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The map label for this gene is bcr [H]

Identifier: 238895742

GI number: 238895742

Start: 3675967

End: 3677163

Strand: Reverse

Name: bcr [H]

Synonym: KP1_3845

Alternate gene names: 238895742

Gene position: 3677163-3675967 (Counterclockwise)

Preceding gene: 238895743

Following gene: 238895741

Centisome position: 70.06

GC content: 59.48

Gene sequence:

>1197_bases
GTGACGCTGAAGCAAAATTCTTCACTGGGGATTGTGTTTATCCTTGGCCTGCTGGCCATGCTGATGCCGCTGTCTATTGA
TATGTATCTTCCGGCCCTGCCGGTGATCGCCGCCCAGTATAACGTCCCGGACGGCAGCGCGCAGATGACGCTCAGCACCT
ATATCCTCGGCTTTGCGCTGGGTCAGCTGCTGTACGGCCCGATGGCGGACAGCCTTGGCCGCAAGCCGGTGATCCTCGGC
GGGACGCTGGTCTTCGCGGCGGCGGCGGTAGCCTGCGCCCTGTCGCAAACCGTCGATATGCTGATTGTCATGCGTTTCTT
CCACGGTCTGGCAGCCGCGGCGGCCAGCGTGGTGATTAACGCCCTGATGCGCGACATCTATCCGAAAGATGAGTTTTCGC
GCATGATGTCGTTTGTCATGCTGGTCACGACCATTGCGCCGCTGGTGGCGCCGATGGTCGGCGGGGCGGTGTTGGTCTGG
TTCAGCTGGCATGCCATCTTTTGGATCCTCGCCCTGGTGGCCCTGCTGGCCTCGCTGATGATCGGCCTGTTTGTCCGCGA
AACGCTGCCGGCTGAAAGGCGTCAGCCGTTCCATCTGCGCACCACGCTGGGTAACTTTGCCACGCTGTTCCGCCACAAGC
GGGTACTGAGCTATATGCTGGCGAGCGGCTTTAGCTTCGCGGGCATGTTCTCTTTCCTTAGCGCCGGTCCCTTTGTCTAC
ATCAATATAAACCACGTCGCGCCTCAGCACTTTGGCTACTATTTTGCGCTGAACATCGTTTTTCTGTTTCTGATGACCAT
GTTCAACAGCCGGTTTGTGCGGCGGGTAGGGGCGTTGCGTATGTTCCGCGCCGGGCTGTGGATCCAGTTTGCCATGGCGG
TGTGGATGGTGGTCTGCGCGCTGCTGGATGTCGGCTTTTGGTCACTGGTGATCGGCGTAGCGGCCTTTGTCGGCTGCGTA
TCCATGGTGTCGTCTAACGCCATGGCGGTGATCCTCGATGAGTTTCCGCATATGGCGGGCACTGCTTCGTCGCTGGCGGG
GACTTTCCGTTTCGGTATCGGGGCGATTATCGGCGCGCTGCTGTCGATGGCGACGTTTACCACCGCCTGGCCGATGCTGA
TCTCTATTGCCTTCTGCGCAACCTGCTCTATTTTCTTCTCGCTTTACGCATCCCGGCGGCGAAAAATCGCACGCTAG

Upstream 100 bases:

>100_bases
GCGACATCCTGCTGGATGAATCGCTGGCGCCGGGTGAATATCGTCCGCTGACGGAAGCGGAAATCGCCAGCGTGGGCGCC
CCGCAGCCACGGAGTAAAAC

Downstream 100 bases:

>100_bases
GTCACATTTTCAGGGGCGAAATCGCCCCTTTCTTGATGCATGTCAACCGCAATGGGCTTCAGGGTTCTGCGATTTGTTTC
CTTTATGTAAAATCAATTAG

Product: bicyclomycin/multidrug efflux system

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: Sulfonamide resistance protein [H]

Number of amino acids: Translated: 398; Mature: 397

Protein sequence:

>398_residues
MTLKQNSSLGIVFILGLLAMLMPLSIDMYLPALPVIAAQYNVPDGSAQMTLSTYILGFALGQLLYGPMADSLGRKPVILG
GTLVFAAAAVACALSQTVDMLIVMRFFHGLAAAAASVVINALMRDIYPKDEFSRMMSFVMLVTTIAPLVAPMVGGAVLVW
FSWHAIFWILALVALLASLMIGLFVRETLPAERRQPFHLRTTLGNFATLFRHKRVLSYMLASGFSFAGMFSFLSAGPFVY
ININHVAPQHFGYYFALNIVFLFLMTMFNSRFVRRVGALRMFRAGLWIQFAMAVWMVVCALLDVGFWSLVIGVAAFVGCV
SMVSSNAMAVILDEFPHMAGTASSLAGTFRFGIGAIIGALLSMATFTTAWPMLISIAFCATCSIFFSLYASRRRKIAR

Sequences:

>Translated_398_residues
MTLKQNSSLGIVFILGLLAMLMPLSIDMYLPALPVIAAQYNVPDGSAQMTLSTYILGFALGQLLYGPMADSLGRKPVILG
GTLVFAAAAVACALSQTVDMLIVMRFFHGLAAAAASVVINALMRDIYPKDEFSRMMSFVMLVTTIAPLVAPMVGGAVLVW
FSWHAIFWILALVALLASLMIGLFVRETLPAERRQPFHLRTTLGNFATLFRHKRVLSYMLASGFSFAGMFSFLSAGPFVY
ININHVAPQHFGYYFALNIVFLFLMTMFNSRFVRRVGALRMFRAGLWIQFAMAVWMVVCALLDVGFWSLVIGVAAFVGCV
SMVSSNAMAVILDEFPHMAGTASSLAGTFRFGIGAIIGALLSMATFTTAWPMLISIAFCATCSIFFSLYASRRRKIAR
>Mature_397_residues
TLKQNSSLGIVFILGLLAMLMPLSIDMYLPALPVIAAQYNVPDGSAQMTLSTYILGFALGQLLYGPMADSLGRKPVILGG
TLVFAAAAVACALSQTVDMLIVMRFFHGLAAAAASVVINALMRDIYPKDEFSRMMSFVMLVTTIAPLVAPMVGGAVLVWF
SWHAIFWILALVALLASLMIGLFVRETLPAERRQPFHLRTTLGNFATLFRHKRVLSYMLASGFSFAGMFSFLSAGPFVYI
NINHVAPQHFGYYFALNIVFLFLMTMFNSRFVRRVGALRMFRAGLWIQFAMAVWMVVCALLDVGFWSLVIGVAAFVGCVS
MVSSNAMAVILDEFPHMAGTASSLAGTFRFGIGAIIGALLSMATFTTAWPMLISIAFCATCSIFFSLYASRRRKIAR

Specific function: Involved in sulfonamide (sulfathiazole) and bicyclomycin resistance. Probable membrane translocase [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Bcr/CmlA family [H]

Homologues:

Organism=Escherichia coli, GI1788509, Length=395, Percent_Identity=84.5569620253165, Blast_Score=644, Evalue=0.0,
Organism=Escherichia coli, GI48994889, Length=371, Percent_Identity=29.3800539083558, Blast_Score=132, Evalue=3e-32,
Organism=Escherichia coli, GI1790146, Length=399, Percent_Identity=28.3208020050125, Blast_Score=121, Evalue=1e-28,
Organism=Escherichia coli, GI87082312, Length=348, Percent_Identity=25, Blast_Score=98, Evalue=8e-22,
Organism=Escherichia coli, GI1790794, Length=284, Percent_Identity=21.830985915493, Blast_Score=83, Evalue=3e-17,
Organism=Escherichia coli, GI1787065, Length=250, Percent_Identity=23.6, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI87081738, Length=361, Percent_Identity=25.7617728531856, Blast_Score=77, Evalue=3e-15,
Organism=Escherichia coli, GI1790195, Length=195, Percent_Identity=27.6923076923077, Blast_Score=72, Evalue=5e-14,
Organism=Saccharomyces cerevisiae, GI6319480, Length=266, Percent_Identity=22.9323308270677, Blast_Score=71, Evalue=3e-13,
Organism=Saccharomyces cerevisiae, GI6324264, Length=163, Percent_Identity=23.9263803680982, Blast_Score=67, Evalue=4e-12,
Organism=Saccharomyces cerevisiae, GI6319657, Length=169, Percent_Identity=24.8520710059172, Blast_Score=67, Evalue=7e-12,
Organism=Saccharomyces cerevisiae, GI6322070, Length=171, Percent_Identity=25.7309941520468, Blast_Score=63, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004812
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR004734 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 43496; Mature: 43365

Theoretical pI: Translated: 10.26; Mature: 10.26

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
6.5 %Met     (Translated Protein)
7.8 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
6.3 %Met     (Mature Protein)
7.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TLKQNSSLGIVFILGLLAMLMPLSIDMYLPALPVIAAQYNVPDGSAQMTLSTYILGFAL
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHH
GQLLYGPMADSLGRKPVILGGTLVFAAAAVACALSQTVDMLIVMRFFHGLAAAAASVVIN
HHHHHHHHHHHCCCCCEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ALMRDIYPKDEFSRMMSFVMLVTTIAPLVAPMVGGAVLVWFSWHAIFWILALVALLASLM
HHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IGLFVRETLPAERRQPFHLRTTLGNFATLFRHKRVLSYMLASGFSFAGMFSFLSAGPFVY
HHHHHHHHCCHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCEEE
ININHVAPQHFGYYFALNIVFLFLMTMFNSRFVRRVGALRMFRAGLWIQFAMAVWMVVCA
EEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LLDVGFWSLVIGVAAFVGCVSMVSSNAMAVILDEFPHMAGTASSLAGTFRFGIGAIIGAL
HHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEHHHCCCCCCCHHHHHHHHHHHHHHHHHHH
LSMATFTTAWPMLISIAFCATCSIFFSLYASRRRKIAR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8486276; 9278503; 2694948 [H]