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The map label for this gene is yeiO [H]

Identifier: 238895727

GI number: 238895727

Start: 3659967

End: 3661148

Strand: Direct

Name: yeiO [H]

Synonym: KP1_3830

Alternate gene names: 238895727

Gene position: 3659967-3661148 (Clockwise)

Preceding gene: 238895723

Following gene: 238895729

Centisome position: 69.73

GC content: 60.32

Gene sequence:

>1182_bases
ATGGAAAACCGTAGCGCCGCTCTCCCCCGACGCGGGTTCGATGTCACCTCTTCCGCCTTCTTAATCGTCGCCTTTCTGAC
GGGCATTGCCGGGGCGCTGCAAACCCCGACCCTGAGCCTGTTTCTGACCAACGAAGTGCACGTCCGCCCGGCGATGGTCG
GTTTCTTTTTTACCGGCAGCGCGGTTATTGGCATTCTGGTCAGTCAGTTCCTCGCCGGCCGCTCCGACCGTCAGGGCGAT
CGCAAGCAGCTGATTGTCGTCTGCTGTCTGCTGGGGGTGCTGGCCTGCGTCCTCTTTGCCTGGAACCGCAACTATTTCAT
TTTGCTGTTCATCGGCGTTTTTCTCAGCAGCTTTGGATCGACCGCCAACCCGCAGATGTTTGCCCTCGCGCGGGAGCATG
CCGACAGGACCGGCCGCGAAGCAGTGATGTTCAGCTCCATTTTACGTGCCCAGGTCTCCCTGGCATGGGTGATTGGTCCC
CCGCTGGCCTACGCCCTGGCGATGGGGTTTGGTTTTACCGCGATGTATCTCAGCGCCGCGGCGGCGTTTATCGTCTGCGG
GATCATGGTCTGGCTATTCTTGCCCAGCATGCGCAAAGAAAACCCGGTAGCGACCGGCCGGCTGGAAGCTCCGCGCACGC
ATCGCCGCGATGCGCTGCTGCTGTTCAGCATCTGCACCATGATGTGGGGTACTAACAGCCTGTACATCATTAACATGCCG
CTGTTTATCATTAACGAGCTGCATCTGCCGGAGAAGCTGGCGGGGCTGATGATGGGCACCGCCGCCGGACTGGAAATTCC
GACCATGCTGATTGCCGGTTACTACGCCCGGCGTTTCGGCAAACGCTTTCTGATGCGTCTTTCTGCAGTGGCCGGGGTGC
TGTTTTACGTCGGCATGCTGACGGTGCATACGCCAGCGCTGCTGCTGGCGATGCAGGTGCTGAACGCCATCTATATCGGG
ATCCTCGCCGGGATCGGCATGCTCTATTTCCAGGACCTGATGCCGGGGCAGGCGGGCGCGGCCACCACCCTCTATACCAA
TACTACCCGCGTCGGCTGGATCATCGCCGGTTCACTGGCGGGGGTAGTGGCGGAGATCTGGAGCTATCACGCAGTGTTCT
GGATTGCGTTGGCGATGGGCGTTGTCACTCAGGCCTGCCTGTGGCGGATAAGAGACGTGTAA

Upstream 100 bases:

>100_bases
TTGAAACGCCTTTTTAATTATTCGTGCCATACTACTGTTTATTTTTAGCCAGCAGGCGTAAACTCCGCGCCGTCTCCTGA
AACTACAGAAAACCAAACCC

Downstream 100 bases:

>100_bases
GCACATTGGTTTCCATCCCTCTCATCTGCTGTGTTGAGCTCAGGGCGCGGTGGCCAGCTCAAGCTCCAGCAGCCAGGTGA
TCGCTTCATCGCGGTGGGTG

Product: putative transport

Products: lactose [Periplasm]; Proton [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 393; Mature: 393

Protein sequence:

>393_residues
MENRSAALPRRGFDVTSSAFLIVAFLTGIAGALQTPTLSLFLTNEVHVRPAMVGFFFTGSAVIGILVSQFLAGRSDRQGD
RKQLIVVCCLLGVLACVLFAWNRNYFILLFIGVFLSSFGSTANPQMFALAREHADRTGREAVMFSSILRAQVSLAWVIGP
PLAYALAMGFGFTAMYLSAAAAFIVCGIMVWLFLPSMRKENPVATGRLEAPRTHRRDALLLFSICTMMWGTNSLYIINMP
LFIINELHLPEKLAGLMMGTAAGLEIPTMLIAGYYARRFGKRFLMRLSAVAGVLFYVGMLTVHTPALLLAMQVLNAIYIG
ILAGIGMLYFQDLMPGQAGAATTLYTNTTRVGWIIAGSLAGVVAEIWSYHAVFWIALAMGVVTQACLWRIRDV

Sequences:

>Translated_393_residues
MENRSAALPRRGFDVTSSAFLIVAFLTGIAGALQTPTLSLFLTNEVHVRPAMVGFFFTGSAVIGILVSQFLAGRSDRQGD
RKQLIVVCCLLGVLACVLFAWNRNYFILLFIGVFLSSFGSTANPQMFALAREHADRTGREAVMFSSILRAQVSLAWVIGP
PLAYALAMGFGFTAMYLSAAAAFIVCGIMVWLFLPSMRKENPVATGRLEAPRTHRRDALLLFSICTMMWGTNSLYIINMP
LFIINELHLPEKLAGLMMGTAAGLEIPTMLIAGYYARRFGKRFLMRLSAVAGVLFYVGMLTVHTPALLLAMQVLNAIYIG
ILAGIGMLYFQDLMPGQAGAATTLYTNTTRVGWIIAGSLAGVVAEIWSYHAVFWIALAMGVVTQACLWRIRDV
>Mature_393_residues
MENRSAALPRRGFDVTSSAFLIVAFLTGIAGALQTPTLSLFLTNEVHVRPAMVGFFFTGSAVIGILVSQFLAGRSDRQGD
RKQLIVVCCLLGVLACVLFAWNRNYFILLFIGVFLSSFGSTANPQMFALAREHADRTGREAVMFSSILRAQVSLAWVIGP
PLAYALAMGFGFTAMYLSAAAAFIVCGIMVWLFLPSMRKENPVATGRLEAPRTHRRDALLLFSICTMMWGTNSLYIINMP
LFIINELHLPEKLAGLMMGTAAGLEIPTMLIAGYYARRFGKRFLMRLSAVAGVLFYVGMLTVHTPALLLAMQVLNAIYIG
ILAGIGMLYFQDLMPGQAGAATTLYTNTTRVGWIIAGSLAGVVAEIWSYHAVFWIALAMGVVTQACLWRIRDV

Specific function: Involved in the efflux of sugars. The physiological role may be the detoxification of non-metabolizable sugar analogs. Can transport lactose and glucose [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Set transporter family [H]

Homologues:

Organism=Escherichia coli, GI1788495, Length=393, Percent_Identity=81.9338422391858, Blast_Score=652, Evalue=0.0,
Organism=Escherichia coli, GI1790091, Length=393, Percent_Identity=67.6844783715013, Blast_Score=557, Evalue=1e-160,
Organism=Escherichia coli, GI48994875, Length=384, Percent_Identity=50.5208333333333, Blast_Score=389, Evalue=1e-109,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR004750 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 42924; Mature: 42924

Theoretical pI: Translated: 9.84; Mature: 9.84

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
5.1 %Met     (Translated Protein)
6.6 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
5.1 %Met     (Mature Protein)
6.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MENRSAALPRRGFDVTSSAFLIVAFLTGIAGALQTPTLSLFLTNEVHVRPAMVGFFFTGS
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEEECCEEECHHHHHHHHHHH
AVIGILVSQFLAGRSDRQGDRKQLIVVCCLLGVLACVLFAWNRNYFILLFIGVFLSSFGS
HHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEHHHHHHHHHHHCCC
TANPQMFALAREHADRTGREAVMFSSILRAQVSLAWVIGPPLAYALAMGFGFTAMYLSAA
CCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHEEECHHHHHHHHHHHHHHHHHHHHH
AAFIVCGIMVWLFLPSMRKENPVATGRLEAPRTHRRDALLLFSICTMMWGTNSLYIINMP
HHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEECH
LFIINELHLPEKLAGLMMGTAAGLEIPTMLIAGYYARRFGKRFLMRLSAVAGVLFYVGML
HHHHHHCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TVHTPALLLAMQVLNAIYIGILAGIGMLYFQDLMPGQAGAATTLYTNTTRVGWIIAGSLA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCEEHHEEHHHHH
GVVAEIWSYHAVFWIALAMGVVTQACLWRIRDV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MENRSAALPRRGFDVTSSAFLIVAFLTGIAGALQTPTLSLFLTNEVHVRPAMVGFFFTGS
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEEECCEEECHHHHHHHHHHH
AVIGILVSQFLAGRSDRQGDRKQLIVVCCLLGVLACVLFAWNRNYFILLFIGVFLSSFGS
HHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEHHHHHHHHHHHCCC
TANPQMFALAREHADRTGREAVMFSSILRAQVSLAWVIGPPLAYALAMGFGFTAMYLSAA
CCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHEEECHHHHHHHHHHHHHHHHHHHHH
AAFIVCGIMVWLFLPSMRKENPVATGRLEAPRTHRRDALLLFSICTMMWGTNSLYIINMP
HHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEECH
LFIINELHLPEKLAGLMMGTAAGLEIPTMLIAGYYARRFGKRFLMRLSAVAGVLFYVGML
HHHHHHCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TVHTPALLLAMQVLNAIYIGILAGIGMLYFQDLMPGQAGAATTLYTNTTRVGWIIAGSLA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCEEHHEEHHHHH
GVVAEIWSYHAVFWIALAMGVVTQACLWRIRDV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: lactose [Cytoplasm]; Proton [Periplasm] [C]

Specific reaction: lactose [Cytoplasm] + Proton [Periplasm] = lactose [Periplasm] + Proton [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9097040; 9278503; 10209755; 10438463 [H]