identity=26.007326007326, Blast_Score=70, Evalue=4e-12,
Organism=Homo sapiens, GI145580575, Length=276, Percent_Identity=25, Blast_Score=68, Evalue=2e-11,
Organism=Escherichia coli, GI1788660, Length=378, Percent_Identity=85.4497354497355, Blast_Score=639, Evalue=0.0,
Organism=Escherichia coli, GI1787645, Length=210, Percent_Identity=27.6190476190476, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI1789279, Length=257, Percent_Identity=29.9610894941634, Blast_Score=75, Evalue=6e-15,
Organism=Escherichia coli, GI87082289, Length=159, Percent_Identity=32.7044025157233, Blast_Score=69, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI17532191, Length=256, Percent_Identity=29.296875, Blast_Score=99, Evalue=4e-21,
Organism=Saccharomyces cerevisiae, GI6322116, Length=323, Percent_Identity=27.2445820433437, Blast_Score=93, Evalue=6e-20,
Organism=Saccharomyces cerevisiae, GI6320925, Length=389, Percent_Identity=25.4498714652956, Blast_Score=92, Evalue=2e-19,
Organism=Saccharomyces cerevisiae, GI6324964, Length=276, Percent_Identity=26.0869565217391, Blast_Score=81, Evalue=3e-16,
Organism=Saccharomyces cerevisiae, GI6324055, Length=174, Percent_Identity=27.5862068965517, Blast_Score=79, Evalue=1e-15,
Organism=Drosophila melanogaster, GI19921140, Length=268, Percent_Identity=27.2388059701493, Blast_Score=80, Evalue=2e-15,
Organism=Drosophila melanogaster, GI28574286, Length=210, Percent_Identity=28.5714285714286, Blast_Score=79, Evalue=7e-15,
Organism=Drosophila melanogaster, GI24646446, Length=228, Percent_Identity=26.7543859649123, Blast_Score=78, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24646448, Length=228, Percent_Identity=26.7543859649123, Blast_Score=78, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24646452, Length=228, Percent_Identity=26.7543859649123, Blast_Score=78, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24646450, Length=228, Percent_Identity=26.7543859649123, Blast_Score=78, Evalue=1e-14,
Organism=Drosophila melanogaster, GI62472511, Length=228, Percent_Identity=26.7543859649123, Blast_Score=77, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24585514, Length=210, Percent_Identity=26.6666666666667, Blast_Score=70, Evalue=3e-12,
Organism=Drosophila melanogaster, GI28574282, Length=210, Percent_Identity=26.6666666666667, Blast_Score=70, Evalue=3e-12,
Organism=Drosophila melanogaster, GI45551003, Length=210, Percent_Identity=26.6666666666667, Blast_Score=70, Evalue=3e-12,
Organism=Drosophila melanogaster, GI28574284, Length=210, Percent_Identity=26.6666666666667, Blast_Score=70, Evalue=3e-12,
Organism=Drosophila melanogaster, GI45552429, Length=210, Percent_Identity=26.6666666666667, Blast_Score=69, Evalue=4e-12,
Organism=Drosophila melanogaster, GI24585516, Length=251, Percent_Identity=26.2948207171315, Blast_Score=67, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006139
- InterPro:   IPR006140
- InterPro:   IPR020921
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00389 2-Hacid_dh; PF02826 2-Hacid_dh_C [H]

EC number: =1.1.1.290 [H]

Molecular weight: Translated: 41351; Mature: 41351

Theoretical pI: Translated: 6.43; Mature: 6.43

Prosite motif: PS00065 D_2_HYDROXYACID_DH_1 ; PS00671 D_2_HYDROXYACID_DH_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKILVDENMPYARELFSRLGDVQAIPGRPVPADALTDADALMVRSVTRVNEALLAGKAIK
CEEEECCCCCHHHHHHHHHCCCCCCCCCCCCHHHHCCHHHHHHHHHHHHHHHHHHCCCEE
FVGTATAGTDHVDQAWLQQAGIGFSAAPGCNAIAVVEYVFSSLLMLAERDGFALRDRTVG
EEEECCCCCHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEECCEEE
IVGVGNVGGRLQKRLEALGIKTLLCDPPRADRGDEGDFRSLEALVQEADVITFHTPLYKE
EEEECCCCHHHHHHHHHHCCHHEECCCCCCCCCCCCCHHHHHHHHHHCCEEEEECCCCCC
GQYKTLHLADEALISRLKPGTILINACRGPVVDNAALLKRLEAGQPLSVVLDVWEPEPDL
CCEEEEEHHHHHHHHHCCCCEEEEECCCCCCCCCHHHHHHHHCCCCCEEEEEECCCCCCC
NVELLKRVDIGTAHIAGYTLEGKARGTTQVFEAYSAFIGHPQQVALDTLLPAPEFGRITL
CHHHHHHHCCCCCCEEEEEECCCCCCHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCEEEE
HGPLDQPTLKRLVHLVYDVRRDDAPLRKVAGVAGEFDKLRKNYQERREWSSLYVQCSDEQ
ECCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHEEEECCHH
AATLLRQLGFNAVHHPVR
HHHHHHHHCCHHHCCCCC
>Mature Secondary Structure
MKILVDENMPYARELFSRLGDVQAIPGRPVPADALTDADALMVRSVTRVNEALLAGKAIK
CEEEECCCCCHHHHHHHHHCCCCCCCCCCCCHHHHCCHHHHHHHHHHHHHHHHHHCCCEE
FVGTATAGTDHVDQAWLQQAGIGFSAAPGCNAIAVVEYVFSSLLMLAERDGFALRDRTVG
EEEECCCCCHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEECCEEE
IVGVGNVGGRLQKRLEALGIKTLLCDPPRADRGDEGDFRSLEALVQEADVITFHTPLYKE
EEEECCCCHHHHHHHHHHCCHHEECCCCCCCCCCCCCHHHHHHHHHHCCEEEEECCCCCC
GQYKTLHLADEALISRLKPGTILINACRGPVVDNAALLKRLEAGQPLSVVLDVWEPEPDL
CCEEEEEHHHHHHHHHCCCCEEEEECCCCCCCCCHHHHHHHHCCCCCEEEEEECCCCCCC
NVELLKRVDIGTAHIAGYTLEGKARGTTQVFEAYSAFIGHPQQVALDTLLPAPEFGRITL
CHHHHHHHCCCCCCEEEEEECCCCCCHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCEEEE
HGPLDQPTLKRLVHLVYDVRRDDAPLRKVAGVAGEFDKLRKNYQERREWSSLYVQCSDEQ
ECCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHEEEECCHH
AATLLRQLGFNAVHHPVR
HHHHHHHHCCHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA